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The map label for this gene is yeiT [H]

Identifier: 157161626

GI number: 157161626

Start: 2276432

End: 2277670

Strand: Direct

Name: yeiT [H]

Synonym: EcHS_A2280

Alternate gene names: 157161626

Gene position: 2276432-2277670 (Clockwise)

Preceding gene: 157161625

Following gene: 157161627

Centisome position: 49.02

GC content: 51.49

Gene sequence:

>1239_bases
ATGCCGCAACAAAATTATCTGGATGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTT
ATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTC
GTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGTGCCGTTTGTGCCAGAGTGTGCCCGACGGAG
AAATTATGCCAAAGCGGTTGTACCCGTGCCGGTGTTGATGCTCCCATTGATATCGGCCGCTTACAGCGTTTTGTTACTGA
TTTTGAACAACAAACCGGAATGGAAATTTATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTC
CTGCCGGATTGCAGGCCAGTGTGACACTGACAAACCAGGGTTATGACGTCACGATTTATGAGAAAGAAGCGCACCCCGGT
GGCTGGTTGCGTAACGGTATTCCGCAATTCCGGTTACCGCAATCAGTGCTGGATGCAGAGATCGCCCGTATTGAAAAAAT
GGGCGTGACCATTAAGTGCAACAACGAAGTCGGTAACACACTCACCCTGGAGCAACTGAAAGCGGAAAACCGCGCGGTAC
TGGTCACCGTGGGGTTATCAAGCGGTTCCGGGCTACCGCTGTTTGAGCATAGCGACGTTGAGATTGCCGTCGACTTCTTG
CAACGTGCACGACAGGCGCAAGGCGATATCAGCATTCCACAAAGCGCATTAATTATCGGCGGCGGTGATGTCGCGATGGA
CGTAGCCAGCACGCTGAAAGTTCTCGGCTGTCAGGCGGTAACTTGCGTAGCGCGTGAAGAGTTAGATGAGTTTCCGGCAA
GCGAAAAAGAGTTTACCAGCGCCCGGGAGCTGGGCGTTTCGATCATCGATGGATTCACGCCAGTAGCCGTGGAAGGCAAT
AAAGTCACGTTTAAGCATGTACGGTTACCGGGCGAACTGACGATGGTGGCAGATAAAATTATTCTCGCCGTCGGTCAGCA
TGCCAGACTGGATGCCTTTGCGAAGTTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTATCAGACCCGCGATCCGC
AAGTCTTTGCTGCTGGCGATATTGTTGAGGGTGACAAAACCGTGGTCTATGCCGTGAAAACCGGGAAAGAAGCCGCCGAG
GCGATTCATCACTATTTAGAGGGAGCTTGCTCATGTTAA

Upstream 100 bases:

>100_bases
TTTCACAGTTTAAATTCCCCCGCACGCTTAGCCTTAATATCAGTACATTATTATTTACTAAACGCTCGCCTTAATTACCT
ATAGCATTAAGGAAGATCAC

Downstream 100 bases:

>100_bases
CGAAAGATCTTTCGATTACCTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGC
TATGAGATGTGTGCCAAAGC

Product: putative oxidoreductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 412; Mature: 411

Protein sequence:

>412_residues
MPQQNYLDELTPAFTSLLAIKEASRCLLCHDAPCSQACPAQTDPGKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTE
KLCQSGCTRAGVDAPIDIGRLQRFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEVGNTLTLEQLKAENRAVLVTVGLSSGSGLPLFEHSDVEIAVDFL
QRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLGCQAVTCVAREELDEFPASEKEFTSARELGVSIIDGFTPVAVEGN
KVTFKHVRLPGELTMVADKIILAVGQHARLDAFAKLEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAVKTGKEAAE
AIHHYLEGACSC

Sequences:

>Translated_412_residues
MPQQNYLDELTPAFTSLLAIKEASRCLLCHDAPCSQACPAQTDPGKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTE
KLCQSGCTRAGVDAPIDIGRLQRFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEVGNTLTLEQLKAENRAVLVTVGLSSGSGLPLFEHSDVEIAVDFL
QRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLGCQAVTCVAREELDEFPASEKEFTSARELGVSIIDGFTPVAVEGN
KVTFKHVRLPGELTMVADKIILAVGQHARLDAFAKLEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAVKTGKEAAE
AIHHYLEGACSC
>Mature_411_residues
PQQNYLDELTPAFTSLLAIKEASRCLLCHDAPCSQACPAQTDPGKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEK
LCQSGCTRAGVDAPIDIGRLQRFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPGG
WLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEVGNTLTLEQLKAENRAVLVTVGLSSGSGLPLFEHSDVEIAVDFLQ
RARQAQGDISIPQSALIIGGGDVAMDVASTLKVLGCQAVTCVAREELDEFPASEKEFTSARELGVSIIDGFTPVAVEGNK
VTFKHVRLPGELTMVADKIILAVGQHARLDAFAKLEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAVKTGKEAAEA
IHHYLEGACSC

Specific function: Unknown

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To E.coli glutamate synthase [NADPH] small chain (GltD) and to E.coli AegA [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=437, Percent_Identity=31.8077803203661, Blast_Score=180, Evalue=2e-45,
Organism=Homo sapiens, GI237757300, Length=90, Percent_Identity=48.8888888888889, Blast_Score=90, Evalue=3e-18,
Organism=Escherichia coli, GI1788468, Length=412, Percent_Identity=99.2718446601942, Blast_Score=842, Evalue=0.0,
Organism=Escherichia coli, GI1788811, Length=449, Percent_Identity=32.0712694877506, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI1789606, Length=442, Percent_Identity=31.6742081447964, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI87082180, Length=449, Percent_Identity=29.1759465478842, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1789243, Length=400, Percent_Identity=29.75, Blast_Score=141, Evalue=9e-35,
Organism=Caenorhabditis elegans, GI71984108, Length=441, Percent_Identity=33.1065759637188, Blast_Score=174, Evalue=7e-44,
Organism=Caenorhabditis elegans, GI17570289, Length=449, Percent_Identity=27.6169265033408, Blast_Score=145, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6320030, Length=461, Percent_Identity=25.1626898047722, Blast_Score=124, Evalue=4e-29,
Organism=Drosophila melanogaster, GI24640763, Length=434, Percent_Identity=31.3364055299539, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI18858217, Length=434, Percent_Identity=31.3364055299539, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI28574881, Length=446, Percent_Identity=28.47533632287, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI24665539, Length=446, Percent_Identity=28.47533632287, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI24665547, Length=444, Percent_Identity=28.1531531531532, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI24665543, Length=444, Percent_Identity=28.1531531531532, Blast_Score=135, Evalue=6e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: NA

Molecular weight: Translated: 44367; Mature: 44236

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQQNYLDELTPAFTSLLAIKEASRCLLCHDAPCSQACPAQTDPGKFIRSIYFRNFKGAA
CCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCHHH
ETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQRFVTDFEQQTGMEIYQPG
HHHHCCCCHHHHHHHHCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEECCC
TKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPGGWLRNGIPQFRLPQSVLDAE
CCCCCCEEEEECCCCCCEEEEEEECCCEEEEEEECCCCCCCHHHCCCCCCCCCHHHHHHH
IARIEKMGVTIKCNNEVGNTLTLEQLKAENRAVLVTVGLSSGSGLPLFEHSDVEIAVDFL
HHHHHHHCEEEEECCCCCCEEEHHHHHCCCCEEEEEEEECCCCCCCEEECCCHHHHHHHH
QRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLGCQAVTCVAREELDEFPASEKEFTS
HHHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
ARELGVSIIDGFTPVAVEGNKVTFKHVRLPGELTMVADKIILAVGQHARLDAFAKLEPQR
HHHHCHHHHCCCCCEEEECCEEEEEEEECCCHHHHHHHHHHHHCCCCCCCCHHHCCCCCC
NTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAVKTGKEAAEAIHHYLEGACSC
CCCCCCCCCCCCCEEEEECCEECCCCEEEEEEECCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PQQNYLDELTPAFTSLLAIKEASRCLLCHDAPCSQACPAQTDPGKFIRSIYFRNFKGAA
CCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCHHH
ETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQRFVTDFEQQTGMEIYQPG
HHHHCCCCHHHHHHHHCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEECCC
TKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPGGWLRNGIPQFRLPQSVLDAE
CCCCCCEEEEECCCCCCEEEEEEECCCEEEEEEECCCCCCCHHHCCCCCCCCCHHHHHHH
IARIEKMGVTIKCNNEVGNTLTLEQLKAENRAVLVTVGLSSGSGLPLFEHSDVEIAVDFL
HHHHHHHCEEEEECCCCCCEEEHHHHHCCCCEEEEEEEECCCCCCCEEECCCHHHHHHHH
QRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLGCQAVTCVAREELDEFPASEKEFTS
HHHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
ARELGVSIIDGFTPVAVEGNKVTFKHVRLPGELTMVADKIILAVGQHARLDAFAKLEPQR
HHHHCHHHHCCCCCEEEECCEEEEEEEECCCHHHHHHHHHHHHCCCCCCCCHHHCCCCCC
NTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAVKTGKEAAEAIHHYLEGACSC
CCCCCCCCCCCCCEEEEECCEECCCCEEEEEEECCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]