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The map label for this gene is insB1 [H]

Identifier: 157161552

GI number: 157161552

Start: 2178702

End: 2179205

Strand: Direct

Name: insB1 [H]

Synonym: EcHS_A2198

Alternate gene names: 157161552

Gene position: 2178702-2179205 (Clockwise)

Preceding gene: 157161551

Following gene: 157161559

Centisome position: 46.92

GC content: 52.58

Gene sequence:

>504_bases
ATGTCGCGCCAGTGCACGCATTATGGGCGTTGGCCTCAACACGGTTTTACGTCACTTAAAAAACTCAGGCCGCAGTCGGT
AACCTCGCGCATACAACCGGGCAGTGATGTGATTGTCTGCGCTGAAATGGACGAACAGTGGGGCTACGTCGGCGCTAAAT
CACGCCAGCGCTGGTTGTTTTACGCGTATGACAGGATGCGCAGGACAGTTGTGGCCCATGTATTCAGAGAACGCACTATG
ACCACGCTGGAGCGTCTTCTGAGCCTGTTGTCAGTCTTTGAAGTCGTGATATGGATGACGGACGGATGGTCGCTGTATGA
GTCACGGCTTAAGGAGAGGCTGCACGTTATCAGCAAGCGTTACACGCAGCGCATTGAGCGGCATAACCTGAATCTGAGGC
AGCATCTGGCCCGGCTGGGAAGGAAGTCACTGTCGTTCTCAAAATCGGTGGAACTGCATGAAAAGGTCATCGGGCATTAT
CTGAACATCAAACACTATCAGTAA

Upstream 100 bases:

>100_bases
GCTCTCACTGCCGTAAAACATGGCAGCTACAGTTCACTTACACCGCTTCTCAACCCGGTACGCATCAGAAAATCATTGAT
ATGGCCATGAATGGCGTCGG

Downstream 100 bases:

>100_bases
GTTGGAGCCACCACCCATTATTTCATAAATATGAATTATCAATTACCGAGTATATAGTAGATTATTTTCTTATTTGTCTA
ATTAACCGGCGAAAACTTAA

Product: IS1, transposase orfB

Products: NA

Alternate protein names: IS1a [H]

Number of amino acids: Translated: 167; Mature: 166

Protein sequence:

>167_residues
MSRQCTHYGRWPQHGFTSLKKLRPQSVTSRIQPGSDVIVCAEMDEQWGYVGAKSRQRWLFYAYDRMRRTVVAHVFRERTM
TTLERLLSLLSVFEVVIWMTDGWSLYESRLKERLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHEKVIGHY
LNIKHYQ

Sequences:

>Translated_167_residues
MSRQCTHYGRWPQHGFTSLKKLRPQSVTSRIQPGSDVIVCAEMDEQWGYVGAKSRQRWLFYAYDRMRRTVVAHVFRERTM
TTLERLLSLLSVFEVVIWMTDGWSLYESRLKERLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHEKVIGHY
LNIKHYQ
>Mature_166_residues
SRQCTHYGRWPQHGFTSLKKLRPQSVTSRIQPGSDVIVCAEMDEQWGYVGAKSRQRWLFYAYDRMRRTVVAHVFRERTMT
TLERLLSLLSVFEVVIWMTDGWSLYESRLKERLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHEKVIGHYL
NIKHYQ

Specific function: Absolutely required for transposition of IS1 [H]

COG id: COG1662

COG function: function code L; Transposase and inactivated derivatives, IS1 family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 27 family [H]

Homologues:

Organism=Escherichia coli, GI1789853, Length=167, Percent_Identity=88.622754491018, Blast_Score=309, Evalue=7e-86,
Organism=Escherichia coli, GI1788203, Length=167, Percent_Identity=88.622754491018, Blast_Score=309, Evalue=7e-86,
Organism=Escherichia coli, GI1786203, Length=167, Percent_Identity=88.622754491018, Blast_Score=309, Evalue=7e-86,
Organism=Escherichia coli, GI1787223, Length=167, Percent_Identity=86.8263473053892, Blast_Score=304, Evalue=2e-84,
Organism=Escherichia coli, GI1786470, Length=167, Percent_Identity=86.8263473053892, Blast_Score=302, Evalue=7e-84,
Organism=Escherichia coli, GI1786459, Length=167, Percent_Identity=86.8263473053892, Blast_Score=302, Evalue=7e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005063 [H]

Pfam domain/function: PF03400 Transposase_27 [H]

EC number: NA

Molecular weight: Translated: 20049; Mature: 19917

Theoretical pI: Translated: 10.85; Mature: 10.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRQCTHYGRWPQHGFTSLKKLRPQSVTSRIQPGSDVIVCAEMDEQWGYVGAKSRQRWLF
CCCCCCCCCCCCCHHHHHHHHHCHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCHHHH
YAYDRMRRTVVAHVFRERTMTTLERLLSLLSVFEVVIWMTDGWSLYESRLKERLHVISKR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
YTQRIERHNLNLRQHLARLGRKSLSFSKSVELHEKVIGHYLNIKHYQ
HHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SRQCTHYGRWPQHGFTSLKKLRPQSVTSRIQPGSDVIVCAEMDEQWGYVGAKSRQRWLF
CCCCCCCCCCCCHHHHHHHHHCHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCHHHH
YAYDRMRRTVVAHVFRERTMTTLERLLSLLSVFEVVIWMTDGWSLYESRLKERLHVISKR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
YTQRIERHNLNLRQHLARLGRKSLSFSKSVELHEKVIGHYLNIKHYQ
HHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 375010; 1849492; 1630901; 9278503 [H]