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The map label for this gene is add

Identifier: 157161084

GI number: 157161084

Start: 1724186

End: 1725187

Strand: Direct

Name: add

Synonym: EcHS_A1698

Alternate gene names: 157161084

Gene position: 1724186-1725187 (Clockwise)

Preceding gene: 157161083

Following gene: 157161086

Centisome position: 37.13

GC content: 53.89

Gene sequence:

>1002_bases
ATGATTGATACCACCCTGCCATTAACTGATATCCATCGCCACCTTGATGGCAACATTCGTCCCCAGACCATTCTTGAACT
TGGCCGCCAGTATAATATCTCGCTTCCTGCACAATCCCTGGAAACACTGATTCCCCACGTTCAGGTCATTGCCAACGAAC
CCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCA
TTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGC
ACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGCACCTTTGGTGTGCAGG
CGAAGCTTATCGGCATTATGAGCCGGACCTTCGGCGAAGCCGCCTGTCAGCAAGAGCTGGAGGCCTTTTTAGCCCACCGT
GACCAGATTACCGCACTTGATTTAGCCGGTGATGAACTTGGTTTCCCGGGAAGTCTGTTCCTTTCTCACTTCAACCGCGC
GCGTGATGCGGGCTGGCATATTACCGTCCATGCAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTCGTGAAC
TGGGTGCGGAGCGTATTGGACATGGCGTAAAAGCCATTGAAGATCGGGCGCTGATGGATTTTCTCGCCGAGCAACAAATT
GGTATTGAATCCTGTCTGACCTCCAATATTCAGACCAGCACCGTAGCAGAGCTGGCTGCACATCCGCTGAAAACGTTCCT
TGAGCATGGCATTCGTGCCAGCATTAACACTGACGATCCCGGCGTACAGGGAGTGGATATCATTCACGAATATACCGTTG
CCGCGCCAGCTGCTGGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCT
GAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAA

Upstream 100 bases:

>100_bases
CAATTGCGCAACGTAAAAAATCGTTGCGCAATCGTGGATTTTTACCCTGCTTTGTTTTTATAATGGTGCGCACTTTTATA
TCCAGAAAAAGAGTGCGACC

Downstream 100 bases:

>100_bases
CAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCA
GCTCAATCAACTCCATTACC

Product: adenosine deaminase

Products: NA

Alternate protein names: Adenosine aminohydrolase

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLIPHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVA
FENIEDAARHGLHYVELRFSPGYMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAERIGHGVKAIEDRALMDFLAEQQI
GIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASINTDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSA
EEKRALREKVAAK

Sequences:

>Translated_333_residues
MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLIPHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVA
FENIEDAARHGLHYVELRFSPGYMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAERIGHGVKAIEDRALMDFLAEQQI
GIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASINTDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSA
EEKRALREKVAAK
>Mature_333_residues
MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLIPHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVA
FENIEDAARHGLHYVELRFSPGYMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAERIGHGVKAIEDRALMDFLAEQQI
GIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASINTDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSA
EEKRALREKVAAK

Specific function: Unknown

COG id: COG1816

COG function: function code F; Adenosine deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenosine and AMP deaminases family

Homologues:

Organism=Homo sapiens, GI47078295, Length=339, Percent_Identity=33.0383480825959, Blast_Score=160, Evalue=2e-39,
Organism=Escherichia coli, GI1787910, Length=333, Percent_Identity=100, Blast_Score=679, Evalue=0.0,
Organism=Caenorhabditis elegans, GI32565969, Length=339, Percent_Identity=30.0884955752212, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17538512, Length=339, Percent_Identity=30.0884955752212, Blast_Score=129, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6324188, Length=338, Percent_Identity=26.6272189349112, Blast_Score=105, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24645260, Length=335, Percent_Identity=26.865671641791, Blast_Score=110, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ADD_ECO24 (A7ZM83)

Other databases:

- EMBL:   CP000800
- RefSeq:   YP_001462912.1
- ProteinModelPortal:   A7ZM83
- SMR:   A7ZM83
- STRING:   A7ZM83
- EnsemblBacteria:   EBESCT00000021859
- GeneID:   5587676
- GenomeReviews:   CP000800_GR
- KEGG:   ecw:EcE24377A_1831
- eggNOG:   COG1816
- GeneTree:   EBGT00050000009969
- HOGENOM:   HBG630382
- OMA:   PYYMAMN
- ProtClustDB:   PRK09358
- BioCyc:   ECOL331111:ECE24377A_1831-MONOMER
- HAMAP:   MF_00540
- InterPro:   IPR006650
- InterPro:   IPR001365
- InterPro:   IPR006330
- PANTHER:   PTHR11409
- TIGRFAMs:   TIGR01430

Pfam domain/function: PF00962 A_deaminase

EC number: =3.5.4.4

Molecular weight: Translated: 36398; Mature: 36398

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS00485 A_DEAMINASE

Important sites: ACT_SITE 200-200 BINDING 14-14 BINDING 16-16 BINDING 170-170 BINDING 279-279

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLIPHVQVIANEPDLVSFL
CCCCCCCHHHHHHHHCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHH
TKLDWGVKVLASLDACRRVAFENIEDAARHGLHYVELRFSPGYMAMAHQLPVAGVVEAVI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHCCHHHHHHHHH
DGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHRDQITALDLAGDELGFPGSLF
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHH
LSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAERIGHGVKAIEDRALMDFLAEQQI
HHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHC
GIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASINTDDPGVQGVDIIHEYTVAAPAAGL
CHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHHHHHCCCCCC
SREQIRQAQINGLEMAFLSAEEKRALREKVAAK
CHHHHHHHHHCCHHHHHHCHHHHHHHHHHHCCC
>Mature Secondary Structure
MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLIPHVQVIANEPDLVSFL
CCCCCCCHHHHHHHHCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHH
TKLDWGVKVLASLDACRRVAFENIEDAARHGLHYVELRFSPGYMAMAHQLPVAGVVEAVI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHCCHHHHHHHHH
DGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHRDQITALDLAGDELGFPGSLF
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHH
LSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAERIGHGVKAIEDRALMDFLAEQQI
HHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHC
GIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASINTDDPGVQGVDIIHEYTVAAPAAGL
CHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHHHHHCCCCCC
SREQIRQAQINGLEMAFLSAEEKRALREKVAAK
CHHHHHHHHHCCHHHHHHCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA