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The map label for this gene is manA [H]

Identifier: 157161074

GI number: 157161074

Start: 1710710

End: 1711885

Strand: Direct

Name: manA [H]

Synonym: EcHS_A1688

Alternate gene names: 157161074

Gene position: 1710710-1711885 (Clockwise)

Preceding gene: 157161071

Following gene: 157161075

Centisome position: 36.84

GC content: 50.94

Gene sequence:

>1176_bases
ATGCAAAAACTCATTAACTCAGTGCAAAACTATGCCTGGGGCAGCAAAACGGCGTTGACTGAACTTTATGGTATGGAAAA
TCCGTCCAGCCAGCCGATGGCCGAGCTGTGGATGGGCGCACATCCGAAAAGCAGTTCACGAGTGCAGAATGCCGCCGGAG
ATATCGTTTCACTGCGTGATGTGATTGAGAGTGATAAATCGACTCTGCTCGGAGAGGCCGTTGCCAAACGCTTTGGCGAA
CTGCCTTTCCTGTTCAAAGTATTATGCGCAGCACAGCCGCTCTCCATTCAGGTTCATCCAAACAAACGCAATTCTGAAAT
CGGTTTTGCCAAAGAAAATGCCGCAGGTATCCCGATGGATGCCGCCGAGCGTAACTATAAAGATCCTAACCACAAGCCGG
AGCTGGTTTTTGCGCTGACGCCTTTCCTTGCGATGAACGCGTTTCGTGAGTTTTCCGAGATTGTCTCCCTACTCCAGCCA
GTCGCAGGTGCACATCCGGCAATTGCTCACTTTTTACAACAGCCTAATGCCGAACGTTTAAGCGAACTGTTCGCCAGCCT
GTTGAATATGCAGGGTGAAGAAAAATCCCGCGCGCTGGCGATTTTAAAATCGGCGCTCGACAGTCAGCAGGGTGAACCGT
GGCAAACGATTCGTTTAATTTCTGAATTTTACCCGGAAGACAGCGGCCTGTTCTCCCCGCTGTTACTGAATGTGGTGAAA
TTGAATCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCACGCTTACCTGCAAGGCGTGGCGCTGGAAGTGATGGC
GAACTCCGATAACGTGCTGCGTGCGGGTCTGACGCCTAAGTACATTGATATTCCGGAACTGGTTGCCAATGTGAAATTCG
AAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGAT
TTTGCCTTCTCGCTGCATGACCTTAGTGATAAAGAAACCACCATTAGCCAGCAGAGTGCCGCCATTTTGTTCTGCGTCGA
AGGCGATGCAACGTTGTGGAAAGGTTCTCAGCAGTTACAGCTTAAACCGGGTGAATCAGCGTTTATTGCCGCCAACGAAT
CACCGGTGACTGTCAAAGGCCACGGCCGTTTAGCGCGTGTTTACAACAAGCTGTAA

Upstream 100 bases:

>100_bases
AAACTGAGACTAGTACGACTTTTTGCGGCTCCAGGTTACTTCCCGTAGGATTCTTGCTTTAATAGTGGGATTAATTTCCA
CATTAAAACAGGGATTGATC

Downstream 100 bases:

>100_bases
GAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATT
TCATTTTATGGATAATCATT

Product: mannose-6-phosphate isomerase

Products: NA

Alternate protein names: Phosphohexomutase; Phosphomannose isomerase; PMI [H]

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKSSSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGE
LPFLFKVLCAAQPLSIQVHPNKRNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIRLISEFYPEDSGLFSPLLLNVVK
LNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAGLTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDD
FAFSLHDLSDKETTISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL

Sequences:

>Translated_391_residues
MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKSSSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGE
LPFLFKVLCAAQPLSIQVHPNKRNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIRLISEFYPEDSGLFSPLLLNVVK
LNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAGLTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDD
FAFSLHDLSDKETTISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL
>Mature_391_residues
MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKSSSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGE
LPFLFKVLCAAQPLSIQVHPNKRNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIRLISEFYPEDSGLFSPLLLNVVK
LNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAGLTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDD
FAFSLHDLSDKETTISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL

Specific function: Involved in the conversion of glucose to GDP-L-fucose, which can be converted to L-fucose, a capsular polysaccharide [H]

COG id: COG1482

COG function: function code G; Phosphomannose isomerase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mannose-6-phosphate isomerase type 1 family [H]

Homologues:

Organism=Homo sapiens, GI4505235, Length=406, Percent_Identity=34.2364532019704, Blast_Score=187, Evalue=1e-47,
Organism=Escherichia coli, GI1787899, Length=391, Percent_Identity=99.4884910485933, Blast_Score=795, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71997620, Length=394, Percent_Identity=31.2182741116751, Blast_Score=165, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI17557650, Length=420, Percent_Identity=29.0476190476191, Blast_Score=132, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6320839, Length=419, Percent_Identity=31.7422434367542, Blast_Score=178, Evalue=2e-45,
Organism=Drosophila melanogaster, GI21356061, Length=400, Percent_Identity=31.75, Blast_Score=161, Evalue=7e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011051
- InterPro:   IPR001250
- InterPro:   IPR016305
- InterPro:   IPR018050
- InterPro:   IPR014710 [H]

Pfam domain/function: PF01238 PMI_typeI [H]

EC number: =5.3.1.8 [H]

Molecular weight: Translated: 42868; Mature: 42868

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS00965 PMI_I_1 ; PS00966 PMI_I_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKSSSRVQNAAGDIVSLRD
CHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCHHHHHCCCCCCCHHHHHHHHCCHHHHHH
VIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNKRNSEIGFAKENAAGIPMD
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCCC
AAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQPVAGAHPAIAHFLQQPNAERL
HHHCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHH
SELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIRLISEFYPEDSGLFSPLLLNVVK
HHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHE
LNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAGLTPKYIDIPELVANVKFEAKPANQ
ECCCCEEEEEECCCHHHHCCEEEEEEECCCCEEECCCCCCCCCHHHHHHCCCCCCCCHHH
LLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETTISQQSAAILFCVEGDATLWKGSQQLQ
HHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCHHHHCCCCCEEEEEECCCCCEECCCCEEE
LKPGESAFIAANESPVTVKGHGRLARVYNKL
ECCCCCEEEEECCCCEEEECCCHHHHHHHCC
>Mature Secondary Structure
MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKSSSRVQNAAGDIVSLRD
CHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCHHHHHCCCCCCCHHHHHHHHCCHHHHHH
VIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNKRNSEIGFAKENAAGIPMD
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCCC
AAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQPVAGAHPAIAHFLQQPNAERL
HHHCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHH
SELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIRLISEFYPEDSGLFSPLLLNVVK
HHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHE
LNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAGLTPKYIDIPELVANVKFEAKPANQ
ECCCCEEEEEECCCHHHHCCEEEEEEECCCCEEECCCCCCCCCHHHHHHCCCCCCCCHHH
LLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETTISQQSAAILFCVEGDATLWKGSQQLQ
HHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCHHHHCCCCCEEEEEECCCCCEECCCCEEE
LKPGESAFIAANESPVTVKGHGRLARVYNKL
ECCCCCEEEEECCCCEEEECCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6397402; 9097039; 9278503 [H]