Click here to switch to the map view.

The map label for this gene is pqqL [H]

Identifier: 157160970

GI number: 157160970

Start: 1589233

End: 1592013

Strand: Reverse

Name: pqqL [H]

Synonym: EcHS_A1579

Alternate gene names: 157160970

Gene position: 1592013-1589233 (Counterclockwise)

Preceding gene: 157160971

Following gene: 157160969

Centisome position: 34.28

GC content: 46.6

Gene sequence:

>2781_bases
ATGAGAAACCTCTGTTTCTTACTGACATTAGTGGCAACTCTGTTGCTCCCAGGGCGGCTGATTGCCGCCTTACCGCAGGA
TGAAAAGTTAATTACCGGGCAACTGGACAATGGCTTGCGATATATGATTTATCCGCATGCTCATCCAAAGGATCAGGTAA
ATTTATGGCTGCAAATTCATACCGGTTCATTGCAGGAAGAAGACAATGAGCGCGGCGTGGCTCATTTTGTAGAACATATG
ATGTTTAACGGCACAAAAACATGGCCGGGTAATAAAGTCATCGAAACATTTGAGTCAATGGGCCTGCGTTTTGGTCGCGA
TGTTAATGCCTATACCAGCTATGACGAAACGGTGTATCAGGTGAGTTTGCCGACTACGCAGAAACAAAATCTGCAACAAG
TGATGGCAATCTTCAGTGAATGGAGTAATGCCGCAAGCTTTGAAAAACTCGAAGTAGACGCTGAACGCGGCGTAATTACT
GAGGAATGGCGTGCCCATCAGGATGCGAAATGGCGCACCTCTCAGGCGCGCCGCCCTTTCCTGCTGGCTAATACCCGTAA
TTTAGACCGTGAACCTATCGGCCTGATGGATACTGTCGCCACGGTCACACCGGCACAATTGCGCCAATTTTATCAACGCT
GGTATCAACCAAATAATATGACCTTTATCGTGGTTGGCGATATCGACAGTAAAGAAGCGCTGGCGCTGATAAAGGATAAT
TTAAGTAAGCTTCCGGCTAACAAAGCAGCTGAAAATCGCGTCTGGCCGACAAAAGCCGAAAATCACCTGCGCTTTAATAT
CATTAATGATAAAGAAAACCGGGTGAACGGCATCGCACTCTATTATCGCCTGCCAATGGTGCAAGTGAGCGATGAGCAAA
GCTTTATCGAACAAGCTGAATGGAGCATGTTAGTTCAGCTGTTCAATCAACGTCTGCAGGAACGCATACAATCGGGCGAG
TTGAAGACTATTTCTGGCGGTACTGCACGCAGCGTTAAAATTGCACCCGATTATCAGTCGCTGTTTTTCCGTGTAAATGC
ACGAGGCGATAATATGCAGGATGCTGCGAATGCATTAATGGCAGAGTTGGCAACCATTGATCAGCATGGTTTTTCTGCTG
AAGAACTCGATGATGTCAAATCTACCCGCCTCACCTGGCTGAAAAATGCGGTTGATCAGCAAGCTGAACGTGATTTACGT
ATGCTGACCAGTCGCCTGGCATCCAGCTCATTAAATAATACGCCGTTCTTGTCGCCGGAAGAGACATATCAACTTTCGAA
ACGTCTGTGGCAGCAAATTACCGTGCAAAGTCTGGCGGAAAAATGGCAGCAGTTAAGAAAGAACCAGGACGCATTTTGGG
AGCAAATGGTAAACAATGAGCTTGCCGCCAAAAAAGCATTGTCTCCTGCGGCTATCCTGGCGCTGGAAAAAGAGTACGCC
AACAAAAAGCTGGCGGCTTACATCTTCCCAGGCAGAAATTTATCGTTAACAGTAGACGCTGACCCACAGGCGGAAATTAG
CAGCAAAGAAACGCTGGCTGAGAATCTGACATCATTAACACTTTCCAATGGTGCAAGGGTTATTCTGGCAAAATCCGCGG
GTGAAGAGCAAAAGCTACAAATTATTGCCGTATCGAATAAAGGCGATTTAAGTTTCCCTGCGCAGCAAAAATCACTTATC
GCGCTGGCAAATAAAGCAGTTAGCGGAAGCGGCGTTGGCGAACTCTCCTCTTCCAGCCTGAAACGCTGGAGTGCGGAAAA
CTCGGTAACCATGAGCAGTAAAGTCAGTGGCATGAATACGTTGCTCTCCGTTAGCGCGCGGACTAATAACCCTGAACCTG
GTTTCCAGTTGATTAACCAGCGAATCACCCACAGCACGATTAACGACAATATTTGGGCATCGCTACAAAATGCTCAAATT
CAGGCGTTGAAAACGCTCGACCAGCGTCCAGCGGAGAAATTCGCCCAGCAGATGTATGAGACGCGCTATGCTGATGACCG
CACGAAATTACTGCAAGAAAATCAGATTGCACAGTTTACTGCCGCAGATGCGCTGGCTGCCGATCGCCAGTTGTTTTCAT
CTCCAGCGGATATCACGTTTGTCATTGTCGGTAATGTCTCAGAAGACAAACTCGTGGCGTTAATTACGCGTTACTTAGGA
TCAATCAAACACTCTGATTCGCCATTAGCCGCAGGTAAACCATTAACTCGCGCGACGGACAACGCATCGGTTACTGTAAA
AGAACAAAATGAACCTGTGGCACAGGTTTCACAGTGGAAGCGTTATGATTCCCGGACACCTGTTAATCTGGCGACGCGTA
TGGCGCTCGATGCTTTTAACGTCGCACTGGCAAAAGATCTACGTGTTAATATTCGTGAACAGGCATCTGGAGCATACAGC
GTTTCTTCTCGCCTCTCGGTTGATCCTCAGGCCAAAGATATCAGTCATTTGCTGGCTTTTACTTGTCAACCAGAACGACA
TGATGAACTGTTAACGTTAGCGAATGAAGTGATGGTTAAGCGTCTGGCTAAAGGGATCAGTGAGCAAGAACTGAATGAAT
ACCAGCAAAACGTTCAGCGCAGCCTCGATATCCAACAGCGTAGCGTTCAACAATTAGCGAACACCATTGTAAATAGTCTT
ATTCAATATGACGATCCTGCAGCATGGACTGAGCAGGAGCAATTGTTGAAACAAATGACGGTAGAGAATGTTAACACTGC
CGTTAAACAATATCTTTCTCATCCGGTAAATACTTATACCGGAGTATTATTGCCAAAATAA

Upstream 100 bases:

>100_bases
CCAGTGGCCGTACTTTCTGGCTTGATGTCAGCATGAAATTTTAATAGTTCGGATTTTACTTAACCGGGTAAATTCGCCCG
GTTTTCGCATGGAGATAATT

Downstream 100 bases:

>100_bases
TAACAGCCCCGTCAACACATCGTTGGCGGGAATTTTAGCAATATTCGCCATTTTTATGTAATAATTTTATAAATGCGTTC
AAAATAATAACTAAGTACTA

Product: M16B family peptidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 926; Mature: 926

Protein sequence:

>926_residues
MRNLCFLLTLVATLLLPGRLIAALPQDEKLITGQLDNGLRYMIYPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHM
MFNGTKTWPGNKVIETFESMGLRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAASFEKLEVDAERGVIT
EEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQPNNMTFIVVGDIDSKEALALIKDN
LSKLPANKAAENRVWPTKAENHLRFNIINDKENRVNGIALYYRLPMVQVSDEQSFIEQAEWSMLVQLFNQRLQERIQSGE
LKTISGGTARSVKIAPDYQSLFFRVNARGDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAVDQQAERDLR
MLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQDAFWEQMVNNELAAKKALSPAAILALEKEYA
NKKLAAYIFPGRNLSLTVDADPQAEISSKETLAENLTSLTLSNGARVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLI
ALANKAVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRITHSTINDNIWASLQNAQI
QALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIAQFTAADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLG
SIKHSDSPLAAGKPLTRATDNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASGAYS
VSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQQNVQRSLDIQQRSVQQLANTIVNSL
IQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLSHPVNTYTGVLLPK

Sequences:

>Translated_926_residues
MRNLCFLLTLVATLLLPGRLIAALPQDEKLITGQLDNGLRYMIYPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHM
MFNGTKTWPGNKVIETFESMGLRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAASFEKLEVDAERGVIT
EEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQPNNMTFIVVGDIDSKEALALIKDN
LSKLPANKAAENRVWPTKAENHLRFNIINDKENRVNGIALYYRLPMVQVSDEQSFIEQAEWSMLVQLFNQRLQERIQSGE
LKTISGGTARSVKIAPDYQSLFFRVNARGDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAVDQQAERDLR
MLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQDAFWEQMVNNELAAKKALSPAAILALEKEYA
NKKLAAYIFPGRNLSLTVDADPQAEISSKETLAENLTSLTLSNGARVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLI
ALANKAVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRITHSTINDNIWASLQNAQI
QALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIAQFTAADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLG
SIKHSDSPLAAGKPLTRATDNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASGAYS
VSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQQNVQRSLDIQQRSVQQLANTIVNSL
IQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLSHPVNTYTGVLLPK
>Mature_926_residues
MRNLCFLLTLVATLLLPGRLIAALPQDEKLITGQLDNGLRYMIYPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHM
MFNGTKTWPGNKVIETFESMGLRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAASFEKLEVDAERGVIT
EEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQPNNMTFIVVGDIDSKEALALIKDN
LSKLPANKAAENRVWPTKAENHLRFNIINDKENRVNGIALYYRLPMVQVSDEQSFIEQAEWSMLVQLFNQRLQERIQSGE
LKTISGGTARSVKIAPDYQSLFFRVNARGDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAVDQQAERDLR
MLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQDAFWEQMVNNELAAKKALSPAAILALEKEYA
NKKLAAYIFPGRNLSLTVDADPQAEISSKETLAENLTSLTLSNGARVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLI
ALANKAVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRITHSTINDNIWASLQNAQI
QALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIAQFTAADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLG
SIKHSDSPLAAGKPLTRATDNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASGAYS
VSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQQNVQRSLDIQQRSVQQLANTIVNSL
IQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLSHPVNTYTGVLLPK

Specific function: Unknown

COG id: COG0612

COG function: function code R; Predicted Zn-dependent peptidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M16 family [H]

Homologues:

Organism=Homo sapiens, GI46593007, Length=216, Percent_Identity=25.462962962963, Blast_Score=74, Evalue=7e-13,
Organism=Homo sapiens, GI155969707, Length=356, Percent_Identity=25, Blast_Score=68, Evalue=5e-11,
Organism=Escherichia coli, GI1787770, Length=927, Percent_Identity=99.0291262135922, Blast_Score=1865, Evalue=0.0,
Organism=Escherichia coli, GI1789946, Length=444, Percent_Identity=20.4954954954955, Blast_Score=75, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71982342, Length=253, Percent_Identity=28.4584980237154, Blast_Score=72, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324474, Length=192, Percent_Identity=26.0416666666667, Blast_Score=71, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011249
- InterPro:   IPR011237
- InterPro:   IPR011765
- InterPro:   IPR001431
- InterPro:   IPR007863 [H]

Pfam domain/function: PF00675 Peptidase_M16; PF05193 Peptidase_M16_C [H]

EC number: 3.4.99.- [C]

Molecular weight: Translated: 104062; Mature: 104062

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS00143 INSULINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNLCFLLTLVATLLLPGRLIAALPQDEKLITGQLDNGLRYMIYPHAHPKDQVNLWLQIH
CCHHHHHHHHHHHHHCCCHHHHHCCCCCCEEECCCCCCCEEEEECCCCCCCCEEEEEEEE
TGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMGLRFGRDVNAYTSYDETVYQ
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCHHCCCCCCCCCCCCCEEEE
VSLPTTQKQNLQQVMAIFSEWSNAASFEKLEVDAERGVITEEWRAHQDAKWRTSQARRPF
EECCCCCHHHHHHHHHHHHHHCCCCCCHHEEECHHCCCCCHHHHCCCCCCHHHHHCCCCE
LLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQPNNMTFIVVGDIDSKEALALIKDN
EEECCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHH
LSKLPANKAAENRVWPTKAENHLRFNIINDKENRVNGIALYYRLPMVQVSDEQSFIEQAE
HHHCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCEEEEEEECEEEECCHHHHHHHHH
WSMLVQLFNQRLQERIQSGELKTISGGTARSVKIAPDYQSLFFRVNARGDNMQDAANALM
HHHHHHHHHHHHHHHHHCCCEEEECCCCCCEEEECCCHHHEEEEEECCCCCHHHHHHHHH
AELATIDQHGFSAEELDDVKSTRLTWLKNAVDQQAERDLRMLTSRLASSSLNNTPFLSPE
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCH
ETYQLSKRLWQQITVQSLAEKWQQLRKNQDAFWEQMVNNELAAKKALSPAAILALEKEYA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHEEHHHHC
NKKLAAYIFPGRNLSLTVDADPQAEISSKETLAENLTSLTLSNGARVILAKSAGEEQKLQ
CCEEEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHEEECCCCEEEEEECCCCCCEEE
IIAVSNKGDLSFPAQQKSLIALANKAVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNT
EEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCEEEHHHHCCHHH
LLSVSARTNNPEPGFQLINQRITHSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYE
HEEEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHCHHHHHHHHHHHCCHHHHHHHHHHH
TRYADDRTKLLQENQIAQFTAADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLG
HHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHH
SIKHSDSPLAAGKPLTRATDNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFN
HCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHH
VALAKDLRVNIREQASGAYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVK
HHHHHHHHHHHHHHCCCCEECCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
RLAKGISEQELNEYQQNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMT
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
VENVNTAVKQYLSHPVNTYTGVLLPK
HHHHHHHHHHHHCCCCHHHCCCEECC
>Mature Secondary Structure
MRNLCFLLTLVATLLLPGRLIAALPQDEKLITGQLDNGLRYMIYPHAHPKDQVNLWLQIH
CCHHHHHHHHHHHHHCCCHHHHHCCCCCCEEECCCCCCCEEEEECCCCCCCCEEEEEEEE
TGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMGLRFGRDVNAYTSYDETVYQ
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCHHCCCCCCCCCCCCCEEEE
VSLPTTQKQNLQQVMAIFSEWSNAASFEKLEVDAERGVITEEWRAHQDAKWRTSQARRPF
EECCCCCHHHHHHHHHHHHHHCCCCCCHHEEECHHCCCCCHHHHCCCCCCHHHHHCCCCE
LLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQPNNMTFIVVGDIDSKEALALIKDN
EEECCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHH
LSKLPANKAAENRVWPTKAENHLRFNIINDKENRVNGIALYYRLPMVQVSDEQSFIEQAE
HHHCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCEEEEEEECEEEECCHHHHHHHHH
WSMLVQLFNQRLQERIQSGELKTISGGTARSVKIAPDYQSLFFRVNARGDNMQDAANALM
HHHHHHHHHHHHHHHHHCCCEEEECCCCCCEEEECCCHHHEEEEEECCCCCHHHHHHHHH
AELATIDQHGFSAEELDDVKSTRLTWLKNAVDQQAERDLRMLTSRLASSSLNNTPFLSPE
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCH
ETYQLSKRLWQQITVQSLAEKWQQLRKNQDAFWEQMVNNELAAKKALSPAAILALEKEYA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHEEHHHHC
NKKLAAYIFPGRNLSLTVDADPQAEISSKETLAENLTSLTLSNGARVILAKSAGEEQKLQ
CCEEEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHEEECCCCEEEEEECCCCCCEEE
IIAVSNKGDLSFPAQQKSLIALANKAVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNT
EEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCEEEHHHHCCHHH
LLSVSARTNNPEPGFQLINQRITHSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYE
HEEEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHCHHHHHHHHHHHCCHHHHHHHHHHH
TRYADDRTKLLQENQIAQFTAADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLG
HHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHH
SIKHSDSPLAAGKPLTRATDNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFN
HCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHH
VALAKDLRVNIREQASGAYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVK
HHHHHHHHHHHHHHCCCCEECCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
RLAKGISEQELNEYQQNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMT
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
VENVNTAVKQYLSHPVNTYTGVLLPK
HHHHHHHHHHHHCCCCHHHCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Zn [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Endopeptidases of unknown catalytic mechanism [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]