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The map label for this gene is narU [H]

Identifier: 157160945

GI number: 157160945

Start: 1559484

End: 1560872

Strand: Reverse

Name: narU [H]

Synonym: EcHS_A1553

Alternate gene names: 157160945

Gene position: 1560872-1559484 (Counterclockwise)

Preceding gene: 157160946

Following gene: 157160944

Centisome position: 33.61

GC content: 49.03

Gene sequence:

>1389_bases
ATGGCACTGCAAAATGAGAAAAATAGTCGTTATCTTTTACGCGACTGGAAACCAGAAAATCCGGCCTTCTGGGAAAATAA
AGGAAAACATATTGCTCGAAGAAACCTCTGGATATCAGTCAGTTGTCTACTTCTTGCCTTCTGTGTCTGGATGCTATTTA
GCGCAGTTACCGTTAATCTCAATAAAATCGGTTTTAATTTTACTACCGATCAACTCTTTTTATTAACCGCATTACCCTCC
GTTTCTGGCGCATTATTGCGTGTTCCCTACTCCTTTATGGTGCCTATATTCGGTGGACGCCGATGGACGGTTTTTAGTAC
TGCAATCCTGATTATTCCTTGCGTCTGGCTCGGAATTGCCGTGCAAAATCCGAATACTCCTTTTGGGATATTTATCGTTA
TCGCTTTGCTATGCGGTTTTGCAGGTGCAAACTTTGCTTCGAGCATGGGCAATATCAGTTTCTTCTTTCCAAAAGCCAAA
CAAGGGAGCGCTCTTGGGATTAATGGCGGATTAGGAAACTTAGGTGTAAGTGTAATGCAGCTGGTTGCACCGCTGGTCAT
TTTTGTACCTGTATTTGCCTTTCTCGGCGTCAATGGCGTACCGCAGGCCGACGGTTCGGTGATGTCGCTGGCGAATGCCG
CATGGATTTGGGTACCGCTACTGGCGATTGCCACGATCGCCGCATGGTCAGGGATGAATGATATCGCCAGTTCACGCGCC
TCAATTGCCGACCAGCTCCCTGTCTTACAACGCCTGCATCTCTGGCTGCTGAGCCTGCTTTACCTTGCCACCTTCGGTTC
GTTTATCGGTTTTTCTGCGGGTTTTGCCATGCTGGCAAAAACCCAGTTCCCGGATGTGAATATTCTGCGCCTGGCGTTCT
TTGGCCCATTTATCGGTGCCATCGCGCGGTCGGTTGGTGGTGCTATTTCCGATAAGTTCGGCGGCGTGCGGGTGACGTTG
ATCAACTTTATTTTTATGGCGATTTTCAGTGCCCTGCTGTTCCTTACCTTACCGGGCACAGGCTCCGGTAATTTCATCGC
CTTTTACGCCGTATTTATGGGGCTGTTTCTGACTGCGGGTCTGGGAAGTGGTTCTACTTTCCAGATGATCGCCGTCATCT
TTCGCCAGATAACCATTTATCGGGTAAAGATGAAAGGCGGTAGTGATGAGCAAGCTCAGAAAGAAGCCGTCACCGAAACG
GCGGCGGCTCTGGGCTTTATCTCAGCCATTGGCGCAGTGGGCGGCTTTTTTATTCCGCAGGCGTTTGGCATGTCGCTCAA
TATGACCGGCTCTCCGGTCGGCGCGATGAAAGTGTTTTTAATCTTCTACATCGTTTGTGTGCTGCTGACCTGGCTGGTTT
ATGGTCGGCGGAAGTTCAGCCAAAAATAA

Upstream 100 bases:

>100_bases
GCACTTCACCCAATTCATCCTTAATTCTAATAACTGGCTATTGCGCCTTACCTCCTGTTTTGTCTTTTTTCATTACCAAT
GTGTGCATGTGAGGAACAAT

Downstream 100 bases:

>100_bases
GAGATCGATGCCTGATGTACAACCGTGTCGCATCCGACAAGGCATCAGATCCCAATTCATTCCTGGAGCAGGAGTCATGT
CATGAGTAAACTTTTGGATC

Product: nitrite extrusion protein 2

Products: Proton [Cytoplasm]; nitrite [Periplasm] [C]

Alternate protein names: Nitrite facilitator 2 [H]

Number of amino acids: Translated: 462; Mature: 461

Protein sequence:

>462_residues
MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLLLAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPS
VSGALLRVPYSFMVPIFGGRRWTVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWVPLLAIATIAAWSGMNDIASSRA
SIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFAMLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTL
INFIFMAIFSALLFLTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQAQKEAVTET
AAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLTWLVYGRRKFSQK

Sequences:

>Translated_462_residues
MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLLLAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPS
VSGALLRVPYSFMVPIFGGRRWTVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWVPLLAIATIAAWSGMNDIASSRA
SIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFAMLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTL
INFIFMAIFSALLFLTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQAQKEAVTET
AAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLTWLVYGRRKFSQK
>Mature_461_residues
ALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLLLAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSV
SGALLRVPYSFMVPIFGGRRWTVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAKQ
GSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWVPLLAIATIAAWSGMNDIASSRAS
IADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFAMLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLI
NFIFMAIFSALLFLTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQAQKEAVTETA
AALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLTWLVYGRRKFSQK

Specific function: Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787743, Length=462, Percent_Identity=99.7835497835498, Blast_Score=911, Evalue=0.0,
Organism=Escherichia coli, GI1787475, Length=454, Percent_Identity=75.7709251101322, Blast_Score=677, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004737 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 49882; Mature: 49751

Theoretical pI: Translated: 10.58; Mature: 10.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLLLAFCVWMLFSAVTVNL
CCCCCCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHH
NKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRWTVFSTAILIIPCVWLGIA
HHCCCCCCCHHHHHHHHCCCCCCHHHHCCHHHHHHCCCCCEEHHHHHHHHHHHHHHHHEE
VQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAKQGSALGINGGLGNLGVSVMQ
ECCCCCCHHHHHHHHHHHCCCCCHHHHHCCCEEEEECCCCCCCEEEECCCCHHHHHHHHH
LVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWVPLLAIATIAAWSGMNDIASSRA
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
SIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFAMLAKTQFPDVNILRLAFFGPFIGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
IARSVGGAISDKFGGVRVTLINFIFMAIFSALLFLTLPGTGSGNFIAFYAVFMGLFLTAG
HHHHHCCHHHHCCCCCHHHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHC
LGSGSTFQMIAVIFRQITIYRVKMKGGSDEQAQKEAVTETAAALGFISAIGAVGGFFIPQ
CCCCCHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFGMSLNMTGSPVGAMKVFLIFYIVCVLLTWLVYGRRKFSQK
HHCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLLLAFCVWMLFSAVTVNL
CCCCCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHH
NKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRWTVFSTAILIIPCVWLGIA
HHCCCCCCCHHHHHHHHCCCCCCHHHHCCHHHHHHCCCCCEEHHHHHHHHHHHHHHHHEE
VQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAKQGSALGINGGLGNLGVSVMQ
ECCCCCCHHHHHHHHHHHCCCCCHHHHHCCCEEEEECCCCCCCEEEECCCCHHHHHHHHH
LVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWVPLLAIATIAAWSGMNDIASSRA
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
SIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFAMLAKTQFPDVNILRLAFFGPFIGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
IARSVGGAISDKFGGVRVTLINFIFMAIFSALLFLTLPGTGSGNFIAFYAVFMGLFLTAG
HHHHHCCHHHHCCCCCHHHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHC
LGSGSTFQMIAVIFRQITIYRVKMKGGSDEQAQKEAVTETAAALGFISAIGAVGGFFIPQ
CCCCCHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFGMSLNMTGSPVGAMKVFLIFYIVCVLLTWLVYGRRKFSQK
HHCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: nitrite [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: nitrite [Cytoplasm] + Proton [Periplasm] = Proton [Cytoplasm] + nitrite [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 2233673; 7747940 [H]