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The map label for this gene is ansP

Identifier: 157160933

GI number: 157160933

Start: 1545453

End: 1546952

Strand: Reverse

Name: ansP

Synonym: EcHS_A1540

Alternate gene names: 157160933

Gene position: 1546952-1545453 (Counterclockwise)

Preceding gene: 157160938

Following gene: 157160930

Centisome position: 33.31

GC content: 52.0

Gene sequence:

>1500_bases
ATGAGTAAACACGACACCGACACTTCAGATCAACACGCCGCGAAACGCCGCTGGCTTAATGCCCACGAAGAGGGGTATCA
CAAAGCGATGGGCAATCGCCAGGTACAGATGATCGCCATTGGCGGCGCGATTGGCACCGGCTTGTTTTTAGGTGCAGGAG
CCCGACTGCAAATGGCGGGTCCAGCATTGGCACTGGTTTATTTAATTTGTGGCTTGTTTTCGTTTTTTATTCTGCGTGCA
TTGGGTGAGCTGGTGCTACACCGCCCTTCCAGTGGCAGTTTTGTCTCTTATGCCCGTGAGTTTTTGGGTGAGAAAGCCGC
TTATGTTGCTGGCTGGATGTACTTCATCAACTGGGCGATGACGGGGATTGTTGATATTACCGCCGTCGCTCTGTATATGC
ATTACTGGGGTGCGTTTGGCGGCGTGCCGCAGTGGGTCTTTGCGCTCGCTGCACTTACCATCGTTGGCACCATGAATATG
ATCGGTGTGAAATGGTTTGCGGAGATGGAGTTCTGGTTTGCGCTTATTAAAGTGCTCGCCATTGTGACCTTTTTGGTCGT
GGGTACAGTGTTCCTCGGTAGTGGTCAGCCGCTGGATGGCAACACCACTGGCTTTCATTTAATTACCGATAATGGCGGCT
TCTTCCCCCACGGTTTGCTGCCTGCTCTGGTGTTGATTCAGGGCGTAGTGTTTGCTTTTGCCTCCATTGAAATGGTGGGT
ACAGCTGCCGGAGAATGTAAAGATCCGCAGACCATGGTGCCTAAAGCGATTAACAGCGTGATTTGGCGTATTGGCCTGTT
TTACGTCGGCTCCGTGGTGTTGCTGGTTATGCTATTGCCGTGGAGCGCGTATCAGGCGGGGCAAAGTCCGTTCGTGACGT
TTTTCTCTAAACTGGGTGTGCCATATATCGGCAGCATTATGAACATTGTGGTGCTGACCGCTGCCCTCTCCAGCCTGAAC
TCAGGTCTGTACTGCACCGGACGTATTCTGCGCTCAATGGCGATGGGCGGTTCCGCACCGAGTTTTATGGCGAAAATGAG
TCGTCAGCATGTGCCGTATGCCGGGATTCTAGCGACACTAGTTGTGTATGTCGTCGGCGTATTCCTCAACTATCTGGTGC
CGTCGCGCGTATTTGAGATTGTGTTGAACTTCGCGTCGCTGGGAATCATCGCTTCATGGGCGTTTATCATCGTGTGCCAG
ATGCGCCTGCGTAAAGCGATTAAAGAAGGCAAAGCAGCGGATGTCAGTTTTAAACTGCCTGGCGCGCCCTTCACTTCCTG
GCTGACATTACTGTTTTTACTGAGTGTCCTTGTGCTGATGGCGTTCGATTACCCGAACGGGACTTATACTATCGCGGCGC
TGCCGATTATCGGTATTCTGCTGGTTATAGGCTGGTTTGGTGTGCGCAAACGCGTTGCTGAAATTCACAGCACTGCGCCA
GTCGTCGAGGAAGATGAAGAAAAACAGGAAATCGTGTTTAAGCCTGAAACGGCGAGCTAA

Upstream 100 bases:

>100_bases
ACCCATAAAGAATAATGGTGATAACTATCATCGCCAGGATGAATAAACATTGTTCATGGCAACTTATATGACTTTTTCAT
TAAAGCAATCAGGGAGAGCA

Downstream 100 bases:

>100_bases
TTGCAAAATTGCCCGGCTACGTGCCGGGCAACTTCAAATTACTGACGAAGGTGTGCCGCATGCGGCGTGATAAAAGTCAG
GCAAGTCTCTCCGTTTTCAC

Product: L-asparagine permease

Products: Proton [Cytoplasm]; L-asparagine [Cytoplasm] [C]

Alternate protein names: L-asparagine transport protein

Number of amino acids: Translated: 499; Mature: 498

Protein sequence:

>499_residues
MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAIGTGLFLGAGARLQMAGPALALVYLICGLFSFFILRA
LGELVLHRPSSGSFVSYAREFLGEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHGLLPALVLIQGVVFAFASIEMVG
TAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLVMLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLN
SGLYCTGRILRSMAMGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIASWAFIIVCQ
MRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYTIAALPIIGILLVIGWFGVRKRVAEIHSTAP
VVEEDEEKQEIVFKPETAS

Sequences:

>Translated_499_residues
MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAIGTGLFLGAGARLQMAGPALALVYLICGLFSFFILRA
LGELVLHRPSSGSFVSYAREFLGEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHGLLPALVLIQGVVFAFASIEMVG
TAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLVMLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLN
SGLYCTGRILRSMAMGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIASWAFIIVCQ
MRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYTIAALPIIGILLVIGWFGVRKRVAEIHSTAP
VVEEDEEKQEIVFKPETAS
>Mature_498_residues
SKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAIGTGLFLGAGARLQMAGPALALVYLICGLFSFFILRAL
GELVLHRPSSGSFVSYAREFLGEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNMI
GVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHGLLPALVLIQGVVFAFASIEMVGT
AAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLVMLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNS
GLYCTGRILRSMAMGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIASWAFIIVCQM
RLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYTIAALPIIGILLVIGWFGVRKRVAEIHSTAPV
VEEDEEKQEIVFKPETAS

Specific function: Unknown

COG id: COG1113

COG function: function code E; Gamma-aminobutyrate permease and related permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily. Amino acid transporter (AAT) (TC 2.A.3.1) family

Homologues:

Organism=Homo sapiens, GI4507047, Length=430, Percent_Identity=23.0232558139535, Blast_Score=80, Evalue=5e-15,
Organism=Homo sapiens, GI258645169, Length=415, Percent_Identity=21.6867469879518, Blast_Score=74, Evalue=4e-13,
Organism=Escherichia coli, GI87081915, Length=499, Percent_Identity=100, Blast_Score=1007, Evalue=0.0,
Organism=Escherichia coli, GI1786302, Length=456, Percent_Identity=36.8421052631579, Blast_Score=317, Evalue=1e-87,
Organism=Escherichia coli, GI1786789, Length=439, Percent_Identity=36.6742596810934, Blast_Score=309, Evalue=3e-85,
Organism=Escherichia coli, GI1790653, Length=459, Percent_Identity=37.2549019607843, Blast_Score=306, Evalue=2e-84,
Organism=Escherichia coli, GI1786602, Length=449, Percent_Identity=36.7483296213808, Blast_Score=298, Evalue=4e-82,
Organism=Escherichia coli, GI48994972, Length=421, Percent_Identity=38.7173396674584, Blast_Score=295, Evalue=6e-81,
Organism=Escherichia coli, GI87081708, Length=458, Percent_Identity=33.1877729257642, Blast_Score=250, Evalue=1e-67,
Organism=Escherichia coli, GI1789017, Length=423, Percent_Identity=33.5697399527187, Blast_Score=245, Evalue=6e-66,
Organism=Escherichia coli, GI1788480, Length=470, Percent_Identity=32.3404255319149, Blast_Score=228, Evalue=6e-61,
Organism=Caenorhabditis elegans, GI17533459, Length=436, Percent_Identity=22.4770642201835, Blast_Score=69, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17532491, Length=437, Percent_Identity=21.7391304347826, Blast_Score=69, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6324061, Length=464, Percent_Identity=29.0948275862069, Blast_Score=204, Evalue=3e-53,
Organism=Saccharomyces cerevisiae, GI6320772, Length=438, Percent_Identity=29.4520547945205, Blast_Score=190, Evalue=6e-49,
Organism=Saccharomyces cerevisiae, GI6322892, Length=456, Percent_Identity=32.4561403508772, Blast_Score=187, Evalue=3e-48,
Organism=Saccharomyces cerevisiae, GI6324990, Length=522, Percent_Identity=26.4367816091954, Blast_Score=180, Evalue=4e-46,
Organism=Saccharomyces cerevisiae, GI6324924, Length=513, Percent_Identity=28.4600389863548, Blast_Score=177, Evalue=3e-45,
Organism=Saccharomyces cerevisiae, GI6321629, Length=429, Percent_Identity=28.9044289044289, Blast_Score=159, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6321053, Length=461, Percent_Identity=27.3318872017354, Blast_Score=157, Evalue=4e-39,
Organism=Saccharomyces cerevisiae, GI6324059, Length=465, Percent_Identity=28.6021505376344, Blast_Score=157, Evalue=5e-39,
Organism=Saccharomyces cerevisiae, GI6324553, Length=399, Percent_Identity=30.5764411027569, Blast_Score=156, Evalue=6e-39,
Organism=Saccharomyces cerevisiae, GI6322967, Length=534, Percent_Identity=26.7790262172285, Blast_Score=152, Evalue=1e-37,
Organism=Saccharomyces cerevisiae, GI6320717, Length=416, Percent_Identity=28.125, Blast_Score=149, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6324981, Length=423, Percent_Identity=27.6595744680851, Blast_Score=144, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6320251, Length=458, Percent_Identity=26.6375545851528, Blast_Score=142, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6319543, Length=424, Percent_Identity=27.3584905660377, Blast_Score=140, Evalue=5e-34,
Organism=Saccharomyces cerevisiae, GI6319824, Length=414, Percent_Identity=26.8115942028986, Blast_Score=140, Evalue=5e-34,
Organism=Saccharomyces cerevisiae, GI6319608, Length=460, Percent_Identity=26.0869565217391, Blast_Score=138, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6319542, Length=410, Percent_Identity=26.0975609756098, Blast_Score=128, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6320364, Length=503, Percent_Identity=21.0735586481113, Blast_Score=70, Evalue=6e-13,
Organism=Drosophila melanogaster, GI221512776, Length=424, Percent_Identity=21.2264150943396, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24666159, Length=424, Percent_Identity=21.2264150943396, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24668806, Length=428, Percent_Identity=22.4299065420561, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI21356285, Length=428, Percent_Identity=22.4299065420561, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24668802, Length=428, Percent_Identity=22.4299065420561, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ANSP_ECOLI (P77610)

Other databases:

- EMBL:   U00096
- EMBL:   AP009048
- RefSeq:   AP_002076.1
- RefSeq:   NP_415970.4
- ProteinModelPortal:   P77610
- STRING:   P77610
- EnsemblBacteria:   EBESCT00000002459
- EnsemblBacteria:   EBESCT00000002460
- EnsemblBacteria:   EBESCT00000016851
- GeneID:   946019
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW5234
- KEGG:   eco:b1453
- EchoBASE:   EB3538
- EcoGene:   EG13776
- eggNOG:   COG1113
- GeneTree:   EBGT00050000009005
- HOGENOM:   HBG492579
- OMA:   FIVVCQM
- ProtClustDB:   PRK15049
- BioCyc:   EcoCyc:ANSP-MONOMER
- Genevestigator:   P77610
- InterPro:   IPR004841
- InterPro:   IPR002293
- InterPro:   IPR004840
- PANTHER:   PTHR11785
- PIRSF:   PIRSF006060

Pfam domain/function: PF00324 AA_permease

EC number: NA

Molecular weight: Translated: 54234; Mature: 54103

Theoretical pI: Translated: 8.97; Mature: 8.97

Prosite motif: PS00218 AMINO_ACID_PERMEASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x14787a7c)-; HASH(0x13ea4108)-; HASH(0x1247ad20)-; HASH(0x1463ae1c)-; HASH(0x14828da0)-; HASH(0x1472ed24)-; HASH(0x13222578)-; HASH(0x13e0e6dc)-; HASH(0x11a39140)-; HASH(0x14ac9410)-; HASH(0x1484aea8)-; HASH(0x1356ac04)-;

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAIGTGLFLGAGARLQMAG
CCCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCEEEEEEECCHHHHHHHHCCCCCEEECC
PALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFLGEKAAYVAGWMYFINWAM
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHH
TGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNMIGVKWFAEMEFWFALIKVLA
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHGLLPALVLIQGVVFAFASIEMVG
HHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
TAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLVMLLPWSAYQAGQSPFVTFFSKLGV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHCCC
PYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMAMGGSAPSFMAKMSRQHVPYAGILATL
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHH
VVYVVGVFLNYLVPSRVFEIVLNFASLGIIASWAFIIVCQMRLRKAIKEGKAADVSFKLP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECC
GAPFTSWLTLLFLLSVLVLMAFDYPNGTYTIAALPIIGILLVIGWFGVRKRVAEIHSTAP
CCCHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VVEEDEEKQEIVFKPETAS
CCCCCCCCCCEEECCCCCH
>Mature Secondary Structure 
SKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAIGTGLFLGAGARLQMAG
CCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCEEEEEEECCHHHHHHHHCCCCCEEECC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CCCCCCCCCCEEECCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-asparagine [Periplasm]; Proton [Periplasm] [C]

Specific reaction: L-asparagine [Periplasm] + Proton [Periplasm] = Proton [Cytoplasm] + L-asparagine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503