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The map label for this gene is mppA

Identifier: 157160841

GI number: 157160841

Start: 1440678

End: 1442291

Strand: Direct

Name: mppA

Synonym: EcHS_A1446

Alternate gene names: 157160841

Gene position: 1440678-1442291 (Clockwise)

Preceding gene: 157160839

Following gene: 157160843

Centisome position: 31.03

GC content: 47.96

Gene sequence:

>1614_bases
ATGAAGCACTCTGTTTCAGTCACGTGTTGTGCGCTGTTGGTCAGCAGCATTTCTCTTTCGTATGCTGCAGAAGTTCCGAG
CGGCACAGTACTGGCAGAGAAGCAGGAGCTGGTGCGCCACATTAAAGATGAGCCTGCGTCGCTGGATCCCGCTAAAGCCG
TGGGCCTGCCAGAGATTCAGGTCATTCGCGATCTGTTTGAAGGTCTGGTGAATCAGAACGAAAAAGGGGAGATTGTCCCC
GGCGTTGCGACTCAGTGGAAAAGTAATGACAACCGTATCTGGACTTTTACCCTGCGCGATAACGCAAAATGGGCGGATGG
CACACCGGTAACGGCGCAAGATTTTGTCTACAGCTGGCAACGTCTGGTGGACCCAAAAACATTGTCGCCATTTGCATGGT
TTGCCGCGCTGGCGGGAATCAACAACGCACAGGCGATTATTGATGGTAAAGCTACGCCTGACCAGCTTGGCGTCACCGCA
GTTGATGCCCATACTTTGAAAATTCAGCTTGATAAACCGTTGCCGTGGTTTGTGAATTTAACCGCTAACTTTGCCTTCTT
CCCGGTGCAAAAAGCCAACGTAGAAAGCGGTAAAGAGTGGACGAAACCCGGAAATCTGATCGGCAATGGCGCTTATGTTC
TTAAAGAGCGCGTAGTCAATGAAAAACTGGTCGTGGTACCGAATACCCATTATTGGGATAACGCCAAAACGGTACTGCAA
AAAGTGACCTTCCTGCCAATTAATCAGGAATCCGCAGCCACTAAGCGTTACCTCGCGGGGGATATTGATATCACCGAATC
CTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATT
ATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCGCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGC
CTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTACGCC
GGAACCTTCGCCGTTTGAACAAATGAGTCAGGAAGAACTGAATGCGCAGGCAAAAACTTTGTTGAGCGCAGCTGGTTATG
GTCCGCAAAAACCGCTGAAGCTGACGCTTTTGTATAACACTTCAGAAAACCATCAAAAAATTGCGATTGCTGTAGCATCG
ATGTGGAAAAAGAACCTTGGCGTAGATGTAAAATTGCAAAATCAGGAATGGAAAACCTATATCGATAGCCGTAACACCGG
CAATTTTGATGTTATCCGCGCCTCGTGGGTGGGGGATTATAATGAACCCTCCACTTTCCTGACATTATTAACGTCAACGC
ATTCAGGAAATATTTCACGCTTTAACAATCCGGCATATGACAAAGTTCTGGCCCAGGCATCGACGGAAAATACCGTTAAA
GCGCGTAATGCCGATTACAACGCGGCAGAAAAAATCCTCATGGAGCAAGCACCGATTGCACCAATTTATCAATATACCAA
TGGACGATTAATCAAGCCGTGGCTGAAAGGTTATCCCATTAATAATCCTGAAGATGTGGCGTACAGTCGGACTATGTATA
TTGTGAAGCATTGA

Upstream 100 bases:

>100_bases
GATGCTTCCTTTGTCACTTTTTTGATGGAAGTTGTTTGCATTTCTTTAAGGCGAAACAAATAATTACGCATCAATTTTAA
TGTCGGTTAGAGGGAAACTT

Downstream 100 bases:

>100_bases
TGTGATGGGAACTGGCGTTACCCTTGTGCATAACGCCAGTGATGTCTGATTTAGCGACCCTGTTCCGGCGGTGTAATGTT
ATCCATATACAGCGTCTGGC

Product: periplasmic murein peptide-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 537; Mature: 537

Protein sequence:

>537_residues
MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDEPASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVP
GVATQWKSNDNRIWTFTLRDNAKWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERVVNEKLVVVPNTHYWDNAKTVLQ
KVTFLPINQESAATKRYLAGDIDITESFPKNMYQKLLKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRR
LMTEKVLGTGEKPAWHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQKIAIAVAS
MWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLLTSTHSGNISRFNNPAYDKVLAQASTENTVK
ARNADYNAAEKILMEQAPIAPIYQYTNGRLIKPWLKGYPINNPEDVAYSRTMYIVKH

Sequences:

>Translated_537_residues
MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDEPASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVP
GVATQWKSNDNRIWTFTLRDNAKWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERVVNEKLVVVPNTHYWDNAKTVLQ
KVTFLPINQESAATKRYLAGDIDITESFPKNMYQKLLKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRR
LMTEKVLGTGEKPAWHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQKIAIAVAS
MWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLLTSTHSGNISRFNNPAYDKVLAQASTENTVK
ARNADYNAAEKILMEQAPIAPIYQYTNGRLIKPWLKGYPINNPEDVAYSRTMYIVKH
>Mature_537_residues
MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDEPASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVP
GVATQWKSNDNRIWTFTLRDNAKWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERVVNEKLVVVPNTHYWDNAKTVLQ
KVTFLPINQESAATKRYLAGDIDITESFPKNMYQKLLKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRR
LMTEKVLGTGEKPAWHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQKIAIAVAS
MWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLLTSTHSGNISRFNNPAYDKVLAQASTENTVK
ARNADYNAAEKILMEQAPIAPIYQYTNGRLIKPWLKGYPINNPEDVAYSRTMYIVKH

Specific function: Essential for the uptake of the murein peptide L-alanyl- gamma-D-glutamyl-meso-diaminopimelate. Also transports some alpha- linked peptides such as Pro-Phe-Lys with low affinity. The transport is effected by the oligopeptide permease system

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family

Homologues:

Organism=Escherichia coli, GI87081878, Length=537, Percent_Identity=100, Blast_Score=1109, Evalue=0.0,
Organism=Escherichia coli, GI1787495, Length=531, Percent_Identity=48.210922787194, Blast_Score=508, Evalue=1e-145,
Organism=Escherichia coli, GI1789397, Length=529, Percent_Identity=42.34404536862, Blast_Score=404, Evalue=1e-114,
Organism=Escherichia coli, GI1787762, Length=538, Percent_Identity=28.2527881040892, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI1789887, Length=473, Percent_Identity=25.1585623678647, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI1789966, Length=466, Percent_Identity=21.8884120171674, Blast_Score=97, Evalue=4e-21,
Organism=Escherichia coli, GI1787052, Length=503, Percent_Identity=20.8747514910537, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MPPA_ECOLI (P77348)

Other databases:

- EMBL:   U88242
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   D64882
- RefSeq:   AP_001957.1
- RefSeq:   NP_415845.2
- ProteinModelPortal:   P77348
- SMR:   P77348
- STRING:   P77348
- SWISS-2DPAGE:   P77348
- EnsemblBacteria:   EBESCT00000004603
- EnsemblBacteria:   EBESCT00000016737
- GeneID:   945951
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW1322
- KEGG:   eco:b1329
- EchoBASE:   EB3158
- EcoGene:   EG13376
- eggNOG:   COG4166
- GeneTree:   EBGT00070000031725
- HOGENOM:   HBG688042
- OMA:   RDDAQWA
- ProtClustDB:   CLSK880102
- BioCyc:   EcoCyc:G6665-MONOMER
- Genevestigator:   P77348
- InterPro:   IPR000914

Pfam domain/function: PF00496 SBP_bac_5

EC number: NA

Molecular weight: Translated: 59901; Mature: 59901

Theoretical pI: Translated: 8.74; Mature: 8.74

Prosite motif: PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDEPASLDPAKAVGLPEIQ
CCCCCHHHHHHHHHHHHHHHEEECCCCCCEEHHHHHHHHHHCCCCCCCCHHHHCCCCHHH
VIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNAKWADGTPVTAQDFVYSWQ
HHHHHHHHHHCCCCCCCCCCCCHHCCCCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHH
RLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTAVDAHTLKIQLDKPLPWFVNL
HHCCCCCCCHHHHHHHHHCCCCCEEEEECCCCHHHCCEEEEEEEEEEEEECCCCCEEEEE
TANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERVVNEKLVVVPNTHYWDNAKTVLQ
EECEEEEEEECCCCCCCCCCCCCCCEECCCHHHHHHHHCCCEEEEECCCCCCCCHHHHHH
KVTFLPINQESAATKRYLAGDIDITESFPKNMYQKLLKDIPGQVYTPPQLGTYYYAFNTQ
HHHEEECCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEECCCCCCEEEEEEECC
KGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPAWHFTPDVTAGFTPEPSPFEQMSQEEL
CCCCHHHEEEEEEEEHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHH
NAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQKIAIAVASMWKKNLGVDVKLQNQEWKTY
HHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCEEEEEEHHHHHHCCCEEEEECCCCHHHH
IDSRNTGNFDVIRASWVGDYNEPSTFLTLLTSTHSGNISRFNNPAYDKVLAQASTENTVK
CCCCCCCCEEEEEEEECCCCCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHCCCCCCEE
ARNADYNAAEKILMEQAPIAPIYQYTNGRLIKPWLKGYPINNPEDVAYSRTMYIVKH
ECCCCCHHHHHHHHHCCCCCCEEEECCCEEECHHHCCCCCCCCCCCEEEEEEEEEEC
>Mature Secondary Structure
MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDEPASLDPAKAVGLPEIQ
CCCCCHHHHHHHHHHHHHHHEEECCCCCCEEHHHHHHHHHHCCCCCCCCHHHHCCCCHHH
VIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNAKWADGTPVTAQDFVYSWQ
HHHHHHHHHHCCCCCCCCCCCCHHCCCCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHH
RLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTAVDAHTLKIQLDKPLPWFVNL
HHCCCCCCCHHHHHHHHHCCCCCEEEEECCCCHHHCCEEEEEEEEEEEEECCCCCEEEEE
TANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERVVNEKLVVVPNTHYWDNAKTVLQ
EECEEEEEEECCCCCCCCCCCCCCCEECCCHHHHHHHHCCCEEEEECCCCCCCCHHHHHH
KVTFLPINQESAATKRYLAGDIDITESFPKNMYQKLLKDIPGQVYTPPQLGTYYYAFNTQ
HHHEEECCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEECCCCCCEEEEEEECC
KGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPAWHFTPDVTAGFTPEPSPFEQMSQEEL
CCCCHHHEEEEEEEEHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHH
NAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQKIAIAVASMWKKNLGVDVKLQNQEWKTY
HHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCEEEEEEHHHHHHCCCEEEEECCCCHHHH
IDSRNTGNFDVIRASWVGDYNEPSTFLTLLTSTHSGNISRFNNPAYDKVLAQASTENTVK
CCCCCCCCEEEEEEEECCCCCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHCCCCCCEE
ARNADYNAAEKILMEQAPIAPIYQYTNGRLIKPWLKGYPINNPEDVAYSRTMYIVKH
ECCCCCHHHHHHHHHCCCCCCEEEECCCEEECHHHCCCCCCCCCCCEEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9495761; 9097039; 9278503