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The map label for this gene is ycjW

Identifier: 157160831

GI number: 157160831

Start: 1430414

End: 1431412

Strand: Reverse

Name: ycjW

Synonym: EcHS_A1435

Alternate gene names: 157160831

Gene position: 1431412-1430414 (Counterclockwise)

Preceding gene: 157160835

Following gene: 157160814

Centisome position: 30.83

GC content: 45.45

Gene sequence:

>999_bases
ATGTCCCCTACTATTTATGATATTGCCAGGGTTGCAGGCGTATCAAAATCCACCGTATCACGCGTGCTGAATAAGCAAAC
TAATATCTCCCCGGAAGCGCGCGAAAAAGTGTTACGGGCCATTGAAGAATTACAGTATCAGCCAAACAAGCTGGCCCGCG
CGCTGACCTCTTCGGGTTTTGATGCCATTATGGTGATTTCTACCCGTTCGACCAAAACTACGGCGGGTAATCCGTTTTTC
TCTGAAGTTTTGCATGCCATCACCGCCAAAGCTGAAGAAGAAGGTTTCGACGTGATATTACAAACGTCGCACAATCCGGC
AGAAGATCTGCAAAAATGCGAAAGCAAAATTAAGCAGAAAATGATTAAAGGCATTATTATGCTCAGTTCGCCAGCGGATG
AGTCATTTTTTGCCCAACTCGATAAATATGATATTCCGGTGGTGGTGATTGGCAAAGTTGAAGGTCAATATGCCCATGTT
TATTCTGTCGATACCGATAATTTTGGCGACAGCATTGCGTTGACCGATGCATTAATTGAAAGTGGGCATCAAAATATTGC
CTGCCTGCATGCTCCGCTTGATGTCCATGTTTCGGTTGATCGCGTTAATGGTTATAAGCAGAGCCTGGCTGCGCATAATA
TTGCAGTGCGTGATGAATGGATTGTTGATGGCGGTTATACCCATGAAACTGCATTAAAAGCAGCACGGCAATTATTAAGC
CAGTCACCGTTGCCTGAGGCGGTGTTTGCCACTGACAGCCTGAAATTAATGAGCATTTATCGTGCGGCAGCAGAGAAAAA
TATTGCTATTCCGCAGCAGTTAGCGGTGGTGGGTTATAGCAATGAAACGCTGTCATTTATTTTAACGCCTGCACCGGGCG
GCATCGATGTTCCGACGCAGGAGTTAGGTCAACAAAGCTGCGAGTTATTATTCCGCTTAATTTCTGGAAAACCGTCACCA
CAGAATATTACCGTTGCCACGCATATGACGCTGAAATAA

Upstream 100 bases:

>100_bases
TGTGGGGGCCAGGCCATTGATTATCAACTGCCCGTGTTGTCATCGACCGGCGAACTCCATAAGATGTGCAGCATTCACGT
TCGGACGGAGTTCCTGACCT

Downstream 100 bases:

>100_bases
ATACCAGCTACCTTGCCTGAATGCCGGATGCGATGCTAGCGCATCTTATCCGGCCTACATTTATAGATCGGATAAAGTAC
TCGCGCCGCATCCGGCAATT

Product: LacI family transcription regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEELQYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFF
SEVLHAITAKAEEEGFDVILQTSHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRDEWIVDGGYTHETALKAARQLLS
QSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAVVGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSP
QNITVATHMTLK

Sequences:

>Translated_332_residues
MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEELQYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFF
SEVLHAITAKAEEEGFDVILQTSHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRDEWIVDGGYTHETALKAARQLLS
QSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAVVGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSP
QNITVATHMTLK
>Mature_331_residues
SPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEELQYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFS
EVLHAITAKAEEEGFDVILQTSHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHVY
SVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRDEWIVDGGYTHETALKAARQLLSQ
SPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAVVGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQ
NITVATHMTLK

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1787580, Length=332, Percent_Identity=100, Blast_Score=686, Evalue=0.0,
Organism=Escherichia coli, GI1787948, Length=324, Percent_Identity=30.8641975308642, Blast_Score=163, Evalue=1e-41,
Organism=Escherichia coli, GI1790194, Length=319, Percent_Identity=30.4075235109718, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI1789068, Length=323, Percent_Identity=29.1021671826625, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1790369, Length=316, Percent_Identity=27.5316455696203, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1788474, Length=320, Percent_Identity=28.75, Blast_Score=114, Evalue=8e-27,
Organism=Escherichia coli, GI48994940, Length=317, Percent_Identity=25.5520504731861, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1787906, Length=313, Percent_Identity=26.1980830670927, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1789202, Length=320, Percent_Identity=26.5625, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1786540, Length=328, Percent_Identity=25.3048780487805, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1786268, Length=289, Percent_Identity=23.5294117647059, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1790689, Length=327, Percent_Identity=27.82874617737, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1790715, Length=320, Percent_Identity=20.9375, Blast_Score=73, Evalue=3e-14,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): YCJW_ECOLI (P77615)

Other databases:

- EMBL:   U00096
- EMBL:   AP009048
- PIR:   C64881
- RefSeq:   AP_001947.1
- RefSeq:   NP_415836.1
- ProteinModelPortal:   P77615
- SMR:   P77615
- DIP:   DIP-11614N
- IntAct:   P77615
- STRING:   P77615
- EnsemblBacteria:   EBESCT00000003876
- EnsemblBacteria:   EBESCT00000016516
- GeneID:   945875
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW1313
- KEGG:   eco:b1320
- EchoBASE:   EB3679
- EcoGene:   EG13920
- eggNOG:   COG1609
- GeneTree:   EBGT00050000009236
- HOGENOM:   HBG753640
- OMA:   THESALD
- ProtClustDB:   CLSK880108
- BioCyc:   EcoCyc:G6658-MONOMER
- Genevestigator:   P77615
- GO:   GO:0005622
- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761
- PRINTS:   PR00036
- SMART:   SM00354

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1; SSF47413 Lambda_like_DNA

EC number: NA

Molecular weight: Translated: 36135; Mature: 36003

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS00356 HTH_LACI_1; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEELQYQPNKLARALTSSGF
CCCHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCC
DAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQTSHNPAEDLQKCESKIKQK
CEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHH
MIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHVYSVDTDNFGDSIALTDALIE
HHHCEEEECCCCCHHHHHHHHCCCCCEEEEEECCCCEEEEEEECCCCCCCCHHHHHHHHH
SGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRDEWIVDGGYTHETALKAARQLLS
CCCCCEEEEECCCEEEEEEEHHCCHHHHHHHCCEEECCEEEECCCCCHHHHHHHHHHHHH
QSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAVVGYSNETLSFILTPAPGGIDVPTQ
CCCCCCHHHCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCEEEEEEECCCCCCCCCHH
ELGQQSCELLFRLISGKPSPQNITVATHMTLK
HHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEC
>Mature Secondary Structure 
SPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEELQYQPNKLARALTSSGF
CCHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCC
DAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQTSHNPAEDLQKCESKIKQK
CEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHH
MIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHVYSVDTDNFGDSIALTDALIE
HHHCEEEECCCCCHHHHHHHHCCCCCEEEEEECCCCEEEEEEECCCCCCCCHHHHHHHHH
SGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRDEWIVDGGYTHETALKAARQLLS
CCCCCEEEEECCCEEEEEEEHHCCHHHHHHHCCEEECCEEEECCCCCHHHHHHHHHHHHH
QSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAVVGYSNETLSFILTPAPGGIDVPTQ
CCCCCCHHHCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCEEEEEEECCCCCCCCCHH
ELGQQSCELLFRLISGKPSPQNITVATHMTLK
HHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503