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The map label for this gene is rnb [H]

Identifier: 157160795

GI number: 157160795

Start: 1394538

End: 1396472

Strand: Reverse

Name: rnb [H]

Synonym: EcHS_A1399

Alternate gene names: 157160795

Gene position: 1396472-1394538 (Counterclockwise)

Preceding gene: 157160796

Following gene: 157160794

Centisome position: 30.07

GC content: 52.97

Gene sequence:

>1935_bases
ATGTTTCAGGACAACCCGCTGCTAGCGCAGCTTAAACAGCAACTGCATTCCCAGACGCCACGCGCTGAAGGGGTGGTAAA
AGCCACAGAAAAAGGCTTTGGCTTCCTGGAAGTCGACGCGCAAAAAAGTTATTTCATTCCGCCGCCGCAGATGAAAAAAG
TCATGCATGGCGACCGAATTATCGCGGTGATCCACAGTGAAAAAGAACGTGAATCCGCAGAGCCAGAAGAACTGGTTGAA
CCGTTCCTGACTCGTTTCGTGGGTAAGGTTCAGGGCAAAAATGACCGTCTGGCCATCGTTCCTGATCATCCACTCTTAAA
AGACGCCATTCCTTGCCGCGCAGCCCGTGGCCTGAACCACGAGTTTAAAGAAGGCGACTGGGCGGTTGCCGAAATGCGCC
GTCATCCGCTGAAAGGCGATCGTTCTTTCTATGCAGAACTGACACAATACATCACTTTTGGTGACGATCACTTTGTACCG
TGGTGGGTTACCCTTGCACGCCATAATCTGGAAAAAGAAGCACCAGACGGCGTCGCTACCGAAATGCTCGATGAAGGTCT
GGTTCGTGAAGATCTGACCGCGCTGGATTTTGTCACCATCGACAGTGCCAGCACAGAAGATATGGATGACGCCCTTTTCG
CTAAGGCGTTGCCGGATGACAAACTTCAGCTGATTGTGGCGATTGCCGATCCAACCGCGTGGATTGCTGAAGGCAGTAAG
CTGGACAAAGCCGCGAAAATTCGCGCATTCACCAACTATCTGCCTGGCTTCAACATCCCTATGCTGCCTCGCGAGCTTTC
TGACGATCTCTGCTCACTGCGCGCCAATGAAGTCCGCCCGGTACTGGCATGCCGCATGACGCTCTCCGCTGATGGCACCA
TTGAAGATAATATCGAATTCTTTGCCGCCACCATCGAATCCAAAGCGAAGCTGGTGTATGACCAGGTTTCTGACTGGCTG
GAAAATACCGGTGACTGGAAGCCTGAAAGTGAAGCAATTGCCGAACAAGTCCGTTTGTTAGCGCAAATTTGCCAACGCCG
CGGCGAGTGGCGTCATAACCACGCACTGGTGTTTAAAGATCGCCCGGATTACCGCTTTATTCTCGGTGAAAAAGGTGAAG
TGCTGGATATCGTCGCCGAGCCTCGTCGCATTGCCAACCGTATCGTCGAAGAAGCGATGATTGCCGCTAACATTTGTGCG
GCCCGCGTACTGCGCGATAAGCTCGGTTTTGGCATCTATAACGTGCATATGGGCTTTGATCCGGCGAATGCCGACGCGCT
GGCAGCGTTGCTGAAAACGCACGGTCTGCATGTCGATGCCGAAGAAGTGCTCACGCTGGACGGTTTCTGCAAACTGCGTC
GTGAACTGGATGCGCAACCAACTGGTTTCCTCGACAGCCGCATTCGTCGCTTCCAGTCATTTGCTGAAATTAGCACTGAA
CCCGGTCCTCACTTTGGCCTCGGTCTGGAAGCATACGCCACCTGGACATCGCCGATCCGTAAATATGGCGACATGATCAA
CCACCGTCTGCTGAAAGCGGTTATCAAAGGCGAAACTGCGACGCGTCCACAGGATGAGATCACTGTCCAAATGGCCGAGC
GTCGCCGTCTCAATCGGATGGCAGAACGTGATGTTGGTGACTGGTTATACGCACGCTTCCTGAAAGACAAAGCCGGGACC
GACACCCGTTTCGCGGCGGAAATTGTCGATATCAGCCGTGGCGGCATGCGTGTTCGTTTGGTTGATAACGGCGCTATCGC
CTTTATTCCTGCACCTTTCTTACACGCTGTGCGCGATGAAATGGTTTGCAGCCAGGAAAACGGCACCGTACAAATTAAAG
GTGAAACGGTTTATAAAGTAACTGACGTTATTGACGTCACCATTGCCGAAGTCCGCATGGAAACCCGCAGCATTATTGCG
CGCCCGGTCGCGTAA

Upstream 100 bases:

>100_bases
CGCTTAAAAATCGCGTTGGGTGAGACATATTAACCTTGCCGCGTCAGACAGATTCGCGTAAAACTGTCAGCCGCTCTAAT
GGCCACCAAAATAGACAATT

Downstream 100 bases:

>100_bases
TCTCCTTTCACGGCCCATTCCTTATGAATGGGCCGTTTATTTCCCCGCTCTACCTTCATCATATCCCGGCAGTTTTTTAA
CTGACTTTCGTTTGAAAACT

Product: exoribonuclease II

Products: NA

Alternate protein names: Exoribonuclease II; RNase II; Ribonuclease II [H]

Number of amino acids: Translated: 644; Mature: 644

Protein sequence:

>644_residues
MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSYFIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVE
PFLTRFVGKVQGKNDRLAIVPDHPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALPDDKLQLIVAIADPTAWIAEGSK
LDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRANEVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWL
ENTGDWKPESEAIAEQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEAMIAANICA
ARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFCKLRRELDAQPTGFLDSRIRRFQSFAEISTE
PGPHFGLGLEAYATWTSPIRKYGDMINHRLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGT
DTRFAAEIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDEMVCSQENGTVQIKGETVYKVTDVIDVTIAEVRMETRSIIA
RPVA

Sequences:

>Translated_644_residues
MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSYFIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVE
PFLTRFVGKVQGKNDRLAIVPDHPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALPDDKLQLIVAIADPTAWIAEGSK
LDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRANEVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWL
ENTGDWKPESEAIAEQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEAMIAANICA
ARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFCKLRRELDAQPTGFLDSRIRRFQSFAEISTE
PGPHFGLGLEAYATWTSPIRKYGDMINHRLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGT
DTRFAAEIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDEMVCSQENGTVQIKGETVYKVTDVIDVTIAEVRMETRSIIA
RPVA
>Mature_644_residues
MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSYFIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVE
PFLTRFVGKVQGKNDRLAIVPDHPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALPDDKLQLIVAIADPTAWIAEGSK
LDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRANEVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWL
ENTGDWKPESEAIAEQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEAMIAANICA
ARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFCKLRRELDAQPTGFLDSRIRRFQSFAEISTE
PGPHFGLGLEAYATWTSPIRKYGDMINHRLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGT
DTRFAAEIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDEMVCSQENGTVQIKGETVYKVTDVIDVTIAEVRMETRSIIA
RPVA

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3' to 5' direction [H]

COG id: COG4776

COG function: function code K; Exoribonuclease II

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI190014623, Length=387, Percent_Identity=25.8397932816537, Blast_Score=116, Evalue=8e-26,
Organism=Homo sapiens, GI190014625, Length=387, Percent_Identity=25.8397932816537, Blast_Score=115, Evalue=9e-26,
Organism=Homo sapiens, GI134288890, Length=365, Percent_Identity=29.041095890411, Blast_Score=103, Evalue=6e-22,
Organism=Homo sapiens, GI219521928, Length=456, Percent_Identity=24.780701754386, Blast_Score=94, Evalue=5e-19,
Organism=Homo sapiens, GI19115966, Length=456, Percent_Identity=24.780701754386, Blast_Score=93, Evalue=7e-19,
Organism=Escherichia coli, GI1787542, Length=644, Percent_Identity=99.6894409937888, Blast_Score=1320, Evalue=0.0,
Organism=Escherichia coli, GI87082383, Length=651, Percent_Identity=26.1136712749616, Blast_Score=174, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI212645896, Length=467, Percent_Identity=26.1241970021413, Blast_Score=118, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI17553506, Length=409, Percent_Identity=27.1393643031785, Blast_Score=115, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6324552, Length=420, Percent_Identity=24.047619047619, Blast_Score=101, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24649634, Length=370, Percent_Identity=27.8378378378378, Blast_Score=122, Evalue=6e-28,
Organism=Drosophila melanogaster, GI24654592, Length=451, Percent_Identity=26.6075388026608, Blast_Score=117, Evalue=3e-26,
Organism=Drosophila melanogaster, GI19922976, Length=451, Percent_Identity=26.6075388026608, Blast_Score=116, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24654597, Length=451, Percent_Identity=26.6075388026608, Blast_Score=116, Evalue=4e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011129
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013223
- InterPro:   IPR001900
- InterPro:   IPR022966
- InterPro:   IPR004476
- InterPro:   IPR011804 [H]

Pfam domain/function: PF08206 OB_RNB; PF00773 RNB; PF00575 S1 [H]

EC number: =3.1.13.1 [H]

Molecular weight: Translated: 72510; Mature: 72510

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS01175 RIBONUCLEASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSYFIPPPQMKKVMHGDRI
CCCCCHHHHHHHHHHHHCCCCCCCCCEECCCCCEEEEECCCCCCCCCCHHHHHHHCCCEE
IAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPDHPLLKDAIPCRAARGLNH
EEEEECCHHHCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHCCCHHHHCCCCC
EFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVPWWVTLARHNLEKEAPDGVAT
CCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCHHH
EMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALPDDKLQLIVAIADPTAWIAEGSK
HHHHCCHHHHCCCHHEEEEECCCCCCCHHHHHHHHCCCCCCEEEEEEEECCCHHHHCCCC
LDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRANEVRPVLACRMTLSADGTIEDNIEF
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCEEHHEEEEECCCCCCCCCHHH
FAATIESKAKLVYDQVSDWLENTGDWKPESEAIAEQVRLLAQICQRRGEWRHNHALVFKD
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEC
RPDYRFILGEKGEVLDIVAEPRRIANRIVEEAMIAANICAARVLRDKLGFGIYNVHMGFD
CCCEEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCC
PANADALAALLKTHGLHVDAEEVLTLDGFCKLRRELDAQPTGFLDSRIRRFQSFAEISTE
CCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCC
PGPHFGLGLEAYATWTSPIRKYGDMINHRLLKAVIKGETATRPQDEITVQMAERRRLNRM
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
AERDVGDWLYARFLKDKAGTDTRFAAEIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDE
HHHCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEEEECCCEEEECHHHHHHHHHH
MVCSQENGTVQIKGETVYKVTDVIDVTIAEVRMETRSIIARPVA
HHCCCCCCEEEEECCEEEEEHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSYFIPPPQMKKVMHGDRI
CCCCCHHHHHHHHHHHHCCCCCCCCCEECCCCCEEEEECCCCCCCCCCHHHHHHHCCCEE
IAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPDHPLLKDAIPCRAARGLNH
EEEEECCHHHCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHCCCHHHHCCCCC
EFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVPWWVTLARHNLEKEAPDGVAT
CCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCHHH
EMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALPDDKLQLIVAIADPTAWIAEGSK
HHHHCCHHHHCCCHHEEEEECCCCCCCHHHHHHHHCCCCCCEEEEEEEECCCHHHHCCCC
LDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRANEVRPVLACRMTLSADGTIEDNIEF
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCEEHHEEEEECCCCCCCCCHHH
FAATIESKAKLVYDQVSDWLENTGDWKPESEAIAEQVRLLAQICQRRGEWRHNHALVFKD
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEC
RPDYRFILGEKGEVLDIVAEPRRIANRIVEEAMIAANICAARVLRDKLGFGIYNVHMGFD
CCCEEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCC
PANADALAALLKTHGLHVDAEEVLTLDGFCKLRRELDAQPTGFLDSRIRRFQSFAEISTE
CCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCC
PGPHFGLGLEAYATWTSPIRKYGDMINHRLLKAVIKGETATRPQDEITVQMAERRRLNRM
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
AERDVGDWLYARFLKDKAGTDTRFAAEIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDE
HHHCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEEEECCCEEEECHHHHHHHHHH
MVCSQENGTVQIKGETVYKVTDVIDVTIAEVRMETRSIIARPVA
HHCCCCCCEEEEECCEEEEEHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA