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The map label for this gene is purU

Identifier: 157160738

GI number: 157160738

Start: 1336040

End: 1336882

Strand: Reverse

Name: purU

Synonym: EcHS_A1340

Alternate gene names: 157160738

Gene position: 1336882-1336040 (Counterclockwise)

Preceding gene: 157160739

Following gene: 157160731

Centisome position: 28.79

GC content: 47.45

Gene sequence:

>843_bases
ATGCATTCACTCCAACGTAAAGTTCTGCGTACTATTTGTCCGGACCAAAAAGGTCTGATCGCACGTATTACCAATATTTG
CTACAAGCACGAGTTAAATATCGTACAGAACAATGAATTTGTTGATCACCGTACCGGGCGCTTTTTTATGCGCACGGAAC
TGGAAGGGATTTTTAATGATTCCACCCTGCTGGCGGATCTCGATAGCGCATTGCCAGAAGGCTCCGTGCGTGAGCTGAAT
CCTGCCGGTCGTCGCCGGATAGTGATTCTGGTCACTAAAGAAGCGCATTGCCTTGGCGATTTGTTGATGAAAGCCAATTA
CGGCGGCCTGGATGTCGAAATCGCGGCAGTTATTGGTAACCACGATACTCTACGTTCTCTGGTTGAGCGTTTTGATATTC
CGTTTGAGCTGGTAAGCCATGAAGGGTTAAGCCGCAACGAGCACGATCAAAAGATGGCGGATGCCATTGATGCTTATCAA
CCTGACTACGTGGTGCTGGCGAAGTATATGCGGGTATTAACGCCGGAATTTGTGGCACGCTTCCCGAATAAGATCATCAA
TATTCACCATTCATTCCTGCCAGCGTTTATTGGCGCACGTCCTTATCACCAGGCCTATGAACGTGGTGTGAAGATTATTG
GCGCAACCGCTCACTATGTGAATGACAATCTGGACGAAGGCCCAATCATCATGCAGGACGTTATTCATGTCGATCATACC
TACACAGCTGAAGATATGATGCGCGCAGGTCGTGACGTCGAGAAAAACGTCTTAAGTCGTGCACTATACAAAGTACTGGC
ACAGCGCGTCTTTGTTTACGGTAATCGGACGATTATTCTTTAA

Upstream 100 bases:

>100_bases
CCCTGCCCTTGTGGTTCAGGTAAAAAATTTAAAAAGTGCTGCGGCCAATAATGGTTGACGGTACGGTTTAGCAAACACTC
TCAACAAGGTTTTTCCAGCA

Downstream 100 bases:

>100_bases
TCGCCAGCAAAAATAACTGGTTACCTTTAATCCGTTACGGATGAAAATTACGCAACCAGTTCATTTTTCTCAACGTAACA
CTTTACAGCGGCGCGTCATT

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHRTGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELN
PAGRRRIVILVTKEAHCLGDLLMKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAHYVNDNLDEGPIIMQDVIHVDHT
YTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNRTIIL

Sequences:

>Translated_280_residues
MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHRTGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELN
PAGRRRIVILVTKEAHCLGDLLMKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAHYVNDNLDEGPIIMQDVIHVDHT
YTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNRTIIL
>Mature_280_residues
MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHRTGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELN
PAGRRRIVILVTKEAHCLGDLLMKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAHYVNDNLDEGPIIMQDVIHVDHT
YTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNRTIIL

Specific function: Produces formate from formyl-tetrahydrofolate. Provides the major source of formate for the purT-dependent synthesis of 5'-phosphoribosyl-N-formylglycinamide (FGAR) during aerobic growth. Has a role in regulating the one-carbon pool

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain

Homologues:

Organism=Homo sapiens, GI4503915, Length=190, Percent_Identity=29.4736842105263, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI209869995, Length=190, Percent_Identity=29.4736842105263, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI209869993, Length=190, Percent_Identity=29.4736842105263, Blast_Score=92, Evalue=7e-19,
Organism=Escherichia coli, GI1787483, Length=280, Percent_Identity=99.6428571428571, Blast_Score=576, Evalue=1e-166,
Organism=Escherichia coli, GI1788846, Length=94, Percent_Identity=42.5531914893617, Blast_Score=87, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17567511, Length=187, Percent_Identity=31.5508021390374, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24582400, Length=159, Percent_Identity=30.188679245283, Blast_Score=80, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PURU_ECOL6 (P0A440)

Other databases:

- EMBL:   AE014075
- RefSeq:   NP_753601.1
- ProteinModelPortal:   P0A440
- SMR:   P0A440
- EnsemblBacteria:   EBESCT00000040485
- GeneID:   1035478
- GenomeReviews:   AE014075_GR
- KEGG:   ecc:c1696
- GeneTree:   EBGT00050000009381
- HOGENOM:   HBG286016
- OMA:   WIVEAAY
- ProtClustDB:   PRK06027
- BRENDA:   3.5.1.10
- InterPro:   IPR002912
- InterPro:   IPR002376
- InterPro:   IPR004810
- Gene3D:   G3DSA:3.40.50.170
- PIRSF:   PIRSF036480
- PRINTS:   PR01575
- TIGRFAMs:   TIGR00655

Pfam domain/function: PF01842 ACT; PF00551 Formyl_trans_N; SSF53328 formyl_transf

EC number: =3.5.1.10

Molecular weight: Translated: 31921; Mature: 31921

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: NA

Important sites: ACT_SITE 225-225

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHRTGRFFMRTELEGIFND
CCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCCEEEEEHHHHHCCC
STLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLLMKANYGGLDVEIAAVIGN
CHHHHHHHHHCCCCCCHHCCCCCCEEEEEEEECCHHHHHHHHHHCCCCCCCEEEEEEECC
HDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQPDYVVLAKYMRVLTPEFVAR
HHHHHHHHHHHCCCHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHH
FPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAHYVNDNLDEGPIIMQDVIHVDHT
CCHHHHHHHHHHHHHHHCCCCHHHHHHCCCEEEEEEHHHHCCCCCCCCHHHHHHHHHCCC
YTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNRTIIL
CCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEC
>Mature Secondary Structure
MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHRTGRFFMRTELEGIFND
CCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCCEEEEEHHHHHCCC
STLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLLMKANYGGLDVEIAAVIGN
CHHHHHHHHHCCCCCCHHCCCCCCEEEEEEEECCHHHHHHHHHHCCCCCCCEEEEEEECC
HDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQPDYVVLAKYMRVLTPEFVAR
HHHHHHHHHHHCCCHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHH
FPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAHYVNDNLDEGPIIMQDVIHVDHT
CCHHHHHHHHHHHHHHHCCCCHHHHHHCCCEEEEEEHHHHCCCCCCCCHHHHHHHHHCCC
YTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNRTIIL
CCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157