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The map label for this gene is prfA [H]

Identifier: 157160716

GI number: 157160716

Start: 1313451

End: 1314533

Strand: Direct

Name: prfA [H]

Synonym: EcHS_A1316

Alternate gene names: 157160716

Gene position: 1313451-1314533 (Clockwise)

Preceding gene: 157160715

Following gene: 157160717

Centisome position: 28.29

GC content: 53.0

Gene sequence:

>1083_bases
ATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCA
AACTATCGCCGACCAGGAACGTTTTCGCGCATTATCACGCGAATATGCGCAGTTAAGTGATGTTTCGCGCTGTTTTACCG
ACTGGCAACAGGTTCAGGAAGATATCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGAT
GAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGA
CGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTA
TGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAA
GAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCGGTCATCGCGTGCAACGTGT
TCCTGCTACGGAATCGCAGGGTCGTATTCATACTTCTGCTTGTACCGTTGCGGTAATGCCAGAACTGCCTGACGCAGAAC
TGCCGGACATCAACCCAGCAGATTTACGCATTGATACTTTCCGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACACCACC
GATTCGGCAATTCGTATTACTCACTTGCCGACCGGGATTGTTGTTGAATGTCAGGACGAACGTTCACAACATAAAAACAA
AGCTAAAGCACTTTCTGTTCTCGGTGCTCGCATCCACGCTGCTGAAATGGCAAAACGCCAACAGGCCGAAGCGTCTACCC
GTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTAACCGTACTTACAACTTCCCGCAGGGGCGCGTTACCGATCAC
CGCATCAACCTGACGCTCTACCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATGCTGATTGAACCGATTATCCAGGA
ACATCAGGCCGACCAACTGGCGGCGTTGTCCGAGCAGGAATAA

Upstream 100 bases:

>100_bases
CCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTT
TACAGGGTGCATTTACGCCT

Downstream 100 bases:

>100_bases
TGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTG
CTGGAGCATGTTACCGGCAG

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQLSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQD
ELREAKEKSEQLEQQLQVLLLPKDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDINPADLRIDTFRSSGAGGQHVNTT
DSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGARIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDH
RINLTLYRLDEVMEGKLDMLIEPIIQEHQADQLAALSEQE

Sequences:

>Translated_360_residues
MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQLSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQD
ELREAKEKSEQLEQQLQVLLLPKDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDINPADLRIDTFRSSGAGGQHVNTT
DSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGARIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDH
RINLTLYRLDEVMEGKLDMLIEPIIQEHQADQLAALSEQE
>Mature_360_residues
MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQLSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQD
ELREAKEKSEQLEQQLQVLLLPKDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDINPADLRIDTFRSSGAGGQHVNTT
DSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGARIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDH
RINLTLYRLDEVMEGKLDMLIEPIIQEHQADQLAALSEQE

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=285, Percent_Identity=48.4210526315789, Blast_Score=273, Evalue=1e-73,
Organism=Homo sapiens, GI34577120, Length=354, Percent_Identity=39.8305084745763, Blast_Score=245, Evalue=4e-65,
Organism=Homo sapiens, GI166795305, Length=190, Percent_Identity=50, Blast_Score=189, Evalue=3e-48,
Organism=Escherichia coli, GI1787462, Length=360, Percent_Identity=100, Blast_Score=741, Evalue=0.0,
Organism=Escherichia coli, GI2367172, Length=342, Percent_Identity=39.766081871345, Blast_Score=216, Evalue=3e-57,
Organism=Caenorhabditis elegans, GI17542784, Length=342, Percent_Identity=32.4561403508772, Blast_Score=182, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6321295, Length=313, Percent_Identity=43.7699680511182, Blast_Score=260, Evalue=2e-70,
Organism=Drosophila melanogaster, GI19921226, Length=327, Percent_Identity=39.1437308868502, Blast_Score=207, Evalue=1e-53,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 40518; Mature: 40518

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQLSDVSRCFTDWQQVQE
CCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLPKDPDDERNAFLEVRAGTG
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCEEEEEECCCC
GDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYKEIIAKISGDGVYGRLKFESG
CCCHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCHHHHHHHHCCCCEEEEEEECCC
GHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDINPADLRIDTFRSSGAGGQHVNTT
CCEEEECCCCCCCCEEEECEEEEEECCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCC
DSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGARIHAAEMAKRQQAEASTRRNLLGS
CCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVMEGKLDMLIEPIIQEHQADQLAALSEQE
CCCCCCCCCCCCCCCCCCCEEEEEEEEEHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCC
>Mature Secondary Structure
MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQLSDVSRCFTDWQQVQE
CCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLPKDPDDERNAFLEVRAGTG
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCEEEEEECCCC
GDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYKEIIAKISGDGVYGRLKFESG
CCCHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCHHHHHHHHCCCCEEEEEEECCC
GHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDINPADLRIDTFRSSGAGGQHVNTT
CCEEEECCCCCCCCEEEECEEEEEECCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCC
DSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGARIHAAEMAKRQQAEASTRRNLLGS
CCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVMEGKLDMLIEPIIQEHQADQLAALSEQE
CCCCCCCCCCCCCCCCCCCEEEEEEEEEHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA