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The map label for this gene is prs

Identifier: 157160712

GI number: 157160712

Start: 1309367

End: 1310314

Strand: Reverse

Name: prs

Synonym: EcHS_A1312

Alternate gene names: 157160712

Gene position: 1310314-1309367 (Counterclockwise)

Preceding gene: 157160713

Following gene: 157160711

Centisome position: 28.22

GC content: 52.53

Gene sequence:

>948_bases
GTGCCTGATATGAAGCTTTTTGCTGGTAACGCCACCCCGGAACTAGCACAACGTATTGCCAACCGCCTGTACACTTCACT
CGGCGACGCCGCTGTAGGTCGCTTTAGCGATGGCGAAGTCAGCGTACAAATTAATGAAAATGTACGCGGTGGTGATATTT
TCATCATCCAGTCCACTTGTGCCCCTACTAACGACAACCTGATGGAATTAGTCGTTATGGTTGATGCCCTGCGTCGTGCT
TCCGCAGGTCGTATCACCGCTGTTATCCCCTACTTTGGCTATGCGCGCCAGGACCGTCGCGTCCGTTCCGCTCGTGTACC
AATCACTGCGAAAGTGGTTGCAGACTTCCTCTCCAGCGTCGGTGTTGACCGTGTGCTGACAGTGGATCTGCACGCTGAAC
AGATTCAGGGTTTCTTCGACGTTCCGGTTGATAACGTATTTGGTAGCCCGATCCTGCTGGAAGACATGCTGCAGCTGAAT
CTGGATAACCCAATTGTGGTTTCTCCGGACATCGGCGGCGTTGTGCGTGCCCGCGCTATCGCTAAGCTGCTGAACGATAC
CGATATGGCAATCATCGACAAACGTCGTCCGCGTGCGAACGTTTCACAGGTGATGCATATCATCGGTGACGTTGCAGGTC
GTGACTGCGTACTGGTCGATGATATGATCGACACTGGCGGTACGCTGTGTAAAGCTGCTGAAGCTCTGAAAGAACGTGGT
GCTAAACGTGTATTTGCGTACGCGACTCACCCGATCTTCTCTGGCAACGCGGCGAACAACCTGCGTAACTCTGTAATTGA
TGAAGTCGTTGTCTGCGATACCATTCCGCTGAGCGATGAAATCAAATCACTGCCGAACGTGCGTACTCTGACCCTGTCAG
GTATGCTGGCCGAAGCGATTCGTCGTATCAGCAACGAAGAATCGATCTCTGCCATGTTCGAACACTAA

Upstream 100 bases:

>100_bases
ATCGCGCTCTTTAATACACCGCCTGGAAAGGATCATGCCTGGCCCGCACAGTTTTCGGCAGATTCTTTCCACCAATGGAC
GCATGCCTGAGGTTCTTCTC

Downstream 100 bases:

>100_bases
TCGAACCCGGCTCAAAGACCCGCTGCGGCGGGTTTTTTTGTCTGTAATATCCATTTGTATGACCTATGCCTCCTTCACCT
GCCATTTAGTTGACAGATGA

Product: ribose-phosphate pyrophosphokinase

Products: NA

Alternate protein names: RPPK; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase

Number of amino acids: Translated: 315; Mature: 314

Protein sequence:

>315_residues
MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQINENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRA
SAGRITAVIPYFGYARQDRRVRSARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVLVDDMIDTGGTLCKAAEALKERG
AKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIPLSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH

Sequences:

>Translated_315_residues
MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQINENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRA
SAGRITAVIPYFGYARQDRRVRSARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVLVDDMIDTGGTLCKAAEALKERG
AKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIPLSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH
>Mature_314_residues
PDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQINENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRAS
AGRITAVIPYFGYARQDRRVRSARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLNL
DNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVLVDDMIDTGGTLCKAAEALKERGA
KRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIPLSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH

Specific function: Utilized by both the de novo and the salvage pathways by which endogenously formed or exogenously added pyrimidine, purine, or pyridine bases are converted to the corresponding ribonucleoside monophosphates. [C]

COG id: COG0462

COG function: function code FE; Phosphoribosylpyrophosphate synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribose-phosphate pyrophosphokinase family

Homologues:

Organism=Homo sapiens, GI4506129, Length=317, Percent_Identity=48.2649842271293, Blast_Score=303, Evalue=2e-82,
Organism=Homo sapiens, GI4506127, Length=318, Percent_Identity=48.4276729559748, Blast_Score=303, Evalue=2e-82,
Organism=Homo sapiens, GI84875539, Length=319, Percent_Identity=47.9623824451411, Blast_Score=302, Evalue=2e-82,
Organism=Homo sapiens, GI28557709, Length=317, Percent_Identity=47.3186119873817, Blast_Score=295, Evalue=4e-80,
Organism=Homo sapiens, GI4506133, Length=343, Percent_Identity=36.1516034985423, Blast_Score=196, Evalue=3e-50,
Organism=Homo sapiens, GI194018537, Length=343, Percent_Identity=36.734693877551, Blast_Score=194, Evalue=1e-49,
Organism=Homo sapiens, GI310128524, Length=153, Percent_Identity=32.6797385620915, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI310115209, Length=153, Percent_Identity=32.6797385620915, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI310118259, Length=153, Percent_Identity=32.6797385620915, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI310119946, Length=153, Percent_Identity=32.6797385620915, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI1787458, Length=315, Percent_Identity=100, Blast_Score=635, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25149168, Length=317, Percent_Identity=46.6876971608833, Blast_Score=296, Evalue=7e-81,
Organism=Caenorhabditis elegans, GI17554702, Length=317, Percent_Identity=46.6876971608833, Blast_Score=296, Evalue=9e-81,
Organism=Caenorhabditis elegans, GI71989924, Length=317, Percent_Identity=46.6876971608833, Blast_Score=295, Evalue=2e-80,
Organism=Caenorhabditis elegans, GI17554704, Length=312, Percent_Identity=46.474358974359, Blast_Score=288, Evalue=2e-78,
Organism=Caenorhabditis elegans, GI17570245, Length=339, Percent_Identity=34.2182890855457, Blast_Score=194, Evalue=5e-50,
Organism=Saccharomyces cerevisiae, GI6319403, Length=316, Percent_Identity=46.5189873417722, Blast_Score=271, Evalue=9e-74,
Organism=Saccharomyces cerevisiae, GI6321776, Length=319, Percent_Identity=47.3354231974922, Blast_Score=270, Evalue=2e-73,
Organism=Saccharomyces cerevisiae, GI6320946, Length=315, Percent_Identity=46.3492063492063, Blast_Score=268, Evalue=8e-73,
Organism=Saccharomyces cerevisiae, GI6322667, Length=206, Percent_Identity=40.2912621359223, Blast_Score=148, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6324511, Length=115, Percent_Identity=40, Blast_Score=96, Evalue=6e-21,
Organism=Drosophila melanogaster, GI21355239, Length=317, Percent_Identity=47.0031545741325, Blast_Score=291, Evalue=5e-79,
Organism=Drosophila melanogaster, GI45551540, Length=339, Percent_Identity=43.952802359882, Blast_Score=282, Evalue=2e-76,
Organism=Drosophila melanogaster, GI24651458, Length=355, Percent_Identity=34.6478873239437, Blast_Score=197, Evalue=8e-51,
Organism=Drosophila melanogaster, GI24651456, Length=355, Percent_Identity=34.6478873239437, Blast_Score=197, Evalue=8e-51,
Organism=Drosophila melanogaster, GI281362873, Length=355, Percent_Identity=34.6478873239437, Blast_Score=197, Evalue=1e-50,
Organism=Drosophila melanogaster, GI24651454, Length=355, Percent_Identity=34.6478873239437, Blast_Score=197, Evalue=1e-50,
Organism=Drosophila melanogaster, GI45552010, Length=374, Percent_Identity=32.8877005347594, Blast_Score=191, Evalue=5e-49,
Organism=Drosophila melanogaster, GI24651462, Length=374, Percent_Identity=32.8877005347594, Blast_Score=191, Evalue=5e-49,
Organism=Drosophila melanogaster, GI24651464, Length=374, Percent_Identity=32.8877005347594, Blast_Score=191, Evalue=5e-49,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): KPRS_ECO57 (P0A719)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   H90842
- RefSeq:   NP_287453.1
- RefSeq:   NP_309739.1
- ProteinModelPortal:   P0A719
- SMR:   P0A719
- MINT:   MINT-1221661
- EnsemblBacteria:   EBESCT00000025656
- EnsemblBacteria:   EBESCT00000057962
- GeneID:   913151
- GeneID:   960457
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z1978
- KEGG:   ecs:ECs1712
- GeneTree:   EBGT00050000011468
- HOGENOM:   HBG519284
- OMA:   NTIPQDG
- ProtClustDB:   PRK01259
- BioCyc:   ECOL83334:ECS1712-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00583_B
- InterPro:   IPR000842
- InterPro:   IPR005946
- InterPro:   IPR000836
- TIGRFAMs:   TIGR01251

Pfam domain/function: PF00156 Pribosyltran

EC number: =2.7.6.1

Molecular weight: Translated: 34219; Mature: 34088

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00114 PRPP_SYNTHASE; PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQINENVRGGDIFIIQSTC
CCCCEEEECCCCHHHHHHHHHHHHHHHCCHHHCCCCCCEEEEEECCCCCCCEEEEEECCC
APTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVRSARVPITAKVVADFLSSV
CCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHC
GVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLNLDNPIVVSPDIGGVVRARAI
CCCEEEEEEEEHHHHCCEECCCHHHHCCCHHHHHHHHHHCCCCCEEECCCCCHHHHHHHH
AKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVLVDDMIDTGGTLCKAAEALKERG
HHHHCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEHHHHHCCCHHHHHHHHHHHCC
AKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIPLSDEIKSLPNVRTLTLSGMLAEAI
CCEEEEEECCCEECCCCHHHHHHHHHHHHHHCCCCCCCHHHHHCCCEEEEEHHHHHHHHH
RRISNEESISAMFEH
HHHCCHHHHHHHHCC
>Mature Secondary Structure 
PDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQINENVRGGDIFIIQSTC
CCCEEEECCCCHHHHHHHHHHHHHHHCCHHHCCCCCCEEEEEECCCCCCCEEEEEECCC
APTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVRSARVPITAKVVADFLSSV
CCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHC
GVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLNLDNPIVVSPDIGGVVRARAI
CCCEEEEEEEEHHHHCCEECCCHHHHCCCHHHHHHHHHHCCCCCEEECCCCCHHHHHHHH
AKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVLVDDMIDTGGTLCKAAEALKERG
HHHHCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEHHHHHCCCHHHHHHHHHHHCC
AKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIPLSDEIKSLPNVRTLTLSGMLAEAI
CCEEEEEECCCEECCCCHHHHHHHHHHHHHHCCCCCCCHHHHHCCCEEEEEHHHHHHHHH
RRISNEESISAMFEH
HHHCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796