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The map label for this gene is ychM

Identifier: 157160711

GI number: 157160711

Start: 1307563

End: 1309215

Strand: Reverse

Name: ychM

Synonym: EcHS_A1311

Alternate gene names: 157160711

Gene position: 1309215-1307563 (Counterclockwise)

Preceding gene: 157160712

Following gene: 157160709

Centisome position: 28.19

GC content: 54.63

Gene sequence:

>1653_bases
ATGCCTTTCCGCGCTCTGATCGACGCTTGCTGGAAAGAAAAATATACTGCCGCACGGTTTACCCGTGACCTGATTGCCGG
GATAACCGTCGGGATTATTGCTATCCCGCTGGCGATGGCGTTGGCTATTGGTAGTGGTGTGGCACCCCAGTACGGTTTAT
ATACCGCAGCTGTTGCGGGGATTGTCATTGCTCTGACGGGTGGGTCACGCTTTAGCGTTTCCGGTCCGACTGCGGCATTT
GTGGTAATTCTCTATCCCGTGTCGCAACAGTTTGGACTGGCAGGACTGCTGGTTGCGACCTTGCTGTCGGGGATCTTTTT
GATTCTGATGGGTCTGGCACGCTTTGGTCGCCTGATTGAGTATATTCCGGTTTCCGTCACCTTAGGTTTCACCTCGGGTA
TCGGGATCACCATCGGTACCATGCAGATTAAAGATTTTCTCGGTCTGCAAATGGCCCATGTCCCGGAACATTATCTACAA
AAAGTCGGCGCATTATTTATGGCGCTGCCGACCATTAATGTGGGTGATGCTGCCATTGGCATTGTGACGCTAGGTATTCT
TGTTTTTTGGCCGCGTCTGGGCATTCGTTTACCCGGTCACCTTCCGGCCTTGCTGGCTGGTTGCGCGGTGATGGGGATTG
TTAACCTGCTCGGCGGACATGTTGCTACCATCGGTTCGCAATTCCACTACGTCCTGGCCGATGGTTCTCAGGGTAACGGT
ATTCCGCAACTGCTGCCGCAACTGGTGCTGCCGTGGGATCTGCCTAATTCAGAATTCACGCTAACCTGGGATTCTATTCG
CACACTGCTGCCTGCGGCATTCTCAATGGCAATGCTCGGCGCAATCGAATCTCTGCTCTGCGCCGTGGTACTGGATGGTA
TGACCGGGACGAAACACAAGGCGAACAGCGAACTGGTTGGACAGGGACTGGGGAATATTATCGCTCCGTTCTTTGGTGGT
ATTACCGCTACAGCTGCCATCGCGCGTTCTGCCGCTAACGTCCGTGCCGGGGCAACTTCCCCTATCTCGGCGGTGATCCA
CTCTATTCTGGTTATTCTTGCCCTGCTGGTACTGGCACCGCTGCTCTCCTGGCTGCCGCTTTCCGCTATGGCAGCCCTGC
TGTTGATGGTGGCGTGGAACATGAGTGAAGCGCATAAAGTGGTCGACTTGCTGCGTCATGCACCGAAAGATGACATCATT
GTCATGCTGCTGTGCATGTCGCTGACCGTGCTGTTTGATATGGTTATTGCCATCAGCGTGGGGATCGTGCTGGCATCGCT
GCTGTTTATGCGTCGTATCGCACGTATGACTCGCCTGGCACCGGTAGTCGTAGATGTTCCAGACGATGTTCTGGTACTGC
GCGTTATTGGCCCGCTGTTTTTTGCTGCTGCTGAAGGCTTGTTCACGGACCTGGAGTCACGTCTTGAAGGCAAACGGATT
GTGATTCTGAAGTGGGATGCCGTTCCGGTACTTGATGCTGGTGGTCTTGATGCGTTCCAGCGTTTTGTGAAGCGTCTGCC
CGAAGGATGTGAACTGCGCGTGTGCAACGTGGAATTCCAGCCACTGCGCACTATGGCTCGCGCAGGCATTCAACCGATCC
CGGGACGCCTCGCGTTCTTCCCGAATCGTCGCGCGGCGATGGCGGATTTATAA

Upstream 100 bases:

>100_bases
CATTTGTATGACCTATGCCTCCTTCACCTGCCATTTAGTTGACAGATGATGCGCTCACGGATGAAACATTATTGTGAACA
AAATATTTTCCTCACATGTG

Downstream 100 bases:

>100_bases
GTACGAGATTGACCAGTCAGCGCAAACTGACTGGTCAGCAAACTGCATTATTTGTTAGCTATGACGGCGATTATCGCTAC
GGCGGCAACGTTTGTCATAG

Product: putative sulfate transporter YchM

Products: Proton [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 550; Mature: 549

Protein sequence:

>550_residues
MPFRALIDACWKEKYTAARFTRDLIAGITVGIIAIPLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAF
VVILYPVSQQFGLAGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQMAHVPEHYLQ
KVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCAVMGIVNLLGGHVATIGSQFHYVLADGSQGNG
IPQLLPQLVLPWDLPNSEFTLTWDSIRTLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGG
ITATAAIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSEAHKVVDLLRHAPKDDII
VMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAPVVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRI
VILKWDAVPVLDAGGLDAFQRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL

Sequences:

>Translated_550_residues
MPFRALIDACWKEKYTAARFTRDLIAGITVGIIAIPLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAF
VVILYPVSQQFGLAGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQMAHVPEHYLQ
KVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCAVMGIVNLLGGHVATIGSQFHYVLADGSQGNG
IPQLLPQLVLPWDLPNSEFTLTWDSIRTLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGG
ITATAAIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSEAHKVVDLLRHAPKDDII
VMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAPVVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRI
VILKWDAVPVLDAGGLDAFQRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL
>Mature_549_residues
PFRALIDACWKEKYTAARFTRDLIAGITVGIIAIPLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFV
VILYPVSQQFGLAGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQMAHVPEHYLQK
VGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCAVMGIVNLLGGHVATIGSQFHYVLADGSQGNGI
PQLLPQLVLPWDLPNSEFTLTWDSIRTLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGI
TATAAIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSEAHKVVDLLRHAPKDDIIV
MLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAPVVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIV
ILKWDAVPVLDAGGLDAFQRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL

Specific function: Possible sulfate transporter

COG id: COG0659

COG function: function code P; Sulfate permease and related transporters (MFS superfamily)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 STAS domain

Homologues:

Organism=Homo sapiens, GI262206105, Length=503, Percent_Identity=27.037773359841, Blast_Score=141, Evalue=2e-33,
Organism=Homo sapiens, GI262206075, Length=503, Percent_Identity=27.037773359841, Blast_Score=141, Evalue=2e-33,
Organism=Homo sapiens, GI262206069, Length=503, Percent_Identity=27.037773359841, Blast_Score=141, Evalue=2e-33,
Organism=Homo sapiens, GI262206063, Length=503, Percent_Identity=27.037773359841, Blast_Score=141, Evalue=2e-33,
Organism=Homo sapiens, GI4557535, Length=489, Percent_Identity=23.1083844580777, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI94721257, Length=482, Percent_Identity=23.2365145228216, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI94721259, Length=482, Percent_Identity=23.2365145228216, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI94721253, Length=482, Percent_Identity=23.2365145228216, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI94721255, Length=482, Percent_Identity=23.2365145228216, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI39752683, Length=389, Percent_Identity=22.879177377892, Blast_Score=80, Evalue=6e-15,
Organism=Homo sapiens, GI45827800, Length=389, Percent_Identity=22.879177377892, Blast_Score=79, Evalue=8e-15,
Organism=Homo sapiens, GI4505697, Length=433, Percent_Identity=23.094688221709, Blast_Score=79, Evalue=9e-15,
Organism=Homo sapiens, GI45827802, Length=402, Percent_Identity=21.8905472636816, Blast_Score=79, Evalue=9e-15,
Organism=Homo sapiens, GI269784651, Length=379, Percent_Identity=23.2189973614776, Blast_Score=77, Evalue=6e-14,
Organism=Homo sapiens, GI20336282, Length=510, Percent_Identity=21.5686274509804, Blast_Score=76, Evalue=8e-14,
Organism=Homo sapiens, GI16306483, Length=510, Percent_Identity=21.5686274509804, Blast_Score=76, Evalue=8e-14,
Organism=Homo sapiens, GI47131207, Length=526, Percent_Identity=23.7642585551331, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI20336272, Length=526, Percent_Identity=23.7642585551331, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI100913030, Length=478, Percent_Identity=23.0125523012552, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI87081859, Length=550, Percent_Identity=100, Blast_Score=1085, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17566848, Length=569, Percent_Identity=24.780316344464, Blast_Score=102, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI86564196, Length=445, Percent_Identity=24.2696629213483, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI193203292, Length=384, Percent_Identity=23.1770833333333, Blast_Score=69, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6323121, Length=456, Percent_Identity=23.9035087719298, Blast_Score=91, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6325260, Length=551, Percent_Identity=22.3230490018149, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6319771, Length=193, Percent_Identity=30.0518134715026, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI85815873, Length=479, Percent_Identity=25.8872651356994, Blast_Score=118, Evalue=1e-26,
Organism=Drosophila melanogaster, GI19922482, Length=423, Percent_Identity=25.531914893617, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24663084, Length=443, Percent_Identity=27.765237020316, Blast_Score=116, Evalue=4e-26,
Organism=Drosophila melanogaster, GI21357695, Length=443, Percent_Identity=27.765237020316, Blast_Score=116, Evalue=4e-26,
Organism=Drosophila melanogaster, GI21358229, Length=463, Percent_Identity=26.5658747300216, Blast_Score=112, Evalue=7e-25,
Organism=Drosophila melanogaster, GI24649801, Length=453, Percent_Identity=26.4900662251656, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24651449, Length=448, Percent_Identity=24.1071428571429, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI21358633, Length=434, Percent_Identity=25.8064516129032, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24647160, Length=178, Percent_Identity=30.3370786516854, Blast_Score=79, Evalue=9e-15,
Organism=Drosophila melanogaster, GI21355087, Length=178, Percent_Identity=30.3370786516854, Blast_Score=79, Evalue=9e-15,
Organism=Drosophila melanogaster, GI24666186, Length=442, Percent_Identity=25.3393665158371, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YCHM_ECO57 (P0AFR3)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   D85700
- PIR:   G90842
- RefSeq:   NP_287452.1
- RefSeq:   NP_309738.1
- ProteinModelPortal:   P0AFR3
- SMR:   P0AFR3
- EnsemblBacteria:   EBESCT00000025139
- EnsemblBacteria:   EBESCT00000055341
- GeneID:   913152
- GeneID:   960456
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z1977
- KEGG:   ecs:ECs1711
- GeneTree:   EBGT00050000010289
- HOGENOM:   HBG564176
- OMA:   VFLTCFS
- ProtClustDB:   PRK11660
- BioCyc:   ECOL83334:ECS1711-MONOMER
- InterPro:   IPR018045
- InterPro:   IPR002645
- InterPro:   IPR001902
- InterPro:   IPR011547
- Gene3D:   G3DSA:3.30.750.24
- TIGRFAMs:   TIGR00815

Pfam domain/function: PF01740 STAS; PF00916 Sulfate_transp; SSF52091 STAS

EC number: NA

Molecular weight: Translated: 58386; Mature: 58255

Theoretical pI: Translated: 8.80; Mature: 8.80

Prosite motif: PS01130 SLC26A; PS50801 STAS

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x14045438)-; HASH(0x14568928)-; HASH(0x12a3d9ac)-; HASH(0x14553078)-; HASH(0x1456ee1c)-; HASH(0x145be9e8)-; HASH(0x11952d44)-; HASH(0x13e07208)-; HASH(0x14568c04)-; HASH(0x14553698)-;

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFRALIDACWKEKYTAARFTRDLIAGITVGIIAIPLAMALAIGSGVAPQYGLYTAAVAG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
IVIALTGGSRFSVSGPTAAFVVILYPVSQQFGLAGLLVATLLSGIFLILMGLARFGRLIE
HEEEEECCCEEECCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YIPVSVTLGFTSGIGITIGTMQIKDFLGLQMAHVPEHYLQKVGALFMALPTINVGDAAIG
HHCCEEEEECCCCCCEEEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCHHHHH
IVTLGILVFWPRLGIRLPGHLPALLAGCAVMGIVNLLGGHVATIGSQFHYVLADGSQGNG
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHCCCEEEEEECCCCCCC
IPQLLPQLVLPWDLPNSEFTLTWDSIRTLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHK
HHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
ANSELVGQGLGNIIAPFFGGITATAAIARSAANVRAGATSPISAVIHSILVILALLVLAP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
LLSWLPLSAMAALLLMVAWNMSEAHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISV
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
GIVLASLLFMRRIARMTRLAPVVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRI
HHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
VILKWDAVPVLDAGGLDAFQRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFF
EEEEECCCCEECCCCHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHCCCCCCCCCEEEC
PNRRAAMADL
CCCCCCCCCH
>Mature Secondary Structure 
PFRALIDACWKEKYTAARFTRDLIAGITVGIIAIPLAMALAIGSGVAPQYGLYTAAVAG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
IVIALTGGSRFSVSGPTAAFVVILYPVSQQFGLAGLLVATLLSGIFLILMGLARFGRLIE
HEEEEECCCEEECCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YIPVSVTLGFTSGIGITIGTMQIKDFLGLQMAHVPEHYLQKVGALFMALPTINVGDAAIG
HHCCEEEEECCCCCCEEEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCHHHHH
IVTLGILVFWPRLGIRLPGHLPALLAGCAVMGIVNLLGGHVATIGSQFHYVLADGSQGNG
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHCCCEEEEEECCCCCCC
IPQLLPQLVLPWDLPNSEFTLTWDSIRTLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHK
HHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
ANSELVGQGLGNIIAPFFGGITATAAIARSAANVRAGATSPISAVIHSILVILALLVLAP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
LLSWLPLSAMAALLLMVAWNMSEAHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISV
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
GIVLASLLFMRRIARMTRLAPVVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRI
HHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
VILKWDAVPVLDAGGLDAFQRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFF
EEEEECCCCEECCCCHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHCCCCCCCCCEEEC
PNRRAAMADL
CCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; SO42- [Periplasm] [C]

Specific reaction: Proton [Periplasm] + SO42- [Periplasm] = Proton [Cytoplasm] + SO42- [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796