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The map label for this gene is ndh

Identifier: 157160636

GI number: 157160636

Start: 1229773

End: 1231077

Strand: Direct

Name: ndh

Synonym: EcHS_A1232

Alternate gene names: 157160636

Gene position: 1229773-1231077 (Clockwise)

Preceding gene: 157160635

Following gene: 157160637

Centisome position: 26.48

GC content: 52.72

Gene sequence:

>1305_bases
TTGACTACGCCATTGAAAAAAATTGTGATTGTCGGCGGCGGTGCTGGTGGGCTGGAAATGGCAACACAGCTGGGGCATAA
GCTGGGACGCAAGAAAAAAGCCAAAATTACGCTGGTCGATCGTAACCACAGCCACCTGTGGAAACCGCTGCTGCACGAAG
TGGCGACTGGCTCGCTTGATGAAGGCGTCGATGCGTTGAGCTATCTGGCCCATGCGCGCAATCATGGTTTCCAGTTCCAG
CTGGGTTCCGTCATTGATATTGATCGTGAAGCGAAAACAATCACTATTGCAGAACTGCGCGATGAGAAAGGTGAACTGCT
GGTTCCGGAACGTAAAATCGCCTATGACACCCTGGTAATGGCGCTGGGTAGCACCTCTAACGATTTCAATACGCCGGGTG
TCAAAGAGAACTGCATTTTCCTCGATAACCCGCACCAGGCGCGTCGCTTTCACCAGGAGATGCTGAATCTCTTCCTGAAA
TACTCCGCCAACCTGGGCGCGAATGGCAAAGTGAACATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGC
TGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGG
TAGAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGC
GTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAGGCGGCCTGCACACTAAAGATGGCGAATATATTGAGGC
GGATCTGATGGTATGGGCAGCCGGGATCAAAGCGCCAGACTTCCTGAAAGATATCGGTGGTCTTGAAACTAACCGTATCA
ACCAGCTGGTGGTGGAACCGACGCTGCAAACCACTCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCG
CGTCCGGAAGGGGGCTTTGTTCCGCCGCGTGCTCAGGCTGCACACCAGATGGCGACTTGCGCAATGAACAACATTCTGGC
GCAGATGAACGGTAAACCGCTGAAAAATTATCAGTATAAAGATCATGGTTCGCTGGTATCGCTGTCGAACTTCTCCACCG
TTGGTAGCCTGATGGGTAACCTGACGCGCGGCTCAATGATGATTGAAGGACGAATTGCGCGCTTTGTATATATCTCGCTA
TACCGAATGCATCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGAGTATTAACCGCGTTAT
CCGTCCGCGTTTGAAGTTGCATTAA

Upstream 100 bases:

>100_bases
AATAGCGAAGAACATTTTCATTGCTGTAACCTGTTGTTAATTAAGAGCTATGTTAATAACCATTAATTAACAATTGGTTA
ATAAATTTAAGGGGGTCACG

Downstream 100 bases:

>100_bases
TCGACGTACACTGGCGGATGTGGCATAAACGCCTCATCCGCCCTGGACAAATGGTTCGAGCGGTAGGCCTGATAAGACGC
GTTAAGCGTCGCATCAGGCA

Product: NADH dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 434; Mature: 433

Protein sequence:

>434_residues
MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHSHLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQ
LGSVIDIDREAKTITIAELRDEKGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGERILPALPPRISAAAHNELTKLG
VRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGIKAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCP
RPEGGFVPPRAQAAHQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRIARFVYISL
YRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH

Sequences:

>Translated_434_residues
MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHSHLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQ
LGSVIDIDREAKTITIAELRDEKGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGERILPALPPRISAAAHNELTKLG
VRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGIKAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCP
RPEGGFVPPRAQAAHQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRIARFVYISL
YRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH
>Mature_433_residues
TTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHSHLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQL
GSVIDIDREAKTITIAELRDEKGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLKY
SANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGERILPALPPRISAAAHNELTKLGV
RVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGIKAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPR
PEGGFVPPRAQAAHQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRIARFVYISLY
RMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH

Specific function: Transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Does not couple the redox reaction to proton translocation

COG id: COG1252

COG function: function code C; NADH dehydrogenase, FAD-containing subunit

Gene ontology:

Cell location: Cell membrane

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH dehydrogenase family

Homologues:

Organism=Escherichia coli, GI1787352, Length=434, Percent_Identity=100, Blast_Score=896, Evalue=0.0,
Organism=Escherichia coli, GI1789065, Length=358, Percent_Identity=26.536312849162, Blast_Score=71, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6320118, Length=349, Percent_Identity=26.3610315186246, Blast_Score=83, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6323515, Length=464, Percent_Identity=26.0775862068966, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6323794, Length=349, Percent_Identity=28.3667621776504, Blast_Score=79, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DHNA_ECOLI (P00393)

Other databases:

- EMBL:   V00306
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   A00461
- RefSeq:   AP_001735.1
- RefSeq:   NP_415627.1
- PDB:   1OZK
- PDBsum:   1OZK
- ProteinModelPortal:   P00393
- SMR:   P00393
- DIP:   DIP-10325N
- STRING:   P00393
- PeroxiBase:   5949
- EnsemblBacteria:   EBESCT00000002548
- EnsemblBacteria:   EBESCT00000014552
- GeneID:   946792
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW1095
- KEGG:   eco:b1109
- EchoBASE:   EB0643
- EcoGene:   EG10649
- eggNOG:   COG1252
- GeneTree:   EBGT00050000009316
- HOGENOM:   HBG668030
- OMA:   HRFRTEM
- ProtClustDB:   CLSK874586
- BioCyc:   EcoCyc:NADH-DHII-MONOMER
- BioCyc:   MetaCyc:NADH-DHII-MONOMER
- Genevestigator:   P00393
- GO:   GO:0006810
- InterPro:   IPR013027
- InterPro:   IPR001327
- PRINTS:   PR00368

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2

EC number: =1.6.99.3

Molecular weight: Translated: 47359; Mature: 47228

Theoretical pI: Translated: 9.19; Mature: 9.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHSHLWKPLLHEVATGSLD
CCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHCCCHH
EGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDEKGELLVPERKIAYDTLVM
HHHHHHHHHHHHHCCCCEEECCCEEECCCCCCEEEEEEECCCCCCEECCCHHHHHHHHHH
ALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLKYSANLGANGKVNIAIVGGGA
HHCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCC
TGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGERILPALPPRISAAAHNELTKLG
CCEEEHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHCCCCCCCCCHHHHHHHHHHH
VRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGIKAPDFLKDIGGLETNRINQLVVEP
HHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHCCCCCCCCCEEEECC
TLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAAHQMATCAMNNILAQMNGKPLKNYQYK
CHHCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
DHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRIARFVYISLYRMHQIALHGYFKTGLMMLV
CCCCEEEECCHHHHHHHHHCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GSINRVIRPRLKLH
HHHHHHHCCHHCCC
>Mature Secondary Structure 
TTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHSHLWKPLLHEVATGSLD
CCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHCCCHH
EGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDEKGELLVPERKIAYDTLVM
HHHHHHHHHHHHHCCCCEEECCCEEECCCCCCEEEEEEECCCCCCEECCCHHHHHHHHHH
ALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLKYSANLGANGKVNIAIVGGGA
HHCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCC
TGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGERILPALPPRISAAAHNELTKLG
CCEEEHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHCCCCCCCCCHHHHHHHHHHH
VRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGIKAPDFLKDIGGLETNRINQLVVEP
HHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHCCCCCCCCCEEEECC
TLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAAHQMATCAMNNILAQMNGKPLKNYQYK
CHHCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
DHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRIARFVYISLYRMHQIALHGYFKTGLMMLV
CCCCEEEECCHHHHHHHHHCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GSINRVIRPRLKLH
HHHHHHHCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 6265208; 8905232; 9278503