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The map label for this gene is mdoH

Identifier: 157160575

GI number: 157160575

Start: 1174536

End: 1177079

Strand: Direct

Name: mdoH

Synonym: EcHS_A1171

Alternate gene names: 157160575

Gene position: 1174536-1177079 (Clockwise)

Preceding gene: 157160574

Following gene: 157160576

Centisome position: 25.29

GC content: 54.76

Gene sequence:

>2544_bases
ATGAATAAGACAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCATTGCCGAAGACTGATATCCG
CGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGGCTCGGTAAAGGCGC
GTCTGGAACAAGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCG
ATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAACCCGGTAGGCCGTTTCTGGGATCGCCTGCG
TGGACGCGATGTCACGCCGCGCTATCTGGCTCGTTTGACCAAAGAAGAGCAGGAGAGCGAGCAAAAGTGGCGTACCGTCG
GTACCATCCGCCGTTACATTCTGTTGATCCTGACGCTCGCGCAAACTGTCGTCGCGACCTGGTATATGAAGACCATTCTT
CCTTATCAGGGTTGGGCGCTGATTAATCCTATGGATATGGTTGGTCAGGATTTGTGGGTTTCCTTTATGCAGCTTCTGCC
TTATATGCTGCAAACCGGTATCCTGATCCTCTTTGCGGTACTGTTCTGTTGGGTGTCCGCCGGATTCTGGACGGCGTTAA
TGGGCTTCCTGCAACTGCTTATTGGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCG
GAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGA
ATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCGTCG
CAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGC
GTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGC
TGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGT
CGTCGCCGAAAGCGTCCGGTATGGATACGCTGTATGCGCGCTGTCAGCAGTTCGCGACCCGCGTGTATGGGCCACTGTTT
ACAGCCGGTTTGCACTTCTGGCAACTTGGCGAGTCGCACTACTGGGGACATAACGCGATTATCCGCGTGAAACCGTTTAT
CGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGACTTCGTGGAAGCGG
CGTTGATGCGCCGTGCAGGTTGGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTG
CTTGATGAGCTAAAACGTGACCGCCGCTGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCTTGGTGAAGGGTATGCA
CCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTCTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCT
CTACTGCATTGCAGGTAGTACATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCGGTGTGGCCG
CAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACTATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTT
GCTTATCTGGTGCAAAGGAACGAAAGAATACGGCGGATTCTGGCGCGTTACATTATCGTTGCTGCTGGAAGTGCTGTTTT
CCGTGCTGCTGGCTCCGGTACGCATGCTGTTCCATACGGTCTTCGTCGTCAGCGCGTTCCTTGGCTGGGAAGTTGTGTGG
AATTCACCGCAGCGTGATGATGACTCCACTTCCTGGGGTGAAGCGTTCAAACGCCACGGCTCACAGCTGCTGTTAGGGTT
AGTGTGGGCTGTTGGTATGGCGTGGTTGGATCTGCGTTTCCTGTTCTGGCTGGCACCGATTGTCTTCTCGTTGATCCTGT
CACCGTTTGTTTCGGTGATTTCCAGCCGTGCCACCGTTGGTCTGCGAACCAAACGCTGGAAACTGTTCCTGATCCCGGAA
GAGTATTCACCGCCGCAGGTGCTGGTTGATACCGATCGCTTCCTTGAGATGAATCGTCAACGCTCCCTTGATGATGGTTT
TATGCACGCGGTGTTTAACCCGTCATTTAACGCTCTGGCAACCGCAATGGCGACCGCGCGTCACCGCGCCAGTAAGGTGC
TGGAAATCGCCCGTGACCGCCACGTTGAACAGGCGCTTAACGAGACGCCAGAGAAGCTGAATCGCGATCGTCGCCTGGTG
CTGCTAAGCGATCCGGTGACGATGGCCCGTCTGCATTTCCGCGTCTGGAATTCCCCGGAGAGATATTCTTCATGGGTGAG
TTATTACGAAGGGATAAAGCTCAATCCACTGGCATTGCGTAAACCGGATGCGGCTTCGCAATAA

Upstream 100 bases:

>100_bases
GTGAAAGTGAAAGATGCCAAGAAAACCACTGAAATGCGTGCTGCGCTGGTGAATGCCGATCAGACGTTGAGTGAAACCTG
GAGCTACCAGTTACCTGCCA

Downstream 100 bases:

>100_bases
AAACGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGATTTTGTAGGT
TGGGTAAGGCGTTCACGCCG

Product: glucosyltransferase MdoH

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 847; Mature: 847

Protein sequence:

>847_residues
MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWAREDDSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKA
MPEAKRSSMFPDPWRTNPVGRFWDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQLLIGRDKYSISASTVGDEPLNP
EHRTALIMPICNEDVNRVFAGLRATWESVKATGNAKHFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRR
VKRKSGNIDDFCRRWGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFATRVYGPLF
TAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDFVEAALMRRAGWGVWIAYDLPGSYEELPPNL
LDELKRDRRWCHGNLMNFRLFLVKGMHPVHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWP
QWRPELAIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLFHTVFVVSAFLGWEVVW
NSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLFWLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPE
EYSPPQVLVDTDRFLEMNRQRSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRRLV
LLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ

Sequences:

>Translated_847_residues
MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWAREDDSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKA
MPEAKRSSMFPDPWRTNPVGRFWDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQLLIGRDKYSISASTVGDEPLNP
EHRTALIMPICNEDVNRVFAGLRATWESVKATGNAKHFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRR
VKRKSGNIDDFCRRWGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFATRVYGPLF
TAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDFVEAALMRRAGWGVWIAYDLPGSYEELPPNL
LDELKRDRRWCHGNLMNFRLFLVKGMHPVHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWP
QWRPELAIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLFHTVFVVSAFLGWEVVW
NSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLFWLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPE
EYSPPQVLVDTDRFLEMNRQRSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRRLV
LLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ
>Mature_847_residues
MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWAREDDSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKA
MPEAKRSSMFPDPWRTNPVGRFWDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQLLIGRDKYSISASTVGDEPLNP
EHRTALIMPICNEDVNRVFAGLRATWESVKATGNAKHFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRR
VKRKSGNIDDFCRRWGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFATRVYGPLF
TAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDFVEAALMRRAGWGVWIAYDLPGSYEELPPNL
LDELKRDRRWCHGNLMNFRLFLVKGMHPVHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWP
QWRPELAIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLFHTVFVVSAFLGWEVVW
NSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLFWLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPE
EYSPPQVLVDTDRFLEMNRQRSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRRLV
LLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ

Specific function: Involved in the biosynthesis of osmoregulated periplasmic glucans (OPGs)

COG id: COG2943

COG function: function code M; Membrane glycosyltransferase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 2 family. OpgH subfamily

Homologues:

Organism=Escherichia coli, GI1787287, Length=847, Percent_Identity=100, Blast_Score=1744, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): OPGH_ECO57 (P62518)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- RefSeq:   NP_287183.1
- RefSeq:   NP_309454.1
- ProteinModelPortal:   P62518
- EnsemblBacteria:   EBESCT00000025315
- EnsemblBacteria:   EBESCT00000057024
- GeneID:   913619
- GeneID:   959381
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z1684
- KEGG:   ecs:ECs1427
- GeneTree:   EBGT00050000010387
- HOGENOM:   HBG668600
- OMA:   MSGECLT
- ProtClustDB:   PRK05454
- BioCyc:   ECOL83334:ECS1427-MONOMER
- HAMAP:   MF_01072
- InterPro:   IPR001173

Pfam domain/function: PF00535 Glycos_transf_2

EC number: 2.4.1.- [C]

Molecular weight: Translated: 96938; Mature: 96938

Theoretical pI: Translated: 8.84; Mature: 8.84

Prosite motif: PS00213 LIPOCALIN

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x13b9b0ec)-; HASH(0x13ba73b0)-; HASH(0x141117c0)-; HASH(0x13fb6030)-; HASH(0x13d4c6ac)-; HASH(0x135e76b4)-;

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWAREDDSPQGSVKARLEQAWP
CCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCHHHHHCCCCCCCCCCHHHHHHHHHCC
DSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRFWDRLRGRDVTPRYLARLT
CCCCCCCEECCCCCHHHHHHCCHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHH
KEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTILPYQGWALINPMDMVGQDLWV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECHHHHHHHHHHH
SFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQLLIGRDKYSISASTVGDEPLNP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCC
EHRTALIMPICNEDVNRVFAGLRATWESVKATGNAKHFDVYILSDSYNPDICVAEQKAWM
CCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCEEEECHHHHH
ELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRRWGSQYSYMVVLDADSVMTGDCLCGLV
HHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHH
RLMEANPNAGIIQSSPKASGMDTLYARCQQFATRVYGPLFTAGLHFWQLGESHYWGHNAI
HHHHCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEE
IRVKPFIEHCALAPLPGEGSFAGSILSHDFVEAALMRRAGWGVWIAYDLPGSYEELPPNL
EEEEHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHCCHHH
LDELKRDRRWCHGNLMNFRLFLVKGMHPVHRAVFLTGVMSYLSAPLWFMFLALSTALQVV
HHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HALTEPQYFLQPRQLFPVWPQWRPELAIALFASTMVLLFLPKLLSILLIWCKGTKEYGGF
HHHCCCHHHCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCH
WRVTLSLLLEVLFSVLLAPVRMLFHTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCHHHHHHHHHH
SQLLLGLVWAVGMAWLDLRFLFWLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECEEEEEECC
EYSPPQVLVDTDRFLEMNRQRSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDR
CCCCCEEEECCHHHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
HVEQALNETPEKLNRDRRLVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALR
HHHHHHHCCHHHHCCCCEEEEEECCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEECC
KPDAASQ
CCCCCCH
>Mature Secondary Structure
MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWAREDDSPQGSVKARLEQAWP
CCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCHHHHHCCCCCCCCCCHHHHHHHHHCC
DSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRFWDRLRGRDVTPRYLARLT
CCCCCCCEECCCCCHHHHHHCCHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHH
KEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTILPYQGWALINPMDMVGQDLWV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECHHHHHHHHHHH
SFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQLLIGRDKYSISASTVGDEPLNP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCC
EHRTALIMPICNEDVNRVFAGLRATWESVKATGNAKHFDVYILSDSYNPDICVAEQKAWM
CCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCEEEECHHHHH
ELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRRWGSQYSYMVVLDADSVMTGDCLCGLV
HHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHH
RLMEANPNAGIIQSSPKASGMDTLYARCQQFATRVYGPLFTAGLHFWQLGESHYWGHNAI
HHHHCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEE
IRVKPFIEHCALAPLPGEGSFAGSILSHDFVEAALMRRAGWGVWIAYDLPGSYEELPPNL
EEEEHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHCCHHH
LDELKRDRRWCHGNLMNFRLFLVKGMHPVHRAVFLTGVMSYLSAPLWFMFLALSTALQVV
HHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HALTEPQYFLQPRQLFPVWPQWRPELAIALFASTMVLLFLPKLLSILLIWCKGTKEYGGF
HHHCCCHHHCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCH
WRVTLSLLLEVLFSVLLAPVRMLFHTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCHHHHHHHHHH
SQLLLGLVWAVGMAWLDLRFLFWLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECEEEEEECC
EYSPPQVLVDTDRFLEMNRQRSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDR
CCCCCEEEECCHHHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
HVEQALNETPEKLNRDRRLVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALR
HHHHHHHCCHHHHCCCCEEEEEECCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEECC
KPDAASQ
CCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796