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The map label for this gene is ghrA [H]

Identifier: 157160555

GI number: 157160555

Start: 1160782

End: 1161720

Strand: Direct

Name: ghrA [H]

Synonym: EcHS_A1151

Alternate gene names: 157160555

Gene position: 1160782-1161720 (Clockwise)

Preceding gene: 157160553

Following gene: 157160556

Centisome position: 25.0

GC content: 51.12

Gene sequence:

>939_bases
ATGGATATCATCTTTTATCACCCAACGTTCGATACCCAATGGTGGATTGAGGCACTGCGCAAAGCTATTCCTCAGGCAAG
AGTCAGAGCATGGAAAAGCGGAGATAATGACTCTGCTGATTATGCTTTAGTCTGGCATCCTCCTGTTGAAATGCTGGCAG
GGCGCGATCTTAAAGCGGTGTTCGCACTCGGGGCCGGTGTTGATTCTATTTTGAGCAAGCTACAGGCACACCCTGAAATG
CTGAACCCTTCTGTTCCACTTTTTCGCCTGGAAGATACCGGTATGGGCGAGCAAATGCAGGAATATGCTGTCAGTCAGGT
GCTGCATTGGTTTCGACGTTTTGACGATTATCGCATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAATATCATC
GGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTT
CCGCTGCGTTGCTGGAGTCGAACCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGACGGGAAGAACTGTCTGCATT
TCTGAGCCAATGTCGGGTATTGATTAATTTGTTACCGAATACCCCTGAAACCGTCGGCATTATTAATCAACAATTACTCG
AAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTG
GATAGCGGGAAAGTTAAAGGCGCAATGCTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACA
TCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTGGAGTACATTTCTCGCACTATTG
CCCAGCTCGAAAAAGGGGAGAAGGTCTGCGGGCAAGTCGACCGCGCACGCGGCTACTAA

Upstream 100 bases:

>100_bases
CTTTTTTTGCCACCGACCACGGATTTGTTATGCTGGTGGCTTTTGTAGATCATAACGATAAGTGCGAATAAATTTCGCAC
AACGCTTTTCGGGAGTCAGT

Downstream 100 bases:

>100_bases
TAAAGCATCAGGATTCCTGCTATCCTTGGCGGGAATTGAATACAGGAGAGAGTTATGTATCCCGTCGACCTTCATATGCA
TACCGTTGCCAGCACACATG

Product: D-isomer specific 2-hydroxyacid dehydrogenase family protein

Products: NA

Alternate protein names: 2-ketoacid reductase [H]

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALVWHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEM
LNPSVPLFRLEDTGMGEQMQEYAVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPDGAYLLNLARGVHVVEDDLLAAL
DSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHVAAITRPAEAVEYISRTIAQLEKGEKVCGQVDRARGY

Sequences:

>Translated_312_residues
MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALVWHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEM
LNPSVPLFRLEDTGMGEQMQEYAVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPDGAYLLNLARGVHVVEDDLLAAL
DSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHVAAITRPAEAVEYISRTIAQLEKGEKVCGQVDRARGY
>Mature_312_residues
MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALVWHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEM
LNPSVPLFRLEDTGMGEQMQEYAVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPDGAYLLNLARGVHVVEDDLLAAL
DSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHVAAITRPAEAVEYISRTIAQLEKGEKVCGQVDRARGY

Specific function: Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively [H]

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrA subfamily [H]

Homologues:

Organism=Homo sapiens, GI6912396, Length=237, Percent_Identity=28.2700421940928, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI23308577, Length=261, Percent_Identity=27.2030651340996, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI87081824, Length=312, Percent_Identity=99.6794871794872, Blast_Score=643, Evalue=0.0,
Organism=Escherichia coli, GI87082289, Length=284, Percent_Identity=27.4647887323944, Blast_Score=75, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17532191, Length=268, Percent_Identity=28.3582089552239, Blast_Score=75, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6324964, Length=214, Percent_Identity=25.7009345794392, Blast_Score=72, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6324980, Length=178, Percent_Identity=28.0898876404494, Blast_Score=70, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6324055, Length=179, Percent_Identity=29.0502793296089, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI28574286, Length=262, Percent_Identity=29.7709923664122, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24585514, Length=243, Percent_Identity=26.3374485596708, Blast_Score=88, Evalue=6e-18,
Organism=Drosophila melanogaster, GI28574282, Length=243, Percent_Identity=26.3374485596708, Blast_Score=88, Evalue=6e-18,
Organism=Drosophila melanogaster, GI45552429, Length=243, Percent_Identity=26.3374485596708, Blast_Score=88, Evalue=7e-18,
Organism=Drosophila melanogaster, GI45551003, Length=243, Percent_Identity=26.3374485596708, Blast_Score=88, Evalue=8e-18,
Organism=Drosophila melanogaster, GI28574284, Length=243, Percent_Identity=26.3374485596708, Blast_Score=88, Evalue=9e-18,
Organism=Drosophila melanogaster, GI24585516, Length=234, Percent_Identity=27.3504273504274, Blast_Score=74, Evalue=9e-14,
Organism=Drosophila melanogaster, GI19921140, Length=170, Percent_Identity=31.7647058823529, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI28571528, Length=173, Percent_Identity=28.9017341040462, Blast_Score=66, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.79; =1.1.1.81 [H]

Molecular weight: Translated: 35316; Mature: 35316

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALVWHPPVEMLAGRDLKAV
CCEEEECCCCCHHHHHHHHHHHCCHHHHHHHCCCCCCCCCEEEEECCCHHHHCCCCHHHH
FALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEYAVSQVLHWFRRFDDYRIQ
HHHHCCHHHHHHHHHHCHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCHHCC
QNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRFPLRCWSRTRKSWPGVQSFAG
CCCCCCCCCHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCC
REELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPDGAYLLNLARGVHVVEDDLLAAL
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHH
DSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHVAAITRPAEAVEYISRTIAQLEKGE
HCCCCCHHHHHHHCCCCCCCCCCCCCCCCEEECCCHHHHCCHHHHHHHHHHHHHHHHHHH
KVCGQVDRARGY
HHHHHHHHHCCC
>Mature Secondary Structure
MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALVWHPPVEMLAGRDLKAV
CCEEEECCCCCHHHHHHHHHHHCCHHHHHHHCCCCCCCCCEEEEECCCHHHHCCCCHHHH
FALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEYAVSQVLHWFRRFDDYRIQ
HHHHCCHHHHHHHHHHCHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCHHCC
QNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRFPLRCWSRTRKSWPGVQSFAG
CCCCCCCCCHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCC
REELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPDGAYLLNLARGVHVVEDDLLAAL
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHH
DSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHVAAITRPAEAVEYISRTIAQLEKGE
HCCCCCHHHHHHHCCCCCCCCCCCCCCCCEEECCCHHHHCCHHHHHHHHHHHHHHHHHHH
KVCGQVDRARGY
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA