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The map label for this gene is rutB [H]

Identifier: 157160535

GI number: 157160535

Start: 1134469

End: 1135164

Strand: Reverse

Name: rutB [H]

Synonym: EcHS_A1126

Alternate gene names: 157160535

Gene position: 1135164-1134469 (Counterclockwise)

Preceding gene: 157160536

Following gene: 157160534

Centisome position: 24.45

GC content: 55.32

Gene sequence:

>696_bases
ATGATGACGACATTAACCGCTCGACCGGAAGCCATTACCTTCGATCCGCAGCAAAGTGCGCTGATCGTGGTGGATATGCA
AAACGCCTATGCCACGCCAGGCGGCTACTTAGATCTCGCCGGGTTTGATGTCTCAACCACTCGCCCGGTCATTGCCAACA
TTCAAACCGCCGTGACTGCAGCGCGAGCGGCAGGGATGTTGATCATCTGGTTTCAAAATGGCTGGGATGAACAGTATGTC
GAGGCTGGCGGCCCTGGCTCACCGAATTTTCATAAATCGAACGCCCTGAAAACCATGCGTAAACAGCCGCAGCTGCAGGG
GAAATTGCTGGCGAAAGGCTCCTGGGATTATCAACTGGTGGATGAACTGGTGCCGCAGCCTGGCGATATTGTGCTGCCGA
AGCCGCGCTACAGCGGTTTCTTCAATACGCCGCTGGACAGCATTTTGCGCAGCCGCGGAATACGCCATCTGGTTTTCACC
GGCATCGCTACCAACGTCTGCGTCGAATCGACGCTACGCGACGGCTTTTTTCTGGAGTATTTCGGCGTGGTGCTGGAAGA
CGCAACTCACCAGGCGGGGCCGGAATTTGCGCAGAAAGCTGCGTTGTTCAATATCGAAACCTTTTTTGGCTGGGTCAGCG
ACGTCGAAACGTTCTGCGACGCGCTTTCTCCCACGTCCTTTGCTCGTATCGCTTAA

Upstream 100 bases:

>100_bases
CCTTCGACGATTTTCTGTCGGGAATCGAAACCTTCGGCGAGCGCATTCAACCACTGATGCAGTGCCGCGCCCATCTCCCT
GTGCTGACTCAGGAGGTGGC

Downstream 100 bases:

>100_bases
GGAGTTTAACGATGCCAAAATCCGTAATTATTCCCGCTGGCAGCAGCGCACCGCTGGCCCCCTTCGTTCCCGGCACGCTG
GCTGATGGAGTGGTGTATGT

Product: putative isochorismatase family protein, rutB

Products: NA

Alternate protein names: Ureidoacrylate amidohydrolase [H]

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MMTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVSTTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYV
EAGGPGSPNFHKSNALKTMRKQPQLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFT
GIATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFCDALSPTSFARIA

Sequences:

>Translated_231_residues
MMTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVSTTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYV
EAGGPGSPNFHKSNALKTMRKQPQLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFT
GIATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFCDALSPTSFARIA
>Mature_231_residues
MMTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVSTTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYV
EAGGPGSPNFHKSNALKTMRKQPQLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFT
GIATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFCDALSPTSFARIA

Specific function: In vivo, quickly hydrolyzes the ureidoacrylate peracid to avoid toxicity, but can also hydrolyzes ureidoacrylate that is formed spontaneously from ureidoacrylate peracid. One of the products of hydrolysis, carbamate, hydrolyzes spontaneously, thereby rele

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismatase family. RutB subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081820, Length=230, Percent_Identity=99.1304347826087, Blast_Score=472, Evalue=1e-135,
Organism=Escherichia coli, GI87081992, Length=211, Percent_Identity=28.436018957346, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019916
- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: NA

Molecular weight: Translated: 25360; Mature: 25360

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVSTTRPVIANIQTAVTA
CCCCCCCCCCEEEECCCCCEEEEEECCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHH
ARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQPQLQGKLLAKGSWDYQLV
HHHCCEEEEEEECCCCHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCHHHH
DELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTGIATNVCVESTLRDGFFLEY
HHHCCCCCCEEECCCCCCCCCCCCHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCCHHHHH
FGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFCDALSPTSFARIA
HHHHHHHCCCCCCHHHHHHHHHEEHHHHHHHHHHHHHHHHHCCCCCHHHCC
>Mature Secondary Structure
MMTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVSTTRPVIANIQTAVTA
CCCCCCCCCCEEEECCCCCEEEEEECCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHH
ARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQPQLQGKLLAKGSWDYQLV
HHHCCEEEEEEECCCCHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCHHHH
DELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTGIATNVCVESTLRDGFFLEY
HHHCCCCCCEEECCCCCCCCCCCCHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCCHHHHH
FGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFCDALSPTSFARIA
HHHHHHHCCCCCCHHHHHHHHHEEHHHHHHHHHHHHHHHHHCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA