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The map label for this gene is cspH

Identifier: 157160507

GI number: 157160507

Start: 1111800

End: 1112012

Strand: Reverse

Name: cspH

Synonym: EcHS_A1098

Alternate gene names: 157160507

Gene position: 1112012-1111800 (Counterclockwise)

Preceding gene: 157160510

Following gene: 157160506

Centisome position: 23.95

GC content: 46.95

Gene sequence:

>213_bases
TTGTCCCGTAAAATGACAGGAATTGTCAAAACCTTTGATCGCAAAAGCGGCAAAGGATTCATTATCCCCTCCGACGGTCG
TAAAGAAGTTCAGGTCCATATTTCCGCATTCACTCCCCGCGACGCAGAAGTGCTTATACCAGGATTACGTGTGGAATTTT
GCCGCGTAAATGGCCTGCGAGGACCAACAGCGGCAAATGTTTATCTCTCTTAA

Upstream 100 bases:

>100_bases
AAGCAAATTTTATTTTAGCCGTCCGGCGGTCTTAAATATCGCTGGCTTTATTTTATTAACCCCGTATAGTGCAGCGCAGT
ATTTTCAGTAAGGAATTTGT

Downstream 100 bases:

>100_bases
TATGACGCTGCTTGTTTAAAGCTGACTGGTTTTATTGGAATAGTGCAATAGTCAACATCATACATTATAACATCAGTTAC
TTTTCCACAAAGAAACTAAT

Product: cold shock DNA-binding protein

Products: NA

Alternate protein names: CSP-H

Number of amino acids: Translated: 70; Mature: 69

Protein sequence:

>70_residues
MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEVLIPGLRVEFCRVNGLRGPTAANVYLS

Sequences:

>Translated_70_residues
MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEVLIPGLRVEFCRVNGLRGPTAANVYLS
>Mature_69_residues
SRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEVLIPGLRVEFCRVNGLRGPTAANVYLS

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain

Homologues:

Organism=Escherichia coli, GI2367112, Length=70, Percent_Identity=100, Blast_Score=144, Evalue=7e-37,
Organism=Escherichia coli, GI1787840, Length=70, Percent_Identity=77.1428571428572, Blast_Score=115, Evalue=5e-28,
Organism=Escherichia coli, GI2367114, Length=67, Percent_Identity=49.2537313432836, Blast_Score=65, Evalue=6e-13,
Organism=Escherichia coli, GI1787839, Length=70, Percent_Identity=47.1428571428571, Blast_Score=65, Evalue=6e-13,
Organism=Escherichia coli, GI1787834, Length=67, Percent_Identity=49.2537313432836, Blast_Score=65, Evalue=1e-12,
Organism=Escherichia coli, GI1789979, Length=67, Percent_Identity=49.2537313432836, Blast_Score=63, Evalue=4e-12,
Organism=Escherichia coli, GI1786841, Length=64, Percent_Identity=50, Blast_Score=62, Evalue=5e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): CSPH_ECO57 (P0A984)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   D85634
- PIR:   H90771
- RefSeq:   NP_286925.1
- RefSeq:   NP_309171.1
- ProteinModelPortal:   P0A984
- SMR:   P0A984
- EnsemblBacteria:   EBESCT00000028697
- EnsemblBacteria:   EBESCT00000060019
- GeneID:   912600
- GeneID:   959052
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z1405
- KEGG:   ecs:ECs1144
- GeneTree:   EBGT00050000010463
- HOGENOM:   HBG717773
- OMA:   VHISAFS
- ProtClustDB:   PRK15464
- BioCyc:   ECOL83334:ECS1144-MONOMER
- GO:   GO:0005737
- GO:   GO:0006350
- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF002599
- SMART:   SM00357

Pfam domain/function: PF00313 CSD; SSF50249 Nucleic_acid_OB

EC number: NA

Molecular weight: Translated: 7720; Mature: 7589

Theoretical pI: Translated: 10.95; Mature: 10.95

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEVLIPGLRVEFCRVNGLR
CCCCCCCEEEECCCCCCCEEEEECCCCEEEEEEEEEECCCCCEEEECCCEEEEEEECCCC
GPTAANVYLS
CCCEEEEEEC
>Mature Secondary Structure 
SRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEVLIPGLRVEFCRVNGLR
CCCCCCEEEECCCCCCCEEEEECCCCEEEEEEEEEECCCCCEEEECCCEEEEEEECCCC
GPTAANVYLS
CCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796