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The map label for this gene is ycaL [H]

Identifier: 157160431

GI number: 157160431

Start: 1020607

End: 1021371

Strand: Direct

Name: ycaL [H]

Synonym: EcHS_A1015

Alternate gene names: 157160431

Gene position: 1020607-1021371 (Clockwise)

Preceding gene: 157160430

Following gene: 157160432

Centisome position: 21.98

GC content: 49.8

Gene sequence:

>765_bases
ATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTGCAGCATTACTGACAGGGTGTCAAAATACCCACGGTATTGA
TACCAATATGGCTATCAGCTCCGGTTTAAATGCCTATAAAGCAGCAACATTAAGCGATGCCGATGCAAAAGCGATTGCCA
ATCAGGGCTGTGCCGAAATGGACAGCGGCAATCAAGTCGCAAGTAAATCCAGCAAGTACGGTAAACGTCTGGCAAAAATC
GCCAAAGCATTGGGTAACAATATTAATGGCACGCCGGTCAACTATAAGGTTTATATGACCAGCGACGTCAACGCATGGGC
GATGGCGAACGGCTGTGTTCGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTCTGGGCC
ATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTATGCGATCGTTGCCGCACGCGATGCC
ATTTCAGCTACCAGCGGTGTGGCTTCCCAGCTTTCCCGCTCACAATTAGGTGATATCGCAGAAGGCGCTATCAATGCTAA
ATACTCCCGTGATAAAGAGTCCGAAGCAGATGATTTCTCCTTTGATCTGTTGAAGAAACGTGGCATCAGCACCCAGGGGC
TGGTTGGCAGCTTTGAAAAACTGGCTAGCCTGGATGGCGGTCGCACCCAGTCCATGTTTGACTCTCACCCACCATCAACA
GAGCGTGCGCAACACATCCGTGATCGTATCGCCTCTGGTAAGTAA

Upstream 100 bases:

>100_bases
TTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGA
TTAATCAACGGATGATTCAC

Downstream 100 bases:

>100_bases
ATCATTGTCATCTTTCGGGCTGGTCTTCTGCCAGCCCGCTATAATTGCGCAATAAATCCCCATCTGAATACAGACAAAAC
TGGTTTTTGCACACAACGTT

Product: M48B family peptidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATLSDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKI
AKALGNNINGTPVNYKVYMTSDVNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSFEKLASLDGGRTQSMFDSHPPST
ERAQHIRDRIASGK

Sequences:

>Translated_254_residues
MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATLSDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKI
AKALGNNINGTPVNYKVYMTSDVNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSFEKLASLDGGRTQSMFDSHPPST
ERAQHIRDRIASGK
>Mature_254_residues
MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATLSDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKI
AKALGNNINGTPVNYKVYMTSDVNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSFEKLASLDGGRTQSMFDSHPPST
ERAQHIRDRIASGK

Specific function: Unknown

COG id: COG0501

COG function: function code O; Zn-dependent protease with chaperone function

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M48B family [H]

Homologues:

Organism=Escherichia coli, GI87081800, Length=254, Percent_Identity=99.6062992125984, Blast_Score=517, Evalue=1e-148,
Organism=Escherichia coli, GI87082185, Length=252, Percent_Identity=56.7460317460317, Blast_Score=297, Evalue=5e-82,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001915 [H]

Pfam domain/function: PF01435 Peptidase_M48 [H]

EC number: 3.4.24.- [C]

Molecular weight: Translated: 26767; Mature: 26767

Theoretical pI: Translated: 8.08; Mature: 8.08

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATLSDADAKAIANQGCAEM
CCCCEEEEEEHHHHHHHHCCCCCCCCCCCHHHHHCCCHHHHHCCCCHHHHHHHHCCCHHC
DSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSDVNAWAMANGCVRVYSGLM
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHH
DMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDAISATSGVASQLSRSQLGDIA
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHH
EGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSFEKLASLDGGRTQSMFDSHPPST
HHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHCCCCCCH
ERAQHIRDRIASGK
HHHHHHHHHHCCCC
>Mature Secondary Structure
MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATLSDADAKAIANQGCAEM
CCCCEEEEEEHHHHHHHHCCCCCCCCCCCHHHHHCCCHHHHHCCCCHHHHHHHHCCCHHC
DSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSDVNAWAMANGCVRVYSGLM
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHH
DMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDAISATSGVASQLSRSQLGDIA
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHH
EGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSFEKLASLDGGRTQSMFDSHPPST
HHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHCCCCCCH
ERAQHIRDRIASGK
HHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Zn [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 7836281 [H]