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The map label for this gene is rumB [H]

Identifier: 157160381

GI number: 157160381

Start: 958818

End: 959945

Strand: Direct

Name: rumB [H]

Synonym: EcHS_A0962

Alternate gene names: 157160381

Gene position: 958818-959945 (Clockwise)

Preceding gene: 157160380

Following gene: 157160382

Centisome position: 20.65

GC content: 55.14

Gene sequence:

>1128_bases
ATGCAGTGCGCACTTTACGACGCGGGTCGCTGTCGTTCCTGTCAGTGGATAACGCAGCCGATTCCAGAGCAACTCTCCGC
TAAAACCGCCGATCTTAAAAATCTGCTCGCCGACTTTCCGGTTGAGGAATGGTGCGCGCCGGTGTCAGGCCCGGAACAAG
GGTTTCGTAATAAAGCCAAAATGGTGGTGAGTGGTAGCGTTGAAAAACCACTGCTCGGTATGCTGCATCGAGACGGCACA
CCGGAAGACCTTTGTGACTGCCCGCTTTATCCTGCCTCATTTGCGCCCGTTTTTGCGGCGCTAAAACCGTTTATCGCCCG
AGCGGGGTTAACGCCCTACAACGTGGCGCGTAAGCGTGGCGAACTGAAATACATTTTGCTGACTGAAAGCCAGAGCGATG
GCGGCATGATGCTGCGTTTTGTGTTGCGTTCCGAAACCAAACTGGCGCAACTGCGTAAGGCGCTACCGTGGTTACAGGAA
CAACTACCGCAGCTAAAAGTTATTACCGTCAATATTCAGCCGGTACATATGGCGATTATGGAAGGGGAGACGGAGATCTA
CCTGACCGAACAACAGGCACTGGCGGAGCGTTTTAACGATGTGCCGCTGTGGGTCCGTCCGCAAAGTTTCTTCCAGACTA
ATCCGGCGGTCGCCAGCCAGCTGTACGCTACCGCGCGCGACTGGGTACGACAGCTGCCGGTTAAACATATGTGGGATCTG
TTCTGTGGCGTGGGGGGCTTTGGTTTACACTGCGCGACGCCTGACATGCAGTTAACCGGGATCGAAATTGCGCCAGAGGC
CATTGCCTGTGCAAAGCAGTCAGCCGCTGAACTGGGCTTAACGCGTTTGCAATTTCAGGCGCTGGACTCCACTCAGTTTG
CCACTGCTCAGGGGGAGGTGCCGGAGCTGGTGCTGGTTAACCCGCCGCGCCGCGGCATTGGTAAACCGCTGTGTGATTAT
CTCTCAACGATGGCACCGCGTTTTATCATCTACTCCAGCTGTAACGCCCAAACCATGGCGAAAGATGTCCGAGAACTGCC
TGGTTACCGCATCGAACGGGTACAGCTTTTTGATATGTTCCCGCACACCGCGCACTATGAAGTGCTGACGCTGCTGGTGA
AGCAATAA

Upstream 100 bases:

>100_bases
CTCATCCTGATGCTGCTGTTCGTTCCCTCGACCAGTCGGCGGTTCTTCCAGTTGCAATAATGTGTATAATCGTCGCCCCT
GATGATGTGAAGGTCAATGT

Downstream 100 bases:

>100_bases
AAAAGCCGCAGGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGC
CTACAAAAGCGTGCAAATTC

Product: 23S rRNA methyluridine methyltransferase

Products: NA

Alternate protein names: 23S rRNA(M-5-U747)-methyltransferase [H]

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEWCAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGT
PEDLCDCPLYPASFAPVFAALKPFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWVRPQSFFQTNPAVASQLYATARDWVRQLPVKHMWDL
FCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAAELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDY
LSTMAPRFIIYSSCNAQTMAKDVRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ

Sequences:

>Translated_375_residues
MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEWCAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGT
PEDLCDCPLYPASFAPVFAALKPFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWVRPQSFFQTNPAVASQLYATARDWVRQLPVKHMWDL
FCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAAELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDY
LSTMAPRFIIYSSCNAQTMAKDVRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ
>Mature_375_residues
MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEWCAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGT
PEDLCDCPLYPASFAPVFAALKPFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWVRPQSFFQTNPAVASQLYATARDWVRQLPVKHMWDL
FCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAAELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDY
LSTMAPRFIIYSSCNAQTMAKDVRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ

Specific function: Catalyzes the formation of 5-methyl-uridine at position 747 (M-5-U747) in 23S rRNA [H]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family. RumB subfamily [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=207, Percent_Identity=25.6038647342995, Blast_Score=72, Evalue=9e-13,
Organism=Homo sapiens, GI51173878, Length=207, Percent_Identity=25.6038647342995, Blast_Score=72, Evalue=9e-13,
Organism=Escherichia coli, GI1787083, Length=375, Percent_Identity=97.8666666666667, Blast_Score=766, Evalue=0.0,
Organism=Escherichia coli, GI1789148, Length=376, Percent_Identity=27.9255319148936, Blast_Score=108, Evalue=7e-25,
Organism=Escherichia coli, GI1790403, Length=182, Percent_Identity=29.6703296703297, Blast_Score=73, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24666579, Length=361, Percent_Identity=24.0997229916898, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011825
- InterPro:   IPR010280 [H]

Pfam domain/function: PF05958 tRNA_U5-meth_tr [H]

EC number: =2.1.1.189 [H]

Molecular weight: Translated: 41943; Mature: 41943

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: PS01230 TRMA_1 ; PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEWCAPVSGPEQGFRNKAK
CCEECCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHCE
MVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALKPFIARAGLTPYNVARKRG
EEEECCCCCHHHHHHHCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCC
ELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQEQLPQLKVITVNIQPVHMAIM
CEEEEEEECCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEEEEECEEEEEEE
EGETEIYLTEQQALAERFNDVPLWVRPQSFFQTNPAVASQLYATARDWVRQLPVKHMWDL
CCCEEEEEEHHHHHHHHHCCCCEEECCHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHH
FCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAAELGLTRLQFQALDSTQFATAQGEV
HHCCCCCCEEECCCCCEEECEEECHHHHHHHHHHHHHHCHHHHHHHHCCCCCEEECCCCC
PELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSCNAQTMAKDVRELPGYRIERVQLFDMF
CEEEEECCCCCCCCHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHCCCCEEEEEEEECCC
PHTAHYEVLTLLVKQ
CCCHHHHHHHHHHCC
>Mature Secondary Structure
MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEWCAPVSGPEQGFRNKAK
CCEECCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHCE
MVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALKPFIARAGLTPYNVARKRG
EEEECCCCCHHHHHHHCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCC
ELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQEQLPQLKVITVNIQPVHMAIM
CEEEEEEECCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEEEEECEEEEEEE
EGETEIYLTEQQALAERFNDVPLWVRPQSFFQTNPAVASQLYATARDWVRQLPVKHMWDL
CCCEEEEEEHHHHHHHHHCCCCEEECCHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHH
FCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAAELGLTRLQFQALDSTQFATAQGEV
HHCCCCCCEEECCCCCEEECEEECHHHHHHHHHHHHHHCHHHHHHHHCCCCCEEECCCCC
PELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSCNAQTMAKDVRELPGYRIERVQLFDMF
CEEEEECCCCCCCCHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHCCCCEEEEEEEECCC
PHTAHYEVLTLLVKQ
CCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA