Click here to switch to the map view.

The map label for this gene is yliF [H]

Identifier: 157160313

GI number: 157160313

Start: 903217

End: 904545

Strand: Direct

Name: yliF [H]

Synonym: EcHS_A0892

Alternate gene names: 157160313

Gene position: 903217-904545 (Clockwise)

Preceding gene: 157160312

Following gene: 157160317

Centisome position: 19.45

GC content: 43.34

Gene sequence:

>1329_bases
ATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTA
CACTCAAATGGTAAAGGAACGGGTGTATGGCCTGAAACAGTCCGTTATTGATACTGCTTTTGCGGTGGCAAATATTGCTG
AATATCGGCGTAGCGTGGCAATTGATCTTATCAACACGCTAAATCCCACGGAGGAACAGCTGTTGGTTGGCTTACGCACA
GCTTACGCTGACTCGGTTTCACCCTCTTACTTGTACGATGTCGGTCCTTATCTGATTTCCAGTGACGAATGTATTCAGGT
AAAGGAGTTCGAGAAAAATTATTGTGCAGATATTATGCAGGTTGTGAAGTATCGACATGTCAAAAATACAGGGTTTATCT
CTTTTGACGGTAAAACCTTCGTCTATTACCTCTATCCGGTAACTCACAATCGTAGTCTGATATTTTTGCTTGGTCTGGAG
CGTTTTTCTTTACTGTCAAAATCTCTGGCGATGGATAGCGAGAACCTGATGTTCTCTCTGTTTAAGAACGGTAAACCGGT
GACCGGTGATGAATTTAATGCTAAAAACACCATTTTCACCGTTTCGGAAGCGATGGAGCACTTTGCCTATTTGCCGACCG
GATTGTACGTATTTGCGTATAAAAAAGATGTTTATTTGCGGGTTTGTACATTGATCATTTTCTTTGCCGCATTGGTGGCA
GTGATATCGGGTGCCAGTTGCCTCTATCTGGTACGCAGAGTGATTAATCGTGGTATTGTGGAGAAAGAAGCCATTATTAA
TAACCATTTTGAACGCGTACTGGATGGCGGGCTTTTCTTTTCGGCTGCCGATGTCAAAAAACTCTACAGTATGTATAACT
CGGCGTTCCTGGACGACCTGACCAAAGCAATGGGCAGAAAATCCTTTGACGAAGATTTAAAAGCGCTGCCGGAAAAAGGC
GGTTATTTGTGCCTGTTTGACGTCGATAAATTCAAAAATATTAACGACACCTTCGGTCATTTGCTGGGCGATGAAGTGTT
GATGAAAGTGGTGAAAATCCTTAAATCACAGATCCCGGTAGATAAAGGTAAAGTCTACCGCTTCGGCGGTGACGAATTTG
CGGTGATTTATACTGGCGGCACGCTGGAAGAGTTGCTATCGATACTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATT
AATTTAAGCACCAGTATCGGTGTAGCGCATTCAAATGAATGTACTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCG
ACTGTATAAGAGTAAGAAAAACGGCAGGGCTCAGATTAGCTGGCAGTAG

Upstream 100 bases:

>100_bases
AAATTAGGATGTGTTCAGGCTCAGGGGTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATT
AGTAAAAGAGTAGTTTACGT

Downstream 100 bases:

>100_bases
AATTAGAATAGTGTTAGTACCCCTGAATCAAGGGGTACTAATGAATGAGGTAAATCATTTGCAAACATTTTACTTTGCAA
AAACTTCCGGCCCTTTTATT

Product: diguanylate cyclase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 442; Mature: 441

Protein sequence:

>442_residues
MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYGLKQSVIDTAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRT
AYADSVSPSYLYDVGPYLISSDECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEFNAKNTIFTVSEAMEHFAYLPTGLYVFAYKKDVYLRVCTLIIFFAALVA
VISGASCLYLVRRVINRGIVEKEAIINNHFERVLDGGLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKG
GYLCLFDVDKFKNINDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEIVHFQVGSI
NLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ

Sequences:

>Translated_442_residues
MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYGLKQSVIDTAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRT
AYADSVSPSYLYDVGPYLISSDECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEFNAKNTIFTVSEAMEHFAYLPTGLYVFAYKKDVYLRVCTLIIFFAALVA
VISGASCLYLVRRVINRGIVEKEAIINNHFERVLDGGLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKG
GYLCLFDVDKFKNINDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEIVHFQVGSI
NLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ
>Mature_441_residues
SRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYGLKQSVIDTAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTA
YADSVSPSYLYDVGPYLISSDECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLER
FSLLSKSLAMDSENLMFSLFKNGKPVTGDEFNAKNTIFTVSEAMEHFAYLPTGLYVFAYKKDVYLRVCTLIIFFAALVAV
ISGASCLYLVRRVINRGIVEKEAIINNHFERVLDGGLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGG
YLCLFDVDKFKNINDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEIVHFQVGSIN
LSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787056, Length=442, Percent_Identity=99.0950226244344, Blast_Score=896, Evalue=0.0,
Organism=Escherichia coli, GI1787816, Length=191, Percent_Identity=32.4607329842932, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI145693134, Length=162, Percent_Identity=35.1851851851852, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI87081881, Length=163, Percent_Identity=32.5153374233129, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1788956, Length=156, Percent_Identity=32.0512820512821, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI87082007, Length=171, Percent_Identity=32.1637426900585, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786584, Length=146, Percent_Identity=36.986301369863, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1787262, Length=245, Percent_Identity=28.1632653061224, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787802, Length=178, Percent_Identity=33.1460674157303, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: NA

Molecular weight: Translated: 49862; Mature: 49730

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYGLKQSVIDTAFAVANIAEYRRSVA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSDECIQVKEFEKNYCADIMQ
HHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHCCCCCEECCCCCEEHHHHHHHHHHHHHH
VVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLERFSLLSKSLAMDSENLMFSL
HHHHHHCCCCCEEEECCCEEEEEEEEEECCCCEEEEECHHHHHHHHHHHHCCCHHHHHHH
FKNGKPVTGDEFNAKNTIFTVSEAMEHFAYLPTGLYVFAYKKDVYLRVCTLIIFFAALVA
HCCCCCCCCCCCCCCCCEEEHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHH
VISGASCLYLVRRVINRGIVEKEAIINNHFERVLDGGLFFSAADVKKLYSMYNSAFLDDL
HHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHH
TKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNINDTFGHLLGDEVLMKVVKILKSQIPV
HHHHCCCCHHHHHHHCCCCCCEEEEEEHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
DKGKVYRFGGDEFAVIYTGGTLEELLSILKEIVHFQVGSINLSTSIGVAHSNECTTVERL
CCCCEEEECCCEEEEEEECCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHH
KMLADERLYKSKKNGRAQISWQ
HHHHHHHHHHHCCCCCEEEEEC
>Mature Secondary Structure 
SRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYGLKQSVIDTAFAVANIAEYRRSVA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSDECIQVKEFEKNYCADIMQ
HHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHCCCCCEECCCCCEEHHHHHHHHHHHHHH
VVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLERFSLLSKSLAMDSENLMFSL
HHHHHHCCCCCEEEECCCEEEEEEEEEECCCCEEEEECHHHHHHHHHHHHCCCHHHHHHH
FKNGKPVTGDEFNAKNTIFTVSEAMEHFAYLPTGLYVFAYKKDVYLRVCTLIIFFAALVA
HCCCCCCCCCCCCCCCCEEEHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHH
VISGASCLYLVRRVINRGIVEKEAIINNHFERVLDGGLFFSAADVKKLYSMYNSAFLDDL
HHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHH
TKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNINDTFGHLLGDEVLMKVVKILKSQIPV
HHHHCCCCHHHHHHHCCCCCCEEEEEEHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
DKGKVYRFGGDEFAVIYTGGTLEELLSILKEIVHFQVGSINLSTSIGVAHSNECTTVERL
CCCCEEEECCCEEEEEEECCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHH
KMLADERLYKSKKNGRAQISWQ
HHHHHHHHHHHCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]