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The map label for this gene is moaA [H]

Identifier: 157160256

GI number: 157160256

Start: 843647

End: 844636

Strand: Direct

Name: moaA [H]

Synonym: EcHS_A0835

Alternate gene names: 157160256

Gene position: 843647-844636 (Clockwise)

Preceding gene: 157160254

Following gene: 157160257

Centisome position: 18.17

GC content: 54.14

Gene sequence:

>990_bases
ATGGCTTCACAACTGACTGATGCATTTGCGCGTAAGTTTTACTACTTGCGCCTGTCGATTACCGATGTGTGTAACTTTCG
TTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCC
GGGTTACGCGCGCCTTCGCCAGTCTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGAGCCGTCTTTACGCCGCGACTTT
ACCGATATCATCGCCGCTGTGCGGGAAAACGACGCTATCCGCCAGATTGCGGTCACCACCAATGGTTACCGTCTGGAACG
CGATGTGGCGAACTGGCGCGATGCGGGACTTACTGGCATTAACGTCAGTGTCGACAGTCTGGACGCCCGCCAGTTTCACG
CTATTACCGGGCAGGATAAATTCAACCAGGTCATGGCAGGGATTGATGCTGCATTTGAGGCCGGTTTTGAGAAGGTCAAA
GTCAATACCGTGCTGATGCGTGATGTTAATCATCACCAGCTCGACACCTTTCTGAACTGGATCCAGCATCGCCCTATCCA
GCTGCGTTTCATCGAACTGATGGAAACGGGCGAGGGCAGTGAGCTCTTCCGTAAACATCACATCTCTGGTCAGGTTCTGC
GTGACGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCG
GATTACGCCGGAGAGATTGGCCTTATCATGCCGTATGAAAAAGACTTCTGCGCCACTTGCAACCGCCTGCGCGTTTCCTC
CATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATGCCCAGCAAC
AGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATT
ACGCAAAACTTATCGTACATTGGCGGCTAA

Upstream 100 bases:

>100_bases
GGTCAACTGATACGGTGCTTTGGCCGTGACAATGCTCGTAAAGATTGCCACCAGGGCGAAGGAAGAAATGACTTCGCCTC
CCGTATCTGGAAAGGTGTAC

Downstream 100 bases:

>100_bases
AACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTATCCCGACCCGTATTGCTATTCTTACGGTTTCTAA
TCGGCGCGGTGAAGAAGACG

Product: molybdenum cofactor biosynthesis protein A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 329; Mature: 328

Protein sequence:

>329_residues
MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNKGFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDF
TDIIAAVRENDAIRQIAVTTNGYRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELLRRGWIHQLRQRSDGPAQVFCHP
DYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLFGEGGVNLRDLLEDDAQQQALEARISAALREKKQTHFLHQNNTGI
TQNLSYIGG

Sequences:

>Translated_329_residues
MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNKGFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDF
TDIIAAVRENDAIRQIAVTTNGYRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELLRRGWIHQLRQRSDGPAQVFCHP
DYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLFGEGGVNLRDLLEDDAQQQALEARISAALREKKQTHFLHQNNTGI
TQNLSYIGG
>Mature_328_residues
ASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNKGFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFT
DIIAAVRENDAIRQIAVTTNGYRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVKV
NTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELLRRGWIHQLRQRSDGPAQVFCHPD
YAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLFGEGGVNLRDLLEDDAQQQALEARISAALREKKQTHFLHQNNTGIT
QNLSYIGG

Specific function: Together with moaC, is involved in the conversion of a guanosine derivative (5'-GTP) into molybdopterin precursor Z [H]

COG id: COG2896

COG function: function code H; Molybdenum cofactor biosynthesis enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. MoaA family [H]

Homologues:

Organism=Homo sapiens, GI116734668, Length=310, Percent_Identity=39.6774193548387, Blast_Score=222, Evalue=3e-58,
Organism=Homo sapiens, GI22027536, Length=310, Percent_Identity=39.6774193548387, Blast_Score=222, Evalue=4e-58,
Organism=Escherichia coli, GI1786999, Length=329, Percent_Identity=99.0881458966565, Blast_Score=679, Evalue=0.0,
Organism=Caenorhabditis elegans, GI32566331, Length=313, Percent_Identity=36.1022364217252, Blast_Score=202, Evalue=3e-52,
Organism=Caenorhabditis elegans, GI32566329, Length=308, Percent_Identity=36.3636363636364, Blast_Score=200, Evalue=9e-52,
Organism=Caenorhabditis elegans, GI17561210, Length=307, Percent_Identity=35.1791530944625, Blast_Score=196, Evalue=1e-50,
Organism=Drosophila melanogaster, GI28574490, Length=315, Percent_Identity=38.0952380952381, Blast_Score=214, Evalue=7e-56,
Organism=Drosophila melanogaster, GI28574488, Length=315, Percent_Identity=38.0952380952381, Blast_Score=214, Evalue=7e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR013483
- InterPro:   IPR000385
- InterPro:   IPR010505
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06463 Mob_synth_C; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 37275; Mature: 37144

Theoretical pI: Translated: 7.85; Mature: 7.85

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNKGFLTVDEIRRVTRAFA
CCCHHHHHHHHHEEEEEEEEEHHCCCEEEEECCCCCCCCCCCCCCCEEHHHHHHHHHHHH
SLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNGYRLERDVANWRDAGLTGI
HCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCEEEEHHHHHHHHCCCEEE
NVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVKVNTVLMRDVNHHQLDTFLNW
EEECCCCCCHHEEEECCCHHHHHHHHHHHHHHHCCCHHEEEEEEEEECCCHHHHHHHHHH
IQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELLRRGWIHQLRQRSDGPAQVFCHP
HHCCCCEEEEEEEHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECC
DYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLFGEGGVNLRDLLEDDAQQQALEARI
CCCCCEEEEECCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
SAALREKKQTHFLHQNNTGITQNLSYIGG
HHHHHHHHHHHHHCCCCCCCCCCCHHCCC
>Mature Secondary Structure 
ASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNKGFLTVDEIRRVTRAFA
CCHHHHHHHHHEEEEEEEEEHHCCCEEEEECCCCCCCCCCCCCCCEEHHHHHHHHHHHH
SLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNGYRLERDVANWRDAGLTGI
HCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCEEEEHHHHHHHHCCCEEE
NVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVKVNTVLMRDVNHHQLDTFLNW
EEECCCCCCHHEEEECCCHHHHHHHHHHHHHHHCCCHHEEEEEEEEECCCHHHHHHHHHH
IQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELLRRGWIHQLRQRSDGPAQVFCHP
HHCCCCEEEEEEEHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECC
DYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLFGEGGVNLRDLLEDDAQQQALEARI
CCCCCEEEEECCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
SAALREKKQTHFLHQNNTGITQNLSYIGG
HHHHHHHHHHHHHCCCCCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA