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The map label for this gene is ybhC

Identifier: 157160247

GI number: 157160247

Start: 832601

End: 833884

Strand: Reverse

Name: ybhC

Synonym: EcHS_A0826

Alternate gene names: 157160247

Gene position: 833884-832601 (Counterclockwise)

Preceding gene: 157160248

Following gene: 157160243

Centisome position: 17.96

GC content: 53.35

Gene sequence:

>1284_bases
GTGAACACATTTTCAGTTTCCCGTCTGGCGCTGGCATTGGCTTTTGGCGTGACGCTGACCGCCTGTAGCTCAACCCCGCC
CGATCAACGTCCTTCTGATCAAACCGCGCCTGGTACCTCTTCTCGCCCGATTCTGTCGGCAAAAGAAGCGCAGAATTTCG
ATGCTCAACACTATTTTGCATCCCTGACACCAGGTGCTGCAGCGTGGAATCCTTCCCCGATTACCCTGCCTGCGCAACCT
GACTTTGTTGTCGGCCCGGCGGGCACTCAAGGTGTAACGCATACCACGATTCAGGCGGCGGTAGATGCGGCAATTATCAA
GCGTACCAACAAGCGCCAGTATATTGCCGTGATGCCTGGTGAGTATCAGGGAACGGTATATGTCCCTGCCGCTCCGGGTG
GAATTACTCTGTACGGTACAGGTGAAAAACCGATTGATGTGAAGATTGGGCTTTCCCTTGATGGTGGCATGAGCCCTGCC
GACTGGCGTCACGACGTCAACCCGCGCGGCAAATATATGCCAGGTAAACCAGCGTGGTATATGTACGATAGCTGCCAGAG
CAAACGCAGCGACAGTATCGGTGTTCTCTGCTCAGCGGTCTTCTGGTCACAAAACAATGGCCTGCAACTGCAAAATCTGA
CCATCGAAAACACGCTGGGCGATAGCGTAGATGCAGGTAACCATCCGGCGGTGGCACTGCGTACTGATGGTGACCAGGTA
CAGATTAACAACGTTAACATTCTCGGTCGTCAGAACACCTTCTTTGTCACCAACAGCGGTGTGCAGAACCGTCTGGAAAC
GAATCGTCAGCCGCGTACGCTGGTGACCAACAGCTACATTGAAGGGGATGTGGATATCGTTTCTGGTCGCGGCGCAGTGG
TGTTCGATAACACCGAATTCCGCGTGGTGAACTCACGTACTCAGCAAGAAGCGTATGTGTTTGCACCGGCTACGCTGTCC
AACATTTACTACGGTTTCCTCGCCGTAAACAGCCGTTTCAATGCTTTCGGTGATGGTGTGGCGCAACTGGGCCGCTCGCT
GGATGTTGATGCCAATACCAACGGTCAGGTGGTGATCCGTGATAGCGCCATCAACGAAGGTTTTAACACGGCTAAACCGT
GGGCCGATGCGGTGATCTCTAATCGTCCGTTTGCGGGTAATACCGGCAGCGTAGATGATAACGACGAAATACAGCGCAAT
CTGAATGACACTAACTACAACCGCATGTGGGAATACAATAACCGCGGCGTGGGTAGTAAAGTGGTTGCAGAGGCGAAGAA
GTAA

Upstream 100 bases:

>100_bases
AACAACTTTTTGTCTTTTTACCTTCCCGTTTCGCTCAAGTTAGTATAAAAAAGCAGGCTTCAACGGATTCATTTTTCTAT
TTCATAGACCGGAGCAACCT

Downstream 100 bases:

>100_bases
GAGCAATTAACTATTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGTTCGATGCGATTTGTAGGTCGGATAAGA
TGCGCAAGCATCGCATCCGA

Product: putative pectinesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPILSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQP
DFVVGPAGTQGVTHTTIQAAVDAAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENTLGDSVDAGNHPAVALRTDGDQV
QINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVTNSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLS
NIYYGFLAVNSRFNAFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVDDNDEIQRN
LNDTNYNRMWEYNNRGVGSKVVAEAKK

Sequences:

>Translated_427_residues
MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPILSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQP
DFVVGPAGTQGVTHTTIQAAVDAAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENTLGDSVDAGNHPAVALRTDGDQV
QINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVTNSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLS
NIYYGFLAVNSRFNAFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVDDNDEIQRN
LNDTNYNRMWEYNNRGVGSKVVAEAKK
>Mature_427_residues
MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPILSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQP
DFVVGPAGTQGVTHTTIQAAVDAAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENTLGDSVDAGNHPAVALRTDGDQV
QINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVTNSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLS
NIYYGFLAVNSRFNAFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVDDNDEIQRN
LNDTNYNRMWEYNNRGVGSKVVAEAKK

Specific function: Putative thioesterase. Does not bind pectin, and has no pectinesterase activity

COG id: COG4677

COG function: function code G; Pectin methylesterase

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pectinesterase family

Homologues:

Organism=Escherichia coli, GI1786989, Length=427, Percent_Identity=100, Blast_Score=877, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YBHC_ECOLI (P46130)

Other databases:

- EMBL:   U00096
- EMBL:   AP009048
- EMBL:   U39938
- EMBL:   J01638
- PIR:   D64813
- RefSeq:   AP_001403.1
- RefSeq:   NP_415293.1
- PDB:   3GRH
- PDBsum:   3GRH
- ProteinModelPortal:   P46130
- SMR:   P46130
- DIP:   DIP-11407N
- MINT:   MINT-1240146
- STRING:   P46130
- EnsemblBacteria:   EBESCT00000002978
- EnsemblBacteria:   EBESCT00000016863
- GeneID:   945381
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW0755
- KEGG:   eco:b0772
- EchoBASE:   EB2713
- EcoGene:   EG12875
- eggNOG:   COG4677
- GeneTree:   EBGT00050000010934
- HOGENOM:   HBG416454
- OMA:   GVMCSAV
- ProtClustDB:   PRK10531
- BioCyc:   EcoCyc:EG12875-MONOMER
- BRENDA:   3.1.2.2
- Genevestigator:   P46130
- InterPro:   IPR012334
- InterPro:   IPR011050
- InterPro:   IPR018040
- InterPro:   IPR000070
- Gene3D:   G3DSA:2.160.20.10

Pfam domain/function: PF01095 Pectinesterase; SSF51126 Pectin_lyas_like

EC number: NA

Molecular weight: Translated: 46083; Mature: 46083

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: PS00800 PECTINESTERASE_1; PS00503 PECTINESTERASE_2

Important sites: ACT_SITE 285-285 BINDING 345-345

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPILSAKEAQNFDAQHYFA
CCCCHHHHHHHHHHHHCEEEECCCCCCCCCCCCCCCCCCCCCCEEEHHHCCCCCHHHEEE
SLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVDAAIIKRTNKRQYIAVMPG
ECCCCCCCCCCCCEEECCCCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECC
EYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPADWRHDVNPRGKYMPGKPAWY
CCCCEEEEECCCCCEEEEECCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEE
MYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENTLGDSVDAGNHPAVALRTDGDQV
EECCHHCCCCCCCEEEEEEEEEECCCCEEEEEEEEECCCCCCCCCCCCCEEEEEECCCEE
QINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVTNSYIEGDVDIVSGRGAVVFDNTEF
EEEEEEEEECCCEEEEECCCHHHHHHCCCCCCEEEECCEECCCEEEEECCCEEEECCCEE
RVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFNAFGDGVAQLGRSLDVDANTNGQVVIR
EEECCCCCCCEEEECCHHHHHEEEEEEEECCCCCCCCHHHHHHCCEEECCCCCCCEEEEE
DSAINEGFNTAKPWADAVISNRPFAGNTGSVDDNDEIQRNLNDTNYNRMWEYNNRGVGSK
ECCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCCCEEEEECCCCCCHH
VVAEAKK
HHHCCCC
>Mature Secondary Structure
MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPILSAKEAQNFDAQHYFA
CCCCHHHHHHHHHHHHCEEEECCCCCCCCCCCCCCCCCCCCCCEEEHHHCCCCCHHHEEE
SLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVDAAIIKRTNKRQYIAVMPG
ECCCCCCCCCCCCEEECCCCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECC
EYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPADWRHDVNPRGKYMPGKPAWY
CCCCEEEEECCCCCEEEEECCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEE
MYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENTLGDSVDAGNHPAVALRTDGDQV
EECCHHCCCCCCCEEEEEEEEEECCCCEEEEEEEEECCCCCCCCCCCCCEEEEEECCCEE
QINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVTNSYIEGDVDIVSGRGAVVFDNTEF
EEEEEEEEECCCEEEEECCCHHHHHHCCCCCCEEEECCEECCCEEEEECCCEEEECCCEE
RVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFNAFGDGVAQLGRSLDVDANTNGQVVIR
EEECCCCCCCEEEECCHHHHHEEEEEEEECCCCCCCCHHHHHHCCEEECCCCCCCEEEEE
DSAINEGFNTAKPWADAVISNRPFAGNTGSVDDNDEIQRNLNDTNYNRMWEYNNRGVGSK
ECCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCCCEEEEECCCCCCHH
VVAEAKK
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9278503; 8905232; 331474; 7567469; 10806384