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The map label for this gene is nagC

Identifier: 157160154

GI number: 157160154

Start: 730663

End: 731883

Strand: Reverse

Name: nagC

Synonym: EcHS_A0720

Alternate gene names: 157160154

Gene position: 731883-730663 (Counterclockwise)

Preceding gene: 157160155

Following gene: 157160153

Centisome position: 15.76

GC content: 53.15

Gene sequence:

>1221_bases
ATGACACCAGGCGGACAAGCTCAGATAGGTAATGTTGATCTCGTAAAACAGCTTAACAGCGCGGCGGTTTATCGCCTGAT
TGACCAGTACGGGCCAATCTCGCGGATTCAGATTGCCGAGCAAAGCCAGCTTGCCCCCGCCAGCGTAACCAAAATTACGC
GTCAGCTTATCGAACGCGGGCTGATCAAAGAAGTTGATCAGCAGGCCTCCACCGGGGGCCGCCGCGCTATCTCTATCGTC
ACCGAAACCCGCAATTTCCACGCAATCGGTGTACGGCTTGGTCGTCACGACGCCACCATTACTCTGTTTGATCTCAGCAG
CAAAGTGCTGGCAGAAGAACATTACCCGCTGCCGGAACGTACCCAGCAGACGCTGGAACATGCCCTGCTGAATGCCATTG
CTCAGTTTATTGATAGCTACCAACGCAAGCTGCGCGAGCTGATCGCCATTTCCGTGATCCTGCCTGGACTTGTTGACCCG
GACAGCGGCAAAATTCATTACATGCCGCATATTCAGGTGGAAAACTGGGGGCTGGTAGAAGCACTGGAAGAGCGTTTTAA
AGTGACCTGTTTCGTTGGTCACGATATCCGTAGTCTGGCGCTGGCAGAGCACTACTTCGGTGCAAGTCAGGATTGCGAAG
ACTCCATTTTAGTGCGTGTCCATCGCGGAACCGGGGCCGGAATCATCTCTAACGGGCGCATTTTTATTGGCCGCAACGGC
AACGTCGGTGAAATTGGCCATATTCAGGTCGAACCGCTGGGTGAACGCTGCCACTGCGGCAACTTTGGCTGCCTGGAAAC
TATCGCCGCCAACGCTGCCATTGAACAACGGGTGTTGAATCTGTTAAAGCAGGGCTACCAGAGCCGCGTGCCGCTGGACG
ACTGTACCATCAAAACTATCTGCAAAGCTGCGAACAAAGGCGATAGCCTGGCGTCGGAAGTGATTGAGTATGTTGGTCGT
CATCTGGGCAAAACCATCGCCATTGCCATCAACCTGTTTAACCCGCAAAAAATTGTTATTGCCGGAGAAATCACCGAAGC
CGATAAAGTGCTGCTCCCTGCTATTGAAAGCTGCATTAATACCCAGGCGCTGAAGGCGTTTCGTACTAATCTGCCGGTGG
TACGTTCTGAGCTGGATCACCGCTCGGCAATCGGCGCTTTTGCGCTGGTAAAACGCGCCATGCTCAACGGTATTTTGCTC
CAGCATTTGCTGGAAAATTAA

Upstream 100 bases:

>100_bases
CCGCAGGTAAAGTAGCCAACCTGACCGCATTCACACCTGATTTTAAAATCACCAAGACCATCGTTAACGGTAACGAGGTC
GTAACTCAATAAGAGAAAGT

Downstream 100 bases:

>100_bases
TGTGCTTTTATAGTGGCGCTTATTGTTGTCAATATTCTGGGTAGTCCATGACCATTAAAAATGTAATTTGCGATATCGAC
GGCGTGCTGATGCACGATAA

Product: N-acetylglucosamine repressor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 406; Mature: 405

Protein sequence:

>406_residues
MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQLAPASVTKITRQLIERGLIKEVDQQASTGGRRAISIV
TETRNFHAIGVRLGRHDATITLFDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILVRVHRGTGAGIISNGRIFIGRNG
NVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQRVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGR
HLGKTIAIAINLFNPQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKRAMLNGILL
QHLLEN

Sequences:

>Translated_406_residues
MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQLAPASVTKITRQLIERGLIKEVDQQASTGGRRAISIV
TETRNFHAIGVRLGRHDATITLFDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILVRVHRGTGAGIISNGRIFIGRNG
NVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQRVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGR
HLGKTIAIAINLFNPQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKRAMLNGILL
QHLLEN
>Mature_405_residues
TPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQLAPASVTKITRQLIERGLIKEVDQQASTGGRRAISIVT
ETRNFHAIGVRLGRHDATITLFDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDPD
SGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILVRVHRGTGAGIISNGRIFIGRNGN
VGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQRVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRH
LGKTIAIAINLFNPQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKRAMLNGILLQ
HLLEN

Specific function: Acts as a repressor of the nagEBACD operon and acts both as an activator and a repressor for the transcription of the glmSU operon

COG id: COG1940

COG function: function code KG; Transcriptional regulator/sugar kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family

Homologues:

Organism=Escherichia coli, GI1786891, Length=406, Percent_Identity=100, Blast_Score=828, Evalue=0.0,
Organism=Escherichia coli, GI1787878, Length=400, Percent_Identity=40.5, Blast_Score=309, Evalue=2e-85,
Organism=Escherichia coli, GI87082127, Length=345, Percent_Identity=24.6376811594203, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI87081733, Length=272, Percent_Identity=22.7941176470588, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1790522, Length=293, Percent_Identity=24.5733788395904, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NAGC_ECO57 (P0AF22)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   B85567
- PIR:   B90717
- RefSeq:   NP_286390.1
- RefSeq:   NP_308733.1
- ProteinModelPortal:   P0AF22
- SMR:   P0AF22
- EnsemblBacteria:   EBESCT00000025296
- EnsemblBacteria:   EBESCT00000058958
- GeneID:   917075
- GeneID:   957745
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z0823
- KEGG:   ecs:ECs0706
- GeneTree:   EBGT00050000009665
- HOGENOM:   HBG297939
- OMA:   DALACEV
- ProtClustDB:   CLSK870201
- BioCyc:   ECOL83334:ECS0706-MONOMER
- GO:   GO:0005622
- InterPro:   IPR000835
- InterPro:   IPR000600
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10

Pfam domain/function: PF01047 MarR; PF00480 ROK

EC number: NA

Molecular weight: Translated: 44542; Mature: 44411

Theoretical pI: Translated: 7.38; Mature: 7.38

Prosite motif: PS01125 ROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQLAPASVTKITRQLIERG
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECHHCCCCHHHHHHHHHHHHHHH
LIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITLFDLSSKVLAEEHYPLPER
HHHHHHHHHCCCCCEEEEEEEECCCEEEEEEEECCCCCEEEEEECCHHHHHHCCCCCCHH
TQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDPDSGKIHYMPHIQVENWGLVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEECCCCHHH
ALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILVRVHRGTGAGIISNGRIFIGRNG
HHHHHHEEEEEECCCHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCEEECCEEEEECCC
NVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQRVLNLLKQGYQSRVPLDDCTIKTI
CCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
CKAANKGDSLASEVIEYVGRHLGKTIAIAINLFNPQKIVIAGEITEADKVLLPAIESCIN
HHHCCCCHHHHHHHHHHHHHHHCCEEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHH
TQALKAFRTNLPVVRSELDHRSAIGAFALVKRAMLNGILLQHLLEN
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQLAPASVTKITRQLIERG
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECHHCCCCHHHHHHHHHHHHHHH
LIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITLFDLSSKVLAEEHYPLPER
HHHHHHHHHCCCCCEEEEEEEECCCEEEEEEEECCCCCEEEEEECCHHHHHHCCCCCCHH
TQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDPDSGKIHYMPHIQVENWGLVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEECCCCHHH
ALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILVRVHRGTGAGIISNGRIFIGRNG
HHHHHHEEEEEECCCHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCEEECCEEEEECCC
NVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQRVLNLLKQGYQSRVPLDDCTIKTI
CCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
CKAANKGDSLASEVIEYVGRHLGKTIAIAINLFNPQKIVIAGEITEADKVLLPAIESCIN
HHHCCCCHHHHHHHHHHHHHHHCCEEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHH
TQALKAFRTNLPVVRSELDHRSAIGAFALVKRAMLNGILLQHLLEN
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796