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The map label for this gene is mrdA

Identifier: 157160130

GI number: 157160130

Start: 702401

End: 704302

Strand: Reverse

Name: mrdA

Synonym: EcHS_A0687

Alternate gene names: 157160130

Gene position: 704302-702401 (Counterclockwise)

Preceding gene: 157160131

Following gene: 157160129

Centisome position: 15.17

GC content: 52.63

Gene sequence:

>1902_bases
ATGAAACTACAGAACTCTTTTCGCGACTATACGGCAGAGTCCGCGCTGTTTGTGCGCCGGGCGCTGGTCGCCTTTTTGGG
GATTTTGCTGCTGACCGGCGTGCTTATCGCCAACCTGTATAATCTGCAAATTGTTCGCTTTACCGACTACCAGACTCGCT
CTAATGAAAACCGCATTAAGCTGGTGCCTATCGCGCCCAGCCGCGGCATTATCTACGACCGTAATGGTATCCCTCTGGCC
CTCAACCGCACTATCTACCAGATAGAAATGATGCCAGAGAAAGTCGATAACGTGCAGCAAACGCTGGACGCTTTGCGCAG
CGTGGTTGATCTGACCGATGACGATATTGCTGCATTCCGAAAAGAGCGCGCACGTTCACACCGTTTCACCTCTATTCCGG
TGAAAACTAACCTGACCGAAGTACAAGTAGCTCGCTTTGCCGTCAATCAGTACCGTTTTCCGGGTGTCGAAGTTAAAGGC
TATAAACGTCGTTACTATCCTTACGGTTCGGCGTTGACCCACGTCATCGGCTATGTGTCGAAAATCAACGATAAAGACGT
CGAACGCCTGAATAATGACGGCAAACTGGCCAACTATGCGGCAACGCATGATATCGGTAAGCTGGGCATTGAGCGTTACT
ATGAAGACGTGCTGCACGGTCAGACCGGTTATGAAGAGGTTGAAGTTAACAACCGTGGGCGTGTTATCCGCCAGTTAAAA
GAAGTACCACCGCAAGCCGGACACGATATTTACCTGACGCTGGATCTCAAACTCCAGCAATATATTGAAACGCTGCTGGC
GGGTAGCCGCGCAGCTGTGGTTGTCACCGATCCGCGTACAGGTGGGGTACTGGCGCTGGTTTCCACGCCTAGTTATGACC
CAAACCTGTTTGTTGACGGTATCTCCAGCAAAGATTATTCCGCCTTGTTGAACGACCCGAATACACCGCTGGTGAACCGC
GCCACACAGGGGGTTTATCCTCCCGCGTCTACAGTTAAACCCTATGTGGCGGTTTCGGCATTGAGCGCCGGGGTGATCAC
GCGCAATACGACGCTGTTTGACCCAGGCTGGTGGCAACTGCCAGGTTCGGAAAAACGTTATCGTGACTGGAAAAAATGGG
GCCACGGGCGTCTGAATGTCACAAGATCGCTGGAAGAATCTGCGGATACCTTCTTCTATCAGGTGGCCTACGATATGGGG
ATCGATCGCCTCTCCGAATGGATGGGTAAATTCGGTTATGGTCATTACACCGGTATCGACCTGGCGGAAGAACGTTCCGG
CAACATGCCTACCCGCGAATGGAAACAGAAACGCTTTAAAAAACCGTGGTATCAGGGTGACACCATTCCGGTTGGTATCG
GTCAGGGTTACTGGACAGCGACCCCAATCCAGATGAGTAAGGCACTGATGATCCTGATTAATGACGGTATCGTGAAGGTT
CCTCATTTGCTGATGAGCACCGCCGAAGACGGCAAACAGGTGCCATGGGTACAGCCGCATGAACCGCCCGTCGGCGATAT
TCATTCCGGTTACTGGGAGCTGGCGAAAGACGGTATGTACGGTGTTGCTAACCGCCCTAACGGTACGGCGCATAAATACT
TTGCCAGCGCACCGTACAAAATTGCGGCGAAATCCGGTACCGCTCAGGTCTTCGGTCTGAAAGCGAACGAAACCTATAAT
GCGCACAAAATTGCCGAGCGTTTACGTGACCACAAACTGATGACCGCCTTTGCGCCATACAACAATCCGCAAGTGGCTGT
CGCCATGATTCTGGAGAACGGTGGTGCGGGTCCGGCGGTTGGTACACTGATGCGCCAGATCCTCGACCACATTATGCTGG
GTGATAACAACACCGATCTGCCTGCGGAAAATCCAGCGGTTGCCGCAGCGGAGGACCATTAA

Upstream 100 bases:

>100_bases
CGTGCTGGTCGCAGAGAGTCTGTACCGGGCGTGGAGCATCACCACCAACCATCCTTATCACCGTGAGTGATAAGGGAGCT
TTGAGTAGAAAACGCAGCGG

Downstream 100 bases:

>100_bases
TCATGACGGATAATCCGAATAAAAAAACATTCTGGGATAAAGTCCATCTCGATCCCACAATGCTGCTGATCTTACTGGCA
TTGCTGGTTTACAGCGCCCT

Product: penicillin-binding protein 2

Products: NA

Alternate protein names: PBP-2

Number of amino acids: Translated: 633; Mature: 633

Protein sequence:

>633_residues
MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQIVRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLA
LNRTIYQIEMMPEKVDNVQQTLDALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVLHGQTGYEEVEVNNRGRVIRQLK
EVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVTDPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNR
ATQGVYPPASTVKPYVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFFYQVAYDMG
IDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIPVGIGQGYWTATPIQMSKALMILINDGIVKV
PHLLMSTAEDGKQVPWVQPHEPPVGDIHSGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYN
AHKIAERLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAENPAVAAAEDH

Sequences:

>Translated_633_residues
MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQIVRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLA
LNRTIYQIEMMPEKVDNVQQTLDALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVLHGQTGYEEVEVNNRGRVIRQLK
EVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVTDPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNR
ATQGVYPPASTVKPYVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFFYQVAYDMG
IDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIPVGIGQGYWTATPIQMSKALMILINDGIVKV
PHLLMSTAEDGKQVPWVQPHEPPVGDIHSGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYN
AHKIAERLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAENPAVAAAEDH
>Mature_633_residues
MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQIVRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLA
LNRTIYQIEMMPEKVDNVQQTLDALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVLHGQTGYEEVEVNNRGRVIRQLK
EVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVTDPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNR
ATQGVYPPASTVKPYVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFFYQVAYDMG
IDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIPVGIGQGYWTATPIQMSKALMILINDGIVKV
PHLLMSTAEDGKQVPWVQPHEPPVGDIHSGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYN
AHKIAERLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAENPAVAAAEDH

Specific function: Cell wall formation; PBP-2 is responsible for the determination of the rod shape of the cell. Its synthesize cross- linked peptidoglycan from the lipid intermediates

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell inner membrane

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family

Homologues:

Organism=Escherichia coli, GI1786854, Length=633, Percent_Identity=100, Blast_Score=1306, Evalue=0.0,
Organism=Escherichia coli, GI1786272, Length=636, Percent_Identity=25, Blast_Score=139, Evalue=8e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PBP2_ECO57 (P0AD67)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   A90713
- PIR:   E85563
- RefSeq:   NP_286361.1
- RefSeq:   NP_308700.1
- ProteinModelPortal:   P0AD67
- SMR:   P0AD67
- EnsemblBacteria:   EBESCT00000026234
- EnsemblBacteria:   EBESCT00000055123
- GeneID:   917034
- GeneID:   957678
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z0781
- KEGG:   ecs:ECs0673
- GeneTree:   EBGT00050000010128
- HOGENOM:   HBG712588
- OMA:   WKKKTFK
- ProtClustDB:   PRK10795
- BioCyc:   ECOL83334:ECS0673-MONOMER
- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460
- InterPro:   IPR017790
- TIGRFAMs:   TIGR03423

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase; SSF56519 PBP_dimer; SSF56601 PBP_transp_fold

EC number: NA

Molecular weight: Translated: 70857; Mature: 70857

Theoretical pI: Translated: 9.16; Mature: 9.16

Prosite motif: NA

Important sites: ACT_SITE 330-330

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQIVRFTDYQTRSNENRIK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCCCCCEEE
LVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTLDALRSVVDLTDDDIAAFR
EEEECCCCCEEECCCCCCEEECCEEEEEEECHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
KERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKGYKRRYYPYGSALTHVIGYVS
HHHHHCCCEEECCCCCCCHHHHHHHHHHHHHCCCCCEEECCEEEECCCHHHHHHHHHHHH
KINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVLHGQTGYEEVEVNNRGRVIRQLK
HCCHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHH
EVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVTDPRTGGVLALVSTPSYDPNLFVDG
HCCCCCCCEEEEEEEHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEECCCCCCCEEEEC
ISSKDYSALLNDPNTPLVNRATQGVYPPASTVKPYVAVSALSAGVITRNTTLFDPGWWQL
CCCCCHHHHHCCCCCCHHHHCCCCCCCCHHCCCHHHHHHHHHCCEEECCCEEECCCCEEC
PGSEKRYRDWKKWGHGRLNVTRSLEESADTFFYQVAYDMGIDRLSEWMGKFGYGHYTGID
CCCCHHHHHHHHHCCCEEEEEHHHHHHHHHEEEEHHHHHCHHHHHHHHHHCCCCCCCCCC
LAEERSGNMPTREWKQKRFKKPWYQGDTIPVGIGQGYWTATPIQMSKALMILINDGIVKV
CHHHCCCCCCHHHHHHHHHCCCCCCCCEEEEECCCCCEECCHHHHCCEEEEEEECCCHHH
PHLLMSTAEDGKQVPWVQPHEPPVGDIHSGYWELAKDGMYGVANRPNGTAHKYFASAPYK
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCEECCCCCCCCHHHEECCCCEE
IAAKSGTAQVFGLKANETYNAHKIAERLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAV
EEECCCCEEEEEEECCCCCCHHHHHHHHHHCCEEEEECCCCCCCEEEEEEEECCCCCHHH
GTLMRQILDHIMLGDNNTDLPAENPAVAAAEDH
HHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQIVRFTDYQTRSNENRIK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCCCCCEEE
LVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTLDALRSVVDLTDDDIAAFR
EEEECCCCCEEECCCCCCEEECCEEEEEEECHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
KERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKGYKRRYYPYGSALTHVIGYVS
HHHHHCCCEEECCCCCCCHHHHHHHHHHHHHCCCCCEEECCEEEECCCHHHHHHHHHHHH
KINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVLHGQTGYEEVEVNNRGRVIRQLK
HCCHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHH
EVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVTDPRTGGVLALVSTPSYDPNLFVDG
HCCCCCCCEEEEEEEHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEECCCCCCCEEEEC
ISSKDYSALLNDPNTPLVNRATQGVYPPASTVKPYVAVSALSAGVITRNTTLFDPGWWQL
CCCCCHHHHHCCCCCCHHHHCCCCCCCCHHCCCHHHHHHHHHCCEEECCCEEECCCCEEC
PGSEKRYRDWKKWGHGRLNVTRSLEESADTFFYQVAYDMGIDRLSEWMGKFGYGHYTGID
CCCCHHHHHHHHHCCCEEEEEHHHHHHHHHEEEEHHHHHCHHHHHHHHHHCCCCCCCCCC
LAEERSGNMPTREWKQKRFKKPWYQGDTIPVGIGQGYWTATPIQMSKALMILINDGIVKV
CHHHCCCCCCHHHHHHHHHCCCCCCCCEEEEECCCCCEECCHHHHCCEEEEEEECCCHHH
PHLLMSTAEDGKQVPWVQPHEPPVGDIHSGYWELAKDGMYGVANRPNGTAHKYFASAPYK
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCEECCCCCCCCHHHEECCCCEE
IAAKSGTAQVFGLKANETYNAHKIAERLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAV
EEECCCCEEEEEEECCCCCCHHHHHHHHHHCCEEEEECCCCCCCEEEEEEEECCCCCHHH
GTLMRQILDHIMLGDNNTDLPAENPAVAAAEDH
HHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796