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The map label for this gene is lipA [H]

Identifier: 157160123

GI number: 157160123

Start: 695334

End: 696299

Strand: Reverse

Name: lipA [H]

Synonym: EcHS_A0679

Alternate gene names: 157160123

Gene position: 696299-695334 (Counterclockwise)

Preceding gene: 157160124

Following gene: 157160120

Centisome position: 15.0

GC content: 53.31

Gene sequence:

>966_bases
ATGAGTAAACCCATTGTGATGGAACGCGGTGTTAAATACCGCGATGCCGATAAGATGGCCCTTATCCCGGTTAAAAACGT
GGCAACAGAGCGCGAAGCCCTGCTGCGCAAGCCGGAATGGATGAAAATCAAGCTTCCAGCGGACTCTACACGTATCCAGG
GCATCAAAGCCGCAATGCGCAAAAATGGCCTGCATTCTGTCTGCGAGGAAGCCTCCTGCCCTAACCTGGCGGAATGCTTC
AACCACGGCACAGCAACGTTTATGATCCTCGGCGCTATTTGTACCCGCCGTTGTCCGTTCTGTGATGTTGCCCACGGTCG
CCCGGTAGCTCCTGATGCCAATGAACCAGTGAAACTGGCGCAGACCATTGCCGATATGGCGCTGCGTTATGTGGTTATCA
CCTCCGTTGACCGTGATGACCTGCGCGATGGCGGTGCCCAGCACTTTGCGGATTGCATTACTGCCATTCGGGAAAAAAGC
CCGCAAATCAAAATTGAAACTCTGGTGCCGGATTTCCGTGGTCGTATGGATCGTGCTCTGGATATTCTGACTGCAACGCC
ACCAGATGTGTTCAACCATAACCTGGAAAACGTACCGCGTATTTACCGTCAGGTACGGCCTGGTGCAGATTACAACTGGT
CGCTGAAGCTGCTGGAACGCTTTAAAGAAGCGCATCCGGAAATCCCGACCAAGTCTGGTCTGATGGTGGGACTGGGTGAA
ACCAATGAAGAAATTATTGAGGTAATGCGCGACCTGCGCCGTCATGGTGTGACGATGTTAACGCTGGGGCAATATTTGCA
GCCAAGCCGCCATCACCTGCCGGTTCAACGTTACGTTAGCCCGGATGAGTTCGACGAAATGAAAGCCGAAGCGCTGGCGA
TGGGCTTTACCCATGCTGCATGCGGTCCGTTTGTCCGCTCTTCTTACCACGCCGATTTGCAGGCGAAAGGGATGGAAGTT
AAGTAA

Upstream 100 bases:

>100_bases
GATATACTGCCAGTCGTTAATTCAAAAATAGTTGATAATTACAACAATCTATTGAATTGAAACGCTTTCCTTCGTAATTC
GCAACTGGAACACGCACGCT

Downstream 100 bases:

>100_bases
TCCTGTAATAATTACTTACATCTGTCTGATAAAAAACCCGCTTTTTGAAGCGGGTTTTTTGTATCGAACAAGATATTGAG
GGAGCGTCCTGCTCGCCACG

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA [H]

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIKLPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECF
NHGTATFMILGAICTRRCPFCDVAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLLERFKEAHPEIPTKSGLMVGLGE
TNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQRYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEV
K

Sequences:

>Translated_321_residues
MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIKLPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECF
NHGTATFMILGAICTRRCPFCDVAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLLERFKEAHPEIPTKSGLMVGLGE
TNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQRYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEV
K
>Mature_320_residues
SKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIKLPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFN
HGTATFMILGAICTRRCPFCDVAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKSP
QIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLLERFKEAHPEIPTKSGLMVGLGET
NEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQRYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=293, Percent_Identity=42.320819112628, Blast_Score=253, Evalue=2e-67,
Organism=Homo sapiens, GI37577164, Length=258, Percent_Identity=42.6356589147287, Blast_Score=220, Evalue=1e-57,
Organism=Escherichia coli, GI1786846, Length=321, Percent_Identity=100, Blast_Score=669, Evalue=0.0,
Organism=Caenorhabditis elegans, GI32564533, Length=287, Percent_Identity=40.7665505226481, Blast_Score=219, Evalue=2e-57,
Organism=Saccharomyces cerevisiae, GI6324770, Length=303, Percent_Identity=43.5643564356436, Blast_Score=253, Evalue=3e-68,
Organism=Drosophila melanogaster, GI221513272, Length=287, Percent_Identity=44.5993031358885, Blast_Score=244, Evalue=4e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 36072; Mature: 35941

Theoretical pI: Translated: 8.03; Mature: 8.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIKLPADSTRIQGIKAAMR
CCCCCCHHCCCCCCCCCCEEEEEHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHH
KNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCDVAHGRPVAPDANEPVKLA
HCCHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHH
QTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKSPQIKIETLVPDFRGRMDRAL
HHHHHHHHHHHHEECCCHHHHHCCCHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHH
DILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLLERFKEAHPEIPTKSGLMVGLGE
HHHHCCCHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEECCC
TNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQRYVSPDEFDEMKAEALAMGFTHAA
CHHHHHHHHHHHHHCCCEEEEHHHHHCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHH
CGPFVRSSYHADLQAKGMEVK
HHHHHHCCCCCCHHCCCCCCC
>Mature Secondary Structure 
SKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIKLPADSTRIQGIKAAMR
CCCCCHHCCCCCCCCCCEEEEEHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHH
KNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCDVAHGRPVAPDANEPVKLA
HCCHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHH
QTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKSPQIKIETLVPDFRGRMDRAL
HHHHHHHHHHHHEECCCHHHHHCCCHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHH
DILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLLERFKEAHPEIPTKSGLMVGLGE
HHHHCCCHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEECCC
TNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQRYVSPDEFDEMKAEALAMGFTHAA
CHHHHHHHHHHHHHCCCEEEEHHHHHCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHH
CGPFVRSSYHADLQAKGMEVK
HHHHHHCCCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA