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The map label for this gene is allS [H]

Identifier: 157160027

GI number: 157160027

Start: 590872

End: 591798

Strand: Reverse

Name: allS [H]

Synonym: EcHS_A0578

Alternate gene names: 157160027

Gene position: 591798-590872 (Counterclockwise)

Preceding gene: 157160038

Following gene: 157160026

Centisome position: 12.74

GC content: 52.54

Gene sequence:

>927_bases
ATGTTCGATCCAGAAACCTTGCGGACTTTCATTGCGGTTGCTGAAACAGGAGGTTTTTCAAAAGCGGCAGAACGATTATG
TAAAACCACGGCGACGATCAGTTATCGCATTAAACTTCTGGAAGAGAATACCGGAGTAGCGCTGTTTTTCCGTACGACTC
GCAGCGTGACGTTGACAGCGGCTGGCGAGCATCTACTTTCCCAGGCCAGAGACTGGCTGAGCTGGCTGGAAAGTATGCCA
AGCGAGCTGCAACAGGTGAATGATGGCGTGGAACGCCAGGTGAATATTGTCATCAACAACCTGCTCTACAACCCCCAGGC
CGTCGCCCAGTTGCTGGCGTGGCTGAATGAGCGTTACCCCTTTACCCAGTTTCACATCTCCCGACAAATCTATATGGGCG
TCTGGGACTCGCTATTGTACGAAGGTTTTTCGCTGGCTATCGGCGTCACGGGAACTGAGGCGCTGGCAAATACCTTTAGT
CTTGATCCCTTAGGATCGGTGCAATGGCGCTTTGTCATGGCGGCGGATCATCCGCTGGCGAACGTTGAAGAGCCGCTAAC
AGAAGCGCAGTTGCGGCGCTTTCCGGCGGTCAATATTGAAGACAGCGCCCGCACCTTAACCAAACGCGTCGCCTGGCGAT
TGCCAGGGCAAAAAGAGATTATTGTTCCCGATATGGAAACGAAAATCGCCGCCCATCTGGCGGGCGTTGGCATTGGTTTT
TTGCCAAAATCGCTTTGCCAGTCAATGATCGATAATCAACAACTGGTCAGCCGGGTAATCCCAACGATGCGCCCTCCTTC
GCCATTGAGTCTGGCATGGCGCAAATTTGGCAGCGGCAAAGCGGTAGAAGATATTGTGACCTTGTTTACCCAGCGCAGGC
CGGAAATCAGCGGATTTTTAGAAATTTTCGGCAACCCACGCAGTTAA

Upstream 100 bases:

>100_bases
TTTAGTCCACAAATTGAACAGGGTTATCATGTAAAAATACTGATAAATCAGAGGATAAGAAAATCACGCAACTTGCGGAT
TTAAAAGGGGATACCTGAAG

Downstream 100 bases:

>100_bases
AAATCAAGTTTCCTTGCGCGTTGGCGCGTAAAATAGCGCGCAAGCTGATTTGAGGTTCACTTTTAACTATGCAAGAGAGA
CACACGGAACAGGACTATCG

Product: DNA-binding transcriptional activator AllS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MFDPETLRTFIAVAETGGFSKAAERLCKTTATISYRIKLLEENTGVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMP
SELQQVNDGVERQVNIVINNLLYNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQKEIIVPDMETKIAAHLAGVGIGF
LPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKFGSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS

Sequences:

>Translated_308_residues
MFDPETLRTFIAVAETGGFSKAAERLCKTTATISYRIKLLEENTGVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMP
SELQQVNDGVERQVNIVINNLLYNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQKEIIVPDMETKIAAHLAGVGIGF
LPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKFGSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS
>Mature_308_residues
MFDPETLRTFIAVAETGGFSKAAERLCKTTATISYRIKLLEENTGVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMP
SELQQVNDGVERQVNIVINNLLYNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQKEIIVPDMETKIAAHLAGVGIGF
LPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKFGSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS

Specific function: Positive regulator essential for the expression of AllD operon. Binds to the AllD promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786713, Length=308, Percent_Identity=99.6753246753247, Blast_Score=630, Evalue=0.0,
Organism=Escherichia coli, GI1786508, Length=283, Percent_Identity=38.1625441696113, Blast_Score=212, Evalue=2e-56,
Organism=Escherichia coli, GI1787949, Length=275, Percent_Identity=30.5454545454545, Blast_Score=152, Evalue=2e-38,
Organism=Escherichia coli, GI87082024, Length=277, Percent_Identity=24.5487364620939, Blast_Score=111, Evalue=5e-26,
Organism=Escherichia coli, GI1789492, Length=281, Percent_Identity=26.3345195729537, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1787879, Length=258, Percent_Identity=27.906976744186, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI145693105, Length=249, Percent_Identity=29.718875502008, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1788748, Length=123, Percent_Identity=30.8943089430894, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34482; Mature: 34482

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDPETLRTFIAVAETGGFSKAAERLCKTTATISYRIKLLEENTGVALFFRTTRSVTLTA
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHEEEEEEEEECCCCEEEEEECCCCEEEEH
AGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLLYNPQAVAQLLAWLNERYP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCC
FTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFSLDPLGSVQWRFVMAADHPLA
CHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHCCCCCCCCCEEEEEEECCCCCC
NVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQKEIIVPDMETKIAAHLAGVGIGF
CHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHCCHHH
LPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKFGSGKAVEDIVTLFTQRRPEISGFL
HHHHHHHHHHCCHHHHHHHHCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHH
EIFGNPRS
HHHCCCCC
>Mature Secondary Structure
MFDPETLRTFIAVAETGGFSKAAERLCKTTATISYRIKLLEENTGVALFFRTTRSVTLTA
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHEEEEEEEEECCCCEEEEEECCCCEEEEH
AGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLLYNPQAVAQLLAWLNERYP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCC
FTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFSLDPLGSVQWRFVMAADHPLA
CHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHCCCCCCCCCEEEEEEECCCCCC
NVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQKEIIVPDMETKIAAHLAGVGIGF
CHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHCCHHH
LPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKFGSGKAVEDIVTLFTQRRPEISGFL
HHHHHHHHHHCCHHHHHHHHCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHH
EIFGNPRS
HHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]