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The map label for this gene is sbcD

Identifier: 157159915

GI number: 157159915

Start: 479542

End: 480744

Strand: Reverse

Name: sbcD

Synonym: EcHS_A0466

Alternate gene names: 157159915

Gene position: 480744-479542 (Counterclockwise)

Preceding gene: 157159919

Following gene: 157159914

Centisome position: 10.35

GC content: 53.12

Gene sequence:

>1203_bases
ATGCGCATCCTTCACACCTCAGACTGGCATCTCGGCCAGAACTTCTACAGTAAAAGCCGCGAAGCTGAACATCAGGCTTT
TCTTGACTGGCTGCTGGAAACAGCACAAACCCATCAGGTGGATGCGATTATTGTTGCTGGTGATGTTTTCGATACCGGCT
CGCCGCCCAGTTACGCCCGCACGTTATACAACCGTTTTGTTGTCAATTTACAGCAAACTGGCTGTCATCTGGTGGTACTG
GCAGGAAACCATGACTCGGTCGCCACGCTGAATGAATCGCGCGATATCATGGCGTTCCTCAATACGACCGTGGTCGCCAG
CGCCGGACATGCGCCGCAAATCTTGCCTCGTCGCGACGGGACGCCAGGCGCAGTGCTGTGCCCCATTCCGTTTTTACGTC
CGCGTGACATTATTACCAGCCAGGCGGGGCTTAACGGTATTGAAAAACAGCAGCATTTACTGGCAGCGATTACCGATTAT
TACCAACAACACTATGCCGATGCCTGCAAACTGCGCGGCGATCAGCCTCTGCCCATCATCGCCACGGGACACTTAACGAC
CGTGGGTGCCAGTAAAAGTGACGCCGTGCGTGACATTTATATTGGCACGCTGGACGCGTTTCCAGCACAAAACTTTCCAC
CAGCCGACTACATCGCGCTCGGGCATATTCACCGCGCACAGATTATTGGCGGCATGGAACATGTTCGCTATTGCGGCTCT
CCCATTCCACTGAGTTTTGATGAATGCGGAAAGAGTAAATATGTCCATCTGGTGACATTTTCAAACGGCAAATTAGAGAG
CGTGGAAAACCTGAACGTACCGGTAACGCAACCCATGGCGGTGCTGAAAGGCGATCTGGCGTCGATTACCGCACAGCTGG
AACAGTGGCGCGATGTATCACAGGAGCCGCCTGTCTGGCTGGATATCGAAATCACTACTGATGAGTATCTGCATGATATT
CAGCGCAAAATCCAGGCATTAACCGAATCATTGCCTGTTGAAGTATTGCTGGTACGCCGGAGTCGTGAACAGCGCGAGCG
TGTGTTAGCCAGCCAACAGCGTGAAACCCTCAGCGAACTCAGCGTCGAAGAGGTGTTCAATCGCCGTCTGGCACTGGAAG
AACTGGATGAATCGCAGCAGCAACGTCTGCAGCATCTTTTCACCACGACGTTGCATACCCTCGCCGGAGAACACGAAGCA
TGA

Upstream 100 bases:

>100_bases
CACATATCTTCAGGTTATTGATTTCCGTGGCGCAGAAAAAAGCAAATGGCACATCTGTTTGGGTATAATCGCGCCCATGC
TTTTTCGCCAGGGAATCGTT

Downstream 100 bases:

>100_bases
AAATTCTCAGCCTGCGCCTGAAAAACCTGAACTCATTAAAAGGCGAATGGAAGATTGATTTCACCCGCGAGCCGTTCGCC
AGCAACGGGCTGTTTGCCAT

Product: exonuclease subunit SbcD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAIIVAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVL
AGNHDSVATLNESRDIMAFLNTTVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYIALGHIHRAQIIGGMEHVRYCGS
PIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVTQPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDI
QRKIQALTESLPVEVLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLHTLAGEHEA

Sequences:

>Translated_400_residues
MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAIIVAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVL
AGNHDSVATLNESRDIMAFLNTTVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYIALGHIHRAQIIGGMEHVRYCGS
PIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVTQPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDI
QRKIQALTESLPVEVLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLHTLAGEHEA
>Mature_400_residues
MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAIIVAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVL
AGNHDSVATLNESRDIMAFLNTTVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYIALGHIHRAQIIGGMEHVRYCGS
PIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVTQPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDI
QRKIQALTESLPVEVLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLHTLAGEHEA

Specific function: SbcCD cleaves DNA hairpin structures. These structures can inhibit DNA replication and are intermediates in certain DNA recombination reactions. The complex acts as a 3'->5' double strand exonuclease that can open hairpins. It also has a 5' single-strand

COG id: COG0420

COG function: function code L; DNA repair exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sbcD family

Homologues:

Organism=Escherichia coli, GI1786598, Length=400, Percent_Identity=100, Blast_Score=827, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SBCD_ECO57 (P0AG77)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   D85535
- PIR:   H90684
- RefSeq:   NP_286136.1
- RefSeq:   NP_308475.1
- ProteinModelPortal:   P0AG77
- SMR:   P0AG77
- EnsemblBacteria:   EBESCT00000027175
- EnsemblBacteria:   EBESCT00000058379
- GeneID:   914550
- GeneID:   957348
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z0496
- KEGG:   ecs:ECs0448
- GeneTree:   EBGT00050000010941
- HOGENOM:   HBG677966
- OMA:   SDWHIGK
- ProtClustDB:   PRK10966
- BioCyc:   ECOL83334:ECS0448-MONOMER
- InterPro:   IPR004843
- InterPro:   IPR004593
- TIGRFAMs:   TIGR00619

Pfam domain/function: PF00149 Metallophos

EC number: NA

Molecular weight: Translated: 44714; Mature: 44714

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAIIVAGDVFDTGSPPSYAR
CEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHH
TLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNTTVVASAGHAPQILPRRDG
HHHHHHHHEEECCCEEEEEEECCCCCEEEECCCCHHHHHHHHHHEECCCCCCCCCCCCCC
TPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDYYQQHYADACKLRGDQPLPII
CCCEEECCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
ATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYIALGHIHRAQIIGGMEHVRYCGS
EECCEEEECCCCCCCHHEEEEEECCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHCCC
PIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVTQPMAVLKGDLASITAQLEQWRDVS
CCCCCHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
QEPPVWLDIEITTDEYLHDIQRKIQALTESLPVEVLLVRRSREQRERVLASQQRETLSEL
CCCCEEEEEEECCHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHH
SVEEVFNRRLALEELDESQQQRLQHLFTTTLHTLAGEHEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAIIVAGDVFDTGSPPSYAR
CEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHH
TLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNTTVVASAGHAPQILPRRDG
HHHHHHHHEEECCCEEEEEEECCCCCEEEECCCCHHHHHHHHHHEECCCCCCCCCCCCCC
TPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDYYQQHYADACKLRGDQPLPII
CCCEEECCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
ATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYIALGHIHRAQIIGGMEHVRYCGS
EECCEEEECCCCCCCHHEEEEEECCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHCCC
PIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVTQPMAVLKGDLASITAQLEQWRDVS
CCCCCHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
QEPPVWLDIEITTDEYLHDIQRKIQALTESLPVEVLLVRRSREQRERVLASQQRETLSEL
CCCCEEEEEEECCHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHH
SVEEVFNRRLALEELDESQQQRLQHLFTTTLHTLAGEHEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796