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The map label for this gene is mak [H]

Identifier: 157159912

GI number: 157159912

Start: 474041

End: 474949

Strand: Direct

Name: mak [H]

Synonym: EcHS_A0462

Alternate gene names: 157159912

Gene position: 474041-474949 (Clockwise)

Preceding gene: 157159910

Following gene: 157159916

Centisome position: 10.21

GC content: 55.56

Gene sequence:

>909_bases
GTGCGTATAGGTATCGATTTAGGCGGCACCAAAACTGAAGTGATTGCACTGGGCGATGCAGGGGAGCAGTTGTACCGCCA
TCGTCTGCCCACGCCGCGTGATGATTACCGGCAGACTATTGAAACGATCGCCACGTTGGTTGATATGGCGGAGCAGGCGA
CAGGGCAGCGCGGAACGGTAGGTATGGGCATTCCTGGCTCAATTTCGCCTTACACCGGCGTGGTGAAAAATGCCAATTCA
ACCTGGCTCAACGGTCAGCCATTCGATAAAGATTTAAGCGCGCGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCCAA
CTGTCTGGCGGTTTCAGAAGCGGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGAT
GCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGCGAGTGGGGACACAATCCGCTA
CCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTT
TATTTCGGGCACGGGATTTGCCACAGATTATCGTCGTTTGAGCGGACATGCGCTGAAAGGCAGTGAAATTATCAGCCTGG
TTGAAGAAAGCGATCCGGTAGCGGAACTGGCATTGCGTCGCTACGAGCTGCGGCTGGCAAAATCGCTGGCACATGTCGTG
AATATTCTCGATCCGGATGTAATTGTCCTGGGGGGCGGGATGAGCAATGTAGACCGTTTATATCAAACGGTTGGGCAGTT
GATTAAACAATTTGTCTTCGGCGGCGAATGTGAAACGCCGGTGCGTAAGGCGAAGCACGGTGATTCCAGCGGCGTACGCG
GCGCTGCGTGGTTATGGCCACAAGAGTAA

Upstream 100 bases:

>100_bases
AAATTTAGCGGGCATGATAACGAATTGTCGGCGGCCTTGCATTGCCAATCCGGTTGTCCGTCTCTACGCTATTGATATTG
AAAAAAATAAGGAGAGTACC

Downstream 100 bases:

>100_bases
AAAACGTAGGCAATTGGCGCATCATGCTTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAATGTGCCGGAACCGC
GTAGGGCGGATAAGGCGTTC

Product: fructokinase

Products: NA

Alternate protein names: D-fructose kinase; Manno(fructo)kinase [H]

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIATLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANS
TWLNGQPFDKDLSARLQREVRLANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIISLVEESDPVAELALRRYELRLAKSLAHVV
NILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGECETPVRKAKHGDSSGVRGAAWLWPQE

Sequences:

>Translated_302_residues
MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIATLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANS
TWLNGQPFDKDLSARLQREVRLANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIISLVEESDPVAELALRRYELRLAKSLAHVV
NILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGECETPVRKAKHGDSSGVRGAAWLWPQE
>Mature_302_residues
MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIATLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANS
TWLNGQPFDKDLSARLQREVRLANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIISLVEESDPVAELALRRYELRLAKSLAHVV
NILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGECETPVRKAKHGDSSGVRGAAWLWPQE

Specific function: Catalyzes the phosphorylation of fructose to fructose-6- P. Has also low level glucokinase activity in vitro. Is not able to phosphorylate D-ribose, D-mannitol, D-sorbitol, inositol, and L-threonine [H]

COG id: COG1940

COG function: function code KG; Transcriptional regulator/sugar kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family [H]

Homologues:

Organism=Escherichia coli, GI87081733, Length=302, Percent_Identity=99.3377483443709, Blast_Score=616, Evalue=1e-178,
Organism=Escherichia coli, GI1787363, Length=306, Percent_Identity=40.5228758169935, Blast_Score=206, Evalue=1e-54,
Organism=Escherichia coli, GI1786891, Length=272, Percent_Identity=22.7941176470588, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1790522, Length=315, Percent_Identity=26.984126984127, Blast_Score=76, Evalue=3e-15,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000600 [H]

Pfam domain/function: PF00480 ROK [H]

EC number: =2.7.1.4 [H]

Molecular weight: Translated: 32401; Mature: 32401

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS01125 ROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIATLVDMAEQATGQRGTV
CEEEEECCCCCEEEEEECCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRLANDANCLAVSEAVDGAAA
CCCCCCCCCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEHHHHCCHHH
GAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPLPWMDEDELRYREEVPCYCGK
HHHHEEHHEEECCCCCCEEECCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCC
QGCIETFISGTGFATDYRRLSGHALKGSEIISLVEESDPVAELALRRYELRLAKSLAHVV
HHHHHHHHCCCCCHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
NILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGECETPVRKAKHGDSSGVRGAAWLWP
HHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCEEECCC
QE
CC
>Mature Secondary Structure
MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIATLVDMAEQATGQRGTV
CEEEEECCCCCEEEEEECCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRLANDANCLAVSEAVDGAAA
CCCCCCCCCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEHHHHCCHHH
GAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPLPWMDEDELRYREEVPCYCGK
HHHHEEHHEEECCCCCCEEECCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCC
QGCIETFISGTGFATDYRRLSGHALKGSEIISLVEESDPVAELALRRYELRLAKSLAHVV
HHHHHHHHCCCCCHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
NILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGECETPVRKAKHGDSSGVRGAAWLWP
HHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCEEECCC
QE
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8807285; 9278503; 1744033 [H]