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The map label for this gene is ampH

Identifier: 157159891

GI number: 157159891

Start: 459027

End: 460184

Strand: Reverse

Name: ampH

Synonym: EcHS_A0439

Alternate gene names: 157159891

Gene position: 460184-459027 (Counterclockwise)

Preceding gene: 157159892

Following gene: 157159888

Centisome position: 9.91

GC content: 55.18

Gene sequence:

>1158_bases
TTGAAACGTAGTCTGCTTTTTTCTGCCGTGCTGTGTGCGGCGTCATTGACCTCCGTCCATGCGGCGCAGCCGATCACTGA
ACCGGAGTTTGCCTCTGATATTGTCGATCGTTATGCCGATCATATTTTTTACGGCAGCGGTGCCACGGGGATGGCGCTGG
TTGTTATCGACGGTAACCAGCGCGTCTTTCGCAGTTATGGCGAAACGCGACCTGGTAATAACGTTCGCCCGCAGCTGGAT
TCGGTCGTGCGTATTGCTTCCCTCACCAAGCTAATGACCAGTGAAATGCTGGTGAAATTGCTCGACCAGGGGACCGTGAA
GTTAAACGATCCGCTAAGTAAATATGCTCCGCCAGGCGCACGCGTGCCAACCTACAATGGGACGCCCATTACCCTAGTGA
ATCTGGCAACCCATACCAGCGCCCTGCCCCGTGAACAGCCCGGTGGCGCGGCACATCGTCCGGTGTTTGTCTGGCCAACG
CGCGAGCAACGCTGGAAATACCTTTCTACGGCGAAGCTGAAAGCCGCGCCAGGCAGTCAGGCGGCGTACTCTAACCTTGC
GTTCGACCTGCTGGCAGATGCACTGGCGAATGCGTCCGGCAAGCCTTATACCCAGTTGTTTGAAGAGCAGATTACCCGTC
CATTGGGGATGAAAGACACCACCTACACCCCCTCACCGGATCAGTGCCGCCGTTTAATGGTTGCTGAGCGCGGTGCCAGT
CCGTGCAATAACACGCTGGCGGCAATTGGCAGCGGCGGCGTCTATTCCACGCCTGGCGATATGATGCGCTGGATGCAGCA
GTATTTGTCATCTGATTTTTATCAGCGCAGTAACCAGGCAGATCGCATGCAGACGTTGATTTATCAGCGCGCGCAGTTTA
CGAAAGTGATTGGCATGGATGTCCCCGGCAAAGCGGATGCGCTTGGTTTAGGCTGGGTATATATGGCCCCGAAAGAGGGT
CGTCCGGGGATTATTCAGAAGACAGGCGGTGGTGGCGGTTTCATTACCTATATGGCGATGATCCCGCAGAAGAATATTGG
CGCGTTTGTGGTGGTCACTCGTTCGCCGTTGACGCGCTTTAAAAATATGAGCGATGGCATCAATGATCTGGTGACCGAGC
TAAGCGGGAATAAACCGTTGGTTATCCCCGCATCCTGA

Upstream 100 bases:

>100_bases
CGTTTTTAACACTATTATTGCACTAATTCGCCCTTTGCAATCCATCAATGAGTAGTATAAATACGCTCAGTTACCTTCAT
TCAATCTATGGACACCACCG

Downstream 100 bases:

>100_bases
TTAATACTCTGAAGGCAAACGGTTGATAGCGACCAGTTTGCCTTTATTCATTTCGATATAGCCGCCTTTACGCAAAGCAG
CGAGAACTTCTGCGACGACA

Product: beta-lactam binding protein AmpH

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 385; Mature: 385

Protein sequence:

>385_residues
MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFYGSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLD
SVVRIASLTKLMTSEMLVKLLDQGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMKDTTYTPSPDQCRRLMVAERGAS
PCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQRSNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEG
RPGIIQKTGGGGGFITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS

Sequences:

>Translated_385_residues
MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFYGSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLD
SVVRIASLTKLMTSEMLVKLLDQGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMKDTTYTPSPDQCRRLMVAERGAS
PCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQRSNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEG
RPGIIQKTGGGGGFITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS
>Mature_385_residues
MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFYGSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLD
SVVRIASLTKLMTSEMLVKLLDQGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMKDTTYTPSPDQCRRLMVAERGAS
PCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQRSNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEG
RPGIIQKTGGGGGFITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS

Specific function: Binds penicillin and contributes to the normal morphology

COG id: COG1680

COG function: function code V; Beta-lactamase class C and other penicillin binding proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-lactamase family

Homologues:

Organism=Homo sapiens, GI239747261, Length=364, Percent_Identity=25, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI239752721, Length=364, Percent_Identity=25, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI239741085, Length=364, Percent_Identity=25, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI1786574, Length=385, Percent_Identity=100, Blast_Score=796, Evalue=0.0,
Organism=Escherichia coli, GI1790593, Length=372, Percent_Identity=23.6559139784946, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI87082101, Length=245, Percent_Identity=26.1224489795918, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AMPH_ECO57 (P0AD71)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   B90682
- PIR:   F85532
- RefSeq:   NP_286114.1
- RefSeq:   NP_308453.1
- ProteinModelPortal:   P0AD71
- EnsemblBacteria:   EBESCT00000026485
- EnsemblBacteria:   EBESCT00000057395
- GeneID:   914528
- GeneID:   957318
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z0472
- KEGG:   ecs:ECs0426
- GeneTree:   EBGT00050000010224
- HOGENOM:   HBG416564
- OMA:   DMQRWMQ
- ProtClustDB:   PRK10662
- BioCyc:   ECOL83334:ECS0426-MONOMER
- InterPro:   IPR012338
- InterPro:   IPR001466

Pfam domain/function: PF00144 Beta-lactamase; SSF56601 PBP_transp_fold

EC number: NA

Molecular weight: Translated: 41850; Mature: 41850

Theoretical pI: Translated: 9.85; Mature: 9.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFYGSGATGMALVVIDGNQ
CCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHEEECCCCCCEEEEEECCCH
RVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLDQGTVKLNDPLSKYAPPGA
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHCCCCC
RVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPTREQRWKYLSTAKLKAAPGSQ
CCCCCCCCCEEEEEECHHHCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHEEECCCCCH
AAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMKDTTYTPSPDQCRRLMVAERGAS
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCC
PCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQRSNQADRMQTLIYQRAQFTKVIGMD
CCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCC
VPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGFITYMAMIPQKNIGAFVVVTRSPLTRF
CCCCCCCEEEEEEEECCCCCCCCEEEECCCCCCCEEHEECCCCCCCCEEEEEECCHHHHH
KNMSDGINDLVTELSGNKPLVIPAS
HHHCCCHHHHHHHHCCCCCEEEECC
>Mature Secondary Structure
MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFYGSGATGMALVVIDGNQ
CCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHEEECCCCCCEEEEEECCCH
RVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLDQGTVKLNDPLSKYAPPGA
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHCCCCC
RVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPTREQRWKYLSTAKLKAAPGSQ
CCCCCCCCCEEEEEECHHHCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHEEECCCCCH
AAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMKDTTYTPSPDQCRRLMVAERGAS
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCC
PCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQRSNQADRMQTLIYQRAQFTKVIGMD
CCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCC
VPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGFITYMAMIPQKNIGAFVVVTRSPLTRF
CCCCCCCEEEEEEEECCCCCCCCEEEECCCCCCCEEHEECCCCCCCCEEEEEECCHHHHH
KNMSDGINDLVTELSGNKPLVIPAS
HHHCCCHHHHHHHHCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796