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The map label for this gene is mhpB

Identifier: 157159864

GI number: 157159864

Start: 434783

End: 435727

Strand: Direct

Name: mhpB

Synonym: EcHS_A0412

Alternate gene names: 157159864

Gene position: 434783-435727 (Clockwise)

Preceding gene: 157159863

Following gene: 157159865

Centisome position: 9.36

GC content: 57.35

Gene sequence:

>945_bases
ATGCACGCTTATCTTCACTGTCTTTCCCACTCGCCGCTGGTGGGGTATGTCGACCCGGCGCAAGAGGTGCTCGATGAGGT
CAATGGCGTGATTGCCAGCGCCCGCGAGCGTATTGCGGCATTCTCCCCTGAACTGGTGGTGCTGTTTGCGCCAGATCACT
ACAACGGCTTTTTCTATGACGTGATGCCACCGTTCTGTTTAGGCGTTGGAGCGACGGCAATTGGTGATTTCGGCAGTGCG
GCAGGAGAGCTGCCCGTGCCTGTGGAGCTGGCGGAGGCCTGTGCGCATGCTGTCATGAAGAGCGGGATCGATCTTGCCGT
TTCTTACTGTATGCAGGTGGACCACGGGTTCGCCCAGCCGCTGGAGTTCCTGCTCGGTGGGCTGGATAAGGTGCCAGTTC
TGCCTGTGTTCATCAACGGTGTCGCCACGCCGCTGCCCGGTTTCCAGCGTACCCGCATGTTGGGTGAAGCCATTGGACGT
TTCACCAGCACTCTCAATAAACGCGTGCTGTTCCTGGGTTCCGGTGGGCTTTCCCATCAGCCGCCGGTGCCCGAACTGGC
GAAAGCCGATGCCCATATGCGCGACCGTCTGTTGGGGAGCGGGAAAGATTTACCCGCCAGTGAGCGCGAATTGCGTCAGC
AACGGGTGATTAGCGCCGCTGAGAAGTTTGTTGAGGATCAGAGAACTCTGCATCCGCTCAACCCGATTTGGGATAACCAG
TTCATGACTTTGCTGGAGCAGGGACGCATACAGGAACTGGATGCCGTCAGTAACGAAGAGCTTTCCGCCATTGCCGGAAA
GTCGACACATGAAATCAAAACCTGGGTCGCCGCTTTTGCCGCTATTTCTACATTTGGCAACTGGCGTAGCGAAGGGCGTT
ATTACCGCCCAATCCCGGAGTGGATTGCCGGATTTGGCTCGTTAAGCGCCAGAACAGAGAACTGA

Upstream 100 bases:

>100_bases
GCCATTCCGCAAACCCTGGGCAAGACCCTGAATAAACTGGCGTCGGTGATGACGCTGACCCGCCCTGATGCCGACGTTTC
TGTCGAAAAGGTAGCCTGAT

Downstream 100 bases:

>100_bases
ATATGCAGGAGAAGATGATGAGTTATCAGCCACAAACCGAAGCCGCCACCAGCCGTTTTCTGAATGTAGAAGAAGCGGGT
AAAACGCTGCGCATCCATTT

Product: 3-(2,3-dihydroxyphenyl)propionate dioxygenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPELVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSA
AGELPVPVELAEACAHAVMKSGIDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVISAAEKFVEDQRTLHPLNPIWDNQ
FMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWVAAFAAISTFGNWRSEGRYYRPIPEWIAGFGSLSARTEN

Sequences:

>Translated_314_residues
MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPELVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSA
AGELPVPVELAEACAHAVMKSGIDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVISAAEKFVEDQRTLHPLNPIWDNQ
FMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWVAAFAAISTFGNWRSEGRYYRPIPEWIAGFGSLSARTEN
>Mature_314_residues
MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPELVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSA
AGELPVPVELAEACAHAVMKSGIDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVISAAEKFVEDQRTLHPLNPIWDNQ
FMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWVAAFAAISTFGNWRSEGRYYRPIPEWIAGFGSLSARTEN

Specific function: Catalyzes the non-heme iron(II)-dependent oxidative cleavage of 2,3-dihydroxyphenylpropionic acid and 2,3- dihydroxicinnamic acid into 2-hydroxy-6-ketononadienedioate and 2- hydroxy-6-ketononatrienedioate, respectively

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ligB/mhpB extradiol dioxygenase family

Homologues:

Organism=Escherichia coli, GI1786544, Length=314, Percent_Identity=99.6815286624204, Blast_Score=644, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MHPB_ECO24 (A7ZI95)

Other databases:

- EMBL:   CP000800
- RefSeq:   YP_001461524.1
- ProteinModelPortal:   A7ZI95
- STRING:   A7ZI95
- EnsemblBacteria:   EBESCT00000020664
- GeneID:   5589309
- GenomeReviews:   CP000800_GR
- KEGG:   ecw:EcE24377A_0372
- eggNOG:   NOG42398
- GeneTree:   EBGT00050000010475
- HOGENOM:   HBG565586
- OMA:   PLNPEWD
- ProtClustDB:   PRK13370
- BioCyc:   ECOL331111:ECE24377A_0372-MONOMER
- HAMAP:   MF_01653
- InterPro:   IPR004183

Pfam domain/function: PF02900 LigB; SSF53213 Xdiol_dOase_3B

EC number: =1.13.11.16

Molecular weight: Translated: 34226; Mature: 34226

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: NA

Important sites: ACT_SITE 115-115 ACT_SITE 179-179

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPELVVLFAPDHYNGFFYD
CCHHHHHHCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEE
VMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSGIDLAVSYCMQVDHGFAQP
CCCHHHHHCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHH
LEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGRFTSTLNKRVLFLGSGGLSHQ
HHHHHCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCC
PPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVISAAEKFVEDQRTLHPLNPIWDNQ
CCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
FMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWVAAFAAISTFGNWRSEGRYYRPIPE
HHHHHHHCCHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCEECCCHH
WIAGFGSLSARTEN
HHHHHCCCCCCCCC
>Mature Secondary Structure
MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPELVVLFAPDHYNGFFYD
CCHHHHHHCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEE
VMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSGIDLAVSYCMQVDHGFAQP
CCCHHHHHCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHH
LEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGRFTSTLNKRVLFLGSGGLSHQ
HHHHHCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCC
PPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVISAAEKFVEDQRTLHPLNPIWDNQ
CCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
FMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWVAAFAAISTFGNWRSEGRYYRPIPE
HHHHHHHCCHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCEECCCHH
WIAGFGSLSARTEN
HHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA