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The map label for this gene is mhpA

Identifier: 157159863

GI number: 157159863

Start: 433117

End: 434781

Strand: Direct

Name: mhpA

Synonym: EcHS_A0411

Alternate gene names: 157159863

Gene position: 433117-434781 (Clockwise)

Preceding gene: 157159858

Following gene: 157159864

Centisome position: 9.33

GC content: 56.82

Gene sequence:

>1665_bases
ATGGCAATACAACACCCTGACATCCAGCCTGCTGTTAACCATAGCGTTCAGGTGGCGATCGCTGGTGCCGGTCCGGTTGG
GCTGATGATGGCGAACTATCTCGGTCAGATGGGCATTGACGTGCTGGTGGTGGAGAAACTCGATAAGTTGATCGACTACC
CGCGTGCGATTGGTATTGATGACGAGGCGCTGCGCACCATGCAGTCGGTCGGCCTGGTCGAGAATGTTCTGCCGCACACT
ACACCGTGGCACGCGATGCGTTTTCTCACCCCAAAAGGCCGCTGTTTTGCTGATATTCAGCCAATGACCGATGAATTTGG
CTGGCCGCGCCGTAACGCCTTTATTCAGCCACAGGTCGATGCGGTGATGCTGGAAGGGTTGTCGCGTTTTCCGAATGTGC
GCTGCTTGTTTGCCCGCGAGCTGGAGGCCTTCAGCCAGCAAAATGACGAAGTGACCTTGCACCTGAAAACGGCAGAAGGG
CAGCGGGAAACGGTCAAAGCCCAGTGGCTGGTAGCCTGTGACGGTGGAGCAAGTTTTGTCCGTCGCACTCTGAATGTGCC
GTTTGAAGGTAAAACTGCGCCAAATCAGTGGATTGTGGTAGATATCGCCAACGATCCGTTAAGTACGCCGCATATCTATT
TGTGTTGCGATCCGGTGCGCCCGTATGTTTCTGCCGCGCTGCCTCATGCGGTACGTCGCTTTGAATTTATGGTGATGCCG
GGAGAAACCGAAGAGCAGCTGCGTGAGCCGCAAAATATGCGCAAGCTGTTAAGCAAAGTGCTGCCTAATCCGGACAATGT
TGAATTGATTCGCCAGCGTGTCTACACCCACAACGCGCGACTGGCGCAACGTTTCCGTATTGATCGCGTACTGCTGGCGG
GCGATGCCGCGCACATCATGCCGGTATGGCAGGGGCAGGGCTATAACAGTGGTATGCGCGACGCCTTTAACCTCGCATGG
AAACTGGCGTTGGTTATCCAGGGGAAAGCCCGCGATGCGCTGCTCGATACCTATCAACAAGAACGTCGCGATCACGCCAA
AGCGATGATTGACCTGTCCGTGACGGCGGGCAACGTGCTGGCTCCGCCGAAACGCTGGCAGGGTACGTTACGTGACGGCG
TTTCCTGGCTGTTGAATTATCTGCCGCCAGTAAAACGCTACTTCCTCGAAATGCGCTTCAAGCCGATGCCGCAATATTAC
GGCGGTGCGCTGATGCGTGAGGGCGAAGCGAAGCACTCTCCGGTCGGCAAGATGTTTATTCAGCCGAAAGTCACGCTGGA
AAACGGCGACGTGACGCTGCTCGATAACGCGATCGGCGCGAACTTCGCGGTAATTGGCTGGGGATGCAATCCACTGTGGG
GGATGAGCGACGAGCAAATCCAGCAGTGGCGCGCGTTGGGCACACGCTTCATTCAGGTGGTGCCGGAAGTGCAAATTCAT
ACCGCACAGGATAACCACGACGGCGTACTACGCGTGGGCGATACGCAAGGTCGCCTGCGTAGCTGGTTCGCGCAACACAA
TGCTTCGCTGGTGGTGATGCGCCCGGATCGCTTTGTTGCCGCCACCGCCATTCCGCAAACCCTGGGCAAGACCCTGAATA
AACTGGCGTCGGTGATGACGCTGACCCGCCCTGATGCCGACGTTTCTGTCGAAAAGGTAGCCTGA

Upstream 100 bases:

>100_bases
ACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGCTGTACTGTGCGCGCAACGGCATTTTGT
CCGAGTCGTGAGGTACTGAA

Downstream 100 bases:

>100_bases
TATGCACGCTTATCTTCACTGTCTTTCCCACTCGCCGCTGGTGGGGTATGTCGACCCGGCGCAAGAGGTGCTCGATGAGG
TCAATGGCGTGATTGCCAGC

Product: 3-(3-hydroxyphenyl)propionate hydroxylase

Products: NA

Alternate protein names: 3-HCI hydroxylase; 3-HPP hydroxylase

Number of amino acids: Translated: 554; Mature: 553

Protein sequence:

>554_residues
MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVVEKLDKLIDYPRAIGIDDEALRTMQSVGLVENVLPHT
TPWHAMRFLTPKGRCFADIQPMTDEFGWPRRNAFIQPQVDAVMLEGLSRFPNVRCLFARELEAFSQQNDEVTLHLKTAEG
QRETVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDPVRPYVSAALPHAVRRFEFMVMP
GETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYTHNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAW
KLALVIQGKARDALLDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMRFKPMPQYY
GGALMREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCNPLWGMSDEQIQQWRALGTRFIQVVPEVQIH
TAQDNHDGVLRVGDTQGRLRSWFAQHNASLVVMRPDRFVAATAIPQTLGKTLNKLASVMTLTRPDADVSVEKVA

Sequences:

>Translated_554_residues
MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVVEKLDKLIDYPRAIGIDDEALRTMQSVGLVENVLPHT
TPWHAMRFLTPKGRCFADIQPMTDEFGWPRRNAFIQPQVDAVMLEGLSRFPNVRCLFARELEAFSQQNDEVTLHLKTAEG
QRETVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDPVRPYVSAALPHAVRRFEFMVMP
GETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYTHNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAW
KLALVIQGKARDALLDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMRFKPMPQYY
GGALMREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCNPLWGMSDEQIQQWRALGTRFIQVVPEVQIH
TAQDNHDGVLRVGDTQGRLRSWFAQHNASLVVMRPDRFVAATAIPQTLGKTLNKLASVMTLTRPDADVSVEKVA
>Mature_553_residues
AIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVVEKLDKLIDYPRAIGIDDEALRTMQSVGLVENVLPHTT
PWHAMRFLTPKGRCFADIQPMTDEFGWPRRNAFIQPQVDAVMLEGLSRFPNVRCLFARELEAFSQQNDEVTLHLKTAEGQ
RETVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDPVRPYVSAALPHAVRRFEFMVMPG
ETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYTHNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWK
LALVIQGKARDALLDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMRFKPMPQYYG
GALMREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCNPLWGMSDEQIQQWRALGTRFIQVVPEVQIHT
AQDNHDGVLRVGDTQGRLRSWFAQHNASLVVMRPDRFVAATAIPQTLGKTLNKLASVMTLTRPDADVSVEKVA

Specific function: Catalyzes the insertion of one atom of molecular oxygen into position 2 of the phenyl ring of 3-(3- hydroxyphenyl)propionate (3-HPP) and hydroxycinnamic acid (3HCI)

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pheA/tfdB FAD monooxygenase family

Homologues:

Organism=Escherichia coli, GI1786543, Length=554, Percent_Identity=98.9169675090253, Blast_Score=1137, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MHPA_ECO24 (A7ZI94)

Other databases:

- EMBL:   CP000800
- RefSeq:   YP_001461523.1
- ProteinModelPortal:   A7ZI94
- SMR:   A7ZI94
- STRING:   A7ZI94
- EnsemblBacteria:   EBESCT00000018928
- GeneID:   5589374
- GenomeReviews:   CP000800_GR
- KEGG:   ecw:EcE24377A_0371
- eggNOG:   COG0654
- GeneTree:   EBGT00050000008990
- HOGENOM:   HBG565573
- OMA:   TTSSTRW
- ProtClustDB:   PRK06183
- BioCyc:   ECOL331111:ECE24377A_0371-MONOMER
- HAMAP:   MF_01652
- InterPro:   IPR002938
- InterPro:   IPR003042
- PRINTS:   PR00420

Pfam domain/function: PF01494 FAD_binding_3

EC number: 1.14.13.-

Molecular weight: Translated: 62185; Mature: 62053

Theoretical pI: Translated: 8.37; Mature: 8.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVVEKLDKLIDYPRAIGID
CCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCC
DEALRTMQSVGLVENVLPHTTPWHAMRFLTPKGRCFADIQPMTDEFGWPRRNAFIQPQVD
HHHHHHHHHHHHHHHHCCCCCCHHHHEEECCCCCEEEECCCCHHHCCCCCCCCCCCCCHH
AVMLEGLSRFPNVRCLFARELEAFSQQNDEVTLHLKTAEGQRETVKAQWLVACDGGASFV
HHHHHHHHHCCCEEEEHHHHHHHHHCCCCEEEEEEEECCCCCCEEEEEEEEEECCCHHHH
RRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDPVRPYVSAALPHAVRRFEFMVMP
HHHHCCCCCCCCCCCCEEEEEECCCCCCCCEEEEEECCCCHHHHHHHHHHHHHEEEEECC
GETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYTHNARLAQRFRIDRVLLAGDAAHIM
CCCHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCEEEEECCCHHEE
PVWQGQGYNSGMRDAFNLAWKLALVIQGKARDALLDTYQQERRDHAKAMIDLSVTAGNVL
EEECCCCCCCCHHHHHHHHEEEEEEEECCCHHHHHHHHHHHHHHHHHEEEEEEEECCCCC
APPKRWQGTLRDGVSWLLNYLPPVKRYFLEMRFKPMPQYYGGALMREGEAKHSPVGKMFI
CCCHHHCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHCCHHEECCCCCCCCCCEEEE
QPKVTLENGDVTLLDNAIGANFAVIGWGCNPLWGMSDEQIQQWRALGTRFIQVVPEVQIH
CCEEEEECCCEEEEECCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEE
TAQDNHDGVLRVGDTQGRLRSWFAQHNASLVVMRPDRFVAATAIPQTLGKTLNKLASVMT
ECCCCCCCEEEECCCHHHHHHHHHHCCCEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHH
LTRPDADVSVEKVA
CCCCCCCCCHHCCC
>Mature Secondary Structure 
AIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVVEKLDKLIDYPRAIGID
CCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCC
DEALRTMQSVGLVENVLPHTTPWHAMRFLTPKGRCFADIQPMTDEFGWPRRNAFIQPQVD
HHHHHHHHHHHHHHHHCCCCCCHHHHEEECCCCCEEEECCCCHHHCCCCCCCCCCCCCHH
AVMLEGLSRFPNVRCLFARELEAFSQQNDEVTLHLKTAEGQRETVKAQWLVACDGGASFV
HHHHHHHHHCCCEEEEHHHHHHHHHCCCCEEEEEEEECCCCCCEEEEEEEEEECCCHHHH
RRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDPVRPYVSAALPHAVRRFEFMVMP
HHHHCCCCCCCCCCCCEEEEEECCCCCCCCEEEEEECCCCHHHHHHHHHHHHHEEEEECC
GETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYTHNARLAQRFRIDRVLLAGDAAHIM
CCCHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCEEEEECCCHHEE
PVWQGQGYNSGMRDAFNLAWKLALVIQGKARDALLDTYQQERRDHAKAMIDLSVTAGNVL
EEECCCCCCCCHHHHHHHHEEEEEEEECCCHHHHHHHHHHHHHHHHHEEEEEEEECCCCC
APPKRWQGTLRDGVSWLLNYLPPVKRYFLEMRFKPMPQYYGGALMREGEAKHSPVGKMFI
CCCHHHCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHCCHHEECCCCCCCCCCEEEE
QPKVTLENGDVTLLDNAIGANFAVIGWGCNPLWGMSDEQIQQWRALGTRFIQVVPEVQIH
CCEEEEECCCEEEEECCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEE
TAQDNHDGVLRVGDTQGRLRSWFAQHNASLVVMRPDRFVAATAIPQTLGKTLNKLASVMT
ECCCCCCCEEEECCCHHHHHHHHHHCCCEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHH
LTRPDADVSVEKVA
CCCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA