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The map label for this gene is cynX [H]

Identifier: 157159858

GI number: 157159858

Start: 425199

End: 426353

Strand: Direct

Name: cynX [H]

Synonym: EcHS_A0406

Alternate gene names: 157159858

Gene position: 425199-426353 (Clockwise)

Preceding gene: 157159857

Following gene: 157159863

Centisome position: 9.16

GC content: 58.44

Gene sequence:

>1155_bases
ATGCTGCTGGTTCTGGTGCTGATTGGTCTTAATATGCGACCACTGCTCACCTCCGTCGGGCCACTGCTACCGCAATTGCG
CCAGGCGAGCGGAATGAGCTTTAGCGTGGCTGCCCTGTTGACCGCTCTTCCGGTGGTCACCATGGGCGGGCTGGCGCTGG
CCGGAAGCTGGCTTCATCAGCATGTCAGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTG
ATGCGTGAGCTTTACCCCCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTCGGTGGGGTGGGGATCGGCATCATTCAGGC
GGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGCCACTGGTGATGGGGCTGTGGTCCGCGGCTCTGATGG
GCGGCGGTGGACTTGGTGCCGCCATAACGCCCTGGTTAGTTCAACATAGCGAAACCTGGTATCAAACACTCGCCTGGTGG
GCGCTGCCTGCCGTTGTTGCGCTCTTTGCCTGGTGGTGGCAAAGCGCCCGCGAGGTCGCCTCTTCCCACAAGACAACAAC
CACTCCGGTTCGCGTGGTATTTACTCCCCGCGCCTGGACGCTGGGCCTATACTTCGGTCTGATTAACGGTGGTTACGCCA
GCCTGATTGCCTGGTTACCCGCTTTCTATATTGAGATTGGTGCCAGCGCGCAGTACAGCGGTTCCTTGCTGGCATTGATG
ACGCTTGGGCAAGCCGCAGGAGCTTTGCTGATGCCTGCTATGGCTCGCCATCAGGATCGGCGCAAACTTTTGATGCTGGC
GCTGGCGTTACAACTGGTGGGTTTCTGCGGCTTTATCTGGCTGCCGCTGCAATTGCCGGTATTGTGGGCGATGGTGTGTG
GGTTAGGTCTGGGTGGCGCATTTCCGCTCTGTTTGCTGCTGGCGCTCGATCACTCTGCGCAACCTGCTATTGCTGGCAAG
CTGGTGGCGTTTATGCAGGGGATCGGTTTTATCATCGCCGGGCTTGCCCCGTGGTTTTCTGGTTTGCTGCGTAGTATCAG
CGGCAATTACCTGATGGACTGGGCATTTCATACGCTGTGCGTCGTTGGGCTGATGATCATAACCCTGCGTTTTGCACCAG
CACGTTTTCCGCAGCTGTGGGTCAAAGAGGTATGA

Upstream 100 bases:

>100_bases
ACCCGGAAGGTGGCGAACGTGCGGTCATTACCTTAGATGGTAAATATCTGCCGACCAAGCCGTTCTGACAGCCATGCGCA
ATCATCAAAAGACGTTCACG

Downstream 100 bases:

>100_bases
TGCGACGCTTGTTTCGCCAGATGATGGAGCTATAAATAATTGTCGGATGTGCCCAGTGGCGCATCCGACATTAATTGCTT
AAGCGACTTCATTCACCTGA

Product: putative cyanate transporter

Products: Proton [Cytoplasm]; Cyanate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 384; Mature: 384

Protein sequence:

>384_residues
MLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFSVAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGAL
MRELYPQSALLLSSALLGGVGIGIIQAVMPSVIKRRFQQRTPLVMGLWSAALMGGGGLGAAITPWLVQHSETWYQTLAWW
ALPAVVALFAWWWQSAREVASSHKTTTTPVRVVFTPRAWTLGLYFGLINGGYASLIAWLPAFYIEIGASAQYSGSLLALM
TLGQAAGALLMPAMARHQDRRKLLMLALALQLVGFCGFIWLPLQLPVLWAMVCGLGLGGAFPLCLLLALDHSAQPAIAGK
LVAFMQGIGFIIAGLAPWFSGLLRSISGNYLMDWAFHTLCVVGLMIITLRFAPARFPQLWVKEV

Sequences:

>Translated_384_residues
MLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFSVAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGAL
MRELYPQSALLLSSALLGGVGIGIIQAVMPSVIKRRFQQRTPLVMGLWSAALMGGGGLGAAITPWLVQHSETWYQTLAWW
ALPAVVALFAWWWQSAREVASSHKTTTTPVRVVFTPRAWTLGLYFGLINGGYASLIAWLPAFYIEIGASAQYSGSLLALM
TLGQAAGALLMPAMARHQDRRKLLMLALALQLVGFCGFIWLPLQLPVLWAMVCGLGLGGAFPLCLLLALDHSAQPAIAGK
LVAFMQGIGFIIAGLAPWFSGLLRSISGNYLMDWAFHTLCVVGLMIITLRFAPARFPQLWVKEV
>Mature_384_residues
MLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFSVAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGAL
MRELYPQSALLLSSALLGGVGIGIIQAVMPSVIKRRFQQRTPLVMGLWSAALMGGGGLGAAITPWLVQHSETWYQTLAWW
ALPAVVALFAWWWQSAREVASSHKTTTTPVRVVFTPRAWTLGLYFGLINGGYASLIAWLPAFYIEIGASAQYSGSLLALM
TLGQAAGALLMPAMARHQDRRKLLMLALALQLVGFCGFIWLPLQLPVLWAMVCGLGLGGAFPLCLLLALDHSAQPAIAGK
LVAFMQGIGFIIAGLAPWFSGLLRSISGNYLMDWAFHTLCVVGLMIITLRFAPARFPQLWVKEV

Specific function: This protein is part of an active transport system that transports exogenous cyanate into E.coli cells [H]

COG id: COG2807

COG function: function code P; Cyanate permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Cyanate porter (TC 2.A.1.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786536, Length=383, Percent_Identity=98.1723237597911, Blast_Score=732, Evalue=0.0,
Organism=Escherichia coli, GI1788092, Length=349, Percent_Identity=26.9340974212034, Blast_Score=96, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004747
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 41345; Mature: 41345

Theoretical pI: Translated: 10.69; Mature: 10.69

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFSVAALLTALPVVTMGGLALAGSWLHQ
CHHEEEHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
HVSERRSVAISLLLIAVGALMRELYPQSALLLSSALLGGVGIGIIQAVMPSVIKRRFQQR
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
TPLVMGLWSAALMGGGGLGAAITPWLVQHSETWYQTLAWWALPAVVALFAWWWQSAREVA
CCHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SSHKTTTTPVRVVFTPRAWTLGLYFGLINGGYASLIAWLPAFYIEIGASAQYSGSLLALM
HCCCCCCCCEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
TLGQAAGALLMPAMARHQDRRKLLMLALALQLVGFCGFIWLPLQLPVLWAMVCGLGLGGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
FPLCLLLALDHSAQPAIAGKLVAFMQGIGFIIAGLAPWFSGLLRSISGNYLMDWAFHTLC
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
VVGLMIITLRFAPARFPQLWVKEV
HHHHHHHHHHHCCCCCHHHHHHCC
>Mature Secondary Structure
MLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFSVAALLTALPVVTMGGLALAGSWLHQ
CHHEEEHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
HVSERRSVAISLLLIAVGALMRELYPQSALLLSSALLGGVGIGIIQAVMPSVIKRRFQQR
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
TPLVMGLWSAALMGGGGLGAAITPWLVQHSETWYQTLAWWALPAVVALFAWWWQSAREVA
CCHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SSHKTTTTPVRVVFTPRAWTLGLYFGLINGGYASLIAWLPAFYIEIGASAQYSGSLLALM
HCCCCCCCCEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
TLGQAAGALLMPAMARHQDRRKLLMLALALQLVGFCGFIWLPLQLPVLWAMVCGLGLGGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
FPLCLLLALDHSAQPAIAGKLVAFMQGIGFIIAGLAPWFSGLLRSISGNYLMDWAFHTLC
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
VVGLMIITLRFAPARFPQLWVKEV
HHHHHHHHHHHCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; Cyanate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + Cyanate [Periplasm] = Proton [Cytoplasm] + Cyanate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3049588; 9278503 [H]