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The map label for this gene is prpE [H]

Identifier: 157159852

GI number: 157159852

Start: 418302

End: 420188

Strand: Direct

Name: prpE [H]

Synonym: EcHS_A0400

Alternate gene names: 157159852

Gene position: 418302-420188 (Clockwise)

Preceding gene: 157159851

Following gene: 157159853

Centisome position: 9.01

GC content: 58.13

Gene sequence:

>1887_bases
ATGTCTTTTAGCGAATTTTATCAGCGTTCGATTAACGAACCGGAGCAGTTCTGGGCCGAGCAGGCCCGGCGTATTGACTG
GCAGACGCCCTTTACGCAAACGCTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAACTTGT
GCCACAACGCTATCGACCGCTGGCTGGAGAAACAACCAGAGGCGCTGGCGCTGATTGCCGTCTCTTCGGAAACAGAAGAA
GAGCGCACCTTTACCTTTCGTCAGCTTCATGACGAAGTAAACGCGGTGGCGTCAATGCTGCGCTCGCTGGGCGTGCAGCG
TGGCGATCGGGTGCTGGTGTATATGCCGATGATTGCCGAAGCGCATATTACTCTGCTGGCCTGCGCGCGCATTGGCGCTA
TTCATTCGGTGGTGTTTGGGGGATTTGCCTCGCACAGCGTGGCGGCGCGAATTGATGATGCTAAACCGGTGCTGATTGTC
TCGGCTGATGCCGGAGCGCGCGGTGGCAAAATCATTCCCTATAAAAAATTGCTCGACGATGCGATAAGTCAGGCGCAGCA
CCAGCCACGCCATGTTTTGCTGGTGGATCGCGGGCTGGCGAAAATGGCACGCGTCAGCGGGCGGGATGTCGATTTCGTGT
CGTTGCGCCATCAACACATCGGCGCGCGGGTACCGGTGGCGTGGCTGGAATCCAACGAAACCTCCTGCATTCTCTACACC
TCCGGCACGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCAGATATGCGGTGGCGCTGGCGACCTCGATGGACAC
CATTTTTGGCGGCAAAGCGGGCGGTGTGTTCTTTTGTGCTTCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTCT
ACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTACCGACCTGGCCGGACTGCGGCGTGTGGTGGAAA
ATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGA
AATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGG
TGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGC
GGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCGGTGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAATGAAGT
CACCGGCGAACCGTGTGGCGTCAACGAGAAAGGGATGCTGGTGGTGGAAGGGCCGCTGCCGCCGGGGTGTATTCAGACCA
TCTGGGGCGACGACGGCCGCTTTGTGAAGACTTACTGGTCGCTGTTTTCCCGCCCGGTGTACGCCACCTTTGACTGGGGC
ATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGAC
GCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAG
GGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAG
GCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAA
AACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGGGATCTGACGACCATTG
ATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAA

Upstream 100 bases:

>100_bases
CGACAGAACTCGCCTGGAACAGATGCCGGTCAATGAGTATCTCGACCTGTACGTCATTTAAGCAAACAGCGGTAAGGCGT
AAGTTCAACAGGAGAGCATT

Downstream 100 bases:

>100_bases
GGTATTGTCGGATGCGTCGCGTGGCGCATCCGACACCATGCGCCGATGCCTGATGCGACGCTTACCGCGTCTTATTATGC
CTACCCACTGTTTTTACACC

Product: propionyl-CoA synthetase

Products: NA

Alternate protein names: Propionyl-CoA synthetase [H]

Number of amino acids: Translated: 628; Mature: 627

Protein sequence:

>628_residues
MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFARWFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEE
ERTFTFRQLHDEVNAVASMLRSLGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFVSLRHQHIGARVPVAWLESNETSCILYT
SGTTGKPKGVQRDVGRYAVALATSMDTIFGGKAGGVFFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWK
IVEKYQVSRMFSAPTAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESGWPIMAIAR
GLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGCIQTIWGDDGRFVKTYWSLFSRPVYATFDWG
IRDADGYHFILGRTDDVINVAGHRLGTREIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDRDVAHSQEK
AIMALVDSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQAMEE

Sequences:

>Translated_628_residues
MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFARWFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEE
ERTFTFRQLHDEVNAVASMLRSLGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFVSLRHQHIGARVPVAWLESNETSCILYT
SGTTGKPKGVQRDVGRYAVALATSMDTIFGGKAGGVFFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWK
IVEKYQVSRMFSAPTAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESGWPIMAIAR
GLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGCIQTIWGDDGRFVKTYWSLFSRPVYATFDWG
IRDADGYHFILGRTDDVINVAGHRLGTREIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDRDVAHSQEK
AIMALVDSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQAMEE
>Mature_627_residues
SFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFARWFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEE
RTFTFRQLHDEVNAVASMLRSLGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIVS
ADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFVSLRHQHIGARVPVAWLESNETSCILYTS
GTTGKPKGVQRDVGRYAVALATSMDTIFGGKAGGVFFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKI
VEKYQVSRMFSAPTAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESGWPIMAIARG
LDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGCIQTIWGDDGRFVKTYWSLFSRPVYATFDWGI
RDADGYHFILGRTDDVINVAGHRLGTREIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDRDVAHSQEKA
IMALVDSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQAMEE

Specific function: Catalyzes the synthesis of propionyl-CoA from propionate and CoA. Also converts acetate to acetyl-CoA but with a lower specific activity [H]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI13375727, Length=636, Percent_Identity=39.7798742138365, Blast_Score=457, Evalue=1e-128,
Organism=Homo sapiens, GI8923896, Length=656, Percent_Identity=35.2134146341463, Blast_Score=392, Evalue=1e-109,
Organism=Homo sapiens, GI28416953, Length=640, Percent_Identity=36.875, Blast_Score=390, Evalue=1e-108,
Organism=Homo sapiens, GI253970489, Length=669, Percent_Identity=34.678624813154, Blast_Score=389, Evalue=1e-108,
Organism=Homo sapiens, GI115511026, Length=557, Percent_Identity=27.1095152603232, Blast_Score=155, Evalue=9e-38,
Organism=Homo sapiens, GI42544132, Length=563, Percent_Identity=25.5772646536412, Blast_Score=155, Evalue=1e-37,
Organism=Homo sapiens, GI122937307, Length=606, Percent_Identity=26.5676567656766, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI58082049, Length=569, Percent_Identity=26.3620386643234, Blast_Score=150, Evalue=4e-36,
Organism=Homo sapiens, GI157311624, Length=568, Percent_Identity=26.4084507042254, Blast_Score=145, Evalue=1e-34,
Organism=Homo sapiens, GI157311622, Length=568, Percent_Identity=26.4084507042254, Blast_Score=145, Evalue=1e-34,
Organism=Homo sapiens, GI31982927, Length=641, Percent_Identity=23.4009360374415, Blast_Score=144, Evalue=3e-34,
Organism=Homo sapiens, GI38505220, Length=556, Percent_Identity=25.8992805755396, Blast_Score=143, Evalue=5e-34,
Organism=Homo sapiens, GI187761345, Length=413, Percent_Identity=24.2130750605327, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI187761343, Length=413, Percent_Identity=24.2130750605327, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI1786529, Length=628, Percent_Identity=99.3630573248408, Blast_Score=1287, Evalue=0.0,
Organism=Escherichia coli, GI1790505, Length=637, Percent_Identity=36.4207221350078, Blast_Score=412, Evalue=1e-116,
Organism=Escherichia coli, GI221142682, Length=544, Percent_Identity=24.4485294117647, Blast_Score=118, Evalue=1e-27,
Organism=Escherichia coli, GI1788107, Length=564, Percent_Identity=24.290780141844, Blast_Score=102, Evalue=9e-23,
Organism=Escherichia coli, GI1786810, Length=527, Percent_Identity=23.3396584440228, Blast_Score=97, Evalue=4e-21,
Organism=Escherichia coli, GI145693145, Length=404, Percent_Identity=24.009900990099, Blast_Score=86, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI71983001, Length=654, Percent_Identity=33.4862385321101, Blast_Score=350, Evalue=1e-96,
Organism=Caenorhabditis elegans, GI71982997, Length=654, Percent_Identity=33.4862385321101, Blast_Score=350, Evalue=1e-96,
Organism=Caenorhabditis elegans, GI17531443, Length=549, Percent_Identity=25.3187613843352, Blast_Score=119, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI17569507, Length=650, Percent_Identity=21.6923076923077, Blast_Score=119, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI17559526, Length=568, Percent_Identity=22.7112676056338, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI71985884, Length=582, Percent_Identity=21.9931271477663, Blast_Score=87, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6319264, Length=632, Percent_Identity=38.1329113924051, Blast_Score=418, Evalue=1e-117,
Organism=Saccharomyces cerevisiae, GI6323182, Length=644, Percent_Identity=37.111801242236, Blast_Score=416, Evalue=1e-117,
Organism=Saccharomyces cerevisiae, GI6319699, Length=386, Percent_Identity=22.7979274611399, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21355225, Length=641, Percent_Identity=38.5335413416537, Blast_Score=436, Evalue=1e-122,
Organism=Drosophila melanogaster, GI24667955, Length=650, Percent_Identity=34.7692307692308, Blast_Score=393, Evalue=1e-109,
Organism=Drosophila melanogaster, GI62472339, Length=562, Percent_Identity=36.1209964412811, Blast_Score=359, Evalue=4e-99,
Organism=Drosophila melanogaster, GI24667959, Length=529, Percent_Identity=35.7277882797732, Blast_Score=329, Evalue=4e-90,
Organism=Drosophila melanogaster, GI21355181, Length=529, Percent_Identity=23.8185255198488, Blast_Score=112, Evalue=6e-25,
Organism=Drosophila melanogaster, GI21358303, Length=511, Percent_Identity=23.8747553816047, Blast_Score=99, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24648260, Length=380, Percent_Identity=25.7894736842105, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI21356947, Length=375, Percent_Identity=24.5333333333333, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI19922652, Length=552, Percent_Identity=23.3695652173913, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21356441, Length=516, Percent_Identity=23.6434108527132, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI281365686, Length=519, Percent_Identity=22.7360308285164, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI20130357, Length=386, Percent_Identity=24.8704663212435, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24648253, Length=551, Percent_Identity=23.7749546279492, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24648255, Length=551, Percent_Identity=23.956442831216, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24648257, Length=372, Percent_Identity=26.6129032258064, Blast_Score=75, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR012694 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.17 [H]

Molecular weight: Translated: 69476; Mature: 69345

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFARWFCEGRTNLCHNAIDR
CCHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHCCCCCCHHHEEECCCCHHHHHHHHH
WLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRSLGVQRGDRVLVYMPMIAE
HHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHH
AHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIVSADAGARGGKIIPYKKLLDD
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCEEEEEECCCCCCCCCCCCHHHHHHH
AISQAQHQPRHVLLVDRGLAKMARVSGRDVDFVSLRHQHIGARVPVAWLESNETSCILYT
HHHHHHCCCCEEEEECCCHHHHHHHCCCCCHHHHHHHHHCCCCCEEEEEECCCCEEEEEE
SGTTGKPKGVQRDVGRYAVALATSMDTIFGGKAGGVFFCASDIGWVVGHSYIVYAPLLAG
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHCHHHHHHHHHHH
MATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAPTAIRVLKKFPTAEIRKHDLSSLEVLY
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHCCCCCEEEEE
LAGEPLDEPTASWVSNTLDVPVIDNYWQTESGWPIMAIARGLDDRPTRLGSPGVPMYGYN
EECCCCCCCHHHHHHCCCCCCEECCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCEECCC
VQLLNEVTGEPCGVNEKGMLVVEGPLPPGCIQTIWGDDGRFVKTYWSLFSRPVYATFDWG
HHHHHHCCCCCCCCCCCCEEEEECCCCCHHHHHHCCCCCCHHHHHHHHHCCCEEEEEECC
IRDADGYHFILGRTDDVINVAGHRLGTREIEESISSHPGVAEVAVVGVKDALKGQVAVAF
CCCCCCCEEEEECCCCHHHHHHCCCCHHHHHHHHHCCCCHHHEEEEEHHHHCCCCEEEEE
VIPKESDSLEDRDVAHSQEKAIMALVDSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQA
EEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHHHHHHH
ICEGRDPGDLTTIDDPASLDQIRQAMEE
HHCCCCCCCCCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure 
SFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFARWFCEGRTNLCHNAIDR
CHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHCCCCCCHHHEEECCCCHHHHHHHHH
WLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRSLGVQRGDRVLVYMPMIAE
HHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHH
AHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIVSADAGARGGKIIPYKKLLDD
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCEEEEEECCCCCCCCCCCCHHHHHHH
AISQAQHQPRHVLLVDRGLAKMARVSGRDVDFVSLRHQHIGARVPVAWLESNETSCILYT
HHHHHHCCCCEEEEECCCHHHHHHHCCCCCHHHHHHHHHCCCCCEEEEEECCCCEEEEEE
SGTTGKPKGVQRDVGRYAVALATSMDTIFGGKAGGVFFCASDIGWVVGHSYIVYAPLLAG
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHCHHHHHHHHHHH
MATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAPTAIRVLKKFPTAEIRKHDLSSLEVLY
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHCCCCCEEEEE
LAGEPLDEPTASWVSNTLDVPVIDNYWQTESGWPIMAIARGLDDRPTRLGSPGVPMYGYN
EECCCCCCCHHHHHHCCCCCCEECCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCEECCC
VQLLNEVTGEPCGVNEKGMLVVEGPLPPGCIQTIWGDDGRFVKTYWSLFSRPVYATFDWG
HHHHHHCCCCCCCCCCCCEEEEECCCCCHHHHHHCCCCCCHHHHHHHHHCCCEEEEEECC
IRDADGYHFILGRTDDVINVAGHRLGTREIEESISSHPGVAEVAVVGVKDALKGQVAVAF
CCCCCCCEEEEECCCCHHHHHHCCCCHHHHHHHHHCCCCHHHEEEEEHHHHCCCCEEEEE
VIPKESDSLEDRDVAHSQEKAIMALVDSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQA
EEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHHHHHHH
ICEGRDPGDLTTIDDPASLDQIRQAMEE
HHCCCCCCCCCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]