Click here to switch to the map view.

The map label for this gene is betT

Identifier: 157159826

GI number: 157159826

Start: 386803

End: 388836

Strand: Direct

Name: betT

Synonym: EcHS_A0373

Alternate gene names: 157159826

Gene position: 386803-388836 (Clockwise)

Preceding gene: 157159822

Following gene: 157159827

Centisome position: 8.33

GC content: 55.21

Gene sequence:

>2034_bases
ATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTT
GTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCT
TCGGTTGGTACTATCTACTGGCGGCAACGCTCTATATTGTCTTTGTGGTCTGTATCGCTTGTTCGCGTTTTGGTTCGGTG
AAGCTCGGGCCAGAACAATCCAAACCGGAATTCAGCCTGCTGAGTTGGGCGGCGATGCTGTTTGCTGCCGGGATCGGTAT
CGACCTGATGTTCTTCTCCGTAGCCGAACCGGTAACGCAGTATATGCAGCCGCCGGAAGGCGCGGGACAGACGATTGAGG
CCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATGTATGCGCTGATGGGCATGGCG
CTCGGATACTTTAGCTATCGTTATAATTTGCCGCTCACCATCCGCTCGGCGCTGTACCCGATCTTCGGTAAACGGATTAA
CGGGCCGATAGGGCACTCAGTGGATATTGCAGCGGTGATCGGCACCATCTTCGGTATTGCCACTACGCTCGGTATCGGTG
TGGTGCAGCTTAACTATGGCTTGAGCGTACTGTTTGATATTCCCGATTCGATGGCGGCAAAAGCGGCACTGATCGCCTTG
TCGGTGATAATCGCCACGATCTCTGTCACCTCCGGTGTCGATAAGGGGATTCGTGTGTTATCGGAACTTAACGTTGCGCT
GGCGCTGGGATTGATCCTGTTCGTATTGTTTATGGGCGACACCTCGTTCCTGCTTAATGCGCTGGTGCTGAATGTTGGCG
ACTATGTGAATCGCTTTATGGGCATGACGCTCAACAGTTTTGCCTTCGACCGTCCAGTTGAGTGGATGAATAACTGGACG
CTCTTCTTCTGGGCATGGTGGGTGGCATGGTCGCCGTTTGTCGGCTTGTTCCTGGCGCGTATCTCGCGTGGGCGTACCAT
TCGCCAGTTCGTGCTGGGCACGTTGATTATTCCGTTTACCTTCACGCTGTTATGGCTCTCGGTGTTCGGCAATAGCGCGC
TGTATGAAATCATCCACGGCGGCGCGGCATTTGCCGAGGAAGCGATGGTCCATCCGGAGCGCGGCTTCTACAGCCTGCTG
GCGCAGTATCCGGCGTTTACCTTTAGCGCCTCCGTCGCCACCATTACTGGCCTGCTGTTTTATGTGACCTCGGCGGACTC
CGGTGCGCTGGTGCTGGGGAATTTCACCTCGCAGCTTAAAGATATCAACAGCGACGCCCCCGGCTGGCTGCGCGTCTTCT
GGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACCAACGGGATATCCGCGCTGCAAAACACCACGGTGATT
ATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGTAGAAGATTACCGCCG
TGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGTCTCTCGCGCC
TGATGAATTATCCGGGCACGCGTTACACTAAACAGATGATGGAGACGGTCTGTTACCCGGCAATGGAAGAAGTGGCGCAG
GAGCTGCGGTTGCGCGGCGCATACGTGGAGCTAAAAAGCCTGCCGCCGGAAGAGGGACAACAGTTGGGGCATCTGGATTT
GTTGGTGCATATGGGCGAAGAACAAAACTTTGTCTATCAGATTTGGCCGCAGCAATATTCGGTGCCGGGCTTTACCTACC
GCGCACGTAGCGGTAAATCGACCTACTACCGGCTGGAAACCTTCCTGTTAGAAGGCAGCCAGGGCAACGACCTGATGGAC
TACAGCAAAGAGCAGGTGATCACCGATATTCTTGACCAGTACGAGCGGCACCTTAACTTTATTCATCTCCATCGTGAAGC
GCCGGGCCATAGCGTGATGTTCCCGGACGCGTGA

Upstream 100 bases:

>100_bases
TATCTATTAAAGCGGTTATTGATTGGACGTTCAATATAAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATGGTAAC
AATAACAGTGGGGATACTGG

Downstream 100 bases:

>100_bases
TTGTTTTTGCATAAAACCGCGCCATGTCTGCATATGGCGCGGGAATCATTGACTCGCTGTAAAAAATTATGCATATATTA
TATATGATTGTCATGTCATA

Product: choline transport protein BetT

Products: Proton [Cytoplasm]; choline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 677; Mature: 676

Protein sequence:

>677_residues
MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIGRTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSV
KLGPEQSKPEFSLLSWAAMLFAAGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLNYGLSVLFDIPDSMAAKAALIAL
SVIIATISVTSGVDKGIRVLSELNVALALGLILFVLFMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWT
LFFWAWWVAWSPFVGLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHPERGFYSLL
AQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWLRVFWSVAIGLLTLGMLMTNGISALQNTTVI
MGLPFSFVIFFVMAGLYKSLKVEDYRRESANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQ
ELRLRGAYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYRLETFLLEGSQGNDLMD
YSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA

Sequences:

>Translated_677_residues
MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIGRTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSV
KLGPEQSKPEFSLLSWAAMLFAAGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLNYGLSVLFDIPDSMAAKAALIAL
SVIIATISVTSGVDKGIRVLSELNVALALGLILFVLFMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWT
LFFWAWWVAWSPFVGLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHPERGFYSLL
AQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWLRVFWSVAIGLLTLGMLMTNGISALQNTTVI
MGLPFSFVIFFVMAGLYKSLKVEDYRRESANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQ
ELRLRGAYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYRLETFLLEGSQGNDLMD
YSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA
>Mature_676_residues
TDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIGRTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVK
LGPEQSKPEFSLLSWAAMLFAAGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMAL
GYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLNYGLSVLFDIPDSMAAKAALIALS
VIIATISVTSGVDKGIRVLSELNVALALGLILFVLFMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTL
FFWAWWVAWSPFVGLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHPERGFYSLLA
QYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWLRVFWSVAIGLLTLGMLMTNGISALQNTTVIM
GLPFSFVIFFVMAGLYKSLKVEDYRRESANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQE
LRLRGAYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYRLETFLLEGSQGNDLMDY
SKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA

Specific function: High-affinity uptake of choline driven by a proton- motive force

COG id: COG1292

COG function: function code M; Choline-glycine betaine transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCCT transporter (TC 2.A.15) family

Homologues:

Organism=Escherichia coli, GI1786506, Length=677, Percent_Identity=100, Blast_Score=1383, Evalue=0.0,
Organism=Escherichia coli, GI1788102, Length=452, Percent_Identity=28.0973451327434, Blast_Score=182, Evalue=9e-47,
Organism=Escherichia coli, GI1786224, Length=514, Percent_Identity=26.0700389105058, Blast_Score=151, Evalue=1e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BETT_ECO57 (P0ABD0)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   D85524
- PIR:   H90673
- RefSeq:   NP_286048.1
- RefSeq:   NP_308387.1
- ProteinModelPortal:   P0ABD0
- SMR:   P0ABD0
- EnsemblBacteria:   EBESCT00000024274
- EnsemblBacteria:   EBESCT00000057699
- GeneID:   914461
- GeneID:   957215
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z0401
- KEGG:   ecs:ECs0360
- GeneTree:   EBGT00050000009329
- HOGENOM:   HBG682943
- OMA:   TGWALYA
- ProtClustDB:   PRK09928
- BioCyc:   ECOL83334:ECS0360-MONOMER
- InterPro:   IPR000060
- InterPro:   IPR018093
- TIGRFAMs:   TIGR00842

Pfam domain/function: PF02028 BCCT

EC number: NA

Molecular weight: Translated: 75843; Mature: 75712

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: PS01303 BCCT

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x11fbe640)-; HASH(0x12c81b88)-; HASH(0x12316f1c)-; HASH(0x12989d88)-; HASH(0x12aca834)-; HASH(0x12c4e13c)-; HASH(0x124f7964)-; HASH(0x12659bb0)-; HASH(0x12c97514)-; HASH(0x11a42d08)-; HASH(0x12c68bf0)-; HASH(0x12a8d224)-;

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIGRTLDWVSKTFGWYYLL
CCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFAAGIGIDLMFFSVAEPVTQ
HHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
YMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMALGYFSYRYNLPLTIRSALYP
HCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHEEECCCEEHHHHHHH
IFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLNYGLSVLFDIPDSMAAKAALIAL
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEECCHHHHHHHHHHHH
SVIIATISVTSGVDKGIRVLSELNVALALGLILFVLFMGDTSFLLNALVLNVGDYVNRFM
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
GMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFVGLFLARISRGRTIRQFVLGTLIIPFT
CCHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
FTLLWLSVFGNSALYEIIHGGAAFAEEAMVHPERGFYSLLAQYPAFTFSASVATITGLLF
HHHHHHHHHCCHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHH
YVTSADSGALVLGNFTSQLKDINSDAPGWLRVFWSVAIGLLTLGMLMTNGISALQNTTVI
HEECCCCCEEEECCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
MGLPFSFVIFFVMAGLYKSLKVEDYRRESANRDTAPRPLGLQDRLSWKKRLSRLMNYPGT
EECCHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCC
RYTKQMMETVCYPAMEEVAQELRLRGAYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHCCCHHHEEECCCCCCEEEE
IWPQQYSVPGFTYRARSGKSTYYRLETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNF
ECCCCCCCCCCEEECCCCCCHHEEEEEHEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCE
IHLHREAPGHSVMFPDA
EEEEECCCCCCCCCCCH
>Mature Secondary Structure 
TDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIGRTLDWVSKTFGWYYLL
CCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFAAGIGIDLMFFSVAEPVTQ
HHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
YMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMALGYFSYRYNLPLTIRSALYP
HCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHEEECCCEEHHHHHHH
IFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLNYGLSVLFDIPDSMAAKAALIAL
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEECCHHHHHHHHHHHH
SVIIATISVTSGVDKGIRVLSELNVALALGLILFVLFMGDTSFLLNALVLNVGDYVNRFM
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
GMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFVGLFLARISRGRTIRQFVLGTLIIPFT
CCHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
FTLLWLSVFGNSALYEIIHGGAAFAEEAMVHPERGFYSLLAQYPAFTFSASVATITGLLF
HHHHHHHHHCCHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHH
YVTSADSGALVLGNFTSQLKDINSDAPGWLRVFWSVAIGLLTLGMLMTNGISALQNTTVI
HEECCCCCEEEECCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
MGLPFSFVIFFVMAGLYKSLKVEDYRRESANRDTAPRPLGLQDRLSWKKRLSRLMNYPGT
EECCHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCC
RYTKQMMETVCYPAMEEVAQELRLRGAYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHCCCHHHEEECCCCCCEEEE
IWPQQYSVPGFTYRARSGKSTYYRLETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNF
ECCCCCCCCCCEEECCCCCCHHEEEEEHEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCE
IHLHREAPGHSVMFPDA
EEEEECCCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; choline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + choline [Periplasm] = Proton [Cytoplasm] + choline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796