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The map label for this gene is prfH [H]

Identifier: 157159718

GI number: 157159718

Start: 283234

End: 283848

Strand: Direct

Name: prfH [H]

Synonym: EcHS_A0263

Alternate gene names: 157159718

Gene position: 283234-283848 (Clockwise)

Preceding gene: 157159717

Following gene: 157159720

Centisome position: 6.1

GC content: 53.5

Gene sequence:

>615_bases
ATGATCTTGCTACAACTCTCCTCAGCGCAGGGGCCGGAAGAATGTTGTCTCGCAGTGAAAAAGGCGCTGGAGCGATTAAT
TAAAGAAGCTGCCCGACAAGACGTCGCGGTAACGGTGTTGGAAACAGAAACGGGCCGCTACTCTGACACGCTGCGTTCAG
CTCTGGTTTCTCTGGATGGCGATAACGCATGGGCATTAAGCGAAAGCTGGTGCGGCACTATTCAGTGGATTTGTCCGAGT
CCGTATCGGCCTCATCATGGGCGCAAAAACTGGTTTCTGGGCATTGGGCGTTTTACCGCTGATGAGCAGGAACAATCGGA
TGCAATCCGTTATGAGACGTTGCGTTCGTCGGGGCCGGGCGGTCAACATGTCAATAAAACCGACTCGGCGGTACGCGCCA
CGCATTTGGCATCCGGTATTAGCGTGAAGGTTCAGTCAGAGCGTAGTCAGCATGCTAACAAGCGGCTGGCACGATTGCTG
ATTGCCTGGAAGCTGGAGCAGCAGCAACAGGAAAATAGCGCGGTGCTGAAATCGCAGCGGCGAATGTTCCATCACCAGAT
TGAACGTGGCAACCCGAGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAA

Upstream 100 bases:

>100_bases
CTGAAAGCGTGGTGCAATGTCTGGTGCAGGCCGGTTTAATTATTCCTGTCGCGCGGCTGCGTCCGGTGCTAACGCTCAAA
AACAGTGGAGGGAAAAAAGG

Downstream 100 bases:

>100_bases
TGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAG
CAGTTCAGTCAGCAGTGTCC

Product: peptide chain release factor-like protein

Products: NA

Alternate protein names: RF-H [H]

Number of amino acids: Translated: 204; Mature: 204

Protein sequence:

>204_residues
MILLQLSSAQGPEECCLAVKKALERLIKEAARQDVAVTVLETETGRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPS
PYRPHHGRKNWFLGIGRFTADEQEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG

Sequences:

>Translated_204_residues
MILLQLSSAQGPEECCLAVKKALERLIKEAARQDVAVTVLETETGRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPS
PYRPHHGRKNWFLGIGRFTADEQEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG
>Mature_204_residues
MILLQLSSAQGPEECCLAVKKALERLIKEAARQDVAVTVLETETGRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPS
PYRPHHGRKNWFLGIGRFTADEQEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG

Specific function: Could be involved in translational termination [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI34577120, Length=60, Percent_Identity=51.6666666666667, Blast_Score=65, Evalue=4e-11,
Organism=Escherichia coli, GI1787462, Length=95, Percent_Identity=45.2631578947368, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI2367172, Length=121, Percent_Identity=32.2314049586777, Blast_Score=65, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6321295, Length=57, Percent_Identity=57.8947368421053, Blast_Score=69, Evalue=5e-13,
Organism=Drosophila melanogaster, GI19921226, Length=64, Percent_Identity=54.6875, Blast_Score=68, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000352
- InterPro:   IPR017509 [H]

Pfam domain/function: PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 22939; Mature: 22939

Theoretical pI: Translated: 9.31; Mature: 9.31

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILLQLSSAQGPEECCLAVKKALERLIKEAARQDVAVTVLETETGRYSDTLRSALVSLDG
CEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHCCC
DNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADEQEQSDAIRYETLRSSGPG
CCCEEECCCCCCEEEEECCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCC
GQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLLIAWKLEQQQQENSAVLKSQR
CCCCCCCHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RMFHHQIERGNPRRTFTGMAFIEG
HHHHHHHHCCCCCCEEEEEEEECC
>Mature Secondary Structure
MILLQLSSAQGPEECCLAVKKALERLIKEAARQDVAVTVLETETGRYSDTLRSALVSLDG
CEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHCCC
DNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADEQEQSDAIRYETLRSSGPG
CCCEEECCCCCCEEEEECCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCC
GQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLLIAWKLEQQQQENSAVLKSQR
CCCCCCCHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RMFHHQIERGNPRRTFTGMAFIEG
HHHHHHHHCCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1695895; 9278503; 1408743 [H]