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The map label for this gene is cutF [H]

Identifier: 157159658

GI number: 157159658

Start: 213426

End: 214136

Strand: Direct

Name: cutF [H]

Synonym: EcHS_A0195

Alternate gene names: 157159658

Gene position: 213426-214136 (Clockwise)

Preceding gene: 157159657

Following gene: 157159659

Centisome position: 4.6

GC content: 51.62

Gene sequence:

>711_bases
ATGGTGAAAAAAGCGATAGTGACAGCGATGGCTGTAATCAGCCTCTTTACTCTGATGGGATGTAATAATCGGGCCGAAGT
TGATGCGCTTTCTCCGGCGCAGGCTGCCGAACTGAAACCGATGCCGCAAAGTTGGCGCGGCGTGCTGCCGTGTGCTGATT
GCGAAGGAATCGAAACCTCTCTGTTCCTCGAAAAAGACGGAACATGGGTGATGAATGAGCGTTATCTCGGTGCTCGTGAA
GAACCTTCCTCCTTCGCTTCCTACGGTACATGGGCGCGAACCGCTGACAAGCTGGTATTAACCGACAGCAAAGGTGAAAA
GTCATATTATCGGGCGAAAGGCGATGCGCTGGAGATGCTCGATCGTGAAGGCAATCCGATTGAATCGCAGTTCAACTATA
CGCTGGAACCGGCACAATCCAGTTTACCTATGACGCCGATGACCCTGCGAGGCATGTATTTTTATATGGCTGATGCGGCG
ACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCG
CGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGCTAATCCGGATACTGGTGCGCCAA
CCAAAGTATTAGCGCCCGATACGGCGGGTAAATTTTACCCGAATAAGGATTGCAGTAGTTTGGGGCAGTAA

Upstream 100 bases:

>100_bases
GCATCGAAAGAGCGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTACGCAGCGGTCG
GGAATAAAAAGAAGGAATAG

Downstream 100 bases:

>100_bases
ACCTGCTTAAGGCAGAAACAAACGCAAAATTGCCTGATGCGCTGCGCTTATCAGGCCTTGTATTATCCCTCCAGTGCAGA
GAAAATCGGCCAGTTTTCTC

Product: lipoprotein involved with copper homeostasis and adhesion

Products: NA

Alternate protein names: Copper homeostasis protein CutF [H]

Number of amino acids: Translated: 236; Mature: 236

Protein sequence:

>236_residues
MVKKAIVTAMAVISLFTLMGCNNRAEVDALSPAQAAELKPMPQSWRGVLPCADCEGIETSLFLEKDGTWVMNERYLGARE
EPSSFASYGTWARTADKLVLTDSKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLAPDTAGKFYPNKDCSSLGQ

Sequences:

>Translated_236_residues
MVKKAIVTAMAVISLFTLMGCNNRAEVDALSPAQAAELKPMPQSWRGVLPCADCEGIETSLFLEKDGTWVMNERYLGARE
EPSSFASYGTWARTADKLVLTDSKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLAPDTAGKFYPNKDCSSLGQ
>Mature_236_residues
MVKKAIVTAMAVISLFTLMGCNNRAEVDALSPAQAAELKPMPQSWRGVLPCADCEGIETSLFLEKDGTWVMNERYLGARE
EPSSFASYGTWARTADKLVLTDSKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLAPDTAGKFYPNKDCSSLGQ

Specific function: Involved in copper homeostasis. Could be involved in both copper efflux and the delivery of copper to copper-dependent enzymes. When overproduced induces degP through the activation of the two-component system CpxA/CpxR [H]

COG id: COG3015

COG function: function code MP; Uncharacterized lipoprotein NlpE involved in copper resistance

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786390, Length=236, Percent_Identity=98.3050847457627, Blast_Score=484, Evalue=1e-138,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007298 [H]

Pfam domain/function: PF04170 NlpE [H]

EC number: NA

Molecular weight: Translated: 25855; Mature: 25855

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00283 SOYBEAN_KUNITZ

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKKAIVTAMAVISLFTLMGCNNRAEVDALSPAQAAELKPMPQSWRGVLPCADCEGIETS
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCHHHCCCCCCCCCCCCCEE
LFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTDSKGEKSYYRAKGDALEML
EEEECCCCEEECCCCCCCCCCCHHHHHCCCHHHCCCEEEEECCCCCCHHHHCCCHHHHHH
DREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAATFTDCATGKRFMVANNAELE
HCCCCCCCCCCCEEECCCCCCCCCCCHHHHHEEEEEECCCHHHHHCCCCEEEEECCCHHH
RSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLAPDTAGKFYPNKDCSSLGQ
HHHHHHCCCCCCCEEEEECCEEEEECCCCCCCCCEEECCCCCCCCCCCCCHHHHCC
>Mature Secondary Structure
MVKKAIVTAMAVISLFTLMGCNNRAEVDALSPAQAAELKPMPQSWRGVLPCADCEGIETS
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCHHHCCCCCCCCCCCCCEE
LFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTDSKGEKSYYRAKGDALEML
EEEECCCCEEECCCCCCCCCCCHHHHHCCCHHHCCCEEEEECCCCCCHHHHCCCHHHHHH
DREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAATFTDCATGKRFMVANNAELE
HCCCCCCCCCCCEEECCCCCCCCCCCHHHHHEEEEEECCCHHHHHCCCCEEEEECCCHHH
RSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLAPDTAGKFYPNKDCSSLGQ
HHHHHHCCCCCCCEEEEECCEEEEECCCCCCCCCEEECCCCCCCCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7635808; 7635807; 9278503 [H]