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The map label for this gene is murF [H]

Identifier: 156980398

GI number: 156980398

Start: 2216009

End: 2217367

Strand: Reverse

Name: murF [H]

Synonym: SAHV_2067

Alternate gene names: 156980398

Gene position: 2217367-2216009 (Counterclockwise)

Preceding gene: 156980399

Following gene: 156980397

Centisome position: 76.99

GC content: 33.41

Gene sequence:

>1359_bases
ATGATTAATGTTACATTAAAGCAAATTCAATCATGGATTCCTTGTGAAATTGAAGATCAATTTTTAAATCAAGAGATAAA
TGGAGTCACAATTGATTCACGAGCAATTTCTAAAAATATGTTATTTATACCATTTAAAGGTGAAAATGTTGACGGTCATC
GCTTTGTCTCTAAAGCATTACAAGATGGTGCTGGGGCTGCTTTTTATCAAAGAGGGACACCTATAGATGAAAATGTAAGC
GGGCCTATTATATGGGTTGAAGACACATTAACGGCATTACAACAATTGGCACAAGCTTACTTGAGACATGTAAACCCTAA
AGTAATTGCCGTCACAGGGTCTAATGGTAAAACAACGACTAAAGATATGATTGAAAGTGTATTGCATACCGAATTTAAAG
TTAAGAAAACGCAAGGTAATTACAATAATGAAATTGGTTTACCTTTAACTATTTTGGAATTAGATAATGATACTGAAATA
TCAATATTGGAGATGGGGATGTCAGGTTTCCATGAAATTGAATTTCTGTCAAACCTCGCTCAACCAGATATTGCAGTTAT
AACTAATATTGGTGAGTCACATATGCAAGATTTAGGTTCGCGCGAGGGGATTGCTAAAGCTAAATCTGAAATTACAATAG
GTCTAAAAGATAATGGTACGTTTATATATGATGGCGATGAACCATTATTGAAACCACATGTTAAAGAAGTTGAAAATGCA
AAATGTATTAGTATTGGTGTTGCTACTGATAATGCATTAGTTTGTTCTGTTGATGATAGAGATACTACAGGTATTTCATT
TACGATTAATAATAAAGAACATTACGATCTGCCAATATTAGGAAAGCATAATATGAAAAATGCGACGATTGCCATTGCGG
TTGGTCATGAATTAGGTTTGACATATAACACAATCTATCAAAATTTAAAAAATGTCAGCTTAACTGGTATGCGTATGGAG
CAACATACATTAGAAAATGATATTACTGTGATAAATGATGCCTATAATGCAAGTCCTACAAGTATGAGAGCAGCTATTGA
TACACTGGGTACTTTGACAGGGCGTCGCATTCTAATTTTAGGAGATGTTTTAGAATTAGGTGAAAATAGCAAAGAAATGC
ATATCGGTGTAGGTAATTATTTAGAAGAAAAGCATATAGATGTGTTGTATACGTTTGGTAATGAAGCGAAGTATATTTAT
GATTCGGGCCAGCAACATGTCGAAAAAGCACAACACTTCAATTCTAAAGACGATATGATAGAAGTTTTAACAAGCGATTT
AAAAGCGCATGACCGTGTATTAGTTAAAGGATCACGTGGTATGAAGTTAGAAGAAGTTGTAAATGCTTTAATTTCATAG

Upstream 100 bases:

>100_bases
CTTATCCAGAATTGATTACAAAACTTATCGAGCTTGCTAAAGAACGTCACCAGGATAAACAGAAAAATAAATACAAAATT
GACTAACTGAGGTTGTTATT

Downstream 100 bases:

>100_bases
AGATTAGTCGAGCGACCTTTTACTTATAAAAATGATTTGAATTAATACTAAAAGATTACAAAGAAGAGGTGGTTTTGTGT
GTAAATACAAAATTGCCTTT

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate-D-alanyl-D-alanyl ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MINVTLKQIQSWIPCEIEDQFLNQEINGVTIDSRAISKNMLFIPFKGENVDGHRFVSKALQDGAGAAFYQRGTPIDENVS
GPIIWVEDTLTALQQLAQAYLRHVNPKVIAVTGSNGKTTTKDMIESVLHTEFKVKKTQGNYNNEIGLPLTILELDNDTEI
SILEMGMSGFHEIEFLSNLAQPDIAVITNIGESHMQDLGSREGIAKAKSEITIGLKDNGTFIYDGDEPLLKPHVKEVENA
KCISIGVATDNALVCSVDDRDTTGISFTINNKEHYDLPILGKHNMKNATIAIAVGHELGLTYNTIYQNLKNVSLTGMRME
QHTLENDITVINDAYNASPTSMRAAIDTLGTLTGRRILILGDVLELGENSKEMHIGVGNYLEEKHIDVLYTFGNEAKYIY
DSGQQHVEKAQHFNSKDDMIEVLTSDLKAHDRVLVKGSRGMKLEEVVNALIS

Sequences:

>Translated_452_residues
MINVTLKQIQSWIPCEIEDQFLNQEINGVTIDSRAISKNMLFIPFKGENVDGHRFVSKALQDGAGAAFYQRGTPIDENVS
GPIIWVEDTLTALQQLAQAYLRHVNPKVIAVTGSNGKTTTKDMIESVLHTEFKVKKTQGNYNNEIGLPLTILELDNDTEI
SILEMGMSGFHEIEFLSNLAQPDIAVITNIGESHMQDLGSREGIAKAKSEITIGLKDNGTFIYDGDEPLLKPHVKEVENA
KCISIGVATDNALVCSVDDRDTTGISFTINNKEHYDLPILGKHNMKNATIAIAVGHELGLTYNTIYQNLKNVSLTGMRME
QHTLENDITVINDAYNASPTSMRAAIDTLGTLTGRRILILGDVLELGENSKEMHIGVGNYLEEKHIDVLYTFGNEAKYIY
DSGQQHVEKAQHFNSKDDMIEVLTSDLKAHDRVLVKGSRGMKLEEVVNALIS
>Mature_452_residues
MINVTLKQIQSWIPCEIEDQFLNQEINGVTIDSRAISKNMLFIPFKGENVDGHRFVSKALQDGAGAAFYQRGTPIDENVS
GPIIWVEDTLTALQQLAQAYLRHVNPKVIAVTGSNGKTTTKDMIESVLHTEFKVKKTQGNYNNEIGLPLTILELDNDTEI
SILEMGMSGFHEIEFLSNLAQPDIAVITNIGESHMQDLGSREGIAKAKSEITIGLKDNGTFIYDGDEPLLKPHVKEVENA
KCISIGVATDNALVCSVDDRDTTGISFTINNKEHYDLPILGKHNMKNATIAIAVGHELGLTYNTIYQNLKNVSLTGMRME
QHTLENDITVINDAYNASPTSMRAAIDTLGTLTGRRILILGDVLELGENSKEMHIGVGNYLEEKHIDVLYTFGNEAKYIY
DSGQQHVEKAQHFNSKDDMIEVLTSDLKAHDRVLVKGSRGMKLEEVVNALIS

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=456, Percent_Identity=32.0175438596491, Blast_Score=203, Evalue=2e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 49984; Mature: 49984

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINVTLKQIQSWIPCEIEDQFLNQEINGVTIDSRAISKNMLFIPFKGENVDGHRFVSKAL
CCEEEHHHHHCCCCCCCCHHHHCCCCCCEEEECCCCCCCEEEEEECCCCCCHHHHHHHHH
QDGAGAAFYQRGTPIDENVSGPIIWVEDTLTALQQLAQAYLRHVNPKVIAVTGSNGKTTT
HCCCCCEEECCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCH
KDMIESVLHTEFKVKKTQGNYNNEIGLPLTILELDNDTEISILEMGMSGFHEIEFLSNLA
HHHHHHHHHCCEEEEECCCCCCCCCCCEEEEEEECCCCCEEEEECCCCCHHHHHHHHHCC
QPDIAVITNIGESHMQDLGSREGIAKAKSEITIGLKDNGTFIYDGDEPLLKPHVKEVENA
CCCEEEEECCCHHHHHHCCCCCCHHHCCCEEEEEEECCCEEEECCCCCCCCCHHHHCCCC
KCISIGVATDNALVCSVDDRDTTGISFTINNKEHYDLPILGKHNMKNATIAIAVGHELGL
EEEEEEEECCCEEEEEECCCCCCCEEEEECCCCCCCCCEECCCCCCCEEEEEEECCCCCC
TYNTIYQNLKNVSLTGMRMEQHTLENDITVINDAYNASPTSMRAAIDTLGTLTGRRILIL
CHHHHHHHHCCCEEEEEEHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEE
GDVLELGENSKEMHIGVGNYLEEKHIDVLYTFGNEAKYIYDSGQQHVEKAQHFNSKDDMI
ECHHHHCCCCCEEEEECCCHHCCCCCEEEEEECCCEEEEECCCHHHHHHHHHCCCHHHHH
EVLTSDLKAHDRVLVKGSRGMKLEEVVNALIS
HHHHHHHHCCCEEEEECCCCCCHHHHHHHHCC
>Mature Secondary Structure
MINVTLKQIQSWIPCEIEDQFLNQEINGVTIDSRAISKNMLFIPFKGENVDGHRFVSKAL
CCEEEHHHHHCCCCCCCCHHHHCCCCCCEEEECCCCCCCEEEEEECCCCCCHHHHHHHHH
QDGAGAAFYQRGTPIDENVSGPIIWVEDTLTALQQLAQAYLRHVNPKVIAVTGSNGKTTT
HCCCCCEEECCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCH
KDMIESVLHTEFKVKKTQGNYNNEIGLPLTILELDNDTEISILEMGMSGFHEIEFLSNLA
HHHHHHHHHCCEEEEECCCCCCCCCCCEEEEEEECCCCCEEEEECCCCCHHHHHHHHHCC
QPDIAVITNIGESHMQDLGSREGIAKAKSEITIGLKDNGTFIYDGDEPLLKPHVKEVENA
CCCEEEEECCCHHHHHHCCCCCCHHHCCCEEEEEEECCCEEEECCCCCCCCCHHHHCCCC
KCISIGVATDNALVCSVDDRDTTGISFTINNKEHYDLPILGKHNMKNATIAIAVGHELGL
EEEEEEEECCCEEEEEECCCCCCCEEEEECCCCCCCCCEECCCCCCCEEEEEEECCCCCC
TYNTIYQNLKNVSLTGMRMEQHTLENDITVINDAYNASPTSMRAAIDTLGTLTGRRILIL
CHHHHHHHHCCCEEEEEEHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEE
GDVLELGENSKEMHIGVGNYLEEKHIDVLYTFGNEAKYIYDSGQQHVEKAQHFNSKDDMI
ECHHHHCCCCCEEEEECCCHHCCCCCEEEEEECCCEEEEECCCHHHHHHHHHCCCHHHHH
EVLTSDLKAHDRVLVKGSRGMKLEEVVNALIS
HHHHHHHHCCCEEEEECCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]