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The map label for this gene is ilvB [H]

Identifier: 156937885

GI number: 156937885

Start: 978284

End: 980017

Strand: Direct

Name: ilvB [H]

Synonym: Igni_1097

Alternate gene names: 156937885

Gene position: 978284-980017 (Clockwise)

Preceding gene: 156937882

Following gene: 156937886

Centisome position: 75.4

GC content: 61.3

Gene sequence:

>1734_bases
GTGTACGTAGCTGACGCGCTGCTGGAGTCGTTAGAGAGGGAGGGCCTCAAGGTGGCCTTCGGAATACCGGGCAGCGCCAA
CCTGGGCCTTTACGAGAAGATACCGGACCACGACGTAGAAGTGATACTGATGAGGCACGAGCAAGGGGCCGCCCACGCCG
CCGACGCTTACGGAAGGGTCAAGAGGAAGGTCCCCGGCGTGGCCTTCGCCACCTCCGGGCCCGGTGCGACCAACCTCGTG
ACCGGCCTGGCCACCGCATACATGGACAGCGCCCCCATGATAGCCGTAACCGGGCAAGTCCCCACCGTGGTCATGGGGAG
GGACGCGTTCCAAGAGACGGACGTAGTGGGCGTAACGATGCCGATAACCAAGCACAGCTTCCTAGTTAGGAAGCCGGAGC
AGATAACTTGGGCCATAAGGGCCGCTAAGATGATATCCGTCACCAACAGGCCGGGCCCGGTGCTGGTGGACGTTCCCAGG
GACTTCTGGAGGGTGGACATTGACTTCTACTGGCCCAGCGACGAGGAGCTCCTAAGCGAGTGGATCCCGGGCTACCGGCC
CAACCCGCCCAAGCCCAACCCGGACGCGATAATGAAGGCTGCAAAGCTGCTGGTGCAAGCTGAGAACCCCGTAATACTGG
CCGGCGGGGGCGTTTGGTGGTCCTTCGCCACGGACGAGCTCCTCAAGCTCGCCGAGGTACTCATGGCGCCCGTGGTGACG
ACCCTCATGGGCAAGAACGCCGTCCCGGCGGATCACCCGCTGGTCTTGGGCAACGCCGGCATGCACGGCAGGCCAGAGGC
CAACTACGCATTATACAACGCCGACGTGGTCCTAGCCGTCGGGACGAGGTTCTCCGACAGAACGGTAGGCAACTTCGAAG
AGTTCAGGAGGGGGAAGAAGATAATACACATAGACATAGACAAGAGCGAGATAGGCAAGAACATCGAACCGGACGTGGGC
ATAGTGGCGGACGCCAGAGAGGCCTTGAGGGTCTTGAGGAACTACATCGTCGAGGCCCTAAACTTCAAGAGGAACGAGGA
GGTACCGCTCATCAGGAAGGCGAAGGAGTTCGGAGAGAGGGCTTGGGAGGTCCTCCTAAAGGAGAGGGGTAGGGGCTTGA
AGCCGTGGAAGGTCTTGAAGATAGCTAGGGAAGAGCTGCCGAGGGACGCGATAATAACCACCGGGGTAGGCGGGCACCAG
ATGTGGGCGGCCTTACATTACCCGGTCTACGTGCCGGGGACCTACTTGACCAGCGGGGGCCTGGGGACCATGGGCTACGG
CTTCCCGGCGGCCATAGGGGCTAAGGCGGCCGCCCCGGACAAGCCGGTGTTGGACATAGACGGCGACGGCAGCTTCCTCA
TGACCTCCCAGAACTTGGCCACCGTAAAGCAGTACAACCTCAACGTGGGGGTGCTGGTGTTCGACAACGGCTCCCTCCAG
CTGATAAGGCAGTGGCAAGACCTCTTCTTCAGCCACAGGAGGGTGGGCGAGAACCTAGCGTACTTGCCGGACTTCGTGAA
GCTTGCGGAGAGCTTCGGGATAGAGGGGGTGAGGCCGGAGACCTACGAAGAGATGAGGAAGGCTCTGAGGAGGGTGGCCC
AGGGGGAGGCGCTGGTGATAGACTACATCGTCGACCCGGACATAATGGTGCTGCCAATGGTAGCGCCCGGCAAGGTGATA
TCGCCCGAGAACCTAATCTTAACCCCCGAACAAGCGGCCGGGATAAAGGTGTGA

Upstream 100 bases:

>100_bases
GATGCCTTGGAATTGAGTTAACTTTCCCCGGACCCGTCTCGGGAAGGAGAGGAGCCGAGGGCAGCTCCTCAACAACAAAG
GAGAGGAGGGGTGGTGCGAG

Downstream 100 bases:

>100_bases
GGAGGTGGTAGCGATGAGCGAGGTGGAGACCTACGTCATAAGGTTCTCCATATACGCAGCGGTGGACGGCGGGCTGGACG
GCCTGGCGAGGGCGATACAC

Product: acetolactate synthase, large subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 577; Mature: 577

Protein sequence:

>577_residues
MYVADALLESLEREGLKVAFGIPGSANLGLYEKIPDHDVEVILMRHEQGAAHAADAYGRVKRKVPGVAFATSGPGATNLV
TGLATAYMDSAPMIAVTGQVPTVVMGRDAFQETDVVGVTMPITKHSFLVRKPEQITWAIRAAKMISVTNRPGPVLVDVPR
DFWRVDIDFYWPSDEELLSEWIPGYRPNPPKPNPDAIMKAAKLLVQAENPVILAGGGVWWSFATDELLKLAEVLMAPVVT
TLMGKNAVPADHPLVLGNAGMHGRPEANYALYNADVVLAVGTRFSDRTVGNFEEFRRGKKIIHIDIDKSEIGKNIEPDVG
IVADAREALRVLRNYIVEALNFKRNEEVPLIRKAKEFGERAWEVLLKERGRGLKPWKVLKIAREELPRDAIITTGVGGHQ
MWAALHYPVYVPGTYLTSGGLGTMGYGFPAAIGAKAAAPDKPVLDIDGDGSFLMTSQNLATVKQYNLNVGVLVFDNGSLQ
LIRQWQDLFFSHRRVGENLAYLPDFVKLAESFGIEGVRPETYEEMRKALRRVAQGEALVIDYIVDPDIMVLPMVAPGKVI
SPENLILTPEQAAGIKV

Sequences:

>Translated_577_residues
MYVADALLESLEREGLKVAFGIPGSANLGLYEKIPDHDVEVILMRHEQGAAHAADAYGRVKRKVPGVAFATSGPGATNLV
TGLATAYMDSAPMIAVTGQVPTVVMGRDAFQETDVVGVTMPITKHSFLVRKPEQITWAIRAAKMISVTNRPGPVLVDVPR
DFWRVDIDFYWPSDEELLSEWIPGYRPNPPKPNPDAIMKAAKLLVQAENPVILAGGGVWWSFATDELLKLAEVLMAPVVT
TLMGKNAVPADHPLVLGNAGMHGRPEANYALYNADVVLAVGTRFSDRTVGNFEEFRRGKKIIHIDIDKSEIGKNIEPDVG
IVADAREALRVLRNYIVEALNFKRNEEVPLIRKAKEFGERAWEVLLKERGRGLKPWKVLKIAREELPRDAIITTGVGGHQ
MWAALHYPVYVPGTYLTSGGLGTMGYGFPAAIGAKAAAPDKPVLDIDGDGSFLMTSQNLATVKQYNLNVGVLVFDNGSLQ
LIRQWQDLFFSHRRVGENLAYLPDFVKLAESFGIEGVRPETYEEMRKALRRVAQGEALVIDYIVDPDIMVLPMVAPGKVI
SPENLILTPEQAAGIKV
>Mature_577_residues
MYVADALLESLEREGLKVAFGIPGSANLGLYEKIPDHDVEVILMRHEQGAAHAADAYGRVKRKVPGVAFATSGPGATNLV
TGLATAYMDSAPMIAVTGQVPTVVMGRDAFQETDVVGVTMPITKHSFLVRKPEQITWAIRAAKMISVTNRPGPVLVDVPR
DFWRVDIDFYWPSDEELLSEWIPGYRPNPPKPNPDAIMKAAKLLVQAENPVILAGGGVWWSFATDELLKLAEVLMAPVVT
TLMGKNAVPADHPLVLGNAGMHGRPEANYALYNADVVLAVGTRFSDRTVGNFEEFRRGKKIIHIDIDKSEIGKNIEPDVG
IVADAREALRVLRNYIVEALNFKRNEEVPLIRKAKEFGERAWEVLLKERGRGLKPWKVLKIAREELPRDAIITTGVGGHQ
MWAALHYPVYVPGTYLTSGGLGTMGYGFPAAIGAKAAAPDKPVLDIDGDGSFLMTSQNLATVKQYNLNVGVLVFDNGSLQ
LIRQWQDLFFSHRRVGENLAYLPDFVKLAESFGIEGVRPETYEEMRKALRRVAQGEALVIDYIVDPDIMVLPMVAPGKVI
SPENLILTPEQAAGIKV

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=548, Percent_Identity=25.9124087591241, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI21361361, Length=581, Percent_Identity=26.3339070567986, Blast_Score=137, Evalue=2e-32,
Organism=Escherichia coli, GI87081685, Length=559, Percent_Identity=38.9982110912343, Blast_Score=385, Evalue=1e-108,
Organism=Escherichia coli, GI1790104, Length=557, Percent_Identity=38.0610412926391, Blast_Score=342, Evalue=3e-95,
Organism=Escherichia coli, GI1786717, Length=566, Percent_Identity=33.0388692579505, Blast_Score=290, Evalue=2e-79,
Organism=Escherichia coli, GI1787096, Length=586, Percent_Identity=27.9863481228669, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI1788716, Length=551, Percent_Identity=26.3157894736842, Blast_Score=146, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI17542570, Length=541, Percent_Identity=27.5415896487985, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17531299, Length=556, Percent_Identity=25.8992805755396, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17531301, Length=571, Percent_Identity=25.7443082311734, Blast_Score=127, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6323755, Length=566, Percent_Identity=38.86925795053, Blast_Score=350, Evalue=4e-97,
Organism=Saccharomyces cerevisiae, GI6320816, Length=552, Percent_Identity=24.6376811594203, Blast_Score=110, Evalue=8e-25,
Organism=Saccharomyces cerevisiae, GI6323163, Length=528, Percent_Identity=24.2424242424242, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6323073, Length=507, Percent_Identity=26.4299802761341, Blast_Score=97, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6321524, Length=535, Percent_Identity=23.7383177570093, Blast_Score=90, Evalue=8e-19,
Organism=Drosophila melanogaster, GI19922626, Length=549, Percent_Identity=27.3224043715847, Blast_Score=153, Evalue=3e-37,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 63298; Mature: 63298

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYVADALLESLEREGLKVAFGIPGSANLGLYEKIPDHDVEVILMRHEQGAAHAADAYGRV
CCHHHHHHHHHHHCCCEEEEECCCCCCCCHHCCCCCCCEEEEEEECCCCCCHHHHHHHHH
KRKVPGVAFATSGPGATNLVTGLATAYMDSAPMIAVTGQVPTVVMGRDAFQETDVVGVTM
HHCCCCEEEECCCCCHHHHHHHHHHHHHCCCCEEEEECCCCEEEECCCCCCCCCEEEEEE
PITKHSFLVRKPEQITWAIRAAKMISVTNRPGPVLVDVPRDFWRVDIDFYWPSDEELLSE
CCCCCCEEEECCHHHHHHHHHEEEEEECCCCCCEEEECCCCCEEEEEEEECCCHHHHHHH
WIPGYRPNPPKPNPDAIMKAAKLLVQAENPVILAGGGVWWSFATDELLKLAEVLMAPVVT
HCCCCCCCCCCCCHHHHHHHHHHHEECCCCEEEECCCEEEECCHHHHHHHHHHHHHHHHH
TLMGKNAVPADHPLVLGNAGMHGRPEANYALYNADVVLAVGTRFSDRTVGNFEEFRRGKK
HHHCCCCCCCCCCEEEECCCCCCCCCCCEEEEECCEEEEECCCCCCCCCCCHHHHHCCCE
IIHIDIDKSEIGKNIEPDVGIVADAREALRVLRNYIVEALNFKRNEEVPLIRKAKEFGER
EEEEECCHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
AWEVLLKERGRGLKPWKVLKIAREELPRDAIITTGVGGHQMWAALHYPVYVPGTYLTSGG
HHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEECCCCCHHHHHEEECCEEECCCEEECCC
LGTMGYGFPAAIGAKAAAPDKPVLDIDGDGSFLMTSQNLATVKQYNLNVGVLVFDNGSLQ
CCCCCCCCHHHHCCCCCCCCCCEEEECCCCCEEEECCCCHHHEEECCEEEEEEECCCCHH
LIRQWQDLFFSHRRVGENLAYLPDFVKLAESFGIEGVRPETYEEMRKALRRVAQGEALVI
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCEEEE
DYIVDPDIMVLPMVAPGKVISPENLILTPEQAAGIKV
EEEECCCEEEEEEECCCCEECCCCEEECCCHHCCCCC
>Mature Secondary Structure
MYVADALLESLEREGLKVAFGIPGSANLGLYEKIPDHDVEVILMRHEQGAAHAADAYGRV
CCHHHHHHHHHHHCCCEEEEECCCCCCCCHHCCCCCCCEEEEEEECCCCCCHHHHHHHHH
KRKVPGVAFATSGPGATNLVTGLATAYMDSAPMIAVTGQVPTVVMGRDAFQETDVVGVTM
HHCCCCEEEECCCCCHHHHHHHHHHHHHCCCCEEEEECCCCEEEECCCCCCCCCEEEEEE
PITKHSFLVRKPEQITWAIRAAKMISVTNRPGPVLVDVPRDFWRVDIDFYWPSDEELLSE
CCCCCCEEEECCHHHHHHHHHEEEEEECCCCCCEEEECCCCCEEEEEEEECCCHHHHHHH
WIPGYRPNPPKPNPDAIMKAAKLLVQAENPVILAGGGVWWSFATDELLKLAEVLMAPVVT
HCCCCCCCCCCCCHHHHHHHHHHHEECCCCEEEECCCEEEECCHHHHHHHHHHHHHHHHH
TLMGKNAVPADHPLVLGNAGMHGRPEANYALYNADVVLAVGTRFSDRTVGNFEEFRRGKK
HHHCCCCCCCCCCEEEECCCCCCCCCCCEEEEECCEEEEECCCCCCCCCCCHHHHHCCCE
IIHIDIDKSEIGKNIEPDVGIVADAREALRVLRNYIVEALNFKRNEEVPLIRKAKEFGER
EEEEECCHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
AWEVLLKERGRGLKPWKVLKIAREELPRDAIITTGVGGHQMWAALHYPVYVPGTYLTSGG
HHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEECCCCCHHHHHEEECCEEECCCEEECCC
LGTMGYGFPAAIGAKAAAPDKPVLDIDGDGSFLMTSQNLATVKQYNLNVGVLVFDNGSLQ
CCCCCCCCHHHHCCCCCCCCCCEEEECCCCCEEEECCCCHHHEEECCEEEEEEECCCCHH
LIRQWQDLFFSHRRVGENLAYLPDFVKLAESFGIEGVRPETYEEMRKALRRVAQGEALVI
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCEEEE
DYIVDPDIMVLPMVAPGKVISPENLILTPEQAAGIKV
EEEECCCEEEEEEECCCCEECCCCEEECCCHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]