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The map label for this gene is menE [H]

Identifier: 156937173

GI number: 156937173

Start: 333742

End: 335166

Strand: Reverse

Name: menE [H]

Synonym: Igni_0379

Alternate gene names: 156937173

Gene position: 335166-333742 (Counterclockwise)

Preceding gene: 156937174

Following gene: 156937168

Centisome position: 25.83

GC content: 58.74

Gene sequence:

>1425_bases
GTGTACCACGACCCCGGCAAGCCCGTCCATAAGATAATAGAAGAGAGGGCGCGGGAGAGCCCGGACAAGGTAGCTCTGGT
ATACGAGGACGGGACCGAACTGACCTACAAAGACTTGATATCGAAGGCTAAGGCCGTCGCTAACTTGCTGTACGAAAACA
AGGTAAGGAAGTGCGACACCGTCTTCCTAATAATGTTCAACAGGCCGGAGTTCGTCACCTCGCTGTTGGGGGCCCTCTAC
GCGGGGGCGAGGGTCGTCATAGTGGACGCCTTGACCCAGAAGGAAGACCTAGCCTTCCAGCTCAACGACAGCGCCCCGAA
GGTCGTGCTGGCGGACGAGGAGGTCCTCCAGAGGGAGAGCGAGACCTTGAAGGGCTACGCGGTCCTCACAGAGAAGGACC
TCTCCTCCGCCTCCGGGGAGCACGAGGTCGTCGTGGGCTACGAGGACGACGCGAGGGTCTTCTACTACGCCGGCATCGCG
GGGAGGACCATGCAAGTGATCCACTCCCACCGCTCCTTCACCGGGGCTATAATGCCCCTCGTACAGGCTGAAGGGATAAA
TGCTAACGACGTGAGCTTGGTTACGGTTCCCCTAACCCACGTGTTGGGCTTGGACGCGGCCCTCCTCAGCGCGCTGGTCT
CCGGAGCCACTGCCTTGTTGCTCAAGAAGTTCAACATGGATAAAATAAAGGAAATGGTAGCCAGGAGGAGCCCCACCTAC
TTGGTGGCGGTCCCCTTGGTGTTCCAAACCCTCATGAAGGAGGACGAGGAGTTCGTCAAGTCCCTAGGTCAGAGCTTGAG
GTGGGCGATGAGCGGCGGCGCCTACTTGCCTCCCGACGACCAGAGGAGGTGGGAGGAGCTCACCGGCAAGCCGCTGCTGC
AAGTCTACGGCATGACTGAGGCCCCTCAGATATTCGCGACCACCCCGGAGAAGCACAAGATCGGCAGCTTGGGCTTCCCC
TTGCCCGGCGTGGAGGCCTTGCTGGTGAACCCGGAGACCCTAGAGCCCGTGGAGGACCAAGGGGAGCTCCTAGTGAGAGG
TCCCCAAGTGATGAAGTGTTACCCCGACCCGGAGGAGAACTCCAAGGCCTTCGTTGAGGTTAACGGAAAGGTCTGGATGA
GGACGGGCGACATACTCGCCAAGGACGAGGAGGGCTTCTATTACTTCAGAGGGGTAAGGAAGAGGATGTTGAAGTACAAG
GGTTATCCGATATTCCCCAAGGACTTAGAGCTGATACTGCTCAAGCACCCGTGCGTAGCCGAAGCGGAGGTCGTGGGCGA
GCCGGCGGGCGAAGTGGGCCAGATACCCGTAGCGAAGGTAAAGCTCAAGGAGGGCTGCAAGGCCACCCCGGAGGAAATAA
TGGACTTCGTGAACAAGAGGGTAGCGGCCTACAAAAAGGTGAGGAAAGTGATCATTTTGGAATAA

Upstream 100 bases:

>100_bases
CGGACGAGTCCTTGTTGGAGGGCGCGGAGGAGGAGGGAGAAGAGGAGTGGGAGCCGGAGTAAATATTTCCTTCCTTCTCT
AGCCGAGGGCGGTGGCCCCG

Downstream 100 bases:

>100_bases
CCCTAAAGAAGAGCGCGTACTCTATCAAGCCCGTCTCCCCGTCGGCTCTAACCTTTCTGAGCACCGCTTGGACCTCCGAG
CCCTCCTTCGGCTCTTCCAT

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: OSB-CoA synthetase; o-succinylbenzoyl-CoA synthetase [H]

Number of amino acids: Translated: 474; Mature: 474

Protein sequence:

>474_residues
MYHDPGKPVHKIIEERARESPDKVALVYEDGTELTYKDLISKAKAVANLLYENKVRKCDTVFLIMFNRPEFVTSLLGALY
AGARVVIVDALTQKEDLAFQLNDSAPKVVLADEEVLQRESETLKGYAVLTEKDLSSASGEHEVVVGYEDDARVFYYAGIA
GRTMQVIHSHRSFTGAIMPLVQAEGINANDVSLVTVPLTHVLGLDAALLSALVSGATALLLKKFNMDKIKEMVARRSPTY
LVAVPLVFQTLMKEDEEFVKSLGQSLRWAMSGGAYLPPDDQRRWEELTGKPLLQVYGMTEAPQIFATTPEKHKIGSLGFP
LPGVEALLVNPETLEPVEDQGELLVRGPQVMKCYPDPEENSKAFVEVNGKVWMRTGDILAKDEEGFYYFRGVRKRMLKYK
GYPIFPKDLELILLKHPCVAEAEVVGEPAGEVGQIPVAKVKLKEGCKATPEEIMDFVNKRVAAYKKVRKVIILE

Sequences:

>Translated_474_residues
MYHDPGKPVHKIIEERARESPDKVALVYEDGTELTYKDLISKAKAVANLLYENKVRKCDTVFLIMFNRPEFVTSLLGALY
AGARVVIVDALTQKEDLAFQLNDSAPKVVLADEEVLQRESETLKGYAVLTEKDLSSASGEHEVVVGYEDDARVFYYAGIA
GRTMQVIHSHRSFTGAIMPLVQAEGINANDVSLVTVPLTHVLGLDAALLSALVSGATALLLKKFNMDKIKEMVARRSPTY
LVAVPLVFQTLMKEDEEFVKSLGQSLRWAMSGGAYLPPDDQRRWEELTGKPLLQVYGMTEAPQIFATTPEKHKIGSLGFP
LPGVEALLVNPETLEPVEDQGELLVRGPQVMKCYPDPEENSKAFVEVNGKVWMRTGDILAKDEEGFYYFRGVRKRMLKYK
GYPIFPKDLELILLKHPCVAEAEVVGEPAGEVGQIPVAKVKLKEGCKATPEEIMDFVNKRVAAYKKVRKVIILE
>Mature_474_residues
MYHDPGKPVHKIIEERARESPDKVALVYEDGTELTYKDLISKAKAVANLLYENKVRKCDTVFLIMFNRPEFVTSLLGALY
AGARVVIVDALTQKEDLAFQLNDSAPKVVLADEEVLQRESETLKGYAVLTEKDLSSASGEHEVVVGYEDDARVFYYAGIA
GRTMQVIHSHRSFTGAIMPLVQAEGINANDVSLVTVPLTHVLGLDAALLSALVSGATALLLKKFNMDKIKEMVARRSPTY
LVAVPLVFQTLMKEDEEFVKSLGQSLRWAMSGGAYLPPDDQRRWEELTGKPLLQVYGMTEAPQIFATTPEKHKIGSLGFP
LPGVEALLVNPETLEPVEDQGELLVRGPQVMKCYPDPEENSKAFVEVNGKVWMRTGDILAKDEEGFYYFRGVRKRMLKYK
GYPIFPKDLELILLKHPCVAEAEVVGEPAGEVGQIPVAKVKLKEGCKATPEEIMDFVNKRVAAYKKVRKVIILE

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Homo sapiens, GI187761345, Length=515, Percent_Identity=28.7378640776699, Blast_Score=158, Evalue=1e-38,
Organism=Homo sapiens, GI187761343, Length=515, Percent_Identity=28.7378640776699, Blast_Score=158, Evalue=1e-38,
Organism=Homo sapiens, GI156151445, Length=286, Percent_Identity=31.4685314685315, Blast_Score=114, Evalue=3e-25,
Organism=Homo sapiens, GI38505220, Length=500, Percent_Identity=24.8, Blast_Score=112, Evalue=7e-25,
Organism=Homo sapiens, GI42544132, Length=502, Percent_Identity=24.9003984063745, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI157311624, Length=472, Percent_Identity=24.1525423728814, Blast_Score=109, Evalue=7e-24,
Organism=Homo sapiens, GI157311622, Length=472, Percent_Identity=24.1525423728814, Blast_Score=109, Evalue=7e-24,
Organism=Homo sapiens, GI58082049, Length=481, Percent_Identity=23.2848232848233, Blast_Score=102, Evalue=7e-22,
Organism=Homo sapiens, GI115511026, Length=493, Percent_Identity=24.3407707910751, Blast_Score=102, Evalue=9e-22,
Organism=Homo sapiens, GI122937307, Length=497, Percent_Identity=24.5472837022133, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI8923896, Length=518, Percent_Identity=23.9382239382239, Blast_Score=83, Evalue=5e-16,
Organism=Homo sapiens, GI253970489, Length=327, Percent_Identity=25.3822629969419, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1788107, Length=510, Percent_Identity=27.6470588235294, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI145693145, Length=493, Percent_Identity=25.3549695740365, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI221142682, Length=497, Percent_Identity=25.7545271629779, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1789201, Length=267, Percent_Identity=28.4644194756554, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1790505, Length=512, Percent_Identity=23.828125, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI1786810, Length=473, Percent_Identity=23.2558139534884, Blast_Score=75, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17560140, Length=499, Percent_Identity=28.4569138276553, Blast_Score=157, Evalue=8e-39,
Organism=Caenorhabditis elegans, GI17558820, Length=487, Percent_Identity=27.7207392197125, Blast_Score=145, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17531443, Length=513, Percent_Identity=26.9005847953216, Blast_Score=135, Evalue=7e-32,
Organism=Caenorhabditis elegans, GI32563687, Length=479, Percent_Identity=26.9311064718163, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17557194, Length=519, Percent_Identity=26.5895953757225, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17559526, Length=508, Percent_Identity=25.9842519685039, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI71994703, Length=508, Percent_Identity=23.4251968503937, Blast_Score=112, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI71994694, Length=508, Percent_Identity=23.4251968503937, Blast_Score=112, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI71994690, Length=508, Percent_Identity=23.4251968503937, Blast_Score=111, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI17538037, Length=513, Percent_Identity=24.9512670565302, Blast_Score=105, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI71996755, Length=460, Percent_Identity=23.9130434782609, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71985884, Length=326, Percent_Identity=27.3006134969325, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17551278, Length=501, Percent_Identity=23.3532934131737, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17560308, Length=288, Percent_Identity=27.4305555555556, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI25147511, Length=179, Percent_Identity=35.195530726257, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6319699, Length=505, Percent_Identity=25.7425742574257, Blast_Score=122, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6323182, Length=499, Percent_Identity=23.8476953907816, Blast_Score=90, Evalue=8e-19,
Organism=Drosophila melanogaster, GI18859661, Length=527, Percent_Identity=27.7039848197343, Blast_Score=148, Evalue=9e-36,
Organism=Drosophila melanogaster, GI24581924, Length=496, Percent_Identity=25.6048387096774, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI21355181, Length=512, Percent_Identity=23.6328125, Blast_Score=115, Evalue=7e-26,
Organism=Drosophila melanogaster, GI24656500, Length=457, Percent_Identity=24.72647702407, Blast_Score=99, Evalue=9e-21,
Organism=Drosophila melanogaster, GI21356947, Length=491, Percent_Identity=23.0142566191446, Blast_Score=95, Evalue=9e-20,
Organism=Drosophila melanogaster, GI21356441, Length=498, Percent_Identity=22.8915662650602, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI19922652, Length=509, Percent_Identity=21.8074656188605, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24648253, Length=485, Percent_Identity=22.2680412371134, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24648255, Length=485, Percent_Identity=22.4742268041237, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24648260, Length=489, Percent_Identity=22.2903885480573, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24653035, Length=478, Percent_Identity=21.1297071129707, Blast_Score=69, Evalue=9e-12,
Organism=Drosophila melanogaster, GI20130357, Length=501, Percent_Identity=22.5548902195609, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192
- InterPro:   IPR023015 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 52730; Mature: 52730

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYHDPGKPVHKIIEERARESPDKVALVYEDGTELTYKDLISKAKAVANLLYENKVRKCDT
CCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCE
VFLIMFNRPEFVTSLLGALYAGARVVIVDALTQKEDLAFQLNDSAPKVVLADEEVLQRES
EEEEEECCHHHHHHHHHHHHCCCEEEEEEECCCCCCCEEEECCCCCEEEECCHHHHHHHH
ETLKGYAVLTEKDLSSASGEHEVVVGYEDDARVFYYAGIAGRTMQVIHSHRSFTGAIMPL
HHHCEEEEEEECCCCCCCCCCEEEEEECCCCEEEEEECCCCHHHHHHHHHCCHHHHHHHH
VQAEGINANDVSLVTVPLTHVLGLDAALLSALVSGATALLLKKFNMDKIKEMVARRSPTY
HCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCE
LVAVPLVFQTLMKEDEEFVKSLGQSLRWAMSGGAYLPPDDQRRWEELTGKPLLQVYGMTE
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCEEEEECCCC
APQIFATTPEKHKIGSLGFPLPGVEALLVNPETLEPVEDQGELLVRGPQVMKCYPDPEEN
CCEEEECCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCEEEECCCEEEECCCCCCC
SKAFVEVNGKVWMRTGDILAKDEEGFYYFRGVRKRMLKYKGYPIFPKDLELILLKHPCVA
CEEEEEECCEEEEEECCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCC
EAEVVGEPAGEVGQIPVAKVKLKEGCKATPEEIMDFVNKRVAAYKKVRKVIILE
CHHHCCCCCCCCCCCCCEEEEHHCCCCCCHHHHHHHHHHHHHHHHHHHHHEECC
>Mature Secondary Structure
MYHDPGKPVHKIIEERARESPDKVALVYEDGTELTYKDLISKAKAVANLLYENKVRKCDT
CCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCE
VFLIMFNRPEFVTSLLGALYAGARVVIVDALTQKEDLAFQLNDSAPKVVLADEEVLQRES
EEEEEECCHHHHHHHHHHHHCCCEEEEEEECCCCCCCEEEECCCCCEEEECCHHHHHHHH
ETLKGYAVLTEKDLSSASGEHEVVVGYEDDARVFYYAGIAGRTMQVIHSHRSFTGAIMPL
HHHCEEEEEEECCCCCCCCCCEEEEEECCCCEEEEEECCCCHHHHHHHHHCCHHHHHHHH
VQAEGINANDVSLVTVPLTHVLGLDAALLSALVSGATALLLKKFNMDKIKEMVARRSPTY
HCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCE
LVAVPLVFQTLMKEDEEFVKSLGQSLRWAMSGGAYLPPDDQRRWEELTGKPLLQVYGMTE
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCEEEEECCCC
APQIFATTPEKHKIGSLGFPLPGVEALLVNPETLEPVEDQGELLVRGPQVMKCYPDPEEN
CCEEEECCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCEEEECCCEEEECCCCCCC
SKAFVEVNGKVWMRTGDILAKDEEGFYYFRGVRKRMLKYKGYPIFPKDLELILLKHPCVA
CEEEEEECCEEEEEECCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCC
EAEVVGEPAGEVGQIPVAKVKLKEGCKATPEEIMDFVNKRVAAYKKVRKVIILE
CHHHCCCCCCCCCCCCCEEEEHHCCCCCCHHHHHHHHHHHHHHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1629163; 8566759; 9387221; 9384377 [H]