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The map label for this gene is thrS [H]

Identifier: 156937107

GI number: 156937107

Start: 266591

End: 268249

Strand: Reverse

Name: thrS [H]

Synonym: Igni_0313

Alternate gene names: 156937107

Gene position: 268249-266591 (Counterclockwise)

Preceding gene: 156937108

Following gene: 156937106

Centisome position: 20.67

GC content: 56.9

Gene sequence:

>1659_bases
ATGAAAGTGTTGATAGTGCGCAGCAAGAGCTTCGCGGCCGGGAACGAGAGGCTGGACGACGCTTTGGTCGCCTTCGTCGC
CGTGGAGCCCGGGGACGAGACCGTATTAGACGAAGTCGCGGAGGACTTGATAAACTTCATGAAGTACTCTGACGCCAAAG
TTTTGGTAATTTACCCCCACTCCAAGCTATCTCCAATGCCGGAGAACTACCACGAAGCTATGAAGAAGATACGGGAGTTG
GTAGATCTCTTGCAGAGGAAGGGAGTGCCGGTAAAGGAGGTGGAGGCCGGCGCTAAGGGGTTCGTACTAGAGACCTTGGG
CGTCCCGGCCCTTCACGAGTTCCGCTCCTACGGCCCCCGGAAGAGGCTTCAGACGGTCGCGGAGTACGTGGTGAACCCAG
AAGGGGAGTTCAAGGGGATTGAGGAGTGCGAGGACGCCTCGTTGGTCAGCTTGATAAAGTTCATGAAGGGGGGCCAAAAG
GTAGAGCACGACCCCAACGTGGAGAGGTTCTGTCGCAAGTTCGGCTTCGCGGAGGCCGAGGTGACCCCAAGAGGCTTTTG
GACCCTCCTGCCCCACGCCTACTTCATGTACTTGGCCTTCACAGTCCACGTTACCTCCGTGCTCCAAGCTTTGGAGGTGC
CCACGATCTCCGTCAAGACGCCGGAGGTGGTTAGGTACGGCTGTGACATCTCTTGTTCAGACGGCTTCTTGAGGTGCGAC
GAGTTCAAAGAACACAAACAAGTTGTGGAGAAGTACGCGGACCCCAACGACTTACCCTTCGCCCTCTTTGAGCAGCTCTT
GTTGTTTAGGGAAGAGGAAGACACCTTCCCGTGCTACAGAACGAAAGAGTTCCACGTCCCTAAGGTAAGAATATTCGTTC
CCTCCTTCTCCGAGGCCTCCGACGTTGCGCTCACCCTGCACGAGCTCATCCACACGGAGGCGGAGAAGCTCGGGATGAAG
TACGTAGTCTCCTACACAGTTTCTGAGGAGCTCTTCGAAGAGGCCAAGGACTTGATCATAAAGCTGGTTAAGAGGGACAA
CCGCTGCGCGTACCTAAGGGTGGTCAAATCCAATGAGATCTTCATCGACGCCGAAATGTACGTGATAAACTCCATGGGGT
ACCCCGTGGAGCTGGGGACGTGGAAGATGAGGAAGGCGCTCATGTGGGGCAGGGAGGTCTACTCCGTCTCTGCCGCCCCC
CTAGGCTCCTTCGAGCGCTTCGCTTACGTGATATTCGACAAGGCCGCCAAGGACTTGAAGTCCGGCGAGACGCCGGAGCT
TCCGGCCTGGCTCTCCCCCATACAAGTTAGGGTCATACCTAAGAGCAGGGACGTACTGCCCTACGCGCTCACCGTTGCGG
AGGAGCTGAGGGAGAGGGGGATAAGGGTGGATGTAGACGACAGGAACGTGCCCCTCTCCAGAAAGCTGAAGGACGCGGGA
GAGGAGTGGGTGCCGTTCCTACTCTTAATAGACAAGAGGGAAGAGAAGCTCGGGAGGGTCATACTGATGAGGAGGAAGAC
CAACGATCGGGAGGACGTGAGCTTAGAGGAAGTAATAAGGGTGGTCTCCGAGGAAGTTGGGGGCTACCCCCAGCTCCCCC
AGACGCTCCCGATACTGTACTCCAAGAGGCCCAAGTTCGCCAAGCCCGATGAGGACTAG

Upstream 100 bases:

>100_bases
GATAGGTTCCCTCATAGGGGCTGTAGCCTACAAGGTCCCCCGCGCGGTTTCTCCAAAACTATAATTCGTCCTACGCTTAG
GCTATAACGGAGACTAAGTA

Downstream 100 bases:

>100_bases
GGCAGTATCTGAGGAGGTGAAGTAGGTCTTGAGCGCTGAGGGAATCTCCTTCAAACCCACCTTGAGGGCCAGAGCGGGGG
AGCTGTTCTTCCTAGCGCTG

Product: anticodon-binding domain-containing protein

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS [H]

Number of amino acids: Translated: 552; Mature: 552

Protein sequence:

>552_residues
MKVLIVRSKSFAAGNERLDDALVAFVAVEPGDETVLDEVAEDLINFMKYSDAKVLVIYPHSKLSPMPENYHEAMKKIREL
VDLLQRKGVPVKEVEAGAKGFVLETLGVPALHEFRSYGPRKRLQTVAEYVVNPEGEFKGIEECEDASLVSLIKFMKGGQK
VEHDPNVERFCRKFGFAEAEVTPRGFWTLLPHAYFMYLAFTVHVTSVLQALEVPTISVKTPEVVRYGCDISCSDGFLRCD
EFKEHKQVVEKYADPNDLPFALFEQLLLFREEEDTFPCYRTKEFHVPKVRIFVPSFSEASDVALTLHELIHTEAEKLGMK
YVVSYTVSEELFEEAKDLIIKLVKRDNRCAYLRVVKSNEIFIDAEMYVINSMGYPVELGTWKMRKALMWGREVYSVSAAP
LGSFERFAYVIFDKAAKDLKSGETPELPAWLSPIQVRVIPKSRDVLPYALTVAEELRERGIRVDVDDRNVPLSRKLKDAG
EEWVPFLLLIDKREEKLGRVILMRRKTNDREDVSLEEVIRVVSEEVGGYPQLPQTLPILYSKRPKFAKPDED

Sequences:

>Translated_552_residues
MKVLIVRSKSFAAGNERLDDALVAFVAVEPGDETVLDEVAEDLINFMKYSDAKVLVIYPHSKLSPMPENYHEAMKKIREL
VDLLQRKGVPVKEVEAGAKGFVLETLGVPALHEFRSYGPRKRLQTVAEYVVNPEGEFKGIEECEDASLVSLIKFMKGGQK
VEHDPNVERFCRKFGFAEAEVTPRGFWTLLPHAYFMYLAFTVHVTSVLQALEVPTISVKTPEVVRYGCDISCSDGFLRCD
EFKEHKQVVEKYADPNDLPFALFEQLLLFREEEDTFPCYRTKEFHVPKVRIFVPSFSEASDVALTLHELIHTEAEKLGMK
YVVSYTVSEELFEEAKDLIIKLVKRDNRCAYLRVVKSNEIFIDAEMYVINSMGYPVELGTWKMRKALMWGREVYSVSAAP
LGSFERFAYVIFDKAAKDLKSGETPELPAWLSPIQVRVIPKSRDVLPYALTVAEELRERGIRVDVDDRNVPLSRKLKDAG
EEWVPFLLLIDKREEKLGRVILMRRKTNDREDVSLEEVIRVVSEEVGGYPQLPQTLPILYSKRPKFAKPDED
>Mature_552_residues
MKVLIVRSKSFAAGNERLDDALVAFVAVEPGDETVLDEVAEDLINFMKYSDAKVLVIYPHSKLSPMPENYHEAMKKIREL
VDLLQRKGVPVKEVEAGAKGFVLETLGVPALHEFRSYGPRKRLQTVAEYVVNPEGEFKGIEECEDASLVSLIKFMKGGQK
VEHDPNVERFCRKFGFAEAEVTPRGFWTLLPHAYFMYLAFTVHVTSVLQALEVPTISVKTPEVVRYGCDISCSDGFLRCD
EFKEHKQVVEKYADPNDLPFALFEQLLLFREEEDTFPCYRTKEFHVPKVRIFVPSFSEASDVALTLHELIHTEAEKLGMK
YVVSYTVSEELFEEAKDLIIKLVKRDNRCAYLRVVKSNEIFIDAEMYVINSMGYPVELGTWKMRKALMWGREVYSVSAAP
LGSFERFAYVIFDKAAKDLKSGETPELPAWLSPIQVRVIPKSRDVLPYALTVAEELRERGIRVDVDDRNVPLSRKLKDAG
EEWVPFLLLIDKREEKLGRVILMRRKTNDREDVSLEEVIRVVSEEVGGYPQLPQTLPILYSKRPKFAKPDED

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

None

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002320
- InterPro:   IPR015011
- ProDom:   PD016189 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF08915 tRNA-Thr_ED [H]

EC number: =6.1.1.3 [H]

Molecular weight: Translated: 63164; Mature: 63164

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLIVRSKSFAAGNERLDDALVAFVAVEPGDETVLDEVAEDLINFMKYSDAKVLVIYPH
CEEEEEECCCCCCCCHHHHHHEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEEECC
SKLSPMPENYHEAMKKIRELVDLLQRKGVPVKEVEAGAKGFVLETLGVPALHEFRSYGPR
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCEEEHHCCCCHHHHHHCCCHH
KRLQTVAEYVVNPEGEFKGIEECEDASLVSLIKFMKGGQKVEHDPNVERFCRKFGFAEAE
HHHHHHHHHHCCCCCCCCCCHHHCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCC
VTPRGFWTLLPHAYFMYLAFTVHVTSVLQALEVPTISVKTPEVVRYGCDISCSDGFLRCD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHCCCCEECCCCCEEHH
EFKEHKQVVEKYADPNDLPFALFEQLLLFREEEDTFPCYRTKEFHVPKVRIFVPSFSEAS
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCH
DVALTLHELIHTEAEKLGMKYVVSYTVSEELFEEAKDLIIKLVKRDNRCAYLRVVKSNEI
HHHHHHHHHHHHHHHHCCHHHEEEHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCEE
FIDAEMYVINSMGYPVELGTWKMRKALMWGREVYSVSAAPLGSFERFAYVIFDKAAKDLK
EEEEEEEEEECCCCCEEECHHHHHHHHHHCHHHHEECCCCCCCHHHHHHHHHHHHHHHHH
SGETPELPAWLSPIQVRVIPKSRDVLPYALTVAEELRERGIRVDVDDRNVPLSRKLKDAG
CCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHCC
EEWVPFLLLIDKREEKLGRVILMRRKTNDREDVSLEEVIRVVSEEVGGYPQLPQTLPILY
HHHCEEEEEEECCHHHHCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHH
SKRPKFAKPDED
CCCCCCCCCCCC
>Mature Secondary Structure
MKVLIVRSKSFAAGNERLDDALVAFVAVEPGDETVLDEVAEDLINFMKYSDAKVLVIYPH
CEEEEEECCCCCCCCHHHHHHEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEEECC
SKLSPMPENYHEAMKKIRELVDLLQRKGVPVKEVEAGAKGFVLETLGVPALHEFRSYGPR
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCEEEHHCCCCHHHHHHCCCHH
KRLQTVAEYVVNPEGEFKGIEECEDASLVSLIKFMKGGQKVEHDPNVERFCRKFGFAEAE
HHHHHHHHHHCCCCCCCCCCHHHCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCC
VTPRGFWTLLPHAYFMYLAFTVHVTSVLQALEVPTISVKTPEVVRYGCDISCSDGFLRCD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHCCCCEECCCCCEEHH
EFKEHKQVVEKYADPNDLPFALFEQLLLFREEEDTFPCYRTKEFHVPKVRIFVPSFSEAS
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCH
DVALTLHELIHTEAEKLGMKYVVSYTVSEELFEEAKDLIIKLVKRDNRCAYLRVVKSNEI
HHHHHHHHHHHHHHHHCCHHHEEEHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCEE
FIDAEMYVINSMGYPVELGTWKMRKALMWGREVYSVSAAPLGSFERFAYVIFDKAAKDLK
EEEEEEEEEECCCCCEEECHHHHHHHHHHCHHHHEECCCCCCCHHHHHHHHHHHHHHHHH
SGETPELPAWLSPIQVRVIPKSRDVLPYALTVAEELRERGIRVDVDDRNVPLSRKLKDAG
CCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHCC
EEWVPFLLLIDKREEKLGRVILMRRKTNDREDVSLEEVIRVVSEEVGGYPQLPQTLPILY
HHHCEEEEEEECCHHHHCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHH
SKRPKFAKPDED
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA