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The map label for this gene is livJ [C]

Identifier: 156936935

GI number: 156936935

Start: 126575

End: 127870

Strand: Reverse

Name: livJ [C]

Synonym: Igni_0140

Alternate gene names: 156936935

Gene position: 127870-126575 (Counterclockwise)

Preceding gene: 156936940

Following gene: 156936934

Centisome position: 9.85

GC content: 54.78

Gene sequence:

>1296_bases
GTGAGGACGGGTTTACTGTTAACGCTATTAATGACGGCCTTTGTAATCGCAGCTTCGACCGAAGAGATGGACCTGTTGAC
CAAGCCCTACCAACTGGGGCCCAACTGCGAGAGCATAAAGAAGGTGATAGAATGGGCCGGCGGCGTAGACAACATACGCC
TCCCGAAGGAGATACCGATAGGCGCACCCGCCCCGCTCAGCGGGGCCTTGGGTTCGTTCGCCGTCTCTAACTACAATGCA
ATTAAGATGGCCTCCGACGACATCAACGGCTGTATGGAGAAGCTCGGCCTTCCGTGGAGGTTCAAGTTCATAATGGAAGA
CACCCGCACAGACCCCCAAGTCGCTGTAGAAGTCGTTAGGAAGCTCGCTCTCCAAGGAGTACAAATAATTGCGGGCAGGA
TCACGAGCAGACCTATGGGGTCGTTGATGTATCCGGTCAACTCGGAGCTACACGTAGTGGTGATGTCCGCTACCAGCACC
GCGCCCAACTTAAGGATCGCCAACTACAAGGAGTACATCTCCAAGACCGGGAAGTCCTACATATTCTGGGCCATCACCAA
CGACTACTTCCAAGGGAAGGCGTTAGCCGAACTGGCCACCAAGCTCGGCTACAAGAAGGTCACTATCGTGTTCCGCCAAG
ACGACTACGGCGTTGGGCTGGCTAACGCCTTCAAGTATTACTTCTCTCAAAACGGCGGCGAATGCGTACTGGTCCCCTAC
GACCCTAACGCGAGGACCTTCGACGCGGAGCTGAGAGTAGTTGCCAACAGCAACCCCGACGCGATACTCTTCATCTCATT
CGACGAGCTGAAGGTTATATTGAGGCAAGCGATGCAACTAGGTTTGCAGAGGTACCCGTGGTTGTTCAGCGAGACTTGTA
GGGGCGTGCTCCTGAACGACCCGTACCTAGCCAAAGTAGTGGCCCTCAAGAACGGCATGGGCACCGGGCCCGGCGGCAGC
GACGTCTTCGCAAAGCTGTACGAAAAGAGGTTCGGCATACCTCCAATGTTGTACGCGGACTACATATATGACACCTTGTG
GCTGTCTGCCCTCAGCGTATTGAAGGCGAAGAAGTACGACGCCGACGCCATAAGGGAGGCGCTGATCGAGGTCGGAGCGT
ACTTCAAGGGCTATACCGGACCCAAGACCTTCAACCCGCTGACTCAAGAAGTTATGAGGTCCTCATACCACTACTGGACT
ATCAAGATAGAGAACGGCAAGCCGACCGTGGTCAAAGTAGGGTTCTGGGACCCCGAGAAAGGAATAGAACTCTTTACGCC
GATCAGGCCCAAGTAA

Upstream 100 bases:

>100_bases
TATATATTGATCATATACAGTTATGTACAATGCTATGTATGTTAGGGGTGCATATACTCAAAAACCCGGTCTGTGCAGCG
AAAGGGTGAGGGTGTAACCA

Downstream 100 bases:

>100_bases
AATTAAAATTTTTAAACCGAGGTTTACTCCCAAGGCTTTGGAGACTGTGCCATGAAGAAGTTTCTTCCTCTGCTCTTACT
AGCCGTAATGACGTCCGCAG

Product: extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MRTGLLLTLLMTAFVIAASTEEMDLLTKPYQLGPNCESIKKVIEWAGGVDNIRLPKEIPIGAPAPLSGALGSFAVSNYNA
IKMASDDINGCMEKLGLPWRFKFIMEDTRTDPQVAVEVVRKLALQGVQIIAGRITSRPMGSLMYPVNSELHVVVMSATST
APNLRIANYKEYISKTGKSYIFWAITNDYFQGKALAELATKLGYKKVTIVFRQDDYGVGLANAFKYYFSQNGGECVLVPY
DPNARTFDAELRVVANSNPDAILFISFDELKVILRQAMQLGLQRYPWLFSETCRGVLLNDPYLAKVVALKNGMGTGPGGS
DVFAKLYEKRFGIPPMLYADYIYDTLWLSALSVLKAKKYDADAIREALIEVGAYFKGYTGPKTFNPLTQEVMRSSYHYWT
IKIENGKPTVVKVGFWDPEKGIELFTPIRPK

Sequences:

>Translated_431_residues
MRTGLLLTLLMTAFVIAASTEEMDLLTKPYQLGPNCESIKKVIEWAGGVDNIRLPKEIPIGAPAPLSGALGSFAVSNYNA
IKMASDDINGCMEKLGLPWRFKFIMEDTRTDPQVAVEVVRKLALQGVQIIAGRITSRPMGSLMYPVNSELHVVVMSATST
APNLRIANYKEYISKTGKSYIFWAITNDYFQGKALAELATKLGYKKVTIVFRQDDYGVGLANAFKYYFSQNGGECVLVPY
DPNARTFDAELRVVANSNPDAILFISFDELKVILRQAMQLGLQRYPWLFSETCRGVLLNDPYLAKVVALKNGMGTGPGGS
DVFAKLYEKRFGIPPMLYADYIYDTLWLSALSVLKAKKYDADAIREALIEVGAYFKGYTGPKTFNPLTQEVMRSSYHYWT
IKIENGKPTVVKVGFWDPEKGIELFTPIRPK
>Mature_431_residues
MRTGLLLTLLMTAFVIAASTEEMDLLTKPYQLGPNCESIKKVIEWAGGVDNIRLPKEIPIGAPAPLSGALGSFAVSNYNA
IKMASDDINGCMEKLGLPWRFKFIMEDTRTDPQVAVEVVRKLALQGVQIIAGRITSRPMGSLMYPVNSELHVVVMSATST
APNLRIANYKEYISKTGKSYIFWAITNDYFQGKALAELATKLGYKKVTIVFRQDDYGVGLANAFKYYFSQNGGECVLVPY
DPNARTFDAELRVVANSNPDAILFISFDELKVILRQAMQLGLQRYPWLFSETCRGVLLNDPYLAKVVALKNGMGTGPGGS
DVFAKLYEKRFGIPPMLYADYIYDTLWLSALSVLKAKKYDADAIREALIEVGAYFKGYTGPKTFNPLTQEVMRSSYHYWT
IKIENGKPTVVKVGFWDPEKGIELFTPIRPK

Specific function: This Protein Is A Component Of The Leucine, Isoleucine, Valine, (Threonine) Transport System, Which Is One Of The Two Periplasmic Binding Protein-Dependent Transport Systems Of The High-Affinity Transport Of The Branched-Chain Amino Acids. [C]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 10140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 8822 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal med

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 48044; Mature: 48044

Theoretical pI: Translated: 9.14; Mature: 9.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTGLLLTLLMTAFVIAASTEEMDLLTKPYQLGPNCESIKKVIEWAGGVDNIRLPKEIPI
CCCHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCC
GAPAPLSGALGSFAVSNYNAIKMASDDINGCMEKLGLPWRFKFIMEDTRTDPQVAVEVVR
CCCCCCCCCHHHHHHCCCCEEEECCCHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHH
KLALQGVQIIAGRITSRPMGSLMYPVNSELHVVVMSATSTAPNLRIANYKEYISKTGKSY
HHHHCCHHHHHHHHCCCCCCCEECCCCCCEEEEEEEECCCCCCCEECCHHHHHHHCCCCE
IFWAITNDYFQGKALAELATKLGYKKVTIVFRQDDYGVGLANAFKYYFSQNGGECVLVPY
EEEEEECCCCCCHHHHHHHHHCCCEEEEEEEEECCCCCCHHHHHHHHHCCCCCEEEEEEC
DPNARTFDAELRVVANSNPDAILFISFDELKVILRQAMQLGLQRYPWLFSETCRGVLLND
CCCCCEEEEEEEEEECCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCHHHHCCCCEEECC
PYLAKVVALKNGMGTGPGGSDVFAKLYEKRFGIPPMLYADYIYDTLWLSALSVLKAKKYD
CHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
ADAIREALIEVGAYFKGYTGPKTFNPLTQEVMRSSYHYWTIKIENGKPTVVKVGFWDPEK
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCEEEEEEEECCCCCEEEEECCCCCC
GIELFTPIRPK
CCEECCCCCCC
>Mature Secondary Structure
MRTGLLLTLLMTAFVIAASTEEMDLLTKPYQLGPNCESIKKVIEWAGGVDNIRLPKEIPI
CCCHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCC
GAPAPLSGALGSFAVSNYNAIKMASDDINGCMEKLGLPWRFKFIMEDTRTDPQVAVEVVR
CCCCCCCCCHHHHHHCCCCEEEECCCHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHH
KLALQGVQIIAGRITSRPMGSLMYPVNSELHVVVMSATSTAPNLRIANYKEYISKTGKSY
HHHHCCHHHHHHHHCCCCCCCEECCCCCCEEEEEEEECCCCCCCEECCHHHHHHHCCCCE
IFWAITNDYFQGKALAELATKLGYKKVTIVFRQDDYGVGLANAFKYYFSQNGGECVLVPY
EEEEEECCCCCCHHHHHHHHHCCCEEEEEEEEECCCCCCHHHHHHHHHCCCCCEEEEEEC
DPNARTFDAELRVVANSNPDAILFISFDELKVILRQAMQLGLQRYPWLFSETCRGVLLND
CCCCCEEEEEEEEEECCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCHHHHCCCCEEECC
PYLAKVVALKNGMGTGPGGSDVFAKLYEKRFGIPPMLYADYIYDTLWLSALSVLKAKKYD
CHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
ADAIREALIEVGAYFKGYTGPKTFNPLTQEVMRSSYHYWTIKIENGKPTVVKVGFWDPEK
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCEEEEEEEECCCCCEEEEECCCCCC
GIELFTPIRPK
CCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]