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The map label for this gene is endA [H]

Identifier: 156936915

GI number: 156936915

Start: 108432

End: 108974

Strand: Reverse

Name: endA [H]

Synonym: Igni_0120

Alternate gene names: 156936915

Gene position: 108974-108432 (Counterclockwise)

Preceding gene: 156936916

Following gene: 156936914

Centisome position: 8.4

GC content: 55.25

Gene sequence:

>543_bases
TTGTACACCGCCTACTTGGTAGGCGACAGAGTGGTAGTGCTGGACGACGCCGCTGCCAAGGACATATACTCGAAGGGCTT
CTTCGGGCACCCCTTGGGGACGGAGAAGCCAAAGGGCCCGGAGGACGTGAAAGCGCCTTTGGCGCTCTCGCCGTTGGAGG
CACTCTACTTGCTAGAGACCGGGGTCATTGAGATACGTGACATAACGACCGACAAGAGCTACAGCCCAGAGGAGCTCAAA
GAGATTTGGAAATTCATGGAGGGGTTGGAAGAGAAGTACCTCGTTTACAAGGAATTGAGAAACAAGGGCTTCGTCGTGAG
GTCGGGGCTGAAGTACGGCTCCGACTTCGTCGTGTACGAGTTCGGGCCCGGGATAGACCACGCCCCCTACGTCGTGGACG
TATTGGAGAAGGGCACCAGCTTGGACCCGTCAGAGATAGTGAAGGGGGGTAGGGTGGCGCACGGGGTGAGGAAGAGATAC
ATAATGGCAATAGTAGAGAACGGCTCTATAAACTACGTTATGCTTAAATGGTACTTGCCGTAG

Upstream 100 bases:

>100_bases
CCTCTCGTGGGAAATATTGGGCAACTTGGAAGACGCCTCCGACATACTAAAGGACGTAGCTGAGAACTTGAAATACTTCT
TAATGCATAAGGTGTAAACT

Downstream 100 bases:

>100_bases
GTACCCTCGACGAAAGGCTCCGGGCGAGGTGGGTGCTTAAGTTGAGGGACGGAGTAAGGAGGGGCGGCAAGGGGCGTAGA
GACCTCAAGCCGAAGCCTCT

Product: tRNA intron endonuclease

Products: NA

Alternate protein names: tRNA-intron endonuclease [H]

Number of amino acids: Translated: 180; Mature: 180

Protein sequence:

>180_residues
MYTAYLVGDRVVVLDDAAAKDIYSKGFFGHPLGTEKPKGPEDVKAPLALSPLEALYLLETGVIEIRDITTDKSYSPEELK
EIWKFMEGLEEKYLVYKELRNKGFVVRSGLKYGSDFVVYEFGPGIDHAPYVVDVLEKGTSLDPSEIVKGGRVAHGVRKRY
IMAIVENGSINYVMLKWYLP

Sequences:

>Translated_180_residues
MYTAYLVGDRVVVLDDAAAKDIYSKGFFGHPLGTEKPKGPEDVKAPLALSPLEALYLLETGVIEIRDITTDKSYSPEELK
EIWKFMEGLEEKYLVYKELRNKGFVVRSGLKYGSDFVVYEFGPGIDHAPYVVDVLEKGTSLDPSEIVKGGRVAHGVRKRY
IMAIVENGSINYVMLKWYLP
>Mature_180_residues
MYTAYLVGDRVVVLDDAAAKDIYSKGFFGHPLGTEKPKGPEDVKAPLALSPLEALYLLETGVIEIRDITTDKSYSPEELK
EIWKFMEGLEEKYLVYKELRNKGFVVRSGLKYGSDFVVYEFGPGIDHAPYVVDVLEKGTSLDPSEIVKGGRVAHGVRKRY
IMAIVENGSINYVMLKWYLP

Specific function: Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate

COG id: COG1676

COG function: function code J; tRNA splicing endonuclease

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA-intron endonuclease family. Archaeal short subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011856
- InterPro:   IPR006676
- InterPro:   IPR006677
- InterPro:   IPR006678
- InterPro:   IPR016442 [H]

Pfam domain/function: PF01974 tRNA_int_endo; PF02778 tRNA_int_endo_N [H]

EC number: =3.1.27.9 [H]

Molecular weight: Translated: 20244; Mature: 20244

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYTAYLVGDRVVVLDDAAAKDIYSKGFFGHPLGTEKPKGPEDVKAPLALSPLEALYLLET
CEEEEEECCEEEEECCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEECHHHHHHHHHC
GVIEIRDITTDKSYSPEELKEIWKFMEGLEEKYLVYKELRNKGFVVRSGLKYGSDFVVYE
CCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCEEEEEE
FGPGIDHAPYVVDVLEKGTSLDPSEIVKGGRVAHGVRKRYIMAIVENGSINYVMLKWYLP
ECCCCCCCCHHHHHHHCCCCCCHHHHHCCCHHHHHHHHEEEEEEEECCCCCEEEEEEECC
>Mature Secondary Structure
MYTAYLVGDRVVVLDDAAAKDIYSKGFFGHPLGTEKPKGPEDVKAPLALSPLEALYLLET
CEEEEEECCEEEEECCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEECHHHHHHHHHC
GVIEIRDITTDKSYSPEELKEIWKFMEGLEEKYLVYKELRNKGFVVRSGLKYGSDFVVYE
CCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCEEEEEE
FGPGIDHAPYVVDVLEKGTSLDPSEIVKGGRVAHGVRKRYIMAIVENGSINYVMLKWYLP
ECCCCCCCCHHHHHHHCCCCCCHHHHHCCCHHHHHHHHEEEEEEEECCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10382966 [H]