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The map label for this gene is gltX2

Identifier: 154248640

GI number: 154248640

Start: 5236631

End: 5238007

Strand: Reverse

Name: gltX2

Synonym: Xaut_4722

Alternate gene names: 154248640

Gene position: 5238007-5236631 (Counterclockwise)

Preceding gene: 154248641

Following gene: 154248637

Centisome position: 98.66

GC content: 72.04

Gene sequence:

>1377_bases
ATGTCCCGCTCCACTCCGTCCCGCTCCACTCCGTCCCGCCCCGTCCTGCGCTTCGCGCCGTCGCCTACGGGTCTCATCCA
TGTGGGCAATGCCCGCACCGCCCTCATCAATGCGCTGCTCGCCCGGCGCGCCGGCGGCACCTTCATCCTGCGCTTCGACG
ATACCGACGCCGCCCGCTCCCGCGCCGAATATGCCGACGCCATCGCGACGGACCTCGCGTGGCTGGGCATCCCGCCCGAC
CTCACCTTCCGCCAGTCGGACCGCATCGGCGAGTATGAGGCCGCAACCCAGCAACTGAAGGCCTCCGGGCGGCTCTATCC
CGCCTATGAGAGCGAGGACGAGCTGGAGCTGAAGCGCCGGCTGCAGCGGGCGCGTAGCCTGCCGCCGGTCTATGACCGCG
CGGCGCTGGCGCTCACCGCGGCGGATCGTGCCCGGCTGGAGGCCGAGGGCCGCCGCCCGCACTGGCGCTTCCGCCTCGAC
CATCGCGTGGTCGCCTGGGACGATGGCGTGCGCGGGCCGCAGCAGGTGGACACGGCGAGCCTGTCCGATCCGGTTCTGGT
GCGCGCTGACGGCAGCTTCCTCTATACGCTGCCGTCCGTGGTGGATGACCTCGCCATGGGCGTCACGGACGTGGTGCGCG
GCGAGGACCATGTGACCAACACGGCGGTCCAGATCGAGATTTTCGAGGCGCTGGGCGGGGTGGCTCCGCGCTTCGCCCAC
CATAATCTGCTCACCCTGCCGTCAGGCGAGGGACTGTCGAAGCGGCTCGGCCACCTGTCCCTTTCGGCGCTGCGCGAGGC
TGGGCAGGAGGCGCTGGCGGTCGCGGCGGCGGCCGTCCTCGTGGGCACCTCCCATGCGGTGGAGGCGGTGGAAAGCCTCG
AGGCGCTGGCGGGCATGGTGGACCTCGCCCACATCTCCCGCGCCCCGGCCCGGTTCGATCCTGACGATCTTGCCCAGCTC
ACCGCCCGCACCCTCCACATGATGCCCTTCGAGGCTGCGGCTTCGCGCCTCGCGGCGGCGGGCATCGGCGGCGGCGTGGC
CTTCTGGCTGGCGGTGCGGGGCAATCTCGCGCGGTTTTCGGAGGCGGCGGATTGGTGGGCTCTGGTGTCCCAGCCGGCGG
CGGGTGTGGTGGCGGAGTCCGATCGTACCTTCCTCGGCGAAGCGGCGGCCCTGCTGCCGCCGGAGCCCTGGGACGGGCAG
ACCTACGCGAACTGGATCAAGGCGGTGAAAGCGGCGAGCGGGCAGTCGGGCAAGGCTCTGTTCCACCCGCTGCGCCTCGC
CCTGACCGGTCTGGAGAAGGGACCGGAGCTAGCCGCCTTGCTGCCGCTGATGGGCCGTGAGCGGGTCGCGGGCCGGCTGG
GCGGCGGGGTGGCGTAG

Upstream 100 bases:

>100_bases
ACGGCCGGGCTTCTTTCTTTCCGGGGCGGGCCGAGGCCCGATCCTCTTCCGGGGCTTCCTCCCCATCGACGGATGGAACG
TGGCGGGCTATGGGCTTGCC

Downstream 100 bases:

>100_bases
GCCGGAGCGGGTCAGGCGCAACCATTCGGGCGGGGCGAGTGCGAAGGGTGCGAAGCGGCTCGGATCCCGGTTGGAAACTC
ATCAAGGCAAACTCGCAATC

Product: glutamyl-tRNA synthetase class Ic

Products: NA

Alternate protein names: Glutamate--tRNA ligase 2; GluRS 2

Number of amino acids: Translated: 458; Mature: 457

Protein sequence:

>458_residues
MSRSTPSRSTPSRPVLRFAPSPTGLIHVGNARTALINALLARRAGGTFILRFDDTDAARSRAEYADAIATDLAWLGIPPD
LTFRQSDRIGEYEAATQQLKASGRLYPAYESEDELELKRRLQRARSLPPVYDRAALALTAADRARLEAEGRRPHWRFRLD
HRVVAWDDGVRGPQQVDTASLSDPVLVRADGSFLYTLPSVVDDLAMGVTDVVRGEDHVTNTAVQIEIFEALGGVAPRFAH
HNLLTLPSGEGLSKRLGHLSLSALREAGQEALAVAAAAVLVGTSHAVEAVESLEALAGMVDLAHISRAPARFDPDDLAQL
TARTLHMMPFEAAASRLAAAGIGGGVAFWLAVRGNLARFSEAADWWALVSQPAAGVVAESDRTFLGEAAALLPPEPWDGQ
TYANWIKAVKAASGQSGKALFHPLRLALTGLEKGPELAALLPLMGRERVAGRLGGGVA

Sequences:

>Translated_458_residues
MSRSTPSRSTPSRPVLRFAPSPTGLIHVGNARTALINALLARRAGGTFILRFDDTDAARSRAEYADAIATDLAWLGIPPD
LTFRQSDRIGEYEAATQQLKASGRLYPAYESEDELELKRRLQRARSLPPVYDRAALALTAADRARLEAEGRRPHWRFRLD
HRVVAWDDGVRGPQQVDTASLSDPVLVRADGSFLYTLPSVVDDLAMGVTDVVRGEDHVTNTAVQIEIFEALGGVAPRFAH
HNLLTLPSGEGLSKRLGHLSLSALREAGQEALAVAAAAVLVGTSHAVEAVESLEALAGMVDLAHISRAPARFDPDDLAQL
TARTLHMMPFEAAASRLAAAGIGGGVAFWLAVRGNLARFSEAADWWALVSQPAAGVVAESDRTFLGEAAALLPPEPWDGQ
TYANWIKAVKAASGQSGKALFHPLRLALTGLEKGPELAALLPLMGRERVAGRLGGGVA
>Mature_457_residues
SRSTPSRSTPSRPVLRFAPSPTGLIHVGNARTALINALLARRAGGTFILRFDDTDAARSRAEYADAIATDLAWLGIPPDL
TFRQSDRIGEYEAATQQLKASGRLYPAYESEDELELKRRLQRARSLPPVYDRAALALTAADRARLEAEGRRPHWRFRLDH
RVVAWDDGVRGPQQVDTASLSDPVLVRADGSFLYTLPSVVDDLAMGVTDVVRGEDHVTNTAVQIEIFEALGGVAPRFAHH
NLLTLPSGEGLSKRLGHLSLSALREAGQEALAVAAAAVLVGTSHAVEAVESLEALAGMVDLAHISRAPARFDPDDLAQLT
ARTLHMMPFEAAASRLAAAGIGGGVAFWLAVRGNLARFSEAADWWALVSQPAAGVVAESDRTFLGEAAALLPPEPWDGQT
YANWIKAVKAASGQSGKALFHPLRLALTGLEKGPELAALLPLMGRERVAGRLGGGVA

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=488, Percent_Identity=28.4836065573771, Blast_Score=169, Evalue=4e-42,
Organism=Escherichia coli, GI1788743, Length=330, Percent_Identity=35.7575757575758, Blast_Score=184, Evalue=8e-48,
Organism=Escherichia coli, GI226510925, Length=249, Percent_Identity=31.3253012048193, Blast_Score=91, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI71993394, Length=475, Percent_Identity=27.7894736842105, Blast_Score=164, Evalue=9e-41,
Organism=Saccharomyces cerevisiae, GI6324540, Length=490, Percent_Identity=29.1836734693878, Blast_Score=165, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6321192, Length=219, Percent_Identity=27.8538812785388, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6324742, Length=106, Percent_Identity=30.188679245283, Blast_Score=67, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24665321, Length=331, Percent_Identity=32.9305135951662, Blast_Score=154, Evalue=1e-37,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE2_XANP2 (A7IPJ5)

Other databases:

- EMBL:   CP000781
- RefSeq:   YP_001419598.1
- ProteinModelPortal:   A7IPJ5
- SMR:   A7IPJ5
- STRING:   A7IPJ5
- GeneID:   5421482
- GenomeReviews:   CP000781_GR
- KEGG:   xau:Xaut_4722
- eggNOG:   COG0008
- HOGENOM:   HBG628189
- ProtClustDB:   CLSK2793726
- BioCyc:   XAUT78245:XAUT_4722-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 48931; Mature: 48800

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 254-254

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRSTPSRSTPSRPVLRFAPSPTGLIHVGNARTALINALLARRAGGTFILRFDDTDAARS
CCCCCCCCCCCCCCEEEECCCCCCEEEECCHHHHHHHHHHHHCCCCEEEEEECCCHHHHH
RAEYADAIATDLAWLGIPPDLTFRQSDRIGEYEAATQQLKASGRLYPAYESEDELELKRR
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEECCCCCCHHHHHHHH
LQRARSLPPVYDRAALALTAADRARLEAEGRRPHWRFRLDHRVVAWDDGVRGPQQVDTAS
HHHHHCCCCCHHHHHHEEEHHHHHHHHCCCCCCCEEEEECCEEEEECCCCCCCHHCCCCC
LSDPVLVRADGSFLYTLPSVVDDLAMGVTDVVRGEDHVTNTAVQIEIFEALGGVAPRFAH
CCCCEEEEECCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHCCCCHHHHC
HNLLTLPSGEGLSKRLGHLSLSALREAGQEALAVAAAAVLVGTSHAVEAVESLEALAGMV
CCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
DLAHISRAPARFDPDDLAQLTARTLHMMPFEAAASRLAAAGIGGGVAFWLAVRGNLARFS
HHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHH
EAADWWALVSQPAAGVVAESDRTFLGEAAALLPPEPWDGQTYANWIKAVKAASGQSGKAL
HHHHHHHHHCCCCCCEEECCCCHHHHCHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCHH
FHPLRLALTGLEKGPELAALLPLMGRERVAGRLGGGVA
HHHHHHHHHHCCCCCHHHHHHHHHCHHHHHHHCCCCCC
>Mature Secondary Structure 
SRSTPSRSTPSRPVLRFAPSPTGLIHVGNARTALINALLARRAGGTFILRFDDTDAARS
CCCCCCCCCCCCCEEEECCCCCCEEEECCHHHHHHHHHHHHCCCCEEEEEECCCHHHHH
RAEYADAIATDLAWLGIPPDLTFRQSDRIGEYEAATQQLKASGRLYPAYESEDELELKRR
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEECCCCCCHHHHHHHH
LQRARSLPPVYDRAALALTAADRARLEAEGRRPHWRFRLDHRVVAWDDGVRGPQQVDTAS
HHHHHCCCCCHHHHHHEEEHHHHHHHHCCCCCCCEEEEECCEEEEECCCCCCCHHCCCCC
LSDPVLVRADGSFLYTLPSVVDDLAMGVTDVVRGEDHVTNTAVQIEIFEALGGVAPRFAH
CCCCEEEEECCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHCCCCHHHHC
HNLLTLPSGEGLSKRLGHLSLSALREAGQEALAVAAAAVLVGTSHAVEAVESLEALAGMV
CCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
DLAHISRAPARFDPDDLAQLTARTLHMMPFEAAASRLAAAGIGGGVAFWLAVRGNLARFS
HHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHH
EAADWWALVSQPAAGVVAESDRTFLGEAAALLPPEPWDGQTYANWIKAVKAASGQSGKAL
HHHHHHHHHCCCCCCEEECCCCHHHHCHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCHH
FHPLRLALTGLEKGPELAALLPLMGRERVAGRLGGGVA
HHHHHHHHHHCCCCCHHHHHHHHHCHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA