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The map label for this gene is cysS

Identifier: 154248635

GI number: 154248635

Start: 5230836

End: 5232224

Strand: Reverse

Name: cysS

Synonym: Xaut_4717

Alternate gene names: 154248635

Gene position: 5232224-5230836 (Counterclockwise)

Preceding gene: 154248637

Following gene: 154248634

Centisome position: 98.56

GC content: 66.81

Gene sequence:

>1389_bases
ATGGAGCTCAGGCTCTACAATACCTTGACCCGCTCGAAGGACACGTTGCGTCCGCTCGATCCCGCCAACGTGCGCATGTA
TGTCTGCGGCCCCACCGTCTACGACCACGCCCATATCGGCAATGCGCGGCCGGTGATCGTGTTCGACGTGCTGTTCCGCC
TGCTGCGCCGCCTCTATGGCGAAGGCCACGTCAAATACGTCCGCAACATCACCGACGTGGACGACAAGATCAACGCGCGC
GCCGCCGAGCGTGGCATCACCATCCGCGATCTCACCGAAGAGACCTACCGCTGGTTCCGCGAGGATACGGCGGCGCTGAA
CTGCCTGCGGCCCACGGTGGAGCCGCGCGCCACCGAGCACATTGCCGAGATGCGCACCCTCATCGAGAGTCTGGTCGCCT
CCGGCCATGCCTATGTGGCGGAAGAGCACGTGCTGTTCCATGTGCCCTCCATGCCGGACTATGGCCGCCTCTCCCGCCGC
CCGCTGGACGAGATGGTCGCCGGCGCCCGCGTGGACGTGGCGCCCTACAAGCGCGATCCCATGGACTTCGTGCTGTGGAA
GCCGAGCGAGGCCGGCATCCCCGGCTGGCCCTCGCCCTGCGGCATCGCCACACCCGGCCGTCCCGGCTGGCACATCGAGT
GCTCGGCCATGTCGTGGCGCCACCTGGGCGAGACCTTCGACATCCATGGCGGCGGCATCGACCTCGTGTTCCCGCACCAT
GAGAACGAGATCGCCCAGTCGCGCTGCGCCTTCCACACCGGCGTGATGGCGCAGATGTGGATGCACAACGGCTTCCTGAT
GCTGGAAGGCGAGAAGATGTCGAAGTCCCTCGGCAACTTCGTCACCATCCGCGAACTGCTCGCCGAATGGCCGGGCGAGG
TGCTGCGCCTCGCCATGCTCTCCACCCATTACCGCCAGCCCATCAACTGGACCCGGCAGGGCCTGGGCTTCGCCGCCAAG
ACCCTGGACAAGTGGTATCGCATCATCGGCGAGGCGGAGGCCGAGACCGGGGAGGGCAATCCGTTCGAGACCGAGATCGC
CGACCGGCTCGCCGACGACCTCAACACGCCGTCCGTCATCACCCACCTGCACCATCTTGCGGAAGTCGCCGAGCACGAGG
AGGCCTCGTCCGCCCTGCGCCGCCGCTTCAAAGGCGCGGCCAACCTGCTGGGTCTGCTCGGCGATACCGAAACCGGGTGG
CGCGCCCGGCAGAAGGAGGCCATCGCTTTGGACGAGGGCGTGATCGCCGGCCTCATCGCCGATCGCCTCGCCGCCCGCAA
GGCGAAGGATTTCAAGCGCGCCGACCAGATCCGCGAGGAGTTGGCCGCTCAGGGTGTGGTGCTGATGGACAACAAGGACG
GAACCACCAGCTGGGAGGTGTCGCGATGA

Upstream 100 bases:

>100_bases
GGCCGTGGCCGTCCCTTGCTGCTCCCTTAAGACTTAAGGATCTGGACGCCCCGGCCGGTGCACCGGCACGGCGCACGTGC
GCCATTCGGGGGAACGATCC

Downstream 100 bases:

>100_bases
CCGCCGCCAAGCTCTCCCTGCGCCCGTACCTGCCGGCCGATGCGCCGGTTCTGGCGGCCCTGTTCCGTGCCTCCATCGCC
GATCTGACCGGCGAGGATTA

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 462; Mature: 462

Protein sequence:

>462_residues
MELRLYNTLTRSKDTLRPLDPANVRMYVCGPTVYDHAHIGNARPVIVFDVLFRLLRRLYGEGHVKYVRNITDVDDKINAR
AAERGITIRDLTEETYRWFREDTAALNCLRPTVEPRATEHIAEMRTLIESLVASGHAYVAEEHVLFHVPSMPDYGRLSRR
PLDEMVAGARVDVAPYKRDPMDFVLWKPSEAGIPGWPSPCGIATPGRPGWHIECSAMSWRHLGETFDIHGGGIDLVFPHH
ENEIAQSRCAFHTGVMAQMWMHNGFLMLEGEKMSKSLGNFVTIRELLAEWPGEVLRLAMLSTHYRQPINWTRQGLGFAAK
TLDKWYRIIGEAEAETGEGNPFETEIADRLADDLNTPSVITHLHHLAEVAEHEEASSALRRRFKGAANLLGLLGDTETGW
RARQKEAIALDEGVIAGLIADRLAARKAKDFKRADQIREELAAQGVVLMDNKDGTTSWEVSR

Sequences:

>Translated_462_residues
MELRLYNTLTRSKDTLRPLDPANVRMYVCGPTVYDHAHIGNARPVIVFDVLFRLLRRLYGEGHVKYVRNITDVDDKINAR
AAERGITIRDLTEETYRWFREDTAALNCLRPTVEPRATEHIAEMRTLIESLVASGHAYVAEEHVLFHVPSMPDYGRLSRR
PLDEMVAGARVDVAPYKRDPMDFVLWKPSEAGIPGWPSPCGIATPGRPGWHIECSAMSWRHLGETFDIHGGGIDLVFPHH
ENEIAQSRCAFHTGVMAQMWMHNGFLMLEGEKMSKSLGNFVTIRELLAEWPGEVLRLAMLSTHYRQPINWTRQGLGFAAK
TLDKWYRIIGEAEAETGEGNPFETEIADRLADDLNTPSVITHLHHLAEVAEHEEASSALRRRFKGAANLLGLLGDTETGW
RARQKEAIALDEGVIAGLIADRLAARKAKDFKRADQIREELAAQGVVLMDNKDGTTSWEVSR
>Mature_462_residues
MELRLYNTLTRSKDTLRPLDPANVRMYVCGPTVYDHAHIGNARPVIVFDVLFRLLRRLYGEGHVKYVRNITDVDDKINAR
AAERGITIRDLTEETYRWFREDTAALNCLRPTVEPRATEHIAEMRTLIESLVASGHAYVAEEHVLFHVPSMPDYGRLSRR
PLDEMVAGARVDVAPYKRDPMDFVLWKPSEAGIPGWPSPCGIATPGRPGWHIECSAMSWRHLGETFDIHGGGIDLVFPHH
ENEIAQSRCAFHTGVMAQMWMHNGFLMLEGEKMSKSLGNFVTIRELLAEWPGEVLRLAMLSTHYRQPINWTRQGLGFAAK
TLDKWYRIIGEAEAETGEGNPFETEIADRLADDLNTPSVITHLHHLAEVAEHEEASSALRRRFKGAANLLGLLGDTETGW
RARQKEAIALDEGVIAGLIADRLAARKAKDFKRADQIREELAAQGVVLMDNKDGTTSWEVSR

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=384, Percent_Identity=38.0208333333333, Blast_Score=224, Evalue=1e-58,
Organism=Homo sapiens, GI10835051, Length=320, Percent_Identity=34.0625, Blast_Score=172, Evalue=6e-43,
Organism=Homo sapiens, GI62240992, Length=320, Percent_Identity=34.0625, Blast_Score=172, Evalue=8e-43,
Organism=Homo sapiens, GI21269877, Length=320, Percent_Identity=34.0625, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI303304987, Length=320, Percent_Identity=34.0625, Blast_Score=171, Evalue=1e-42,
Organism=Escherichia coli, GI1786737, Length=464, Percent_Identity=47.8448275862069, Blast_Score=381, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI17509795, Length=357, Percent_Identity=31.0924369747899, Blast_Score=153, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17509797, Length=357, Percent_Identity=31.0924369747899, Blast_Score=153, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6324082, Length=303, Percent_Identity=33.003300330033, Blast_Score=164, Evalue=2e-41,
Organism=Drosophila melanogaster, GI19922218, Length=380, Percent_Identity=36.3157894736842, Blast_Score=217, Evalue=2e-56,
Organism=Drosophila melanogaster, GI19922364, Length=235, Percent_Identity=40, Blast_Score=169, Evalue=4e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_XANP2 (A7IPJ0)

Other databases:

- EMBL:   CP000781
- RefSeq:   YP_001419593.1
- ProteinModelPortal:   A7IPJ0
- SMR:   A7IPJ0
- STRING:   A7IPJ0
- GeneID:   5425231
- GenomeReviews:   CP000781_GR
- KEGG:   xau:Xaut_4717
- eggNOG:   COG0215
- HOGENOM:   HBG327651
- OMA:   PEEMLAG
- ProtClustDB:   PRK00260
- BioCyc:   XAUT78245:XAUT_4717-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00041_B
- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- SMART:   SM00840
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 52086; Mature: 52086

Theoretical pI: Translated: 6.47; Mature: 6.47

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 275-275

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELRLYNTLTRSKDTLRPLDPANVRMYVCGPTVYDHAHIGNARPVIVFDVLFRLLRRLYG
CCEEEHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHC
EGHVKYVRNITDVDDKINARAAERGITIRDLTEETYRWFREDTAALNCLRPTVEPRATEH
CCCHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
IAEMRTLIESLVASGHAYVAEEHVLFHVPSMPDYGRLSRRPLDEMVAGARVDVAPYKRDP
HHHHHHHHHHHHHCCCCEEECCCEEEECCCCCCCCCHHCCCHHHHHCCCCEEECCCCCCC
MDFVLWKPSEAGIPGWPSPCGIATPGRPGWHIECSAMSWRHLGETFDIHGGGIDLVFPHH
CCEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHCCCEEEECCCCEEEEECCC
ENEIAQSRCAFHTGVMAQMWMHNGFLMLEGEKMSKSLGNFVTIRELLAEWPGEVLRLAML
CCHHHHHHHHHHHHHHHHHHHHCCEEEEECHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
STHYRQPINWTRQGLGFAAKTLDKWYRIIGEAEAETGEGNPFETEIADRLADDLNTPSVI
HHHHCCCCCHHHHHCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCHHHH
THLHHLAEVAEHEEASSALRRRFKGAANLLGLLGDTETGWRARQKEAIALDEGVIAGLIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCHHHHCCHHHHHHHH
DRLAARKAKDFKRADQIREELAAQGVVLMDNKDGTTSWEVSR
HHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCC
>Mature Secondary Structure
MELRLYNTLTRSKDTLRPLDPANVRMYVCGPTVYDHAHIGNARPVIVFDVLFRLLRRLYG
CCEEEHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHC
EGHVKYVRNITDVDDKINARAAERGITIRDLTEETYRWFREDTAALNCLRPTVEPRATEH
CCCHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
IAEMRTLIESLVASGHAYVAEEHVLFHVPSMPDYGRLSRRPLDEMVAGARVDVAPYKRDP
HHHHHHHHHHHHHCCCCEEECCCEEEECCCCCCCCCHHCCCHHHHHCCCCEEECCCCCCC
MDFVLWKPSEAGIPGWPSPCGIATPGRPGWHIECSAMSWRHLGETFDIHGGGIDLVFPHH
CCEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHCCCEEEECCCCEEEEECCC
ENEIAQSRCAFHTGVMAQMWMHNGFLMLEGEKMSKSLGNFVTIRELLAEWPGEVLRLAML
CCHHHHHHHHHHHHHHHHHHHHCCEEEEECHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
STHYRQPINWTRQGLGFAAKTLDKWYRIIGEAEAETGEGNPFETEIADRLADDLNTPSVI
HHHHCCCCCHHHHHCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCHHHH
THLHHLAEVAEHEEASSALRRRFKGAANLLGLLGDTETGWRARQKEAIALDEGVIAGLIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCHHHHCCHHHHHHHH
DRLAARKAKDFKRADQIREELAAQGVVLMDNKDGTTSWEVSR
HHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA