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The map label for this gene is braD [H]

Identifier: 154248071

GI number: 154248071

Start: 4592936

End: 4594573

Strand: Direct

Name: braD [H]

Synonym: Xaut_4150

Alternate gene names: 154248071

Gene position: 4592936-4594573 (Clockwise)

Preceding gene: 154248070

Following gene: 154248072

Centisome position: 86.51

GC content: 68.56

Gene sequence:

>1638_bases
ATGATGCGCCCTCCCGTTCCGCCGGCCCTCGCGCGGATCCTCGCGCTTCCCCTTCTCGCCCTTCTTCTGGCTGTCCTCCT
CCCCGCCTGCGCGGTGCGCGCGCAGGCACAGGCCGATATTTCGGCCATCGTCGCGAAATTCGCGAACGACAGCTATTCGG
ATACGACCGACGCGCTGGTTGAGCTTTCGGCCACCGGCAGCGCCCAGGCCGCCGCCGTGGTGGAGGCGCTCGGTGCCGGC
CGGCTGATGTTCGATCCGGCCACCAAGGCGGTCTATATCCGCACCCCCGCCGGCATGGCCGATGCTGCCACCGGCACCAT
CGTCGCCAATGCGCCGACCAACCTCTCCGTGGTGCGCGCCAACAACCGGGTGCGCCGCGCCATCGATGCGGCTTCCGGCG
CGCTCTCGCTGCTCAATCCCGATCCGGTCCGCCGCGCCGAGGCTGCAGCCGCCGTCTTCAAGTCGCGCGATGCCGCTGCG
CTGGCGACGGTGGAGGCCGCCATCGCCAAGGAGACGGTGGGAAGCGTGAAGGATGCGCTGGAGCAGGCCCGCGCCGCCAT
TCTCGTCGGCAAGTCCGAGGTGCCCGAGGCGGTGCGGCTCCTCGCCATCCAGAAGATCACCGCCCGCGGCGACCAGGAGG
CCATCAGCCTGCTGCGCAGCCTGCCGGCGGACGCCCCCGCCAGCCTCAAGGCCGCGGCCGAGGCCGGCATCGCGAAGGTG
GAGCAGAATCTCAAGTTCTGGGCCGTGGCGCAGAATGTCTGGTACGGCCTGTCGCTGGGCTCGGTGCTGCTTTTGGCGGC
CATCGGGCTGGCCATCACCTTCGGTGTCATGGGCGTCATCAACATGGCCCACGGCGAGATGGTGATGCTGGGTGCCTACA
CCACTTTCGTGGTGCAGGACTTCATCCGCCATTCGGCGCCCGGCCTGTTCGACTGGTCGCTGGCCATCGCCCTGCCGCTG
GCCTTCCTGTTCACCGGCCTCGTGGGCATCCTCATCGAGCGCACGGTGATCCGCTTCCTCTACGGCCGGCCGCTGGAAAC
CCTGCTCGCCACCTGGGGTGTCTCGCTCATCCTCCAGCAGGCGGTGCGGTCCGTGTTCGGCCCCACCAACCAGGAGGTGG
GGGCACCATCCTGGATGTCGGGCGCGTTCCAGGTGGGCCAGCTCTCCATCACCTATAACCGCCTGTGGATCATCGTCTTC
GCCCTGCTGGTGTTCTTCGCTCTGCTGCTGGTGCTGAAGCGCACGCCCCTCGGCCTCTATGTGCGCGCGGTGACGCAGAA
CCGGCGCATGGCGGCGGCCATGGGCATCCGCACCGGGCGCATCGACGCGCTCACCTTCGGCCTCGGCTCCGGCATCGCCG
GCATCGCGGGCGTGGCGCTCTCGCAGATCGACAATGTGAGCCCCAACCTCGGGCAGGGCTACATCATCGACAGCTTCATG
GTGGTGGTGTTCGGCGGGGTCGGGAACCTCTGGGGCACGCTGGTGGGCGCCTTCACGCTGGGTATCGCCAACAAGCTGCT
GGAGCCCTTCGCCGGCGCGGTGCTGGGCAAGATCCTGCTGCTCGTCTTCATCATCCTGTTCATCCAGAAGCGCCCGCGCG
GCCTGTTCGCGCTCAAGGGCCGGGCGGTGGAATCGTGA

Upstream 100 bases:

>100_bases
AGGGGCGCTCCTCCCTCTCCCCGGCAACGGGGAGGGGGAGCCTCGCTCTTCCCGTCGCGAGCTGCCGGCACCGCCCGGCT
GCGCTGGAGCGGATACCTTC

Downstream 100 bases:

>100_bases
AACAGTCCGGCATCATCGACCGCACCGGCGGCATCTTCCTTCTCGTCATGCTGGCGGTGGCGGTGCTGGTGCCCGCCTCC
AACCTGCTGCTCCCGGCCGG

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 545; Mature: 545

Protein sequence:

>545_residues
MMRPPVPPALARILALPLLALLLAVLLPACAVRAQAQADISAIVAKFANDSYSDTTDALVELSATGSAQAAAVVEALGAG
RLMFDPATKAVYIRTPAGMADAATGTIVANAPTNLSVVRANNRVRRAIDAASGALSLLNPDPVRRAEAAAAVFKSRDAAA
LATVEAAIAKETVGSVKDALEQARAAILVGKSEVPEAVRLLAIQKITARGDQEAISLLRSLPADAPASLKAAAEAGIAKV
EQNLKFWAVAQNVWYGLSLGSVLLLAAIGLAITFGVMGVINMAHGEMVMLGAYTTFVVQDFIRHSAPGLFDWSLAIALPL
AFLFTGLVGILIERTVIRFLYGRPLETLLATWGVSLILQQAVRSVFGPTNQEVGAPSWMSGAFQVGQLSITYNRLWIIVF
ALLVFFALLLVLKRTPLGLYVRAVTQNRRMAAAMGIRTGRIDALTFGLGSGIAGIAGVALSQIDNVSPNLGQGYIIDSFM
VVVFGGVGNLWGTLVGAFTLGIANKLLEPFAGAVLGKILLLVFIILFIQKRPRGLFALKGRAVES

Sequences:

>Translated_545_residues
MMRPPVPPALARILALPLLALLLAVLLPACAVRAQAQADISAIVAKFANDSYSDTTDALVELSATGSAQAAAVVEALGAG
RLMFDPATKAVYIRTPAGMADAATGTIVANAPTNLSVVRANNRVRRAIDAASGALSLLNPDPVRRAEAAAAVFKSRDAAA
LATVEAAIAKETVGSVKDALEQARAAILVGKSEVPEAVRLLAIQKITARGDQEAISLLRSLPADAPASLKAAAEAGIAKV
EQNLKFWAVAQNVWYGLSLGSVLLLAAIGLAITFGVMGVINMAHGEMVMLGAYTTFVVQDFIRHSAPGLFDWSLAIALPL
AFLFTGLVGILIERTVIRFLYGRPLETLLATWGVSLILQQAVRSVFGPTNQEVGAPSWMSGAFQVGQLSITYNRLWIIVF
ALLVFFALLLVLKRTPLGLYVRAVTQNRRMAAAMGIRTGRIDALTFGLGSGIAGIAGVALSQIDNVSPNLGQGYIIDSFM
VVVFGGVGNLWGTLVGAFTLGIANKLLEPFAGAVLGKILLLVFIILFIQKRPRGLFALKGRAVES
>Mature_545_residues
MMRPPVPPALARILALPLLALLLAVLLPACAVRAQAQADISAIVAKFANDSYSDTTDALVELSATGSAQAAAVVEALGAG
RLMFDPATKAVYIRTPAGMADAATGTIVANAPTNLSVVRANNRVRRAIDAASGALSLLNPDPVRRAEAAAAVFKSRDAAA
LATVEAAIAKETVGSVKDALEQARAAILVGKSEVPEAVRLLAIQKITARGDQEAISLLRSLPADAPASLKAAAEAGIAKV
EQNLKFWAVAQNVWYGLSLGSVLLLAAIGLAITFGVMGVINMAHGEMVMLGAYTTFVVQDFIRHSAPGLFDWSLAIALPL
AFLFTGLVGILIERTVIRFLYGRPLETLLATWGVSLILQQAVRSVFGPTNQEVGAPSWMSGAFQVGQLSITYNRLWIIVF
ALLVFFALLLVLKRTPLGLYVRAVTQNRRMAAAMGIRTGRIDALTFGLGSGIAGIAGVALSQIDNVSPNLGQGYIIDSFM
VVVFGGVGNLWGTLVGAFTLGIANKLLEPFAGAVLGKILLLVFIILFIQKRPRGLFALKGRAVES

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0559

COG function: function code E; Branched-chain amino acid ABC-type transport system, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789866, Length=305, Percent_Identity=31.4754098360656, Blast_Score=97, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851 [H]

Pfam domain/function: PF02653 BPD_transp_2 [H]

EC number: NA

Molecular weight: Translated: 57198; Mature: 57198

Theoretical pI: Translated: 10.42; Mature: 10.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRPPVPPALARILALPLLALLLAVLLPACAVRAQAQADISAIVAKFANDSYSDTTDALV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHE
ELSATGSAQAAAVVEALGAGRLMFDPATKAVYIRTPAGMADAATGTIVANAPTNLSVVRA
EECCCCCHHHHHHHHHHCCCCEEECCCCCEEEEECCCCCCCCCCCCEEECCCCCEEEEEC
NNRVRRAIDAASGALSLLNPDPVRRAEAAAAVFKSRDAAALATVEAAIAKETVGSVKDAL
CHHHHHHHHHHCCCEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
EQARAAILVGKSEVPEAVRLLAIQKITARGDQEAISLLRSLPADAPASLKAAAEAGIAKV
HHHHHEEEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
EQNLKFWAVAQNVWYGLSLGSVLLLAAIGLAITFGVMGVINMAHGEMVMLGAYTTFVVQD
HHCCHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHH
FIRHSAPGLFDWSLAIALPLAFLFTGLVGILIERTVIRFLYGRPLETLLATWGVSLILQQ
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
AVRSVFGPTNQEVGAPSWMSGAFQVGQLSITYNRLWIIVFALLVFFALLLVLKRTPLGLY
HHHHHCCCCCCCCCCCCHHCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCHHE
VRAVTQNRRMAAAMGIRTGRIDALTFGLGSGIAGIAGVALSQIDNVSPNLGQGYIIDSFM
EEEHHCCCHHHHHHCCCCCCHHEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
VVVFGGVGNLWGTLVGAFTLGIANKLLEPFAGAVLGKILLLVFIILFIQKRPRGLFALKG
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECC
RAVES
CCCCC
>Mature Secondary Structure
MMRPPVPPALARILALPLLALLLAVLLPACAVRAQAQADISAIVAKFANDSYSDTTDALV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHE
ELSATGSAQAAAVVEALGAGRLMFDPATKAVYIRTPAGMADAATGTIVANAPTNLSVVRA
EECCCCCHHHHHHHHHHCCCCEEECCCCCEEEEECCCCCCCCCCCCEEECCCCCEEEEEC
NNRVRRAIDAASGALSLLNPDPVRRAEAAAAVFKSRDAAALATVEAAIAKETVGSVKDAL
CHHHHHHHHHHCCCEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
EQARAAILVGKSEVPEAVRLLAIQKITARGDQEAISLLRSLPADAPASLKAAAEAGIAKV
HHHHHEEEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
EQNLKFWAVAQNVWYGLSLGSVLLLAAIGLAITFGVMGVINMAHGEMVMLGAYTTFVVQD
HHCCHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHH
FIRHSAPGLFDWSLAIALPLAFLFTGLVGILIERTVIRFLYGRPLETLLATWGVSLILQQ
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
AVRSVFGPTNQEVGAPSWMSGAFQVGQLSITYNRLWIIVFALLVFFALLLVLKRTPLGLY
HHHHHCCCCCCCCCCCCHHCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCHHE
VRAVTQNRRMAAAMGIRTGRIDALTFGLGSGIAGIAGVALSQIDNVSPNLGQGYIIDSFM
EEEHHCCCHHHHHHCCCCCCHHEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
VVVFGGVGNLWGTLVGAFTLGIANKLLEPFAGAVLGKILLLVFIILFIQKRPRGLFALKG
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECC
RAVES
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043; 2509433 [H]