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The map label for this gene is phaA [H]

Identifier: 154247868

GI number: 154247868

Start: 4366023

End: 4366616

Strand: Direct

Name: phaA [H]

Synonym: Xaut_3945

Alternate gene names: 154247868

Gene position: 4366023-4366616 (Clockwise)

Preceding gene: 154247867

Following gene: 154247869

Centisome position: 82.24

GC content: 68.52

Gene sequence:

>594_bases
ATGAGGCGCATCAAGGCGCTGCGCGCCGCGGCGATGATCCTCGCCGACCGCGATTTCGCCGCGGCGAACGGCCTTGAGCC
GCTGGCGCGGCTCGTCGCCTTCGGCGTCGGCGCGGTGGAGCCGGGCATGTTCGGCATCGGTCCGGTGCCGGCGGTGCGCC
AGGCGCTGGGGCGTGCGGGCTGGACGATCGCGGACGTCGAGCAGGTGGAAATCAACGAGGCCTTCGCCGCGATCGCCCTC
GCCGTCACCCGCGATCTCGGCCTTGCCGAAGAGATCGTCAATGTTGAAGGTGGGGCCATCGCCCACGGCCACCCCATCGG
CGCGAGCGGGGCGATCCTGACCACGCGGCTCATCCATTCGATGCAGCGCGACGGCCTCAAGCGTGGCATCGTTACCCTCT
GCATCGGCGGCGGCCAGGCATCGCGCTGGCGATCGAAACACTGGCCTGACAGACCGGTCCGACACCTGTGCGGGCTGCCG
GCTTCGGCCGCGATCTCGCGCGTGTGCTTTGAAAGTGGCCATCGTGCGCCCATTGATTCCGCGGCAGAAGAACGAGCCTC
CTGTTGGGGGCACAGCCCAAATCGTCGTTTCTAA

Upstream 100 bases:

>100_bases
AACGCCTGGCTTTGCTCCACACCAACGTCCGCAAGTACGGCACGATTTCGAACACCGTTGCGGAAGCGACCCGATTGGGC
GGGACGATCCGAAGGAAGGA

Downstream 100 bases:

>100_bases
CGCCGACACGGATGCCCGTTTGCCTTCCAGGCCATCCGCGCTATTCTTCGATTTAAGAAGAATTACAAAGCGGGAGGCCC
AAGCAAATGAGCGCCGGTTC

Product: thiolase

Products: NA

Alternate protein names: Acetoacetyl-CoA thiolase [H]

Number of amino acids: Translated: 197; Mature: 197

Protein sequence:

>197_residues
MRRIKALRAAAMILADRDFAAANGLEPLARLVAFGVGAVEPGMFGIGPVPAVRQALGRAGWTIADVEQVEINEAFAAIAL
AVTRDLGLAEEIVNVEGGAIAHGHPIGASGAILTTRLIHSMQRDGLKRGIVTLCIGGGQASRWRSKHWPDRPVRHLCGLP
ASAAISRVCFESGHRAPIDSAAEERASCWGHSPNRRF

Sequences:

>Translated_197_residues
MRRIKALRAAAMILADRDFAAANGLEPLARLVAFGVGAVEPGMFGIGPVPAVRQALGRAGWTIADVEQVEINEAFAAIAL
AVTRDLGLAEEIVNVEGGAIAHGHPIGASGAILTTRLIHSMQRDGLKRGIVTLCIGGGQASRWRSKHWPDRPVRHLCGLP
ASAAISRVCFESGHRAPIDSAAEERASCWGHSPNRRF
>Mature_197_residues
MRRIKALRAAAMILADRDFAAANGLEPLARLVAFGVGAVEPGMFGIGPVPAVRQALGRAGWTIADVEQVEINEAFAAIAL
AVTRDLGLAEEIVNVEGGAIAHGHPIGASGAILTTRLIHSMQRDGLKRGIVTLCIGGGQASRWRSKHWPDRPVRHLCGLP
ASAAISRVCFESGHRAPIDSAAEERASCWGHSPNRRF

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI148539872, Length=130, Percent_Identity=52.3076923076923, Blast_Score=142, Evalue=3e-34,
Organism=Homo sapiens, GI167614485, Length=131, Percent_Identity=47.3282442748092, Blast_Score=121, Evalue=4e-28,
Organism=Homo sapiens, GI4501853, Length=131, Percent_Identity=44.2748091603053, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI4557237, Length=133, Percent_Identity=41.3533834586466, Blast_Score=94, Evalue=9e-20,
Organism=Homo sapiens, GI194353979, Length=81, Percent_Identity=45.679012345679, Blast_Score=68, Evalue=5e-12,
Organism=Escherichia coli, GI1788554, Length=131, Percent_Identity=52.6717557251908, Blast_Score=132, Evalue=1e-32,
Organism=Escherichia coli, GI1787663, Length=133, Percent_Identity=51.1278195488722, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI87082165, Length=131, Percent_Identity=49.618320610687, Blast_Score=115, Evalue=2e-27,
Organism=Escherichia coli, GI48994986, Length=134, Percent_Identity=45.5223880597015, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1788683, Length=143, Percent_Identity=34.965034965035, Blast_Score=67, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI133906874, Length=131, Percent_Identity=48.8549618320611, Blast_Score=130, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI25147385, Length=133, Percent_Identity=41.3533834586466, Blast_Score=101, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17535917, Length=133, Percent_Identity=40.6015037593985, Blast_Score=96, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17535921, Length=122, Percent_Identity=40.9836065573771, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17551802, Length=149, Percent_Identity=28.8590604026846, Blast_Score=63, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6325229, Length=134, Percent_Identity=41.7910447761194, Blast_Score=88, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6322031, Length=129, Percent_Identity=38.7596899224806, Blast_Score=88, Evalue=8e-19,
Organism=Drosophila melanogaster, GI17648125, Length=131, Percent_Identity=50.381679389313, Blast_Score=125, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24655093, Length=130, Percent_Identity=49.2307692307692, Blast_Score=121, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24640423, Length=132, Percent_Identity=43.1818181818182, Blast_Score=94, Evalue=8e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.9 [H]

Molecular weight: Translated: 20878; Mature: 20878

Theoretical pI: Translated: 9.75; Mature: 9.75

Prosite motif: PS00737 THIOLASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRIKALRAAAMILADRDFAAANGLEPLARLVAFGVGAVEPGMFGIGPVPAVRQALGRAG
CCHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCC
WTIADVEQVEINEAFAAIALAVTRDLGLAEEIVNVEGGAIAHGHPIGASGAILTTRLIHS
CEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHH
MQRDGLKRGIVTLCIGGGQASRWRSKHWPDRPVRHLCGLPASAAISRVCFESGHRAPIDS
HHHHHHHHCEEEEEECCCCHHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHHCCCCCCCCH
AAEERASCWGHSPNRRF
HHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MRRIKALRAAAMILADRDFAAANGLEPLARLVAFGVGAVEPGMFGIGPVPAVRQALGRAG
CCHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCC
WTIADVEQVEINEAFAAIALAVTRDLGLAEEIVNVEGGAIAHGHPIGASGAILTTRLIHS
CEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHH
MQRDGLKRGIVTLCIGGGQASRWRSKHWPDRPVRHLCGLPASAAISRVCFESGHRAPIDS
HHHHHHHHCEEEEEECCCCHHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHHCCCCCCCCH
AAEERASCWGHSPNRRF
HHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8566717 [H]