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The map label for this gene is pdxJ

Identifier: 154247801

GI number: 154247801

Start: 4297917

End: 4298690

Strand: Direct

Name: pdxJ

Synonym: Xaut_3878

Alternate gene names: 154247801

Gene position: 4297917-4298690 (Clockwise)

Preceding gene: 154247800

Following gene: 154247802

Centisome position: 80.96

GC content: 73.39

Gene sequence:

>774_bases
ATGGCGGTGCTGAAGCCGGTGCGGCTCGGAGTGAACGTCGATCACGTGGCGACGGTGCGCAACGCGCGCGGCGGCGCCCT
TCCCGACCCGGTGCGCGCCGCCGCCCTCGCCAAGGCCGCCGGAGCCCATGGCATCACCGCCCACTTGCGCGAAGACCGCC
GCCACATCCGCGACGCGGACATGGAGCGGCTGAAGGCCGAGATCGACCTGCCGCTCAATTTCGAGATGGCGGCGACTGAC
GAGATGGTTGCCATCGCCTGCCGCGTCCGTCCCAACGCCTGCTGCCTGGTGCCCGAGCGGCGCGAGGAGCGCACCACCGA
GGGTGGGCTCGATGCTGCCGGCCAGCGGGCGGAGCTTGCGCCCCGGATCGCCCGGCTGAAGGCGGCGGGCATCCGCGTCT
CCCTGTTCATCGCGGCGGATCCGGCCCAGATCGCGGCGGCGGCCGAGCTCGGCGCGGACATCGTGGAACTGCACACCGGC
GCCTGGTGCGACGCGGTGACGGAAGGCCGGCATGTGGAGGCCGAGGCCGAGTTCGTGCGGCTGAAGGCCGGGGCGCGGCA
GGCGGCGGGGCTCGGGCTGGAGGTCCATGCGGGCCACGGCCTCGACTATGCCACCGCCGAGCGCATCGCCGCCTTCCCGC
AGATCGTGGAGCTGAACATCGGCCACTTCCTCATCGGCGAGGCCATTTTCGTGGGGCTCGACCAGGCCATTGCGCGCATG
AGGGCGGCCATCGCGGCGGGCCGGGCCGCCGTCGGGGACGGCGCGGCGGCATGA

Upstream 100 bases:

>100_bases
GCCCGCGAGGTGGTGGCCCAGGTGGAGCGCGTGAACGGCTGACCCGCTGCGAGCGGGGCGCGCCCATCCATAAGCCGCAG
CGGAGCAGGGAGCAGGGACG

Downstream 100 bases:

>100_bases
TCATCGGCATCGGCTCGGACTTCTCGGACGCCCGGCGCATCGCCCGCTCCATCGAGCGGTTCGGCGACCGGTTCCTCGAC
CGGGTGTTCACGCCTGGAGA

Product: pyridoxine 5'-phosphate synthase

Products: NA

Alternate protein names: PNP synthase

Number of amino acids: Translated: 257; Mature: 256

Protein sequence:

>257_residues
MAVLKPVRLGVNVDHVATVRNARGGALPDPVRAAALAKAAGAHGITAHLREDRRHIRDADMERLKAEIDLPLNFEMAATD
EMVAIACRVRPNACCLVPERREERTTEGGLDAAGQRAELAPRIARLKAAGIRVSLFIAADPAQIAAAAELGADIVELHTG
AWCDAVTEGRHVEAEAEFVRLKAGARQAAGLGLEVHAGHGLDYATAERIAAFPQIVELNIGHFLIGEAIFVGLDQAIARM
RAAIAAGRAAVGDGAAA

Sequences:

>Translated_257_residues
MAVLKPVRLGVNVDHVATVRNARGGALPDPVRAAALAKAAGAHGITAHLREDRRHIRDADMERLKAEIDLPLNFEMAATD
EMVAIACRVRPNACCLVPERREERTTEGGLDAAGQRAELAPRIARLKAAGIRVSLFIAADPAQIAAAAELGADIVELHTG
AWCDAVTEGRHVEAEAEFVRLKAGARQAAGLGLEVHAGHGLDYATAERIAAFPQIVELNIGHFLIGEAIFVGLDQAIARM
RAAIAAGRAAVGDGAAA
>Mature_256_residues
AVLKPVRLGVNVDHVATVRNARGGALPDPVRAAALAKAAGAHGITAHLREDRRHIRDADMERLKAEIDLPLNFEMAATDE
MVAIACRVRPNACCLVPERREERTTEGGLDAAGQRAELAPRIARLKAAGIRVSLFIAADPAQIAAAAELGADIVELHTGA
WCDAVTEGRHVEAEAEFVRLKAGARQAAGLGLEVHAGHGLDYATAERIAAFPQIVELNIGHFLIGEAIFVGLDQAIARMR
AAIAAGRAAVGDGAAA

Specific function: Catalyzes the complicated ring closure reaction between the two acyclic compounds 1-deoxy-D-xylulose-5-phosphate (DXP) and 3-amino-2-oxopropyl phosphate (1-amino-acetone-3-phosphate or AAP) to form pyridoxine 5'-phosphate (PNP) and inorganic phosphate

COG id: COG0854

COG function: function code H; Pyridoxal phosphate biosynthesis protein

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP synthase family

Homologues:

Organism=Escherichia coli, GI1788917, Length=233, Percent_Identity=56.2231759656652, Blast_Score=255, Evalue=2e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXJ_XANP2 (A7IM59)

Other databases:

- EMBL:   CP000781
- RefSeq:   YP_001418759.1
- ProteinModelPortal:   A7IM59
- SMR:   A7IM59
- STRING:   A7IM59
- GeneID:   5422392
- GenomeReviews:   CP000781_GR
- KEGG:   xau:Xaut_3878
- eggNOG:   COG0854
- HOGENOM:   HBG299314
- OMA:   AGHGLNY
- ProtClustDB:   PRK05265
- BioCyc:   XAUT78245:XAUT_3878-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00279
- InterPro:   IPR013785
- InterPro:   IPR004569
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00559

Pfam domain/function: PF03740 PdxJ; SSF63892 PyrdxlP_synth_PdxJ

EC number: =2.6.99.2

Molecular weight: Translated: 26963; Mature: 26832

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: NA

Important sites: ACT_SITE 48-48 ACT_SITE 75-75 ACT_SITE 199-199 BINDING 12-12 BINDING 23-23 BINDING 50-50 BINDING 55-55 BINDING 105-105 BINDING 200-200

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVLKPVRLGVNVDHVATVRNARGGALPDPVRAAALAKAAGAHGITAHLREDRRHIRDAD
CCCCCCEEECCCCHHHHHECCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
MERLKAEIDLPLNFEMAATDEMVAIACRVRPNACCLVPERREERTTEGGLDAAGQRAELA
HHHHHHHCCCCCCEEECCCCCEEEEEEEECCCEEEECCCHHHHCCCCCCCCCCCCHHHHH
PRIARLKAAGIRVSLFIAADPAQIAAAAELGADIVELHTGAWCDAVTEGRHVEAEAEFVR
HHHHHHHHCCEEEEEEEECCHHHHHHHHHHCCHHHHHCCCCHHHHHCCCCEEECHHHEEE
LKAGARQAAGLGLEVHAGHGLDYATAERIAAFPQIVELNIGHFLIGEAIFVGLDQAIARM
ECCCCHHHHCCCEEEECCCCCCHHHHHHHHHCCHHEEECCCHHHHHHHHHHHHHHHHHHH
RAAIAAGRAAVGDGAAA
HHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
AVLKPVRLGVNVDHVATVRNARGGALPDPVRAAALAKAAGAHGITAHLREDRRHIRDAD
CCCCCEEECCCCHHHHHECCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
MERLKAEIDLPLNFEMAATDEMVAIACRVRPNACCLVPERREERTTEGGLDAAGQRAELA
HHHHHHHCCCCCCEEECCCCCEEEEEEEECCCEEEECCCHHHHCCCCCCCCCCCCHHHHH
PRIARLKAAGIRVSLFIAADPAQIAAAAELGADIVELHTGAWCDAVTEGRHVEAEAEFVR
HHHHHHHHCCEEEEEEEECCHHHHHHHHHHCCHHHHHCCCCHHHHHCCCCEEECHHHEEE
LKAGARQAAGLGLEVHAGHGLDYATAERIAAFPQIVELNIGHFLIGEAIFVGLDQAIARM
ECCCCHHHHCCCEEEECCCCCCHHHHHHHHHCCHHEEECCCHHHHHHHHHHHHHHHHHHH
RAAIAAGRAAVGDGAAA
HHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA