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The map label for this gene is ubiF [C]

Identifier: 154247798

GI number: 154247798

Start: 4293091

End: 4294299

Strand: Direct

Name: ubiF [C]

Synonym: Xaut_3875

Alternate gene names: 154247798

Gene position: 4293091-4294299 (Clockwise)

Preceding gene: 154247794

Following gene: 154247800

Centisome position: 80.87

GC content: 71.71

Gene sequence:

>1209_bases
ATGGCGCAAGAGGACATCCACCACGGCGATACCCACCGTGACGACCTTCACGAGGTGATCGTGGTGGGCGCGGGTCCGGC
CGGGCTTGCCACCGCCCTGGGGCTCGCCGCGCGGGGCGTGCCCACGGCCCTTGTCACCGGACCCGATCGCGGGGCCGACC
ACCGCACCACGGCGCTGCTGGAAGGTTCGGTGCGCATCCTTGAGGATTTCGGCCTGTGGGCGGAGCTGGAGCCGCGCGCG
GCGCCGTTGCGCGACATGCGCATCGCCGATGCCACCCGCCGGCTTGTGCGGGCGCCGGAAGTCACCTTCCGGTCGGCGGA
AATCGGCCTGTCCACCTTCGGCTACAACATCGAGAACGATGCCCTGCGCAACGGCCTGCTTGCCAAGGCCATGCAGCAGC
CCAACCTTCACATCGCGCGCACCGCGCTGGAGCGCATCGAGTTTGCGGCCGACGCCCTTACCCTGCGTCTCGCGGACGGT
TCCATCCTGCGCGCCCGCCTGGTGGTGGGGGCGGATGGACGCCAGTCGCGGGTGCGGGCGGCGGCGGGCATCCAGATGCG
TACCCGGTCCTATCCGCAGGTGGCCTTCACCGCCACCGTGCGCCACACCCGCTCGCACGAGGACACCTCCACCGAATTCC
ACACCGAGACCGGTCCCTTCACCCTGGTGCCGCTGACGGGGGATCGTTCCTCCATCGTCTGCGTGGTCACGGAGCGGGAG
GCGATCCGTCTGCTCGGCCTCGACGACATGGCGCTGGCGCGGGAGCTGGAGGCGCGGGCCTCCTCCGTGCTCGGCCGCTT
CGAGATGGAGGGCGGCCGCGGCGCCTTCCCGCTGGCGGCGGAGACGGCGGAGCGGCTGGTGGCGGATCGCATCGCCCTCA
TCAGCGAGGCCGCCCACATCATGCCGCCCATCGGCGCGCAGGGCCTCAATCTCGGCCTGCGCGACGCAGGGGCATTGGTG
GCCTTCGTGGCGGATGCCTTCGCCGGGGGCGAGGACGTGGGCGGGGAGGCGCTGCTGTCGCGCTATGCGGCGGCGCGTCA
GCGCGACGTGTTCGGCCGCATGCGGGCGGTGGACCTGCTCAACCGTTCGCTCCTCTCGGACCTGCTGCCGATCCAGGGCG
TGCGCGGCTTCGGCCTCTATCTGCTCGACCGGGTGGGACCTCTCCGGCGCACGCTGATGCGGCTGGGCATCGGCGACAAG
GCGGCCTGA

Upstream 100 bases:

>100_bases
GGCATCTTGTGGCCCTGACTCGGGGCTGCGCCGCCTTTTCTCGGCCCCAAGCTGGTTCTAGTGTGCGCTGCGCCGGAGAC
ACGGCGGCGGGGAGGCTGCG

Downstream 100 bases:

>100_bases
CGCGTCGGCTCCGGGGGCCTCAGAACAGGCTGAGCGGGATGAGGTGCCCGCGCAGCAGATAGAGCATGCCGGTCACCGTC
ACCACCGAGAACAGGGTGCC

Product: UbiH/UbiF/VisC/COQ6 family ubiquinone biosynthesis hydroxylase

Products: 2-Octaprenyl-3-Methyl-5-Hydroxy-6-Methoxy-1,4- Benzoquinol [C]

Alternate protein names: NA

Number of amino acids: Translated: 402; Mature: 401

Protein sequence:

>402_residues
MAQEDIHHGDTHRDDLHEVIVVGAGPAGLATALGLAARGVPTALVTGPDRGADHRTTALLEGSVRILEDFGLWAELEPRA
APLRDMRIADATRRLVRAPEVTFRSAEIGLSTFGYNIENDALRNGLLAKAMQQPNLHIARTALERIEFAADALTLRLADG
SILRARLVVGADGRQSRVRAAAGIQMRTRSYPQVAFTATVRHTRSHEDTSTEFHTETGPFTLVPLTGDRSSIVCVVTERE
AIRLLGLDDMALARELEARASSVLGRFEMEGGRGAFPLAAETAERLVADRIALISEAAHIMPPIGAQGLNLGLRDAGALV
AFVADAFAGGEDVGGEALLSRYAAARQRDVFGRMRAVDLLNRSLLSDLLPIQGVRGFGLYLLDRVGPLRRTLMRLGIGDK
AA

Sequences:

>Translated_402_residues
MAQEDIHHGDTHRDDLHEVIVVGAGPAGLATALGLAARGVPTALVTGPDRGADHRTTALLEGSVRILEDFGLWAELEPRA
APLRDMRIADATRRLVRAPEVTFRSAEIGLSTFGYNIENDALRNGLLAKAMQQPNLHIARTALERIEFAADALTLRLADG
SILRARLVVGADGRQSRVRAAAGIQMRTRSYPQVAFTATVRHTRSHEDTSTEFHTETGPFTLVPLTGDRSSIVCVVTERE
AIRLLGLDDMALARELEARASSVLGRFEMEGGRGAFPLAAETAERLVADRIALISEAAHIMPPIGAQGLNLGLRDAGALV
AFVADAFAGGEDVGGEALLSRYAAARQRDVFGRMRAVDLLNRSLLSDLLPIQGVRGFGLYLLDRVGPLRRTLMRLGIGDK
AA
>Mature_401_residues
AQEDIHHGDTHRDDLHEVIVVGAGPAGLATALGLAARGVPTALVTGPDRGADHRTTALLEGSVRILEDFGLWAELEPRAA
PLRDMRIADATRRLVRAPEVTFRSAEIGLSTFGYNIENDALRNGLLAKAMQQPNLHIARTALERIEFAADALTLRLADGS
ILRARLVVGADGRQSRVRAAAGIQMRTRSYPQVAFTATVRHTRSHEDTSTEFHTETGPFTLVPLTGDRSSIVCVVTEREA
IRLLGLDDMALARELEARASSVLGRFEMEGGRGAFPLAAETAERLVADRIALISEAAHIMPPIGAQGLNLGLRDAGALVA
FVADAFAGGEDVGGEALLSRYAAARQRDVFGRMRAVDLLNRSLLSDLLPIQGVRGFGLYLLDRVGPLRRTLMRLGIGDKA
A

Specific function: Oxygenase That Introduces The Hydroxyl Group At Carbon Five Of 2-Octaprenyl-3-Methyl-6-Methoxy-1,4-Benzoquinol Resulting In The Formation Of 2-Octaprenyl-3-Methyl-5-Hydroxy-6-Methoxy-1,4- Benzoquinol. [C]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ubiH/COQ6 family [H]

Homologues:

Organism=Homo sapiens, GI32967305, Length=385, Percent_Identity=26.2337662337662, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI32967307, Length=329, Percent_Identity=26.7477203647416, Blast_Score=110, Evalue=3e-24,
Organism=Escherichia coli, GI1786883, Length=393, Percent_Identity=30.0254452926209, Blast_Score=158, Evalue=7e-40,
Organism=Escherichia coli, GI1789273, Length=393, Percent_Identity=30.0254452926209, Blast_Score=149, Evalue=4e-37,
Organism=Escherichia coli, GI1789274, Length=393, Percent_Identity=30.0254452926209, Blast_Score=140, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI25146631, Length=435, Percent_Identity=25.9770114942529, Blast_Score=126, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6321694, Length=286, Percent_Identity=22.7272727272727, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19920760, Length=397, Percent_Identity=25.1889168765743, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042
- InterPro:   IPR018168
- InterPro:   IPR010971 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.- [C]

Molecular weight: Translated: 43190; Mature: 43059

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQEDIHHGDTHRDDLHEVIVVGAGPAGLATALGLAARGVPTALVTGPDRGADHRTTALL
CCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHEEE
EGSVRILEDFGLWAELEPRAAPLRDMRIADATRRLVRAPEVTFRSAEIGLSTFGYNIEND
CCCHHHHHHCCCCEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEHHHCCHHHCCCCCCHH
ALRNGLLAKAMQQPNLHIARTALERIEFAADALTLRLADGSILRARLVVGADGRQSRVRA
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHEEEEEECCCCEEEEEEEECCCCCHHHHHH
AAGIQMRTRSYPQVAFTATVRHTRSHEDTSTEFHTETGPFTLVPLTGDRSSIVCVVTERE
HHCCEEECCCCCCEEEEEEHHHCCCCCCCCCCEECCCCCEEEEEECCCCCCEEEEEECCC
AIRLLGLDDMALARELEARASSVLGRFEMEGGRGAFPLAAETAERLVADRIALISEAAHI
EEEEECCCHHHHHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
MPPIGAQGLNLGLRDAGALVAFVADAFAGGEDVGGEALLSRYAAARQRDVFGRMRAVDLL
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
NRSLLSDLLPIQGVRGFGLYLLDRVGPLRRTLMRLGIGDKAA
HHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
AQEDIHHGDTHRDDLHEVIVVGAGPAGLATALGLAARGVPTALVTGPDRGADHRTTALL
CCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHEEE
EGSVRILEDFGLWAELEPRAAPLRDMRIADATRRLVRAPEVTFRSAEIGLSTFGYNIEND
CCCHHHHHHCCCCEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEHHHCCHHHCCCCCCHH
ALRNGLLAKAMQQPNLHIARTALERIEFAADALTLRLADGSILRARLVVGADGRQSRVRA
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHEEEEEECCCCEEEEEEEECCCCCHHHHHH
AAGIQMRTRSYPQVAFTATVRHTRSHEDTSTEFHTETGPFTLVPLTGDRSSIVCVVTERE
HHCCEEECCCCCCEEEEEEHHHCCCCCCCCCCEECCCCCEEEEEECCCCCCEEEEEECCC
AIRLLGLDDMALARELEARASSVLGRFEMEGGRGAFPLAAETAERLVADRIALISEAAHI
EEEEECCCHHHHHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
MPPIGAQGLNLGLRDAGALVAFVADAFAGGEDVGGEALLSRYAAARQRDVFGRMRAVDLL
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
NRSLLSDLLPIQGVRGFGLYLLDRVGPLRRTLMRLGIGDKAA
HHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: @OCTA02.txt*2-Octaprenyl-3-Methyl-6-Methoxy-1,4-Benzoquinol! [C]

Specific reaction: @OCTA02.txt*2-Octaprenyl-3-Methyl-6-Methoxy-1,4-Benzoquinol! = 2-Octaprenyl-3-Methyl-5-Hydroxy-6-Methoxy-1,4- Benzoquinol [C]

General reaction: Hydroxylation [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]